精囊镜联合非那雄胺治疗慢性精囊炎反复血精的疗效分析

目的:探讨精囊镜联合非那雄胺治疗慢性精囊炎反复血精的临床有效性及安全性。方法:2012年1月至2014年8月于我院住院治疗患者中选取慢性精囊炎反复血精患者62例,应用随机数字表法,其中实验组31例,采取精囊镜联合非那雄胺治疗,对照组31例,采取单纯精囊镜手术治疗。对比两组患者有效率、复发率、症状改善及精浆果糖改善情况。结果:62例慢性精囊炎反复血精患者均顺利完成手术。实验组有效率96.77%、复发率10%,对照组有效率64.52%、复发率50%,两组比较差异有统计学意义;两组患者治疗后精浆果糖均显著升高,实验组升高程度更为明显(P0.05);实验组患者症状如会阴及下腹部不适、尿道烧灼感、性欲减退、早泄及头晕四肢乏力改善(90.32%)明显好于对照组(64.52%),差异有统计学意义。结论:精囊镜联合非那雄胺治疗慢性精囊炎反复血精安全、有效,值得临床上推广应用。     

哺乳动物精浆胞外囊泡及各组分的功能

精浆胞外囊泡是一种存在于精浆的膜性囊泡,按分泌器官分为附睾小体和前列腺小体。囊泡可与精子细胞膜发生融合,通过传递内容物或介导信号通路进而调节精子功能。它含有多种活性物质,其中蛋白质组分可影响精子活力以及顶体反应,并有清除损伤精子和促进细胞粘附的作用;脂质组分具有调节靶细胞质膜稳定性的作用;核酸组分主要参与免疫反应、跨代遗传及男性不育;离子则是多种酶的辅助因子,在调节酶活性和精浆微环境中发挥重要作用。不同组分对精子功能的影响不尽相同,本文将对此方面的研究进展进行详尽的综述,以期为该领域相关研究人员提供一定的参考。     

小鼠精囊自身抗原的原核表达和纯化

目的：在原核细胞中表达小鼠精囊自身抗原（SVA）,并对表达产物进行鉴定和纯化。方法：提取小鼠附睾组织总RNA,RT-PCR获得SVA的cDNA,设计并合成特异引物序列,进一步扩增出不含信号肽的SVA编码序列,连入原核表达载体pET28a中,经酶切和测序鉴定正确的重组质粒转化大肠杆菌Rosetta（DE3）感受态细胞,IPTG诱导表达,Western印迹分析表达产物His-SVA,采用Ni-NTA纯化融合蛋白His-SVA。结果：原核表达获得融合6个组氨酸的SVA,用抗His单克隆抗体进行Western印迹鉴定,检测到相对分子质量约18×10^3的目的蛋白,与理论值一致;经Ni-NTA纯化获得较高纯度的His-SVA融合蛋白。结论：获得了在大肠杆菌中表达的小鼠附睾蛋白SVA,为后续研究其对小鼠生殖的影响奠定了基础。     

Characterization and Partial Purification of a Novel Neuronotrophic Factor from Bovine Seminal Vesicle

Extracts from bovine seminal vesicles have been shown to contain high concentrations of nerve growth factor (NGF)-like biological activity and of the NGF protein with properties corresponding to that of NGF from other sources. We now demonstrate that a second neuronotrophic protein, termed seminal vesicle-derived neuronotrophic factor (SVNF), is present in seminal vesicle extracts (SVEs), which could not be distinguished from NGF on the basis of biological activity. SVNF has neuronotrophic activity on NGF target cells like embryonic chicken-sensory and sympathetic neurons, sympathetic neurons, and chromaffin cells from neonatal rats, but it is inactive on embryonic chicken ciliary or neonatal rat nodose ganglion neurons. It also stimulates fiber outgrowth from rat pheochromocytoma (PC 12) cells. In gel filtration chromatography on Biogel A 1.5 m, the activity is eluted with an apparent molecular weight of 40 kilodaltons, and by preparative isoelectric focusing, the isoelectric point was determined to be in the neutral range (6.8-7.8). The biological activity of SVNF, in contrast to that of NGF, is partially retained after preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and can be electrophoretically eluted with an apparent molecular weight of 16-20 kilodaltons. Electrophoretically purified SVNF is not inhibited by antisera to mouse NGF, but its activity is increased greater than 10-fold in the presence of very low concentrations of NGF. For partially purified SVNF, a specific activity of 2.9-5.8 X 10(5) biological units/mg of protein was determined in the presence of subthreshold NGF concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)     

血液流变学在临床疾病诊断、治疗中的应用

近十多年来,血液流变学在临床医学的各领域得到了广泛而深入的研究,不仅发现了许多疾病可导致血液流变学的异常,而且发现了血液流变学的变化可作为许多疾病诊断、治疗及愈后的重要指标。本文主要介绍血液流变学在这些方面的应用研究。     

Suppression of 125I-vasoactive Intestinal Polypeptide Binding Sites in Arteries of the Hamster Seminal Vesicle Following Castration

The presence and distribution of 125I-vasoactive intestinal polypeptide (VIP) binding sites in blood vessels supplying the hamster seminal vesicle was studied using a receptor autoradiographic technique before and following castration. 125I-VIP binding was studied in intact animals, in animals under a 15-day period of castration and in animals under the same period of castration but submitted to a further 15-day period of testosterone treatment.Our results show that, in the seminal vesicle, VIP-binding sites are localized in the gland smooth muscle coat and arterial smooth muscle. A 15-day castration period abolishes 125I-VIP binding to vascular smooth muscle but has no effect on 125I-VIP binding to the gland smooth muscle coat. Treatment with testosterone restores 125I-VIP binding to the vascular smooth muscle, completely reversing the effect of castration.Our results indicate that VIP-binding sites in the smooth muscle wall of arteries supplying the hamster seminal vesicle are under androgenic control and are more sensitive to androgen deprivation that VIP-binding sites associated to the gland smooth muscle coat.     

妊娠期甲状腺疾病诊断及治疗的研究进展

妊娠期妇女体内激素水平会发生变化,使妊娠妇女甲状腺激素水平的测定和判断存在一定的困难,应选择适用于妊娠妇女的甲状腺激素水平特异值,进而正确评估甲状腺功能状态及对母体和胎儿的影响。孕前及妊娠期测定促甲状腺素和游离甲状腺激素有很大的必要性,因为甲状腺疾病以及单纯性甲状腺抗体阳性会导致多种妊娠不良结局,尤其是甲状腺功能减退对胎儿智力发育和认知功能具有非常大的影响。孕期甲状腺激素的监测对评估甲状腺功能状态及疾病预后具有非常大的作用,可以提示临床医师是否给予药物干预及如何调整药量。对于孕期甲状腺激素补充治疗后应达到的目标值以及甲状腺抗体阴性的亚临床甲状腺功能降低的妊娠患者是否给予干预,目前仍有异议。     

Sperm Bundles in the Seminal Vesicle of the Crematogaster victima (Smith) Adult Males (Hymenoptera: Formicidae)

This study establishes the presence of spermatodesm in the seminal vesicles of sexually mature males of Crematogaster victima (Smith). In this species, the spermatozoa are maintained together by an extracellular matrix in which the acrosomal regions are embedded. This characteristic has not yet been observed in any other Aculeata. However, the sperm morphology in this species is similar to that described for other ants. The spermatozoa measure on average 100 μm in length, and the number of sperm per bundle is up to 256. They are composed of a head formed by the acrosome and nucleus; this is followed by the flagellum, which is formed by the centriolar adjunct, an axoneme with a 9?+?9?+?2 microtubule pattern, two mitochondrial derivatives, and two accessory bodies. The acrosome is formed by the acrosomal vesicle and perforatorium. The nucleus is filled with compact chromatin with many areas of thick and non-compacted filaments. Both mitochondrial derivatives have the same shape and diameters. The presence of sperm bundles in sexually mature males differentiates C. victima from other ants; however, the similarities in the sperm ultrastructure support the monophyly of this insect group.     

Cloning and Sequence Analysis of a cDNA from Seminal Vesicle Tissue Encoding the Precursor of the Major Protein of Bull Semen

Abstract

A cDNA library derived from poly(A)⁺RNA of bull seminal vesicle- tissue was screened with a synthetic DNA hybridisation probe specific for the major protein of bull semen. A positive clone pMP17, containing a 680 bp insert, was sequenced. In combination with primer extension sequencing of poly(A)⁺RNA, a DNA sequence of 700 bp was determined. This DNA encoded a reading frame for 134 amino acids, starting with an ATG and terminated by a TAG codon. This comprised 25 amino acids of a signal peptide followed by 109 amino acids with the known sequence of the major protein.     

Medical Diagnosis,Management and Treatment of Lesch Nyhan Disease

The aim of this presentation is to inform about Lesch Nyhan Disease from the point of view of the affected boys and their families living with the condition from day to day and also to show the importance of research in treating and managing the disease (In Caring for Children with Lesch Nyhan Disease—A Guide for Parents and Professionals; McCarthy, G.T., Ed.; PUMPA and Chailey Heritage Clinical Services: East Sussex, UK, 2002).     

血管内超声在冠状动脉疾病诊断和介入治疗中的应用

冠状动脉造影是目前公认的诊断冠状动脉病变和指导冠脉介入的金标准。然而,随着介入手术的发展,冠脉造影在评价冠脉病变方面存在的缺陷逐渐显露出来,已不能完全满足临床医生的需要。血管内超声以其优越的图像质量和空间分辨率在冠心病介入领域发挥独特的作用。作为冠脉造影的有效补充,血管内超声不仅能提供管腔和血管的直径信息,还能告知术者斑块负荷、斑块构成和血管重塑等,明确冠状动脉临界病变的性质、严重性和稳定性。此外,血管内超声还可以判断病变是否可以延迟血运重建,指导经皮冠状动脉介入的治疗策略和评估支架植入效果,有效地预防手术并发症。本文从血管内超声的概况,在冠状动脉疾病诊断和介入治疗等方面的应用进展进行了综述。