王不留行环肽研究

从中药王不留行（Ｖａｃａｒｉａｓｅｇｅｔａｌｉｓ）种子中分离并鉴定了４个环肽化合物,分别命名为王不留行环肽Ａ,Ｂ,Ｃ,Ｄ（ｖａｃｃａｒｉｎｓＡ,Ｂ,Ｃ,Ｄ）,其中王不留行环肽Ａ为新环肽化合物。其结构通过光谱和化学方法分别确定为：ｖａｃｃａｒｉｎＡ——ｃｙｃｌｏ－（Ｔｒｐ－Ａｌａ１－Ｇｌｙ－Ｖａｌ－Ａｌａ２）,ｖａｃｃａｒｉｎＢ———ｃｙｃｌｏ－（Ｐｒｏ－Ｇｌｙ－Ｌｅｕ－Ｓｅｒ－Ｐｈｅ１－Ａｌａ－Ｐｈｅ２）,ｖａｃｃａｒｉｎＣ———ｃｙｃｌｏ－（Ｐｒｏ１－Ｇｌｙ－Ｔｙｒ－Ｖａｌ－Ｐｒｏ２－Ｌｅｕ－Ｔｒｐ）,ｖａｃａｒｉｎＤ———ｃｙｃｌｏ－（Ｐｒｏ－Ｖａｌ１－Ｔｒｐ－Ａｌａ－Ｇｌｙ－Ｖａｌ２）．     

云南锡叶藤属一新种

勐腊锡叶藤　新种　图１ＴｅｔｒａｃｅｒａｘｕｉＨ－ＺｈｕｅｔＨ－Ｗａｎｇ,ｓｐ－ｎｏｖ－ｐｌ－ＡｆｆｉｎｉｓＴ－ｓｃａｎｄｅｎｔｉ（Ｌｉｎｎ－）Ｍｅｒｒ－ｓｅｄｉｎｆｌｏｒｅｓｃｅｎｔｉｉｓｍｉｎｏｒｉｂｕｓ,ｐａｕｃｉｆｌｏｒｉｓ,ｓｅｐａｌｉｓｍａｊｏｒｉｂｕｓ１－５ｃｍｌｏｎｇｉｓｉｎｓｔａｔｕｆｒｕｃｔｕｅｘｔｕｓｄｅｎｓｅｖｉｌｌｏｓｉｓ,ｓｔａｍｉｎｉｂｕｓ１ｃｍｌｏｎｇｉｓ,ｃａｐｓｕｌｉｓｍａｊｏｒｉｂｕｓｈｉｒｓｕｔｉｓ,１－５～２ｃｍｌｏｎｇｉｓ,１－２～１－５ｃｍｌａｔｉ…     

c—fos,c—myb和c—erbB—2与大鼠卵巢颗粒细胞生孕酮调？…

目的和方法：用离体细胞体外孵育法,观察肥义ｃ－ｆｏｓ、ｃ－ｍｙｂ和ｃ－ｅｒｂＢ－２寡脱氧核苷酸（ｃ－ｆｏｓ ＯＤＮ、ｃ－ｍｙｂ ＯＤＮ和ｃ－ｅｒｂＢ－２ ＯＤＮ）对ｈＣＧ诱导大鼠颗粒细胞孕酮产生的影响。同时观察内皮素－１、γ－氨基丁酸和钙离子通道阻断剂维拉帕米对细胞中ｃ－ｆｏｓ、ｃ－ｍｙｂ和ｃ－ｅｒｂＢ－２蛋白的影响。结果：反义ｃ－ｆｏｓ、ｃ－ｍｙｂ和ｃ－ｅｒｂＢ－２ ＯＤＮ均明显抑制ｈＣＧ诱导颗     

云衫针层孔菌化学成分的研究

从云衫针层孔菌Ｐｈｅｌｌｉｎｕｓ ｙａｍａｎｏｉ（Ｔａｍｚ．）ｓｈａｗ分离得到１２个化合物,借助光谱分析鉴定为：８,１１,１３－ａｂｉｅｔａｄｉｅｎ－１８－ｏｉｃ ａｃｉｄ（１）,１,２－ｂｅｎｚｅｎｅｄｉ－ｃａｒｂｏｘｙｌｉｃ ａｃｉｄｂｉｓ（２－ｅｔｈｙｌ－ｈｅｘｙｌ）ｅｓｔｅｒ（２）,ｅｒｇｏｓｔｅｒｏｌ ｐｅｒｏｘｉｄｅ（３）,ｅｒｇｏｓｔａ ７,２２（Ｅ）－ｉｄｅｎ－３－ｏｌ（４）,ｅｒｇｏｓｔａ ７,２２（Ｚ）－ｄｉｅｎ－３－ｏｌ（５）,ｏｃｔａｄｅｃａｎｏｉｃ ａｃｉｄ－１,３－ｐｒｏｐａｎｅｄｉｙｌ ｅｓｔｅｒ（６）,ｏｃｔａｄｅｃａｎｏｉｃ ａｃｉｄ（７）,８（１９）,１４－ｌａｂｄａｄｉｅｎ－１３－ｏｌ（８）,ｅｒｇｏｓｔａ－４,６,８（１４）,２２－ｔｅｔｒａｅｎ－３－ｏｎｅ（９）,化合     

豚鼠气道及肺组织原癌基因表达与哮喘的相关研究

目的：为研究原癌基因在哮喘发病中的作用。方法：以卵白蛋白致敏豚鼠建立哮喘模型,用Ｄｏｔ－ｂｌｏｔ、Ｎｏｒｔｈ－ｅｒｎ－ｂｌｏｔ分子杂交及免疫组化技术,分别观察正常及哮喘发作后豚鼠气道上皮及肺组织中原癌基因ｃ－ｆｏｓ、ｃ－ｍｙｃ的表达水平。结果：正常豚鼠气道及肺组织中ｃ－ｆｏｓ及ｃ－ｍｙｃｍＲＮＡ无或很少表达,哮喘发作后豚鼠气道上皮及肺组织中ｃ－ｆｏｓ和ｃ－ｍｙｃｍＲＮＡ表达明显增强,３０ｍｉｎ达高峰,发作后４ｈ降至正常水平。地塞米松对ｃ－ｆｏｓ及ｃ－ｍｙｃｍＲ－ＮＡ有部分抑制作用。结论：原癌基因ｃ－ｆｏｓ及ｃ－ｍｙｃ在哮喘发病过程中可能起一定作用。     

非致病微生物在植物—病原菌协同进化中的作用

非致病微生物在植物－病原菌协同进化中的作用单卫星（西北农业大学植保系,陕西杨陵７１２１００）ＴｈｅＲｏｌｅｏｆＮｏｎ－ｐａｔｈｏｇｅｎｉｃＭｉｃｒｏｏｒｇａｎｉｓｍｓｉｎＰｌａｎｔ－ｐａｔｈｏｇｅｎＣｏｅｖｏｌｕｔｉｏｎ￥ＳｈａｎＷｅｉｘｉｎｇ（Ｄｅｐａｒｔ－ｍｅｎｔｏｆＰｌａｎｔＰｒｏｔｅｃｔｉｏｎ,ＮｏｒｔｈｗｅｓｔｅｒｎＡｇｒｉｃｕｌｔｕｒａｌＵｎｉｖｅｒｓｉｔｙ,Ｙａｎｇｌｉｎｇ,Ｓｈａａｎｘｉ７１２１００）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ,１９９３,１２（６）,５４－５９．Ｉｎｖｉｅｗｏｆｐｒｅｓｅｎｔｐｒｏｂｌｅｍｓａｓｓｏｃｉａｔｅｄｗｉｔｈｐｌａｎｔｄｉｓｅａｓｅｃｏｎｔｒｏｌ,ｃｏｕｐｌｅｄｗｉｔｈｒｅｃｅｎｔａｄｖａｎｃｅｓｉｎｂｉｏｌｏｇ－ｉｃａｌｃｏｎｔｒｏｌｏｆｐｌａｎｔｄｉｓｅａｓｅｓａｎｄｐｌａｎｔ－ｐａｔｈｏｇｅｎｉｎｔｅｒａｃｔｉｏｎｓ,ｔｈｅａｕｔｈｏｒｐｏｉｎｔｅｄｏｕｔｔｈｅｉｍｐｏｒｔａｎｃｅｏｆｎｏｎ－ｐａｔｈｏｇｅｎｉｃｍｉｃｒｏｏｒｇａｎｉｓｍｓｉｎｈａｂｉｔｉｎｇｐｌａｎｔｓｕｒｆａｃｅｓｉｎｒｅｇｕｌａｔｉｏｎｏｆｐｌａｎｔ－ｐａｔｈｏｇｅ     

几丁质酶和壳聚糖酶对部分乙酰化壳聚糖作用方式的比较

通过对几丁质酶和壳聚糖酶降解部分乙酰化壳聚糖的作用方式的比较,得到几丁质酶切断壳聚糖的ＧｌｃＮＡｃ－ ＧｌｃＮＡｃ 和ＧｌｃＮＡｃ－ ＧｌｃＮ 或ＧｌｃＮ－ ＧｌｃＮＡｃ 糖苷键,而壳聚糖酶切断壳聚糖的ＧｌｃＮ－ ＧｌｃＮ 和ＧｌｃＮ－ ＧｌｃＮＡｃ 或ＧｌｃＮＡｃ－ ＧｌｃＮ 糖苷键,为得到较高聚合度的壳寡糖提供理论基础。     

洞庭湖滨湖湿地平原农村生态工程初步研究

洞庭湖滨湖湿地平原农村生态工程初步研究严斧,游有林,刘劲凡,卢源德,周为民（中国科学院生态环境研究中心系统生态室沅江课题组,大庸市４１６６００）ＲｕｒａｌＥｃｏｌｏｇｉｃａｌＥｎｇｉｎｅｅｒｉｎｇｏｎＷｅｔｌａｎｄＰｌａｉｎｏｆＤｏｎｇｔｉｎｇＬａｋｅｓｉｄｅ￥．ＹａｎＦｕ;ＹｏｕＹｏｕｌｉｎ;ＬｉｕＪｉｎｆａｎ;ＬｕＹｕａｎｄｅ;ＺｈｏｕＷｅｉｍｉｎ（ＹｕａｎｊｉａｎｇＲｅｓｅａｒｃｈＧｒｏｕｐ,ＲｅｓｅａｒｃｈＣｅｎｔｅｒｆｏｒＥｃｏ－ｅｎｖｉｒｏｎｍｅｎｔａｌＳｃｉｅｎｃｅ,Ａ－ｃａｄｅｍｉａＳｉｎｉｃａ,Ｄａｙｏｎｇ４１６６００）．ＣｈｉｎｅｓｅＪｏｕｒｎａｌｏｆＥｃｏｌｏｇｙ,１９９３,１２（２）：４５—４８．ＡｃｃｏｒｄｉｎｇｔｏｔｈｅｍａｉｎｔｙｐｅｓｏｆｒｅｓｏｕｒｃｅｃｏｍｂｉｎａｔｉｏｎｓｏｎｗｅｔｌａｎｄｐｌａｉｎｏｆＤｏｎｇｔｉｎｇｌａｋｅｓｉｄｅ,ｓｅｖｅｒａｌｅｃｏ－ｌｏｇｉｃａｌｅｎｇｉｎｅｅｒｉｎｇｓｉｎｃｌｕｄｉｎｇｌａｋｅｓｉｄｅｒｅｅｄ－ｆｏｒｅｓｔ,ｌｏｗｌａｎｄｒｉｃｅ－ｆｉｓｈ－ｒａｍｉｅ,ｒｎａｎｕｒｅ－ｆｏｒａｇｅ－ｆｕｅｌ,ｆｉｓｈ－ｐｉｇ－ｄｕｃｋａｎｄｆ     

血管紧张素Ⅱ诱导左心室原癌基因c－fos和c－myc的表达

本实验用Ｌａｎｙｅｎｄｏｒｆｆ心脏灌流装置,探讨血管紧张素Ⅱ对左心室原癌基因ｃ－ｆｏｓ和ｃ－ｍｙｃ表达的作用。观察到血管紧张素Ⅱ能诱导左心室ｃ－ｆｏｓ和ｃ－ｍｙｃ的表达,ｃ－ｆｏｓ表达早于ｃ－ｍｙｃ表达,并呈量－效关系。这种作用可被血管紧张素Ⅱ受体拮抗剂ｓａｒａｌａｓｉｎ所阻断。这些结果提示血管紧张素Ⅱ诱导的ｃ－ｆｏｓ和ｃ－ｍｙｃ的表达是受体介导的。ＴＴＸ完全阻断血管紧张素Ⅱ诱导的ｃ－ｆｏｓ表达。ＴＴＸ对ｃ－ｍｙｃ的表达无明显影响。     

Forskolin对放射性转化细胞TGF基因及部分癌基因表达的影响

＾３Ｈ－ＴｄＲ放射性转化细胞经１×１０＾－５ｍｏｌ／Ｌ Ｆｏｓｋｏｌｉｎ处理２４ｈ后,ＴＧＦａ,ｃ－ｍｙｃ,ｃ－Ｋ－ｒａｓ基因的ｍＲＮＡ表达下降;ＴＧＦβ,ｃ－ｆｏｓ基因表达无明显变化。非转化细胞经相同条件处理,ＴＧＦａ,ＴＧＦβ,ｃ－ｍｙｃ及ｃ－Ｋ－ｒａｓ基因表达无显著改变。提示：Ｆｏｒｓｋｏｌｉｎ介导的转化细胞的生长抑制作用与ＴＧＦａ,ｃ－ｍｙｃ,ｃ－Ｋ－ｒａｓ基因的转录表达下降有关。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.