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1.
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The edible straw mushroom Volvariella volvacea is an important crop in South East Asia and is predominantly harvested in the egg stage. Rapid stipe elongation and cap expansion result in a swift transition from the egg to elongation and maturation stage, which are subjected to fast senescence and deterioration. In other mushrooms, β-1,3-glucanases have been associated with degradation (softening) of the cell wall during stipe elongation and senescence. We present a new glycoside hydrolase family 55 (GH55) exo-β-1,3-glucanase gene, exg2, and highly conserved deduced EXG2 protein. The 3D model and presumed catalytic residues of V. volvacea EXG2 are identical to Lentinula edodes EXG2 and Phanerochaete chrysosporium Lam55A, supporting similar enzymatic functions. In addition to previous association to stipe elongation and senescence, our data clearly indicates a role for cap (pileus) expansion. Digital gene expression, quantitative PCR and isobaric tags for relative and absolute quantification analysis showed low exg2 and EXG2 levels in primordia, button, egg and elongation stages and significantly increased levels in the maturation stage. Subsequent relative quantitative PCR analysis designated expression of exg2 to the stipe in the elongation stage and to the pileus and stipe in the maturation stage. EXG2 cell wall softening activity, close correlation of exg2 expression with the principal expanding mushroom tissues and a strong conservation of expression patterns and protein sequences in other mushrooms, make V. volvacea exg2 an important candidate for future studies on mechanisms of fruiting body expansion and senescence causing commodity value loss.  相似文献   

3.
Oyster mushroom (Pleurotus ostreatus) was cultivated on rice straw basal substrate, wheat straw basal substrate, cotton seed hull basal substrate, and wheat straw or rice straw supplemented with different proportions (15%, 30%, and 45% in rice straw substrate, 20%, 30%, and 40% in wheat straw substrate) of cotton seed hull to find a cost effective substrate. The effect of autoclaved sterilized and non-sterilized substrate on growth and yield of oyster mushroom was also examined. Results indicated that for both sterilized substrate and non-sterilized substrate, oyster mushroom on rice straw and wheat basal substrate have faster mycelial growth rate, comparatively poor surface mycelial density, shorter total colonization period and days from bag opening to primordia formation, lower yield and biological efficiency, lower mushroom weight, longer stipe length and smaller cap diameter than that on cotton seed hull basal substrate. The addition of cotton seed hull to rice straw and wheat straw substrate slowed spawn running, primordial development and fruit body formation. However, increasing the amount of cotton seed hull can increase the uniformity and white of mycelium, yield and biological efficiency, and increase mushroom weight, enlarge cap diameter and shorten stipe length. Compared to the sterilized substrate, the non-sterilized substrate had comparatively higher mycelial growth rate, shorter total colonization period and days from bag opening to primordia formation. However, the non-sterilized substrate did not gave significantly higher mushroom yield and biological efficiency than the sterilized substrate, but some undesirable characteristics, i.e. smaller mushroom cap diameter and relatively long stipe length.  相似文献   

4.
Stipe elongation during basidiocarp maturation in the wild-type,#5026+5132, and the elongationless mutant, NG0398, of Coprinusmacrorhizus was studied, and the following results were obtained.
  1. In the wild-type the middle zone of the stipe elongated 8.4times in 15 hr during maturation, while in the mutant it elongatedoaiy 2.2 times.
  2. Component cells of the stipe elongated inparallel with thestipe elongation in both the wild-type andthe mutant. The widthof stipe cells was almost constant duringelongation in thewild-type, while it increased 2 times in themutant. Cell volumeincreased ca. 8 times in both stocks.
  3. Theosmotic value of stipe cells was almost constant (0.45–0.50M) throughout elongation of both the wild-type and the elongationlessstipes.
  4. Mechanical properties of the cell wall were examinedby measuringshrinkage, extensibility and minimum stress-relaxationtime(To) of the stipe during maturation. These parameters weredirectlyproportional to the elongation rate to follow.
  5. Whenthe wild-type stipes were incubated in various concentrationsof mannitol solution and then in plain buffer solution, theextensibility of the stipe after the incubation in mannitolsolutions changed proportionally with the stipe length afterthe mannitol treatment, and To with the elongation capacityin plain buffer solution.
(Received March 3, 1977; )  相似文献   

5.
Stipe elongation growth is one of the remarkable characteristics of the growth and development of basidiomycete fruiting bodies. Stipe elongation is resulting from the lateral extension of stipe cells. The stipe cell is enclosed within a thin cell wall which must be loosened to expand the wall surface area for accommodation of the enlarged protoplast as the stipe cell elongates. In fungal cell walls, chitin molecules associate with each other by interchain hydrogen bonds to form chitin microfibrils which are cross-linked covalently to matrix polysaccharides. Early, some scientists proposed that stipe elongation was the result of enzymatic degradation of wall polysaccharides, whereas other researchers suggested that stipe elongation resulted from nonhydrolytic disruption of the hydrogen bonds by turgor pressure between wall polysaccharides. Recently, an extensometer was used to determine stipe wall extension for elucidation of the molecular mechanism of stipe elongation. In Coprinopsis cinerea, the native stipe cell wall is induced to extend by acidic buffers and the acid-induced native wall extension activity is located in the growing apical stipe region. A series of current experiments indicate that chitinases play a key role in the stipe wall extension, and β-glucanases mainly function in the wall remodeling for regulation of stipe wall expansibility to cooperate with chitinase to induce stipe wall extension. In addition, fungal expansin-like proteins can bind to chitin to enhance chitin hydrolysis, and their expression pattern is consistent with the stipe elongation growth, which is suggested to play an auxiliary role in the stipe wall extension.  相似文献   

6.
7.
Laboratory studies on blade growth in Alaria esculenta (L.) Grev. showed 3 periods of rapid blade elongation during the year: October–November, February–April and late June. The first two periods are characteristic of many Laminariales; the unique June peak may reflect local nutrient conditions. While the distal blade functions as a source, supplying organic matter to the blade meristem, the stipe can be a source during periods of rapid growth or a sink during late summer when blade growth is slow. Maximum enhancement of elongation rate of blade meristems was observed in 40–50 cm blades; longer blades showed no further increase in growth rate. This blade length-growth promotion relationship may be independent of seasonal variations in meristematic activity. 14C tracer experiments suggested that separate growth promotion effects by distal blade, sporophylls and stipe were not additive in the intact thallus. The preferential source of assimilate for blade meristem growth was the distal blade. Secondary sources: sporophylls, which were activated following excision of the primary source; and stipe, which began to translocate assimilate when both sources were removed. The role of secondary sources in nature is discussed. Profiles of radioactivity in alcohol-soluble organic matter in blades are evaluated in relation to tracer profiles in higher plants and mechanisms of translocation.  相似文献   

8.
姚森  刘媛媛  赵琛  严俊杰  谢宝贵 《菌物学报》2019,38(12):2232-2240
金针菇Flammulina filiformis菌柄是产量构成的重要组成部分,也是评价质量的重要指标。菌盖下方0.6-1.5cm是菌柄的伸长区,其他区段不伸长。为了研究菌柄伸长的调控机制,我们采用液相色谱-串联质谱联用(LC-MS/MS)技术对金针菇菌柄的伸长区、过渡区和非伸长区进行检测,结合层次聚类分析(HCA)、t检验等统计学方法对差异代谢物进行研究,并通过KEGG分析代谢物富集情况。结果显示,共有69种代谢物在伸长区、过渡区和非伸长区呈现梯度差异,其中52种代谢物含量为梯度性降低,17种代谢物为梯度性增加。这些差异代谢物主要归属于脂肪酸、氨基酸、核苷酸、生物碱等,其中脂肪酸及其衍生物占主导地位,尤其是油酸、花生四烯酸和肾上腺酸含量在菌柄伸长区显著高于其他区段。根据金针菇菌柄的代谢物积累特点,推测脂肪酸及其衍生物可能参与调控金针菇菌柄伸长。  相似文献   

9.
Differential elongation of stipe hyphae drives the gravitropic reorientation of Flammulina velutipes (Agaricales) fruiting bodies. The gravitropic curvature is strictly dependent on the presence of the transition zone between pileus and stipe. Elongation growth, providing the driving force for curvature, is also promoted by the pileus. Gravitropic curvature is successfully suppressed by clinostatic rotation, but the elongation rate is not affected. Explantation of fruiting body stipes lowers curvature and elongation rates corresponding to explant size reduction. In Flammulina, 25 mm length of transition zone explants is an efficient size for reproducible curvature and elongation during 48- to 72-h curvature tests. Submersion of specimens in aqueous medium causes cessation of the gravitropic curvature, but does not affect elongation. Thus the involvement of a diffusible factor in transmission of the curvature signal is probable. Splitting the fruiting body stipe in segments of 1/8 diameter does not suppress the gravitropic response, and the segments are individually reoriented to the vertical. It is concluded that the graviresponse of the Flammulina fruiting body is based on cellular perception of the gravistimulus and that a differential growth signal is transmitted in the stipe by a soluble factor that regulates hyphal elongation.  相似文献   

10.
金针菇具有很高的营养与保健价值,菌柄长短决定金针菇的产量与品质,而菌柄伸长的相关作用酶及分子机理尚不清楚。前期草菇中发现外切-β-1,3-葡聚糖酶基因(exg2)可能与菌柄伸长相关,但在金针菇中尚没有exg基因的相关报道。本研究首先在金针菇全基因组中鉴定到3个外切-β-1,3-葡聚糖酶家族基因(分别命名为:Ffexg1Ffexg2Ffexg3),并进行了克隆验证。进一步采用定量PCR对3个基因在金针菇不同发育时期及组织部位的差异性表达进行了分析。结果显示:Ffexg1只在菌柄中高表达,Ffexg2Ffexg3在菌柄中表达量先上升后下降,在菌盖中呈逐渐上升趋势。3个Ffexg基因均在菌柄发生伸长的部位表达量较高,且在菇体水平放置后菌柄弯曲程度较大的部位表达量较高。结果显示金针菇exg家族3个基因存在时空差异性表达,在菌柄中伸长较快的时期及部位伴随着Ffexg基因的高表达。结合其功能预测,Ffexg家族基因可能作用于细胞壁成分β-1,3-葡聚糖链,从而在金针菇菌柄及菌盖发育中起作用。  相似文献   

11.
Background and Aims Plant stature and shape are largely determined by cell elongation, a process that is strongly controlled at the level of the cell wall. This is associated with the presence of many cell wall proteins implicated in the elongation process. Several proteins and enzyme families have been suggested to be involved in the controlled weakening of the cell wall, and these include xyloglucan endotransglucosylases/hydrolases (XTHs), yieldins, lipid transfer proteins and expansins. Although expansins have been the subject of much research, the role and involvement of expansin-like genes/proteins remain mostly unclear. This study investigates the expression and function of AtEXLA2 (At4g38400), a member of the expansin-like A (EXLA) family in arabidposis, and considers its possible role in cell wall metabolism and growth.Methods Transgenic plants of Arabidopsis thaliana were grown, and lines over-expressing AtEXLA2 were identified. Plants were grown in the dark, on media containing growth hormones or precursors, or were gravistimulated. Hypocotyls were studied using transmission electron microscopy and extensiometry. Histochemical GUS (β-glucuronidase) stainings were performed.Key Results AtEXLA2 is one of the three EXLA members in arabidopsis. The protein lacks the typical domain responsible for expansin activity, but contains a presumed cellulose-interacting domain. Using promoter::GUS lines, the expression of AtEXLA2 was seen in germinating seedlings, hypocotyls, lateral root cap cells, columella cells and the central cylinder basally to the elongation zone of the root, and during different stages of lateral root development. Furthermore, promoter activity was detected in petioles, veins of leaves and filaments, and also in the peduncle of the flowers and in a zone just beneath the papillae. Over-expression of AtEXLA2 resulted in an increase of >10 % in the length of dark-grown hypocotyls and in slightly thicker walls in non-rapidly elongating etiolated hypocotyl cells. Biomechanical analysis by creep tests showed that AtEXLA2 over-expression may decrease the wall strength in arabidopsis hypocotyls.Conclusions It is concluded that AtEXLA2 may function as a positive regulator of cell elongation in the dark-grown hypocotyl of arabidopsis by possible interference with cellulose metabolism, deposition or its organization.  相似文献   

12.
Expansin proteins extend plant cell walls by a hydrolysis-free process that disrupts hydrogen bonding between cell wall polysaccharides. However, it is unknown if this mechanism is operative in mushrooms. Herein we report that the native wall extension activity was located exclusively in the 10 mm apical region of 30 mm Flammulina velutipes stipes. The elongation growth was restricted also to the 9 mm apical region of the stipes where the elongation growth of the 1st millimetre was 40-fold greater than that of the 5th millimetre. Therefore, the wall extension activity represents elongation growth of the stipe. The low concentration of expansin-like protein in F. velutipes stipes prevented its isolation. However, we purified an expansin-like protein from snail stomach juice which reconstituted heat-inactivated stipe wall extension without hydrolytic activity. So the previous hypotheses that stipe wall extension was resulted from hydrolysis of wall polymers by enzymes or disruption of hydrogen bonding of wall polymers exclusively by turgor pressure are challenged. We suggest that stipe wall extension may be mediated by endogenous expansin-like proteins that facilitate cell wall polymer slippage by disrupting noncovalent bonding between glucan chains or chitin chains.  相似文献   

13.
A combination of quantitative water density and T2 MRI and changes therein observed after infiltration with 'invisible' Gd-DTPA solution was used to study cell water balances, cell water potentials and cell integrity. This method was applied to reveal the evolution and mechanism of redistribution of water in harvested mushrooms. Even when mushrooms did not lose water during the storage period, a redistribution of water was observed from stipe to cap and gills. When the storage condition resulted in a net loss of water, the stipe lost more water than the cap. The water density in the gill increased, probably due to development of spores. Deterioration effects (i.e. leakage of cells, decrease in osmotic water potential) were found in the outer stipe. They were not found in the cap, even at prolonged storage at 293 K and R.H.=70%. The changes in osmotic potential were partly accounted for by changes in the mannitol concentration. Changes in membrane permeability were also indicated. Cells in the cap had a constant low membrane (water) permeability. They developed a decreasing osmotic potential (more negative), whereas the osmotic potential in the outer stipe increased, together with the permeability of cells.  相似文献   

14.
The fruiting ofFavolus arcularius in culture is described. When the cultures, which have been pre-incubated in darkness to allow the inoculum mycelia to become thick and white wooly in texture, are exposed to light, fruit-body primordia, 1 mm in height, are formed about 4 days after the start of illumination. The primordium develops into a cylindrical stipe, the growth of which mainly occurs in the final 1 mm of the terminal region. Hyphal elongation in the region within 3 mm of the apex is predominant in the growth of the pileate stipe. With maturation of the stipe, changes in hyphal orientation occur on the periphery of the subapical region, and then the pileus-primordium is formed. The differentiation into the inner layer and the outer layer (pre-hymenial layer) in the pileus tissue is completed at this stage. The early growth of the pileus may be due to rapid elongation of the hyphae on the margin in addition to gradual expansion of the hyphae in the preformed pseudo-tissue. When the pileus has grown to about 3 mm in diameter, the subsequent three to four fold increase in size may be due to parallel expansion of the hyphae constituting the young pileus tissue.  相似文献   

15.
In the growing apex of Arabidopsis thaliana primary roots, cells proceed through four distinct phases of cellular activities. These zones and their boundaries can be well defined based on their characteristic cellular activities. The meristematic zone comprises, and is limited to, all cells that undergo mitotic divisions. Detailed in vivo analysis of transgenic lines reveals that, in the Columbia-0 ecotype, the meristem stretches up to 200 µm away from the junction between root and root cap (RCJ). In the transition zone, 200 to about 520 µm away from the RCJ, cells undergo physiological changes as they prepare for their fast elongation. Upon entering the transition zone, they progressively develop a central vacuole, polarize the cytoskeleton and remodel their cell walls. Cells grow slowly during this transition: it takes ten hours to triplicate cell length from 8.5 to about 35 µm in the trichoblast cell files. In the fast elongation zone, which covers the zone from 520 to about 850 µm from the RCJ, cell length quadruplicates to about 140 µm in only two hours. This is accompanied by drastic and specific cell wall alterations. Finally, root hairs fully develop in the growth terminating zone, where root cells undergo a minor elongation to reach their mature lengths.Key words: Arabidopsis, cytoskeleton, development, differentiation zone, elongation zone, growth, growth terminating zone, meristem, root apex, transition zone  相似文献   

16.
We characterized vegetative development in two inbred cell lines of Acetabularia acetabulum (L.) Silva. Cell growth occurred at the apex and by elongation of older interwhorls throughout vegetative development. Although cell length and hairs per whorl increased regularly during development, interwhorl length, hair persistence on the stalk, and complexity of each whorl (degree of branching of whorl hairs) showed sharp discontinuities during development in both cell lines. The first (earliest) discontinuity, formation of a short interwhorl, was the sixth interwhorl made in all cells. Even though cell line Aa1055 was twice the height ofAa4010 when mature, cells in both lines were 0.8–1.0 cm tall after formation of the short interwhorl. The second discontinuity, increases in hair persistence on the stalk and complexity of each whorl of hairs, began shortly before cap initiation. We propose the following nomenclature: 1) that slower growth before formation of the short interwhorl be called “juvenile”; 2) that more rapid growth after formation of the short interwhorl be called “adult”; and 3) that adult growth be separated into “early” and “late” phases by the discontinuities in whorl hair characteristics. The proposed developmental phases (juvenile, early adult, and late adult) are temporally sequential and spatially stacked.  相似文献   

17.
We show that fruit bodies of Flammulina velutipes can be induced in complete darkness after a sharp temperature reduction (23° to 16°C). However, the fruit bodies that form in complete darkness have a long stipe with an undeveloped pileus on the top (pinhead fruit bodies) and are thinner and whiter than the normal fruit bodies which are formed in the light. This finding suggests that F. velutipes fruit bodies cannot mature in complete darkness. However, when we irradiated the fruit bodies that had formed in complete darkness, a pileus developed immediately, and 4 days later the separation between the stipe and the pileus could be observed. Immediately after light exposure, the stipe also thickened and became increasingly pigmented. The stipe elongation was inhibited until 8 days after light exposure, although stipe elongation progressed very quickly thereafter. Basidospores were also visible in the gills 8 days after light exposure. We consider that the basidiospore development is involved in this rapid stipe elongation, which aids the effective dispersal of basidiospores.  相似文献   

18.
The fruiting body of the basidiomycete Flammulina velutipes shows a distinct negative gravitropic response. Maturing fruiting bodies in the rapid elongation phase become graviresponsive with basidiospore differentiation. Lateral gravistimulation by horizontal arrangement of the fruiting body results in unilateral growth regulation. Elongation in the upper Stipe side decreases to 40% during gravitropic reorientation of the fruiting body. Overshooting of the gravitropic response during reorientation is precisely regulated. The graviresponsiveness is concentrated to the apical area of the stipe, the transition zone between pileus and stipe, which features a prominent elongation capability. The small size and low vacuolization of the transition zone hyphae compared with differentiated basal stipe hyphae correspond with this physiological function on the light and electron microscopical levels. Curvature experiments using intact and explanted fruiting bodies demonstrated the graviperceptive role of the transition zone. The excision of various amounts of pilear tissue, even the disruption of the whole pileus, had no severe effect on gravitropic curvature, until the transition zone was damaged. Removal of the transition zone resulted in a dramatic loss of graviresponse, whereas the decrease of elongation was less drastic.  相似文献   

19.
Preliminary evidence is presented which indicates that far-red irradiation promotes and near-red inhibits rapid stipe elongation in Nereocystis luetkeana (Mertens) Postels & Ruprecht. Laboratory results showed afar-red reversal of red inhibition, but a red/far-red reversal was not evident in field experiments. The effects of changing ratios of red and far-red irradiation brought about by selective water absorption are discussed in relation to stipe elongation of Nereocystis.  相似文献   

20.
Pea epicotyls (Pisum sativum, cv. Alaska) were enclosed in chambers in which their elongation was restricted by means of a foam neoprene stopper or by a medium of glass beads. These treatments increased evolution of ethylene and resulted in reduced length and increased diameter of both the internodes and the cells of the internodes. These responses increased with increasing degrees of restriction. A time-sequence study of the emergence of epicotyls through 90 mm of glass beads showed that an accelerated evolution of ethylene preceded a reduction in elongation. As the epicotyls elongated through the glass bead medium and less resistance was encountered, evolution of ethylene declined and rapid elongation was resumed. The morphological and anatomical effects of a 120-mm column of glass beads were duplicated by applied ethylene concentrations of 0.2 ppm or less. Evolution of CO2 was inhibited slightly by the ethylene treatments. The data indicate that production of ethylene by pea epicotyls is increased by nonwounding physical stress, and that the ethylene acts as an endogenous growth regulator, decreasing elongation and increasing diameter in response to increasing increments of stress.  相似文献   

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