Geniculate relay of interhemispherical transmission of visual information

Electrophysiological experiments were made on conscious cats with different degree of dissection of the classical and commissural optic tracts to the lateral geniculate body. The data obtained indicated that dissection of the direct retinal tracts to the lateral geniculate body (unilateral dissection of the optic tract) leads merely to the reduction in the amplitude of evoked potentials in response to light flashes. However, the latent period of the first component of the response in this structure appeared the same as in intact animals. Comparison of these data with those obtained in analogous experiments made on the visual cortex allows the conclusion that the geniculate body is the main subcortical relay of the visual commissural effects which are transmitted from the retina to the cortical regions of the brain.     

Interhemispheric transmission of visual information: behavioral and electrophysiologic aspects

Efficiency of callosal and extracallosal channels of interhemispheric transfer of visual information was studied by behavioural and electrophysiological methods on cats with sections of classical and commissural pathways of the visual system. The results showed a high ability of the operated animals to form conditioned defensive reflex to visual stimuli. The primary complexes of evoked potentials to light flashes in the projection cortex of both hemispheres were also preserved. The data show the significant role of the callosal and extracallosal pathways of the transmission of visual information across the midline.     

Effect of deafferentation of the lateral geniculate body on its background activity

Elimination of reticular inputs to the lateral geniculate body (LGB) by sectioning of one half of the midbrain operculum, did not affect significantly the characteristics of the LGB evoked potential to light stimulus. At the same time LGB response to stimulation of the reticular formation by a single current impulse, though did not disappear completely, but changed greatly: its latency became twice as long, the negative component of the response was no more recorded. In conditions of LGB deafferentation, the characteristics of all rhythms of its electrical activity, besides the alpha-like one, considerably changed. At the same time, exactly this last rhythm underwent the greatest changes on the EEG of the visual cortex. On the basis of the obtained data it is suggested that the reticular formation takes a considerable and multiple part in generation of LGB rhythmic activity and that changes in its characteristics are clearly reflected in the ECoG rhythms formation. Retention of the LGB visual evoked potential and of the response to stimulation of the reticular formation after the section of one half of the midbrain operculum testifies to the presence of several reticular inputs to LGB.     

Role of the neocortical and subcortical commissures in the interhemispheric transfer of visual information to the hippocampus

The light flash evoked potentials were recorded in the hippocamp of cats with transected left optic tract and cerebral commissures. The data obtained show a significant reduction in the response amplitude (by 1.5-2 times) in both hemispheres after unilateral transection of the optic tract. The minimal response amplitude (10-15 times less than normal) was recorded in the left hippocamp of cats with additional transection of the commissures of the endbrain, mesencephalon and diencephalon. In contrast, the latent period of the first and second components of the evoked potential was similar to that in the control group animals. It is suggested that the neocortical commissural visual inputs play the most important role in interhemispheric transmission of visual information to the hippocamp.     

Reticular activation by splanchnic afferents: its influence on visual projections at the level of the cerebral cortex in cats]

In an previous work, we have shown a strong increase of visual evoked potentials of the medial lateral gyrus by splanchnic stimulation. This present study demonstrates that this effect is due to the activation of the substantia reticularis. Actually, we observed: 1) an increase of the reticular visual potential by splanchnic stimulus in convergent zone; 2) an increase of the visual evoked potential in the primary cortical area by stimulation of this reticular formation. These results show the existence of a functional pathway including the splanchnic nerve, the reticular formation and the visual cortex.     

Effect of strychnine on the neuronal reactivity of the sensorimotor cortex and the temporary connection in rabbits

On awake nonimmobilized rabbits, evoked activity was studied of the sensorimotor cortex neurons in response to stimulation of the pyramidal tract, medial lemniscus and reticular nucleus of the midbrain tegmentum by stimuli of different frequencies, and driving reaction of cortical neurons to stimulation of these brain structures by series of stimuli of increasing frequency. Conditioned reflexes were also studied, established on the basis of combination of direct stimulation of the sensorimotor cortex and electrocutaneous stimulation. Application of the cortex of low concentration of strychnine solutions (less than 1%) heightened neurons reactivity and provides for the formation of temporary connection. Application of strychnine solutions of higher concentration (greater than 1%) led to opposite effects. Interconnection of electrical and behavioural effects is discussed.     

Comparative analysis of the role of the second and first somatosensory cortical areas in somatic responses of the medullary reticular formation in rats

Evoked potentials and unit activity in the medullary reticular formation were investigated in unanesthetized, curarized rats during cold blocking or after extirpation of the cortical representation of one of the stimulated limbs. Local cooling or extirpation in area SII, unlike blocking of area SI, leads to a small (up to 30%) decrease in amplitude and a very small change (up to 10 msec) in the temporal parameters of evoked potentials arising in the reticular formation in response to electrodermal stimulation of the contralateral limb, whose representation in the cortex was blocked. Predominance of corticofugal influences from SI over those from SII was discovered both in experiments with evoked potentials and during analysis of somatic spike responses of reticular formation neurons. Corticofugal control over activity of the medullary reticular formation in rats exerted by neuronal mechanisms of somatosensory areas SII and SI thus differs both qualitatively and quantitatively.I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 15, No. 1, pp. 42–49, January–February, 1983.     

Effects of repetitive stimulation of the locus coeruleus on spinal inhibitory responses to suprasegmental stimulation in cats

Effects of repetitive stimulation of the locus coeruleus on spinal responses to activation of cortico-, reticulo-, and vestibulospinal tracts were studied in decerebellate cats anesthetized with chloralose. Descending influences of these structures were assessed from changes in amplitude of extensor and flexor monosynaptic discharges or from the magnitude of postsynaptic potentials recorded from the corresponding motoneurons. Stimulation of the motor cortex or modullary reticular formation as a rule evoked two-component inhibitory responses in extensor motoneurons and excitatory-inhibitory responses in flexor motoneurons. Stimulation of locus coeruleus effectively depressed the amplitude of the late component and, to a lesser degree, that of the early component of inhibition arising after stimulation of the cerebral cortex or reticular formation. During stimulation of the locus coeruleus no marked changes were found in inhibitory responses evoked by vestibulospinal influences in flexor motoneurons, and also in excitatory responses arising after stimulation of the above-mentioned descending pathways in both groups of motoneurons.     

Effect of local cooling of the cortex on evoked potentials in the reticular formation in response to somatosensory stimulation

Potentials evoked in nuclei of the reticular formation by electrodermal stimulation of the limbs were investigated in acute experiments on unanesthetized, immobilized rats during cooling of the somatosensory cortex in the area of representation of one forelimb. Evoked potentials in the reticular formation were found to depend on the degree of cold inhibition of the cortical primary response to the same stimulation. The peak time of the main negative wave increased from 40–50 to 60–80 msec with a simultaneous decrease in its amplitude or its total disappearance in the case of deep cooling of the cortex. Cooling of the cortex had a similar although weaker effect on the earlier wave of the evoked potential with a peak time of 14 msec, recorded in the ventral reticular nucleus. In parallel recordings of potentials evoked by stimulation of other limbs they remained unchanged at these same points of the reticular formation or were reduced in amplitude while preserving the same temporal parameters. Cooling of the cortex thus selectively delays the development and reduces the amplitude of the response to stimulation of the limb in whose area of representation transformation of the afferent signal into a corticofugal volley is blocked. Consequently the normal development of both late and early components of the potential evoked in the reticular formation by somatic stimulation requires an additional volley, descending from the cortex, and formed as a result of transformation of the same afferent signal in the corresponding point of the somatosensory cortex.I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Translated from Neirofiziologiya, Vol. 13, No. 1, pp. 32–38, January–February, 1981.     

Extracallosal channels of interhemispheric transmission of visual information

Evoked potentials (EP) in response to light flashes were recorded in cats with different degree of optical tract disconnection. In intact and operated on animals, the latent period of the first component of these EP was the same as in the visual cortex. The different degree of disconnection of the classic and commissural optical tract brought about an increase in the amplitude of commissural potentials. The data obtained point to the existence of the effective callosal and extracallosal volleys of interhemispheric transmission of visual information, which are also likely to pay an essential part in compensatory processes of the optical system.     

Organization of neuronal responses in the sensomotor cortex during simultaneous stimulation of brain structures

It has been found on alert non-immobilized rabbits that paired stimulations of the medical lemniscus, the midbrain reticular formation and the pyramidal tract in different succession cause in the sensorimotor cortex the formation of a temporary connection by the parameters of single unit activity and evoked potentials. The temporary connection is manifested in dynamics of evoked responses and in the change of electrical activity at the moment of usual presentation of the second stimulus, if it is omitted. The inflow of impulse activity by different paths adds some peculiarities to the organization of the temporary connection. The leading role in the given model situation may belong to the non-specific system.     

Analysis of long cortical evoked potentials

Evoked potentials arising in the motor cortex in response to its direct stimulation (dendritic and slow negative potentials), to stimulation of the ventrolateral (primary response) and intralaminar (nonspecific response) thalamic nuclei, and to stimulation of the pyramidal tracts (antidromic response), and also postsynaptic responses of neurons corresponding to them were studied in acute experiments on curarized cats. Evoked potentials arising in response to direct cortical stimulation and also to stimulation of the specific and nonspecific thalamic nuclei and pyramidal tracts were recorded from the same point of the motor cortex, and the corresponding intracellular responses were recorded from the same neuron. Slow negative potentials arising under these conditions of stimulation and the IPSPs corresponding to them were shown to have an identical time course. The results show that slow negative potentials are a reflection of hyperpolarization of pyramidal neurons. It is suggested that the individual components of responses evoked by direct stimulation of the cortex and thalamic nuclei have a common genesis.I. S. Beritashvili Institute of Physiology, Academy of Sciences of the Georgian SSR, Tbilisi. Translated from Neirofiziologiya, Vol. 14, No. 2, pp. 115–121, March–April, 1982.     

Role of the reticular formation of the midbrain in the process of formation of background activity of cortex neurons

In strict experimental conditions the basic activity of optic-cortex neurons in the rabbit was depressed after transverse section at the level of the rostral part of the reticular formation of the midbrain. Electrolytic destruction or functional blockage of the midbrain reticular formation (nucleus reticularis tegmenti) produces a decrease in frequency and magnitude in the grouping indexes of the cortex-neuron pulses that manifest the activity in these conditions. Destruction of specific nuclei in the optic pathway (those of the lateral geniculate body and the corpora bigemina) made no substantial change in the nature of the cortex-neuron pulses. Comparison of the parameters of pulsation activity of the same cortex neurons, as recorded before and after functional exclusion of the midbrain reticular formation, revealed that the increase in grouping of these pulses after the reticular formation was blocked induced changes in the intervals between groups of pulses, while the frequency of pulses within the groups remained constant. On the basis of the data obtained we may assume that the midbrain reticular formation plays an important role in generation of the background activity of cortex neurons, being a triggering mechanism that sets off a group of pulses. Distribution of pulses within the group is apparently due to the activity of cortex mechanisms only.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 2, No. 1, pp. 43–51, January–February, 1970.     

Influence of lithium hydroxybutyrate on the electroencephalographic effects of fenamin

Lithium hydroxybutyrate (10 mg/kg) prevents the amphetamine-induced EEG arousal and amplitude frequency alterations in the motor and visual cortex, posterior hypothalamus, midbrain reticular formation, and caudate nucleus but potentiates the action of the psychostimulant on the EEG of the hippocamp and amygdala. The response to the light flickering rhythm in the visual cortex remains within initial upon concurrent administration of both the drugs.     

Change in reactions of visual cortex neurons to light flash upon hypothalamic and reticular stimulation

The effect of electrical stimulation of the hypothalamic positive reinforcement zone (PRZ), the neutral hypothalamic zone (NZ), and the reticular formation (RF), of the midbrain on the impulse activity of single neurons of the visual cortex evoked by light flashes was studied in unanesthetized and uncurarized white rats. Poststimulus histograms of the neurons's responses were compared. Under the influence of stimulation the evoked activity of the neurons which responded to light changed in a majority of them (from 63% for the NZ to 82% for the RF). Qualitative differences were found in the effects of PRZ and RF stimulation. The effects of PRZ stimulation consisted in the invariability or in a decrease in the frequency of the neuron's discharge comprising the short-latent response, and an increase in the frequency of the discharges in the long-latent response ("positive-motivation" type). The effects of RF stimulation were manifested in intensification of the neuronal discharges in the short-latent and a decrease in the discharges in the long-latent response ("reticular" type). The responses of a considerable number of the neurons changed to the "combined" type. The data obtained indicates the different natures and mechanisms of the ascending activating effects caused by stimulation of the PRZ and the RF and which brings about positive nonspecific reinforcements. These effects, which are manifested differently in different periods of the neuron's working cycle cannot be explained by "energizing" or "tonus" concepts; their explanation must be sought for in informational (systemic) concepts.N. I. Grashchenkov Laboratory of Problems of Functional Control in the Human and Animal Organism, Academy of Sciences of the USSR, Moscow. Translated from Neirofiziologiya, Vol. 3, No. 4, pp. 359–368, July–August, 1971.     

Some mechanisms of frontal cortical participation in afferent integration

Experiments on dogs anesthetized with chloralose showed that lowering the level of activity of the frontal cortex (area F₂) by cooling leads to an increase in the negative phase of the primary response of all primary projection areas of exteroceptive stimuli (somatosensory, auditory, visual). Activation of the frontal cortex (by strychninization) increases the amplitude of the positive phase of the primary response. The same treatment applied to the frontal cortex of a cerveau isolé preparation leads to similar changes but only in the positive phase of this response. It can be concluded from the results that the frontal cortex may exert tonic effects of two types on afferent channels. One effect is indirect, through the reticular formation, by inhibition of its activating influence on the afferent channel responsible for the formation of the primary response. The other, activating effect, is aimed at the afferent channel forming the positive phase of the primary response. Under natural conditions the frontal cortex, when activated at the stage of afferent integration, evidently weakens the tone of the brain stem reticular formation and its influences, but facilitates the conduction of afferent volleys through the specific pathways, thus improving the perception of the trigger stimulus.     

Analysis of evoked potentials from the limbic cortex

In acute experiments on cats we investigated evoked potentials from the cingulate gyrus developed in response to stimulation of somatic and visceral nerves; also potentials from various parts of the hypothalamus, and midbrain reticular formation. We showed that the nonspecific afferent system influences electrical activity in the limbic cortex through hypothalamic pathways. We consider the limbic cortex to be part of the association area of the neocortex and that the associative responses of the cortex are more complex in nature than is usually thought to be the case, and that they are formed under the influence of impulses arriving at the cortex along many specific and nonspecific pathways. The hypothalamo—cingulate system is one of the main systems of cortico—subcortical integration. It plays an important part in regulation of autonomic, somatic, and emotional responses.L. A. Orbeli Institute of Physiology, Academy of Sciences of the Armenian SSR, Erevan. Translated from Neirofiziologiya, Vol. 2, No. 5, pp. 451–459, September–October, 1970.     

Modulation of the photically evoked potentials in the motor cortex of cats by influences of the somatosensory system and the reticular formation]

The influence of somatosensor stimulation and electric stimulation in the mesencephalic reticular formation upon evoked potentials (EP) of the motor cortex (MC) produced by electric stimulation of the Chiasma opticum and the Corpus geniculatum laterale, respectively, was investigated in unnarcotized, immobilized cats. By preliminary electrical stimulation of the contralateral front limb, intensity-dependent changes of the early positive component of the EP in the MC could be produced. Similar changes were found after passive movement of the limb and following electrical stimulation in the reticular formation of the mesencephalon. The EP in the MC completely disappeared after i.v. administration of 15 mg/kg Nembutal. It is concluded that the impulses reaching the MC after stimulation in the visual system can be modulated in various ways, and thus exhibit a relatively high information content. Possible mechanisms of the modulating influences and their significance are discussed.     

Effect of injury to the lateral reticular nucleus and nuclei of the inferior olive on electrical responses of the cerebellar cortex evoked by vagus nerve stimulation in cats

The role of the lateral reticular nucleus and nuclei of the inferior olive in the formation of cerebellar cortical evoked potentials in response to vagus nerve stimulation was determined in experiments on 28 cats anesthetized with chloralose and pentobarbital. After electrolytic destruction of the lateral reticular nucleus, in response to vagus nerve stimulation, especially ipsilateral, lengthening of the latent period and a decrease in amplitude of evoked potentials were observed; after bilateral destruction of this nucleus, evoked potentials could be completely suppressed. It is concluded that the lateral reticular nucleus relays interoceptive impulses in the vagus nerve system on to the cerebellar cortex. Additional evidence was given by the appearance of spike responses of Purkinje cells, in the form of mainly simple discharges, to stimulation of the vagus nerve. After destruction of the nuclei of the inferior olive, the latent period and the number of components of evoked potentials in response to vagus nerve stimulation remained unchanged but their amplitude was reduced. The role of the nuclei of the inferior olive as a regulator of the intensity of the flow of interoceptive impulses to the cerebellum is discussed.N. I. Pirogov Medical Institute, Vinnitsa. Translated from Neirofiziologiya, Vol. 9, No. 3, pp. 290–299, May–June, 1977.