赤爮亚族植物营养器官的比较解剖

对赤　亚族３属共１２种植物的营养器官进行了比较解剖学研究,其结果是：１．在卷须的解剖结构上,赤　属和白兼果属有明显的区别。前者具周维管纤维,但其维管束发育很差;后者不具周维管纤维,但其维管束发育良好。从系统演化上来说,完善的维管系统显然比周维管纤维有更强的机械功能。２．草酸钙盐的结晶体、硅质细胞和钟乳体分别存在于白兼果属、罗汉果属和苦瓜属植物中,而在赤　属植物中未发现上述结构物质。３．比较解剖学的证据部分地支持了赤属和白兼果属的近缘关系以及赤　属是赤　亚族中相对原始的分类群的观点。     

贵州悬钩子属(蔷薇科)一新种--务川悬钩子

报道了贵州省务川县发现的悬钩子属Rubus（蔷薇科Rosaceae）一新种——务川悬钩子R．wuchuanensis S．Z．He。该种在体态上与锯叶悬钩子R．wuzhianus L．T．Lu＆Boufford相近,区别在于其叶片卵状披针形,边缘疏生小锯齿;叶柄较短,长0．7-1cm;花序为顶生稀疏总状花序,具花8-10朵：花梗长3．55cm;花瓣先端具骤突尖头。     

新疆翠雀属(毛茛科)一新种——文采新翠雀花

报道了产于中国新疆天山的翠雀属(毛茛科)一新种——文采新翠雀花Delphinium neowentsaii C. Y. Yang。该种的叶为肾形,叶柄基部不扩展,花较小,组成稀疏总状花序,花梗被贴伏短柔毛,上萼片的距圆锥状钻形,长13-15 mm,基部宽约3 mm,外面疏被短柔毛,萼片蓝色,与西伯利亚的疏花翠雀花D. laxiflorum DC.很相似,但其花瓣和退化雄蕊均为淡黄色而易于区别。     

Croton dissectistipulatus, a new species of Euphorbiaceae from Amazonian Brazil

A new species ofCroton,C. dissectistipulatus, is described from Amazonian Brazil. This species is superficially similar toC. timandroides from northeastern and southeastern Brazil, but differs in having petiolate leaves with glandular margins, persistent stipules, conspicuous racemose inflorescences, staminate flowers having externally glabrous sepals and 3 stamens, and pedicellate pistillate flowers. The systematic position ofC. dissectistipulatus relative to the sections ofCroton is discussed.     

圆盔乌头, 陕西毛茛科一新种

描述了自陕西省发现的毛茛科乌头属一新种,圆盔乌头（Aconitum rotundocassideum）。由于具有相似的体态和盔形上萼片,此种与特产云南省的粗茎乌头在亲缘上很相近,与后者的区别特征见正文。     

Stirtonia, a new genus of the tribe Podalyrieae (Leguminosae) from South Africa

The genus Stirtonia is described to accommodate three anomalous species, hitherto placed in Podalyria . The new genus differs from Podalyria mainly in the decussate (geminate) inflorescence structure (racemose in Podalyria ), the yellow flower colour (purple, pink or white in Podalyria ), the non-fleshy rim-aril of the seeds (seed aril invariably fleshy and collar-like in Podalyria ) and in the diagnostically different combination of quinolizidine alkaloids. The major alkaloidal metabolites are carboxylic acid esters of lupanine and virgiline, but these compounds are totally absent in Podalyria . The morphology of inflorescences, leaves, flowers and seeds are described and illustrated. Chromosome numbers (2n = 18) are reported for the first time. A formal taxonomic treatment of the three species, S. tayloriana, S. chrysantha and S. insignis , is presented.     

Notulae De Ranunculaceis Sinensibus(ⅩⅩⅡ)

(1) In the overwhelming majority of genera of the family Ranunculaceae, includ ing its primitive genera, Caltha, Calathodes, and Trollius and the primitive genus of trib. Anemoneae, Anemone, the sepals are spreading and the stamens are glabrous. So, the as cending or upright sepals and hairy stamens of the sections Meclatis, Tubulosa, Viorna, and Atragene of the genus Clematis are secondary, and are accordingly considered as advanced characters, and those sections and the genus Archiclematis, closely related to Sect. Viorna Subsect. Connatae, more or less advanced groups. (2) In the sections Cheiropsis, Fruticella, and Viticella, which have glabrous stamens,some species have spreading sepals, and the others have ascending or upright sepals. In Sect. Clematis, all the species have spreading sepals and glabrous stamens, except for Clematis pinnata, which has ascending sepals and usually hairy stamen filaments. In Sect. Lasiantha with 2 species restricted to western U. S. A., C. lasiantha has glabrous stamens, while C. paucifiora has stamens hairy on fliaments. In Sect. Naraveliopsis with spreading sepals,the majority of species have glabrous stamens, but one species, C.liboensis, endemic to Guizhou Province, China, has hairy stamens. These facts just mentioned indicate that the evolution of sepals and stamens took place in several lineages independently in Clematis. (3) In Clematis, glabrous stamens of C.apiifolia, C.grata, and C.montana with linear filaments and oblong anthers, are similar to those of Caltha, Calathodes, Trollius, and Anemone. Thus, the linear filaments and oblong anthers are considered primitive characters in Clematis. On the other hand, lanceolatelinear filaments of C. tangutica and C. aethusifolia or oblanceolate -linear filaments of C. courtoisii and C. loureiriana and linear anthers of C. meyeniana and C. uncinata, and narrow-linear anthers of C. courtoisii and C. lanuginosa are considered advanced ones. In ease of stamens with hairs, stamens of C. henryi with densely villous filaments and those of C. kweichowensis with both filaments and anthers densely pubescent show more advanced condition than those of C. pinnata, C. heracleifolia, and C. tangutica, with sparsely puberulous filaments and glabrous antbers(Fig. 1 ). (4)The pedunculate, 2-bracteate dichasial cyme with several flowers may represent the primitive type of inflorescences in Clematis. Manyflowered panicle-like cymes as in C.gouriana and C. tsaii, or few-l-flowered cymes as in C. henryi and C. repens, and cymes lacking peduncles and bracts as in C. montana and C. pogonandra are all considered advanced. Besides, the fact that flowers arise from axillary buds of old branches shows also an advanced condition. (5)Sect. Clematis subsect. Pinnatae, with leaflets, inflorescence ramification, and stamens similar to those of C. heracleifolia, is considered intermediate between Sect. Clematis and Sect. Tubulosa. (6) Subsect. Clematis and Subsect. Rectae, and Subsect. Connatae and Subsect. Crispae are so closely related to each other respectively that it is difficult to ascertain the systematic position of some intermediate species between the two subsections of each pair in the absence of seedlings. So, in the present paper, following the classification of Clematis proposed by Tamura in 1967, I put Subsect. Clematis and Subsect. Rectae in Sect. Clematis, and Subsect. Connatae and Subsect. Crispae in Sect. Viorna. (7)According to the evolutionary tendencies mentioned above, a realignment of the sections and the infrasectional taxa of the Chinese Clematis is made. (8) Six subsections, 6 serise, 2 species, and 4 varieties are described as new, and 5 new combinations, 4 new ranks, and 2 new names are given. (9)The specific rank of C. tenuipes W.T. Wang, reduced to varietal renk in 1980, is restord. C. taiwaniana Hayata, reduced to synonomy of C. grata Wall. in 1991, is considered distinct from the latter in hairy adaxial surface of sepal and narrower achene with tapering apex. C. kerriana Drumm. & Craib and C. laxipaniculata Pei are proved to be conspecific to C. subumbellata Kurz and reduced to syn-onymy.     

圆盔乌头，陕西毛茛科一新种

描述了自陕西省发现的毛莨科乌头属一新种,圆盔乌头（Aconitum rotundocassideum）。由于具有相似的体态和盔形上萼片,此种与特产云南省的粗茎乌头在亲缘上很相近,与后者的区别特征见正文。     

Observations on the Chromosome Number of Siraitia grosvenori in China

A cytological study reveals the chromosome number of 2n=28 for Siraitia grosvenori (Swingle) C. Jeffrey ex Lu et Z. Y. Zhang from Yongfu County, Guangxi (fig. 1), which is different from the previous report of 2n=24 (Zou Qi-li et al. 1980). The voucher is deposited in the herbarium of Commission for Integrated Survey of Natural Resources,Academia Sinica.     

靖西铁线莲, 广西毛茛科一新种

该文描述了自广西发现的毛茛科铁线莲一新种,靖西铁线莲,此新种与宝岛铁线莲在亲缘关系上接近,与后者的区别在于本种的茎疏被短柔毛,小叶卵形或狭卵形,不分裂,聚伞花序有1～3花,萼片较大长1.7～2 cm,宽0.5～0.7 cm。     

Impatiens maculifera sp. nov. (Balsaminaceae) Yunnan,China

Impatiens (Balsaminaceae) is a notoriously difficult genus to study for reasons that include small, remote and endemic areas of occurrence, and difficulty in obtaining well‐dried herbarium specimens and separating and reconstructing floral parts from available dried specimens. Impatiens has also been notoriously difficult when it comes to phylogenetic resolution at the infrageneric level, but a new system with two subgenera (Impatiens and Clavicarpa) was recently proposed by Yu et al. and it has made it easier to classify newly discovered species. Impatiens maculifera, a new species of Balsaminaceae from Malipo County, Yunnan, China described here, is similar to I. parvisepala S.X. Yu & Y.T. Hou in having racemose inflorescences, four lateral sepals, clavate capsules and ellipsoid seeds, but differs in having leaves narrowly oval or with elliptic–lanceolate blades (versus obovate or obovate–lanceolate), obvious petioles (versus leaves subsessile), racemose inflorescence with 8–12 flowers (versus 6–8 flowers), flowers greenish yellow (versus yellow), lateral united petals and lower sepal with dense red spots (versus red spots absent), and dorsal petal with apparent stalk at base (versus unapparent stalk). Morphological characters and molecular phylogenetic analyses of DNA sequences from both nuclear ribosomal and plastid genes show that the new species differs distinctively from I. parvisepala. Furthermore, I. maculifera is distinguished from other Impatiens species based on morphological, micromorphological and palynological evidence, and molecular data.     

Notulae de Ranunculaceis sinensibus (ⅩⅩⅢ)

(1) In this paper, differences among the five genera constituting the tribe Cimi cifugeae of the family Ranunculaceae are discussed. Beesia, the first genus, with compound cymes and flowers bearing neither petals nor staminodes, is different from the other four genera with simple or compound racemes and flowers bearing either petals or staminodes, and may occupy a primitive position within the tribe. As to the other four genera, Souliea is characterized by the stem without basal leaf but with 2～5 sheath-like cataphylls, the sepals being deciduous but not caducous, moderate in size and petaloid, the petals being much smaller than sepals, but pink in color and more or less petaloid, the pollen grains being pan tocolpate or pantoporate, the carpels being 1～3 per flower, when mature forming dry linear follicles conspicuously reticulate on the surface and dehiscent along the ventral suture, and the seeds being reticulate-foveolate on the surface. These diagnostic characters indicate clear ly that Souliea might have deviated from the lineage formed by the next three genera, i. e. Anemopsis, Cimicifuga, and Actaea, which have their own well-recognizable diagnostic characters. Anemopsis is characterized by the normally developed basal leaf, the racemose inflorescence with sparse and few long pedicellate flowers, the sepals 7～10 in number, mod erate in size, and petaloid, the petals slightly smaller than sepals, the tricolpate pollen grains, the carpels 2～4 per flower, stalked, when mature forming dry oblong follicles with transverse veins on the surface, and the seeds with scaly membranous wings. Cimicifuga is distinguished by the normally developed basal leaf, the caducous, small, often sepaloid sepa ls, the organs of the second floral whorl sometimes with empty sterile anthers being stamin odes not petals, the tricolpate pollen grains, the carpels 1～8 per flower, when mature form ing dry oblong or ovoid follicles with transverse veins on the surface, and the seeds usually with scaly membranous wings. The last genus Actaea is different by the basal leaf trans formed into a small scale, the caducous, small, often sepaloid sepals, the organs of the sec ond floral whorl being clawed petals, the pollen grains with 3(4～6) colpi, carpel 1 per flow er, when mature forming a fleshy indehiscent berry smooth on the surface and without any veins, the seeds roughish or slightly rugose, neither foveolate nor winged on the surface, and the advanced most asymmetric karyotype. According to the diagnostic characters given above, we believe that Beesia, Souliea, Anemopsis, Cimicifuga, and Actaea do represent five independent genera, and the treatment of the tribe Cimicifugeae including these five genera in it by Hutchinson (1923), Janchen (1949) and some other authors, has precisely shown the taxonomic diversity within the tribe. We are therefore unable to accept the treatment published by Compton et al. (1998) to lump the two genera, Souliea and Cimicifuga, into the genus Actaea. (2) Compton et al. (1998, 1997) found out that the Chinese plants previously identified by various authors as Cimicifuga foetida L., in which the terminal and lateral racemes of the compound raceme flower more or less simultaneously, differ from the true C. foetida L. in northern Asia, in which the terminal raceme of the compound raceme flowers before the lateral ones, and thus restored the species name Cimicifuga mairei Lévl. , which was formerly reduced to the synonymy of C. foetida L. , for the Chinese plants. After examining the specimens collected from Siberia and from Southwest China we failed to find out any other differences in both vegetative and reproductive organs between the plants of the two regions, and we consider that it is better to treat the populations in Southwest and Central China as a geographical variety of Cimicifuga foetida L. A new combination, Cimicifuga foetida L. var. mairei (Lévl.) W. T. Wang & Zh. Wang, is thus made. (3) 3 species of Delphinium, 1 species and 1 variety of Clematis are described as new.     

AFLP用于构建罗汉果DNA指纹图谱及其幼苗雌雄鉴别

对罗汉果(Siraita grosvenorii(Swingle)C．Jeffrey ex Lu et Z．Y．Zhang)的几个品种进行AFLP标记分析,从中筛选出2个引物组合,其中一个能够用于构建不同品种的指纹图谱,另一个可以结合数量性状分析成功地鉴别罗汉果幼苗的雌雄。建立了一种可用于罗汉果幼苗雌雄鉴别的方法,并可为品种的鉴别提供依据。     

茸毛赤瓟种子不同发育时期脱水耐性和发芽力的初步研究

茸毛赤瓟种子自花后30 d发育至55 d,发芽率、发芽指数和活力指数由0升至最大;含水量逐渐下降,但下降速率不等,发育后期存在显著的成熟脱水期。花后45 d果实干重接近最大,种子干重在45 d达到最大,种子和果实的发育基本同步。自然风干1d后,花后40~50 d的种子含水量下降2%~4%。花后40 d的种子发芽力显著提高,花后45~50 d的种子无明显变化,继续干燥,发芽率、发芽指数和活力指数均有不同程度的降低,而花后50 d的种子直到含水量低至4%后才明显下降;花后35 d和55 d的种子经过不同天数干燥后,发芽力均下降。不同发育时期茸毛赤瓟种子耐脱水力有差别,由强至弱依次为花后50、45、55、40、35 d。用半致死含水量可准确地反映不同发育时期茸毛赤瓟种子的脱水敏感性的强弱。     

唐松草属六新种

描述了毛茛科唐松草属六新种:(1)大关唐松草,发现自云南东北部,与多枝唐松草甚为近缘,区别为其小叶较厚,较大,多呈卵形或宽卵形,心皮花柱较短,稍向后弯曲,瘦果呈新月形。(2)宽柱唐松草,发现自甘肃南部,其特征为心皮花柱扁平,近长圆形,腹面无柱头或柱头组织,据此特征可与此属中国其他种区别。(3)六脉萼唐松草,发现自四川中部,与白茎唐松草近缘,区别为其茎和小叶有毛,萼片具六条脉,一些雄蕊的花药败育,子房被短柔毛,柱头无翅。(4)吉隆唐松草,发现自西藏南部,与白茎唐松草甚为近缘,区别为小托叶卵形、急尖,复单歧聚伞花序一条顶生,无侧生者,雄蕊花药呈狭长圆形,顶端钝,无短尖头。(5)螺柱唐松草,发现自云南西北部,可能与白茎唐松草有亲缘关系,区别为其萼片具1条脉,花有4~5枚雄蕊,心皮花柱顶部螺旋状弯曲,柱头不明显。(6)札达唐松草,发现自西藏西南部,与多叶唐松草近缘,区别为其小叶顶端急尖,边缘具尖牙齿,聚伞圆锥花序具少数分枝和少数花,萼片狭卵形,花药狭长圆形。     

Glandular hairs in the genusDrosera (Droseraceae)

On the leaves and sepals of 52 species, representing all sections of the genusDrosera except one, 14 different types of glandular hairs were found: two-celled papillae, peltate scales, several types with unbranched, bi- or multiseriate stalk with a two- or multicellular gland, and one type with a multiseriate stalk and a two-armed gland. The combination of these hairs and the presence of non-glandular hairs confirm the actual classification of the genus. In combination with simple morphological characters (e.g., the type of insertion of the petiole) glandular hairs facilitate the identification of species even in the pharmaceutically important cut crude drug.     

毛茛科六新种

该文描述了毛茛科六新种：(1)托里乌头,与阿尔泰乌头近缘,区别为其茎和叶无毛,总状花序极密集,雄蕊花丝多有1或2小齿。(2)门源翠雀花,与大通翠雀花近缘,区别为其茎极短,花序总状,退化雄蕊瓣片不分裂。(3)云台山铁线莲,与裂叶铁线莲近缘,区别为其叶为二回羽状复叶,花较小,雄蕊花丝呈黑色,无脉。(4)黑丝铁线莲,与前种云台山铁线莲在亲缘关系上极为相近,区别为其小叶较小,多呈狭卵形或披针形,聚伞花序也较小,通常只具3花。(5)五台山毛茛,与砾地毛茛相似,区别为其基生叶被柔毛,花瓣蜜槽具鳞片,心皮具短而粗的花柱和小柱头。(6)靖西铁线莲,与宝岛铁线莲在亲缘关系上接近,区别为其茎疏被短柔毛,小叶卵形或狭卵形,不分裂,聚伞花序有1~3花,萼片较大(长1.7~2 cm,宽0.5~0.7 cm)。     

Comparative floral structure and systematics in Crossosomatales (Crossosomataceae, Stachyuraceae, Staphyleaceae, Aphloiaceae, Geissolomataceae, Ixerbaceae, Strasburgeriaceae)

Floral structure of all putative families of Crossosomatales as suggested by molecular studies was comparatively studied. The seven comprise Crossosomataceae, Stachyuraceae, Staphyleaceae, Aphloiaceae, Geissolomataceae, Ixerbaceae, and Strasburgeriaceae. The entire clade (1) is highly supported by floral structure, also the clades (in sequence of diminishing structural support): Ixerbaceae/Strasburgeriaceae (2), Geissolomataceae/Ixerbaceae/Strasburgeriaceae (3), Aphloiaceae/Geissolomataceae/Ixerbaceae/Strasburgeriaceae (4), and Crossosomataceae/Stachyuraceae/Staphyleaceae (5). Among the prominent floral features of Crossosomatales (1) are solitary flowers, presence of a floral cup, imbricate sepals with outermost smaller than inner, pollen grains with horizontally extended endoapertures, shortly stalked gynoecium, postgenitally united carpel tips forming a compitum, stigmatic papillae two‐ or more‐cellular, ovary locules tapering upwards, long integuments forming zigzag micropyles, cell clusters with bundles of long yellow crystals, mucilage cells, seeds with smooth, sclerified testa and without a differentiated tegmen. Clade (2) is characterized by large flowers, petals forming a tight, pointed cone in bud, stamens with long, stout filaments and sagittate anthers, streamlined, conical gynoecium, antitropous ovules, rudimentary aril, lignified, unicellular, T‐shaped hairs and idioblasts with striate mucilaginous cell walls. Clade (3) is characterized by alternisepalous carpels, punctiform stigma formed by postgenitally united and twisted carpel tips, synascidiate ovary, only one or two pendant ovules per carpel, nectary recesses between androecium and gynoecium. Clade (4) is characterized by pronounced ‘pollen buds’. Clade (5) is characterized by polygamous or functionally unisexual flowers, x‐shaped anthers, free and follicular carpels (not in Stachyuraceae). Crossosomataceae and Aphloiaceae, although not retrieved as a clade in molecular studies, share several special floral features: polystemonous androecium; basifixed anthers without a connective protrusion; stigma with two more or less decurrent crests; camplyotropous ovules and reniform seeds; simple, disc‐shaped nectaries and absence of hairs. © 2005 The Linnean Society of London, Botanical Journal of the Linnean Society, 2005, 147 , 1–46.     

A Study on the Genus Acidosasa of Bambusoideae

The genus Acidosasa was published by the present authors in 1979. It only had one species at that time, Acidosasa chinensis C. D. Chu et C. S. Chao. Since then species number of the genus steadily increases. The authors have rather comprehensively studied this genus and its related genera for F1. Reip. Pop. Sin. The present paper deals mainly with morphological characteristics of the genus Acidosasa and the differences from its related genera i. e. Arundinaria, Sasa and Indosasa. The genus Acidosasa is closely related to the genus Arundinaria in the type and origin of inflorescences and the vegetative appearance. But it differs from Arundinaria in the structure of florets. In Acidosasa, each floret is provided with six stamens, while in Arundinaria each floret is of only three stamens. The genus Acidosasa is similar to the genera Indosasa and Sasa in the numbers of stamens, but it is distinguished from lndosasa by its semelauctant (determinate) inflorescence, not iterautant (indeterminate) one, from Sasa by its taller stature and branch complement with three branches. We have carefully examined all the type specimens of Acidosasa and its related genera. A conclusion reached is that there are six species in the genus Acidosasa, most of which are native to S. China, with only one species in Viet Nam. Five specific binomials are reduced and one species is transferred into this genus. Two keys to species, respectively based on the flowering and vegetative characters, are given as follow: Key to species of the genus A cidosasa (1)(based on the flowering state) 1. Lemmas glabrous. 2. Spikelets stout, 3-6mm broad, pedicels 1.5-4cm long; lemmas large, 1.5-2.2cm long, with 15-19 nerves, subcoriaceous, not glaucous, shiny. 3. Lemmas up to 2.2cm long with conspicuously transverse veinlets, tessellate; palea and rhachilla entirely. glabrous, lodicules elliptic-lanceolate, glabrous ... 1. A. chinensis 3. Lemmas 1.5-1.8cm. long, slightly tessellate; palea puberulous at apex of carina, rhachilla puberuous at apex, lodicules obovate, ciliate at apex ............ 2. A. brilletii 2. Spikelets rather slende, 2-4mm broad, pedicels 0.5-1cm long; lemmas small, about 1.3 cm long, with 7-13 nerves, more or less glaucous .......... 3. A. chienouensis 1. Lemmas pubescent. 4. Glumas and lemmas densely pubescent ........................ 4. A. hirtiflora 4. Glumas subglabrous, lemmas sparsely pubescent. 5. Spikelets large, 3-7 cm long, lemmas 1.6-1.7 cm long, pedicels 2-13 mm long ................................................. 5. A. longiligula 5. Spikelets small, 2-3.7 cm long, lemmas about 1.3 cm long, pedicels 1-3 cm long ................................................... 6. A. venusta Key to species of the genus A cidosasa (2) (based on the vegetative state) 1. Ligules of leaf-sheaths strongly elevated, usually 2-8 mm long. 2. Young culms with bristly sheath scars; culm-sheaths without auricles and oral setae, not spotted, sheath-blades erect ................. 4. A. hirtiflora 2. Young culms with glabrous sheath scars; culm-sheaths with small auricles and oral setae, sparsely spotted, sheath-blades reflexed .......... 5. A. longiligula 1. Ligules of leaf-sheaths inconspicuous, less than 2 mm long. 3. Young culms more or less bristly, or sheath-scars bristly: 4. Culm-sheaths without auricles and oral setae, not farinose, without hairs at base. 5. Young culms densely bristly; culm-sheaths attenuate at apex and as wide as sheath-blades, with conspicuously transverse veinlets; leaf-blades large, usually 2.5-3.5 (-6.5) cm broad, conspicuously tessellate ..................................................... 1. A. chinensis 5. Young culms sparsely bristly; culm-sheaths truncate at apex and broader than sheath-blades, without transverse veinlets or inconspicuous; leaf-blades small, 1.5-2.5 cm broad, without visible transverse veinlets .................................................... 6. A. venusta 4. Culm-sheaths with auricles and oral setae, slightly farinose, densely bristle at base; leaf-blades rather narrow, 0.8-1.8 cm broad ............ 3. A. chienouensis 3. Young culms entirely glabrous; leaf-blades rather narrow, 1.2-1.8 cm broad ....................................................................................... 2. A. brilletill     

A comparison of in vitro flowers to in vivo flowers of haploid and diploidNicotiana tabacum L.

Thin cell layers (TCLs) were cultured from inflorescences of diploid (2n=4x=48) and haploid (2n=2x=24)Nicotiana tabacum L. "Samsun" and the subsequent flowers formed in vitro were then compared to in vivo flowers. Plants derived from TCLs possessed flowers that were typical of their seed or androgenetically-derived counterparts, whereas de novo flowers from TCLs were abnormal when compared to their counterparts. The TCLs of haploid plants produced more flower buds than diploid TCLs, and did so in a shorter period of time. In vitro flowers and anthers at both ploidy levels were considerably smaller than the in vivo flowers; in vitro flowers also had variable numbers of anthers and pistils. The embryogenic capacity of anthers taken from in vivo diploid flowers was 5 times greater than that of in vitro diploid or haploid anthers. In vivo haploid anthers produced no embryoids, whereas in vitro haploid anthers did produce embryoids. Observations of mitotic cells in root tips of plants derived from anther cultures of in vitro haploid flowers revealed a mixoploid nature. Diploid meiosis was regular and haploid meiosis was irregular regardless of the origin (in vitro or in vivo) of the flowers.Supported by state Hatch funds.