共查询到20条相似文献,搜索用时 31 毫秒
1.
R Corrocher S Ferrari A Bassi P Guarini L Bertinato O Olivieri G M Licia O Ruzzenente C Brugnara G De Sandre 《Life sciences》1987,41(9):1171-1178
Two groups of individuals, 26 normotensive normolipemic and 37 normotensive hyperlipemic, all without family history of hypertension have been selected in attempt to demonstrate whether Li-Na countertransport of erythrocytes is influenced by plasma and membrane lipid composition. The maximal rate of Li-Na countertransport was elevated in hyperlipemics (0.344 +/- 0.168 vs 0.220 +/- 0.074 mmol/l erythrocytes/h). This difference is highly significant. Hyperlipemics had different composition of membrane lipids than normals. The most important variations were: increase of palmitic, palmitoleic and total saturated fatty acids (SFA) as well as increase of cholesterol/phospholipids ratio (C/PL); in contrast, hyperlipemics had a reduced amount of linoleic acid and total unsaturated fatty acids (UFA) as well as total polyunsaturated fatty acids (PUFA). Consequently, UFA/SFA and PUFA/SFA ratios were lower than in normals. Li-Na countertransport was negatively correlated with the amount of PUFA (P less than 0.02), whereas it was positively correlated with the following parameters: oleic/linoleic ratio (p less than 0.02), monounsaturated fatty acids/polyunsaturated fatty acids ratio (p less than 0.03) as well as with the SFA + monounsaturated fatty acid/PUFA ratio (p less than 0.03). These findings suggest that the V max of Li-Na countertransport in erythrocytes is influenced by the lipid composition of the membrane. 相似文献
2.
Spatial patterns of seston concentration and biochemical composition between nearshore and offshore waters of a Great Lake 总被引:1,自引:0,他引:1
RALPH E. H. SMITH CHRISTOPHER C. PARRISH DAVID C. DEPEW ANAS GHADOUANI 《Freshwater Biology》2007,52(11):2196-2210
1. The concentration and composition of seston were measured in the east basin of Lake Erie (U.S.A.–Canada) to determine whether the classical patterns of nearshore versus offshore zonation considered typical of large lakes have been altered by decades of change in nutrient loading and food web structure, and to test the possibility that food quality for seston consumers, as reflected in nutrient and lipid composition, may currently be diminished. Chlorophyll‐a (chl‐a), particulate organic carbon (POC), particulate P, total lipid (TL), lipid classes, fatty acids and major phytoplankton groups were compared between the nearshore (<20 m) and offshore zones in spring and early summer. 2. Particulate organic carbon, chl‐a and TL concentrations were lower nearshore than offshore, which is a reversal of the classical large lake pattern but consistent with other evidence from the Great Lakes that colonization by filter‐feeding dreissenid mussels can cause seston depletion in relatively shallow waters. 3. Terrestrial and bacterial fatty acid markers were slightly higher in the nearshore than the offshore, but there was little difference in nutrient, taxonomic or biochemical composition between zones otherwise. This shows a small differential influence of terrestrial and detrital inputs, and a slight shift away from phytoplankton dominance of seston composition in the nearshore, but little difference in potential food quality of seston. 4. Microscope counts, phytoplankton fluorescence, and fatty acid composition indicated succession from diatom dominance in May to dominance by cryptophyte and chromophyte flagellates in June and July. Depletion of dissolved P and Si, increased contribution of storage lipids, and changes in fatty acid composition suggested a mild degree of nutrient deficiency during this spring to summer transition. 5. Total lipids were dominated by classes typical of pigments and membranes with only moderate amounts of neutral (storage) lipids, polyunsaturated fatty acids (PUFA) were abundant (especially the long chain ω‐3 acids) and the POC : chl‐a and POC : particulate P ratios indicated P sufficiency or slight P‐deficiency. Despite concerns that some important consumers in the Great Lakes food web may be suffering from diminished food quality, these results point to a generally high seston food quality even with highly abundant mussels and incipient P deficiency in the east basin of Lake Erie. 相似文献
3.
Because of its local commercial value, the proximate nutritional composition, fatty acid composition, cholesterol and fat‐soluble vitamins of spiny eel (Mastacembelus simack Walbaum, 1792) were determined in 20 specimens caught in Keban Dam Lake, Elaz??, Turkey in September 2009. Moisture, protein, lipid, ash and cholesterol contents of the spiny eel were 75.9, 17.1, 10.9, 1.0% and 52.6 mg per 100 g, respectively. Fatty acid composition showed that total polyunsaturated fatty acids (PUFA) were the highest (37.1%), followed by monounsaturated fatty acids (MUFA, 35.5%) and saturated fatty acids (SFA, 27.4%). The relation between PUFA and SFA (1.35) was comparable to that of some fish species, and PUFA : MUFA : SFA ratio (1.35 : 1.29 : 1) was very close to that recommended by nutritionists. Among the vitamins (A, D, E and K) analyzed, the vitamin E content was highest followed by D, K and A. In conclusion, M. simack is rich in proteins, unsaturated fatty acids and vitamins and low in cholesterol, with a reasonable PUFA : SFA and PUFA : MUFA : SFA ratio as recommended by nutritionists. M. simack can therefore be recommended for human consumption as a good source of nutrition. 相似文献
4.
Juhel C Senft M Pafumi Y Girard-Globa A Levy E Lafont H Lairon D 《The Journal of nutritional biochemistry》1999,10(8):458-466
Although several investigations have linked the degree of fatty acid saturation to plasma lipid responses in the postprandial state, further evaluation is necessary. In this study, we compared the effect of saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids on postprandial lipid metabolism using complementary in vivo and in vitro approaches. Fat (10 g) cholesterol (0.5 g) test meals that provided either lard (SFA), olive oil (MUFA), or sunflower oil (PUFA) were ingested by chow-fed New Zealand white rabbits (n = 8). In addition, hepatic uptake of triglyceride-cholesterol-rich lipoproteins (TCRL) isolated from rabbits chronically ingesting SFA, MUFA, or PUFA diets was measured using freshly isolated chow-fed rabbit hepatocytes. Whatever dietary fatty acids ingested, postprandial triglyceridemia and occurrence of radiolabelled dietary lipids in plasma were not markedly different. Conversely, SFA induced higher postprandial cholesterolemia and phospholipemia than MUFA (P < 0.05) whereas PUFA prevented postprandial cholesterol increase. TCRL disappearance from cultured liver cell media was delayed with SFA-rich TCRL and faster with PUFA whereas MUFA-rich TCRL showed an intermediate figure. From these data, we conclude that SFA, MUFA, and PUFA elicited different postprandial plasma and lipoprotein lipid responses. The fatty acid composition of TCRL had a major impact on their subsequent metabolism, especially uptake by cultured hepatocytes. The SFA-induced hypercholesterolemia could be related to an altered hepatic uptake whereas a faster clearance and hepatic uptake could explain the cholesterol-lowering effect of PUFA in rabbits. MUFA, like PUFA, accelerate uptake by hepatocytes but favor cholesterol ester enrichment of TCRL. 相似文献
5.
Guillermo E. Napolitano Ricardo J. Pollero Ana M. Gayoso Bruce A. Macdonald Raymond J. Thompson 《Biochemical Systematics and Ecology》1997,25(8):739-755
The fatty acid compositions of phytoplankton and major primary consumers were analyzed during the development of seasonal algal blooms in the Bahía Blanca estuary, situated on the southern coast of the province of Buenos Aires (Argentina), and Trinity Bay, at Sunnyside, on the eastern coast of Newfoundland (Canada). Primary consumers in the Bahía Blanca estuary were zooplankton dominated by the calanoid copepod Acartia tonsa. At Sunnyside, the primary consumers were the sea scallop Placopecten magellanicus, an ecological and economical important benthic bivalve. The study shows that in spite of obvious differences between the two environments and the analytical approaches employed in each case, the analyses of fatty acid biomarkers can provide relevant ecological information. The fatty acid composition of the lipids of Bahía Blanca phytoplankton (high concentrations of the fatty acids 14:0, 16:4ω1, and 20:5ω3) reflected the presence of diatoms as a major component throughout the bloom. Fatty acid markers of the post-bloom phytoplankton in Bahía Blanca indicated a decline of phytoplankton biomass, and a relatively high input of detritus and terrestrial plant materials to the particulate organic matter of the estuary. Linoleic acid (18:2ω6), a typical “terrestrial” fatty acid, was conspicuous in the lipids of the post-bloom particulate matter of the Bahía Blanca estuary; 18:2ω2 was subsequently incorporated into zooplankton lipids diatom markers were also prominent in the lipids of pre-bloom and bloom phytoplankton at Sunnyside; post-bloom phytoplankton showed higher proportions of 18:0, 18:1ω9, and 18:4ω3, characteristic and often major fatty acids of dinoflagellates. The fatty acids of the digestive gland of P. magellanicus reflected the fatty acid composition of the phytoplankton, whereas those of the adductor muscle were practically unaffected by the composition of the food. This organ-specific response of an animal to the fatty acid composition of the diet is examined in terms of different applications of the fatty acid marker concept. 相似文献
6.
Luz Fernandez M West KL Roy S Ramjiganesh T 《The Journal of nutritional biochemistry》2001,12(12):703-710
Male, female and ovariectomized (to mimic menopause) guinea pigs were fed a saturated (SFA) or a polyunsaturated (PUFA) fat diet for 4 weeks to determine the effects of dietary fat saturation on lipoprotein levels and composition and to assess whether gender and hormonal status modulate the cholesterolemic response. Both diets contained 15g/100 g fat and 0.04 g/100 g cholesterol and were identical in composition except for the type of fat. The SFA diet contained 50% saturated fat (25% lauric + myristic fatty acids), 25% PUFA and 25% monounsaturated fatty acids while the PUFA diet had 50% PUFA (linoleic acid), 25% monounsaturated and 25% SFA fatty acids. Plasma LDL cholesterol (LDL-C) was an average 21% lower in guinea pigs fed PUFA compared to those fed SFA (P < 0.05). In addition, ovariectomized guinea pigs, both in the SFA and PUFA groups, had 20–33% higher LDL-C than either males or females (P < 0.01). VLDL cholesterol was 70% higher in the PUFA-fed animals (P < 0.0001). A gender effect was observed in plasma HDL cholesterol (HDL-C) with females and ovariectomized guinea pigs having 30–42% higher HDL-C than males (P < 0.01). LDL susceptibility to oxidation was not affected by dietary fat saturation or gender. In contrast, VLDL and LDL composition were significantly influenced by diet and gender. VLDL particles were larger in size in guinea pigs fed the SFA diets (P < 0.01) while LDL particles were larger in female guinea pigs (P < 0.001). Hepatic lipids were influenced by the interaction between diet and group. Hepatic cholesterol (P < 0.01) and TAG concentrations (P < 0.0001) were highest in female guinea pigs fed the PUFA diet. Since the liver is the major site of lipoprotein synthesis and catabolism, these results suggest that not only diet but also gender may play a major role in determining the composition and size of lipoproteins. 相似文献
7.
《Animal : an international journal of animal bioscience》2015,9(10):1721-1730
Quality of pork depends on genotype, rearing and pre- and post-slaughter conditions. However, no information is available on rearing system changes and oleic acid supplementation on carcass characteristics and fatty acid (FA) profile of pork from the Alentejano (AL) pig, an obese breed. This study evaluates the effects of feeding low (LO) or high oleic acid diets (HO) to AL pigs reared in individual pens (IND) or outdoor (OUT) with access to pasture. Carcass composition was obtained and longissimus dorsi and semimembranosus samples were collected to analyse chemical composition and neutral and polar intramuscular lipids FA profile by gas chromatography. Statistical analysis was performed by a two-way ANOVA for rearing system and diet effects. OUT-reared pigs presented leaner carcasses than IND-reared ones. Both muscles presented lower intramuscular lipid content in OUT-reared pigs. Treatments affected the FA profile of muscles. Overall, OUT-reared pigs presented lower n-6/n-3 FA ratios, whereas pigs fed the HO diet exhibited lower saturated fatty acids (SFA), higher monounsaturated fatty acids (MUFA) levels and lower thrombogenic indexes on neutral intramuscular lipids than LO-fed pigs. On the polar fraction, OUT-reared pigs presented lower SAT and n-6/n-3 FA ratio, and higher polyunsaturated fatty acids (PUFA) levels on both muscles. Pigs fed the HO diet exhibited higher MUFA and lower PUFA levels on both muscles, and lower SAT levels on semimembranosus. This study shows rearing system and oleic acid supplementation have complementary effects and influence carcass composition and the nutritional quality of meat. 相似文献
8.
M. A. Sahari F. Farahani Y. Soleimanian A. Javadi 《Zeitschrift fur angewandte Ichthyologie》2014,30(2):381-391
In the current study, the effect of frozen storage at ?18°C was evaluated on fatty acid composition of different body parts (liver, muscle tissue, and viscera) of narrow‐barred Spanish mackerel (Scomberomorus commerson, Lacépède, 1800), longtail tuna (Thunnus tonggol, Bleeker, 1851), kawakawa (Euthynnus affinis, Cantor, 1849), king mackerel (Scomberomorus guttatus, Bloch & Schneider, 1801), and rainbow sardine (Dussumieria acuta, Valenciennes, 1847) caught in the Persian Gulf. Changes in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid plus docosahexaenoic acid/palmitic acid (EPA+DHA/C16), ω3 PUFA/ω6 PUFA (ω3/ω6), and polyunsaturated fatty acids/saturated fatty acids (PUFA/SFA) were investigated during a 6‐month period. A decrease in unsaturated fatty acids, particularly PUFAs (60–100%) as well as ω3/ω6, EPA+DHA/C16 (polyene index) and PUFA/SFA ratios, indicated a decrease in the nutritional values of the samples. 相似文献
9.
Adrie Ykema Elizabeth C. Verbree Ira I. G. S. Verwoert Karin H. van der Linden H. John J. Nijkamp Henk Smit 《Applied microbiology and biotechnology》1990,33(2):176-182
Summary From six unsaturated fatty acid auxotrophs (Ufa mutants) of the oleaginous yeast Apiotrichum curvatum blocked in the conversion of stearic to oleic acid, were isolated revertants able to grow in the absence of unsaturated fatty acids, in a search for strains that can produce cocoa butter equivalents. A broad range in the percentage of saturated fatty acids (%SFA) was observed in the lipids of individual revertants (varying from 27%–86% SFA), compared with the wild-type (44% SFA). Further analysis of fatty acid composition indicated that: (i) not all six Ufa mutants had the same genetic background and (ii) one specific Ufa mutation could be reverted in more than one way. Revertants that produced lipids with a %SFA>56%, were examined further. These strains were cultivated for 50 generations and half of them produced lipids with high %SFA after that time and were defined as stable. The viability of revertant strains with extremely high %SFA (>80%) may be explained by our finding that polar lipids, which are part of yeast membranes, contained much more polyunsaturated fatty acids and a significantly lower %SFA than neutral (storage) lipids. One revertant (R25.75) was selected that was able to produce lipids in whey permeate at a rate comparable with wild-type A. curvatum and with a fatty acid composition and congelation curve comparable with cocoa butter.
Offprint requests to: A. Ykema 相似文献
10.
Influence of zooplankton and phytoplankton on the fatty acid composition of digesta and tissue lipids of silver carp: mesocosm experiment 总被引:1,自引:0,他引:1
Zooplankton appeared to be the major contributor to the diet of 1+ silver carp, whereas 3+ fishes exhibited a more evenly balanced spectrum between zooplankton and phytoplankton. The fatty acids profiles of digesta were influenced by zooplankton, particularly for 1+ silver carp. Together, fatty acid profiles of tank zooplankton and digesta were characterized by high proportion of 20 : 5ω3 and 20 : 6ω3. The fatty acids composition of the phytoplankton reflected the dominance of cyanobacteria and chlorophycea, with high quantities of 18 : 2ω6 and 18 : 3ω3. Although cyanobacteria accounted for >70% of the phytoplankton biomass ingested by the carp, fatty acids profiles of digesta were not influenced by phytoplankton fatty acids composition. The low digestive and conversion efficiency of Microcystis aeruginosa explain this absence of relation. The neutral lipids in silver carp tissues reflected poorly the fatty acids profiles in the diet, the semi-natural conditions and the diet dominated throughout the study by zooplankton, led to little variation in tissues fatty acids. The phospholipids in the muscle, liver and peri-intestinal fat were characterized by a rather low proportion of polyunsaturated fatty acids (PUFA) in both 1+ and 3+ fish. From a qualitative view point, cryptophycea, diatoms, and especially zooplankton are much more valuable food for the silver carp than cyanobacteria and desmid chlorophycea which are poor in long-chain PUFA. 相似文献
11.
Hiroaki Saito Changhu Xue Rieko Yamashiro Satoshi Moromizato Yutaka Itabashi 《Journal of phycology》2010,46(4):665-673
The lipid and fatty acid compositions in two edible subtropical algae (the brown alga Cladosiphon okamuranus Tokida and the green alga Caulerpa lentillifera J. Agardh) were determined to clarify their lipid characteristics and nutritional values. Glycolipids and phospholipids were the major lipid classes, with significant levels of triacylglycerols. Polyunsaturated fatty acids (PUFA) were the major fatty acids of both algae. The lipid class composition and major fatty acids were similar in both the algal species, irrespective of wild and cultured specimens. Typical n‐6 PUFA, such as 18:2n‐6 (linoleic acid) and 20:4n‐6 (arachidonic acid), occurred in characteristically high levels in both of the algae. High levels of n‐3 PUFA were measured in all lipid classes of both species without 22:6n‐3 (docosahexaenoic acid), 18:3n‐3, 18:4n‐3, and 20:5n‐3 (eicosapentaenoic acid) for Cl. okamuranus; and 16:3n‐3, 18:3n‐3, and 20:5n‐3 for Ca. lentillifera. The finding suggests that the green algal species, which mainly biosynthesizes short‐chain (C16 and C18) PUFA, differs from that of the brown alga, which is capable of biosynthesizing high 20:5n‐3 levels. The PUFA levels in glycolipids of the two algal species comprised up to 60%, even though they are subtropical marine species. High n‐6 PUFA levels in the algal lipids probably influence the significant levels of n‐6 PUFA in herbivorous fishes, because the n‐6 PUFA levels in marine fish lipids are generally undetectable or negligible. 相似文献
12.
Meryem Yeşim Çelik Mehmet Bedrettin Duman Merve Sariipek Gülşen Uzun Gören Dilara Kaya Öztürk 《Molluscan research.》2019,39(3):234-240
ABSTRACTThe fatty acid and mineral matter compositions of the visceral mass and pedal mass of wild and farmed Cornu aspersum were compared. The relative fatty acid amounts of the visceral and pedal masses of wild and farmed snails are: polyunsaturated fatty acid (PUFA) > saturated fatty acid (SFA) > monounsaturated fatty acid (MUFA), PUFA?>?SFA?>?MUFA, MUFA?>?PUFA?>?SFA and PUFA?>?MUFA?>?SFA, respectively. Wild snails had a higher concentration of iron and zinc than farmed snails. Selenium was 2.82 times higher in the pedal mass of farmed snails than the pedal mass of wild snails. Ca:P ratios in the visceral mass of wild snails, visceral mass and pedal mass of farmed snails were 1.76:1, 1.12:1 and 3.87:1 respectively. Both wild and farmed snails have high contents of oleic, linoleic, eicosapentaenoic and docosahexaenoic fatty acids and are a good source of minerals. Careful attention should be paid, however, as to which diet will be used during farming because the composition of fatty acid in the snail is affected by diet. 相似文献
13.
G. Maulucci O. Cohen B. Daniel A. Sansone P. I. Petropoulou S. Filou 《Free radical research》2016,50(10):S40-S50
AbstractMetabolic homeostasis of fatty acids is complex and well-regulated in all organisms. The biosynthesis of saturated fatty acids (SFA) in mammals provides substrates for β-oxidation and ATP production. Monounsaturated fatty acids (MUFA) are products of desaturases that introduce a methylene group in cis geometry in SFA. Polyunsaturated fatty acids (n-6 and n-3 PUFA) are products of elongation and desaturation of the essential linoleic acid and α-linolenic acid, respectively. The liver processes dietary fatty acids and exports them in lipoproteins for distribution and storage in peripheral tissues. The three types of fatty acids are integrated in membrane phospholipids and determine their biophysical properties and functions. This study was aimed at investigating effects of fatty acids on membrane biophysical properties under varying nutritional and pathological conditions, by integrating lipidomic analysis of membrane phospholipids with functional two-photon microscopy (fTPM) of cellular membranes. This approach was applied to two case studies: first, pancreatic beta-cells, to investigate hormetic and detrimental effects of lipids. Second, red blood cells extracted from a genetic mouse model defective in lipoproteins, to understand the role of lipids in hepatic diseases and metabolic syndrome and their effect on circulating cells. 相似文献
14.
Indurain G Beriain MJ Sarries MV Insausti K 《Animal : an international journal of animal bioscience》2010,4(10):1771-1780
The effect of slaughter weight and breed on the composition of intramuscular fat (IMF) of seven Spanish cattle breeds was studied. Lipid classes and fatty acids were evaluated in young bulls of seven local Spanish cattle breeds, Asturiana, Avileña, Morucha, Parda Alpina, Pirenaica, Retinta and Rubia Gallega, slaughtered at a live weight of 320 kg (veal type) and 550 kg (yearling bull type). Higher slaughter weight and early maturating breeds produced higher IMF content in the longissimus muscle (P < 0.001), which was linked to a reduction in phospholipids, monoglycerides, diglycerides, cholesterol and free-fatty acids content, but an increase in triacylglycerols. Besides, heavier animals displayed a higher percentage of saturated and monounsaturated fatty acids (MUFA), but a lower polyunsaturated fatty acids (PUFA) percentage (P < 0.001). The increase in triglycerides (TG), saturated fatty acids (SFA) and MUFA illustrates the increasing importance of lipid storage as fattening proceeds and the acquisition of ruminal functionality. The feeding system based on cereals had a strong influence on the fatty acid profile, giving the studied beef a relatively high PUFA/SFA and n-6/n-3 ratios. 相似文献
15.
F. Geiser R. P. Learmonth 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1994,164(1):55-61
The composition of tissue and membrane fatty acids in ectothermic vertebrates is influenced by both temperature acclimation and diets. If such change in body lipid composition and thermal physiology were linked, a diet-induced change in body lipid composition should result in a change in thermal physiology. We therefore investigated whether the selected body temperature of the agamid lizardAmphibolurus nuchalis (body mass 20 g) is influenced by the lipid composition of dietary fatty acids and whether diet-induced changes in thermal physiology are correlated with changes in body lipid composition. The selected body temperature in two groups of lizards was indistinguishable before dietary treatments. The selected body temperature in lizards after 3 weeks on a diet rich in saturated fatty acids rose by 2.1 °C (photophase) and 3.3 °C (scotophase), whereas the body temperature of lizards on a diet rich in unsaturated fatty acids fell by 1.5 °C (photophase) and 2.0 °C (scotophase). Significant diet-induced differences were observed in the fatty acid composition of depot fat, liver and muscle. These observations suggest that dietary lipids may influence selection of body temperature in ectotherms via alterations of body lipid composition.Abbreviations bm
body mass
- FA
fatty acid(s)
- MUFA
monounsaturated fatty acids
- PUFA
polyunsaturated fatty acids
- SFA
saturated fatty acids
-
T
a
air temperature
-
T
b
body temperature
- UFA
unsaturated fatty acids 相似文献
16.
Sperm plasma membrane lipids have an important role to play in determining membrane fluidity and sperm motility. The objective of the present study was to determine whether there are differences in the lipid and fatty acid (FA) composition of boar sperm and seminal plasma in the ejaculates of boars having different sperm motilities. Semen was collected from two groups of boars having normal (> 60%; n = 53) or low (< 60%; n = 53) motility sperm and the semen was evaluated for motility, morphology and vitality. The semen was then centrifuged to separate the sperm from the seminal plasma and both were kept at −20 °C until analyzed for lipid content and FA profile by gas chromatography. Total antioxidant status (TAS) of seminal plasma was determined using a commercial kit. There were differences (P ≤ 0.05) in sperm total lipids, cholesterol, saturated fatty acids (SFA), phospholipids, n-3 polyunsaturated fatty acids (PUFA), docosahexaenoic acid (DHA) and the ratio of n-6:n-3 PUFA between boars with normal and low motility sperm. Total lipids, cholesterol, phospholipids, PUFA, DHA and n-3 PUFA were positively correlated with sperm motility, viability, normal morphology and normal plasma membrane. In contrast, SFA and the ratio of n-6: n-3 PUFA were negatively correlated (P ≤ 0.05) with sperm motility, viability, normal morphology and normal plasma membranes. The TAS of seminal plasma from boars having normal motility sperm was higher (P ≤ 0.05) than that of boars having low motility sperm and TAS was positively correlated (P = 0.0001) with sperm motility, viability, normal morphology and normal plasma membranes. In summary, differences in sperm motility were related to n-3 PUFA content in the sperm plasma membrane and extracellular antioxidants in seminal plasma which protect sperm plasma membranes from lipid peroxidation during periods of oxidative stress. 相似文献
17.
Investigated were the changes in fatty acid composition, oxidation and enzymatic deterioration of lipids in frozen (−30°C) fish fillets from the Persian Gulf. The narrow barred Spanish mackerel ( Scomberomorus commersoni ) and white cheek shark ( Carcharhinus dussumieri ) were tested with storage times of 0, 1, 2, 3, 4, 5 and 6 months at −18°C. Statistical results showed that the major fatty acids among the saturated and monounsaturated fatty acids of each fish species were palmitic (C16:0) and oleic (C18:1n-9) acids, respectively. Both linoleic acid (C18:2n-6) and arachidonic acid (AA) (C20:4n-6) were predominant in total n-6 polyunsaturated fatty acids in both mackerel and shark. The EPA (eicosapentaenoic acid; C20:5 n-3) and DHA (docosahexaenoic acid; C22:6 n-3) acids were the major fatty acids among total n-3 acids in both fishes. During frozen storage, the PUFA (40.1 and 23.94%), n-3 (48 and 42.83%), ω 3/ ω 6 (41.36 and 50%), PUFA/SFA (56 and 42.23%) and EPA + DHA/C16 (55.55 and 46.66%) contents decreased in S. commersoni and C. dussumieri , respectively. Also peroxide, thiobarbituric acid (TBA) and free fatty acid (FFA) values significantly increased (P < 0.01) with the time of storage. 相似文献
18.
Brazão S Morais S Boaventura D Ré P Narciso L Hawkins SJ 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2003,136(3):425-441
This study evaluated the effects of season and spatial distribution on the fatty acid composition of Patella depressa gonads and Patella spp. soft body tissue. The results show that the quantitatively most important fatty acids were the saturated fatty acids (SFA) 16:0, 14:0 and 18:0; the monounsaturated fatty acids (MUFA) 18:1(n-7), 18:1(n-9), 16:1(n-7) and 20:1(n-9) and the polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (EPA 20:5(n-3)), and arachidonic acid (ARA 20:4(n-6)). P. depressa and P. ulyssiponensis soft body fatty acid profiles revealed significant differences between sexes; males showed significantly higher percentages of PUFA, highly unsaturated fatty acids (HUFA), (n-3) fatty acids and ARA, while in females significantly higher proportions of MUFA were found. Analysis of variance on the fatty acid composition of P. depressa gonads revealed significant differences between sexes, which were more marked than when the whole body was analysed. Males showed a significantly higher percentage of PUFA, HUFA, fatty acids from the (n-3) and (n-6) series, ARA and EPA, while females were seen to have higher proportions of SFA, MUFA and total fatty acid methyl esters (FAME). Some variability was seen to occur due to shore location and seasons, but these effects were not so obvious. 相似文献
19.
Fatty acid composition in photosynthetic products of natural phytoplankton population in Lake Biwa, Japan 总被引:1,自引:0,他引:1
Hama Takeo; Matsunaga Katsuji; Handa Nobuhiko; Takahashi Mikio 《Journal of plankton research》1992,14(8):1055-1065
Fatty acid composition of phytoplankton photosynthetic productswas determined by a 13C tracer and gas chromatography-mass spectrometry(13C-GC-MS) method from August 1985 to June 1986 in Lake Biwa,Japan. The total fatty acid production rate varied from 2.8to 10.9 µg C l1 day1 at the water surfaceand accounted for 9.130% of photosynthetic productionof particulate organic carbon. A high contribution of fattyacid to the particulate organic carbon production rate was noticedduring winter time, and an increase in the fatty acid contributionresulted in an increase in the C/N value in the photosyntheticproducts. The fatty acid composition varied throughout the year,mainly depending on the seasonal change in the dominant phytoplanktonspecies. The contribution of polyunsaturated fatty acids tototal fatty acids was low during the summer period, probablydue to nitrogen limitation of phytoplankton growth. 相似文献
20.
Mustonen AM Käkelä R Nieminen P 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2007,147(4):903-910
Fatty acid (FA) composition in the intraabdominal (IAB), subcutaneous (SC) and peripheral adipose tissues of the semiaquatic American mink (Mustela vison) was examined in comparison to the diet by gas-liquid chromatography. There was a clear compositional gradient from the IAB via SC to peripheral adipose tissues and the anatomically different adipose tissues accumulated or metabolized FA selectively. The total lipids of the body appendages had smaller proportions of saturated (SFA) and larger proportions of monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA) than the lipids of the trunk adipose tissues. Especially n-3 PUFA were enriched in the periphery. The appendages were also characterized with a high ratio of unsaturated FA to SFA, an increased Delta9-desaturation index and increased mean numbers of double bonds and carbon atoms in a FA molecule. The proportions of SFA and MUFA of the diet resembled the trunk adipose tissues while the dietary percentage of n-3 PUFA surpassed those of the trunk fat depots but was lower than those of the peripheral fats. These data confirm that the FA signatures of mammals reflect not only their dietary history but also metabolic modifications of ingested FA. 相似文献