Meeting review: a summary of the Label-Free Summit

‘All our knowledge has its origins in our perceptions.’ Leonardo da Vinci

Scientific progress is often enabled by the development of new tools and technologies that have given us new ways of perceiving the world. In the early days of our science, optical microscopy gave us the ability to observe cells for the first time and opened the new world of cell biology. More recently, advances in cloning and labeling technologies have permitted us to study the interactions of individual proteins. Now, label-free detection technology provides another promising advance – the means to generically study signal transduction in living cells through the dynamic mass redistribution (DMR) of intracellular contents. On October 6–7, 2008 a group of researchers gathered in Corning, NY to share recent advances in the field of label-free detection. Attendees came from nearby Ithaca, NY and as far away as Tokyo, Japan, representing a diverse set of institutions engaged in drug discovery research. Topics ranged from seven transmembrane receptor (7TMR) signaling, to high throughput screening and profiling, and to new applications such as ion channels and viral infection assays. Overall, the Label-Free Summit has given us additional perspective on the potential of this promising technology.     

RNA interference in plant parasitic nematodes: a summary of the current status

SUMMARYRNA interference (RNAi) has emerged as an invaluable gene-silencing tool for functional analysis in a wide variety of organisms, particularly the free-living model nematode Caenorhabditis elegans. An increasing number of studies have now described its application to plant parasitic nematodes. Genes expressed in a range of cell types are silenced when nematodes take up double stranded RNA (dsRNA) or short interfering RNAs (siRNAs) that elicit a systemic RNAi response. Despite many successful reports, there is still poor understanding of the range of factors that influence optimal gene silencing. Recent in vitro studies have highlighted significant variations in the RNAi phenotype that can occur with different dsRNA concentrations, construct size and duration of soaking. Discrepancies in methodology thwart efforts to reliably compare the efficacy of RNAi between different nematodes or target tissues. Nevertheless, RNAi has become an established experimental tool for plant parasitic nematodes and also offers the prospect of being developed into a novel control strategy when delivered from transgenic plants.     

Large saline lakes of former USSR: a summary review

Shoot biomass and nitrogen, accumulated within above-ground plant biomass in autumn, correlate with nitrogen availability indicated by nitrogen content (% DM) of several shoot parts during the height of the growth period.A higher nitrogen percentage of the shoots is correlated with a higher shoot loss and subsequent substitution by branching and tillering during and continuing toward the end of the growth period. A delayed switch from the vegetative to the generative phase reduces the translocation of reserve material to the rhizome. Increasing nitrogen load is at least one of the factors causing instability and reed-belt decline.     

Hyperhidrosis: a review of current management

Primary hyperhidrosis is a troublesome disorder of excessive perspiration that affects as much as 1 percent of the population. Sufferers are usually young and are often affected by related social, professional, and psychological problems. Many methods for treating hyperhidrosis exist; however, no single treatment is without its weakness or complications. This article aims to clarify the issues related to the use of each treatment modality, including the most recently proposed method using botulinum toxin.     

Interactions between imprinting effects: summary and review

Mice with uniparental disomies (uniparental duplications) for defined regions of certain chromosomes, or certain disomies, show a range of developmental abnormalities most of which affect growth. These defects can be attributed to incorrect dosages of maternal or paternal copies of imprinted genes lying within the regions involved. Combinations of certain partial disomies result in interactions between the imprinting effects that seemingly independently affect foetal and/or placental growth in different ways or modify neonatal and postnatal development. The findings are generally in accord with the 'conflict hypothesis' for the evolution of genomic imprinting but do not demonstrate common growth axes within which imprinted genes may interact. Instead, it would seem that any gene that favours embryonic/foetal development, at consequent cost to the mother, will have been subject to evolutionary selection for only paternal allele expression. Reciprocally, any gene that reduces embryonic/foetal growth to limit disadvantage to the mother will have been selected for only maternal allele expression. It is concluded that survival of the placenta is core to the evolution of imprinting.     

Recent developments in genomewide association scans: a workshop summary and review

With the imminent availability of ultra-high-volume genotyping platforms (on the order of 100,000-1,000,000 genotypes per sample) at a manageable cost, there is growing interest in the possibility of conducting genomewide association studies for a variety of diseases but, so far, little consensus on methods to design and analyze them. In April 2005, an international group of >100 investigators convened at the University of Southern California over the course of 2 days to compare notes on planned or ongoing studies and to debate alternative technologies, study designs, and statistical methods. This report summarizes these discussions in the context of the relevant literature. A broad consensus emerged that the time was now ripe for launching such studies, and several common themes were identified--most notably the considerable efficiency gains of multistage sampling design, specifically those made by testing only a portion of the subjects with a high-density genomewide technology, followed by testing additional subjects and/or additional SNPs at regions identified by this initial scan.     

Biological effects of oxidized phytosterols: a review of the current knowledge

The cholesterol-lowering effect of phytosterols has been extensively studied, and consumption of phytosterols is among the recommendations to lower LDL-cholesterol concentrations. Due to their structural similarity with cholesterol, phytosterols may undergo oxidative processes comparable to those involved in cholesterol oxidation. Consumption of phytosterols could therefore lead to increased systemic concentrations of oxidized phytosterols (oxyphytosterols) via increased dietary intake or in vivo formation from non-oxidized phytosterols. While the biological effects of oxidized cholesterol (oxycholesterol) have been well studied, the amount of biological research on oxyphytosterols is scarce. Most reports on oxyphytosterols cover their quantitative analysis. Whether oxyphytosterols may play similar biological roles as compared to oxycholesterol has not been fully elucidated. The usual perception about oxyphytosterols is that these components present a concern in terms of food quality and health. This perception originates from the parallel that is made with oxycholesterol. Yet, in line with results for oxycholesterol, recent data suggest that oxyphytosterols--depending on the type of oxidation product--may also have beneficial biological properties. Therefore, the objective of this review is to summarise the current understanding of the biological effects, next to identifying future research needs that will help to clarify the possible impact of oxyphytosterols on human health.     

Xanthelasma palpebrarum: a review and current management principles

LEARNING OBJECTIVES: After studying this article, the participant should be able to: 1. Describe the differential diagnosis of xanthelasma palpebrarum. 2. Discuss the various nonsurgical and surgical treatment options and their advantages and disadvantages. 3. Describe the circumstances in which recurrence is most likely after treatment. Xanthelasma palpebrarum is the most common cutaneous xanthoma. It typically presents in middle-aged and older adults, most often around the eyelids. The diagnosis can often be made on clinical grounds alone. For the plastic surgeon, it is important to apply an algorithmic approach to the treatment of these lesions. Depending on the size and location, several different methods can be used to address this problem, ranging from simple excision, to laser treatment, to chemical peeling. This article addresses the underlying pathophysiology of xanthelasma and the currently accepted modes of treatment.     

Connecting cytokines and brain: a review of current issues

Cytokines have been a multi-disciplinary research focus for over 2 decades. To date, there have been more than 15,000 articles published concerning the relationship between cytokines and the central nervous system (CNS). Over half of these articles have been published in the last 5 years. From such vast number of studies, two major topics emerge as the critical issues: 1) how do cytokines modulate the functions of the CNS? 2) what is the role of cytokines in the pathogenesis of neurological diseases? Thus far, it has been clearly established that cytokines can alter the functions of the CNS in specific manners, invoking CNS-controlled autonomic, neuroendocrine, and behavioral responses. Induced expression of cytokines has also been found in the CNS during brain injury and infection, contributing to the immunological processes at this "immunologically privileged" site. Furthermore, increasing evidence points to the potential involvement of cytokines in the induction and modulation of an array of neurological diseases ranging from Alzheimer's disease to chronic fatigue syndrome. Despite such progress, however, substantial obstacles remain for both the basic understanding and the potential clinical exploitation of how cytokines interact with CNS. In this review, we will attempt to synopsize the current theories and evidence regarding the answers to the above-mentioned critical questions. These issues will be reviewed not only in isolation, as most of the original reports focused on only one of the questions, but also in parallel such that inter-issue insights may be gained.     

Theorizing transnational immigration: a critical review of current efforts

During the past decade, transnationalism has entered the lexicon of migration scholars. As with other terms used in the study of immigration and ethnicity, this concept suffers from ambiguity as a result of competing definitions that fail to specify the temporal and spatial parameters of the term and to adequately locate it vis-à-vis older concepts such as assimilation and cultural pluralism. This article offers a review and critique of the ways the term has come to be employed at the hands of key spokespersons that have articulated the most sustained theoretical rationales to date for transnationalism as a conceptual construct to account for new immigrant identities and communities. The conclusion of the essay offers in schematic form an alternative assessment of transnationalism that locates it as one potential subset of assimilation theory, rather than as an alternative to it.     

Hemolytic disease of the newborn: a review of current trends and prospects

This study aims to investigate the clinical application value of serum total bilirubin, hemoglobin (Hb) and reticulocyte percentage (Ret%) combined with immunohematological tests in the diagnosis of ABO hemolytic disease of the newborn (ABO-HDN). A retrospective analysis of 503 neonatal blood samples of mothers with RhD(+) blood type O and neonates with RhD(+) blood type A/B, admitted to the Third Xiangya Hospital of Central South University from March 2020 to September 2021, was conducted. HDN was investigated with the elution test, indirect antiglubin test (IAT), and direct antiglubin test (DAT). The positive rate of the immunohematological tests for neonatal hemolysis, combined with the detection of serum total bilirubin, Hb and Ret%, was significantly higher than that of immunohematological tests for neonatal hemolysis alone (P<0.05). The elution test can be used as a gold standard to diagnose HDN. Neonates with blood type A had a higher probability of ABO hemolysis than those with blood type B. This study suggests that serum bilirubin, Hb, and Ret% detection combined with immunohematological tests can improve the detection rate of ABO-HDN.     

Cryopreservation of hepatocytes: a review of current methods for banking

Cryopreservation, the freezing of hepatocytes in liquid nitrogen for storage, has been investigated for many years, as a method of long-term storage for hepatocytes. Unfortunately an agreed acceptable protocol has been elusive, in part due to the susceptibility of hepatocytes to the freeze thaw process involved. A method for long-term storage (months, possibly years) of human hepatocytes, in particular, is desirable for the development of a clinically applicable bioartificial liver, hepatocyte transplantation and for pharmacotoxicological research. The sources of human liver tissue from which hepatocytes can be derived are limited. Many groups have modified and improved the process of cryopreservation and many new techniques have been published, including the incorporation of such cryopreserved cells in clinically based studies. Further evaluation is still required to develop a universally acceptable protocol. This article reviews the difficulties involved in cryopreserving hepatocytes for banking and examines recent technical advances within this field.