共查询到20条相似文献,搜索用时 15 毫秒
1.
It has been shown recently that prolonged blockade of neuronal firing activates several homeostatic mechanisms in neocortical networks, including alteration of glutamatergic and GABA-ergic synaptic transmission, and postsynaptic changes are involved in both cases. We studied whether such treatment also affects GABA-ergic synaptic transmission in hippocampal cell cultures. Using whole-cell voltage-clamp recording and local extracellular stimulation, we investigated evoked inhibitory postsynaptic currents (IPSC) in cultured rat hippocampal neurons grown with the sodium channel blocker tetrodotoxin (TTX) and under control conditions. We found that chronic TTX treatment significantly decreased the amplitude of evoked IPSC. This decrease was accompanied by an increase in the coefficient of variation of the above parameter, which is suggestive of a presynaptic mechanism. In contrast, no changes in the IPSC reversal potential or paired-pulse depression were observed in TTX-treated cultures. We conclude that alteration of GABA-ergic synaptic transmission contributes to the homeostatic plasticity in hippocampal neuronal networks, and this change is at least in part due to a presynaptic mechanism.Neirofiziologiya/Neurophysiology, Vol. 36, Nos. 5/6, pp. 432–437, September–December, 2004.This revised version was published online in April 2005 with a corrected cover date and copyright year. 相似文献
2.
We studied the characteristics of short-term plasticity in inhibitory synapses of cultured neurons of the rat hippocampus.
In our experiments, we used techniques of voltage clamp in the whole-cell configuration and of local electrical stimulation
(pairs of stimuli were applied to a single synaptic terminal of the GABA-ergic neuron under conditions of the blockade of
spreading excitation). We demonstrated that an increase or a decrease in the extracellular concentration of calcium ions ([Ca2+]o) results in modifications of the pattern of this plasticity. Depression of the second postsynaptic response under conditions
of normal [Ca2+]o was characterized by a paired-pulse ratio (PPR) equal, on average, to 0.78 ± 0.04 (n = 5). With a decrease in the [Ca2+]o to 0.5 mM, depression was changed to facilitation (PPR = 1.17 ± 0.08, n = 5), while with a rise in the [Ca2+]o to 5.0 mM, depression became more clearly pronounced (PPR = 0.48 ± 0.03, n = 5). Alterations of responses, which were determined by a decrease or an increase in the [Ca2+]o, differed significantly from those related to a decrease or an increase in the amplitude of presynaptic stimulation. Analysis
of the parameters of the pairs of evoked inhibitory postsynaptic currents under conditions of various [Ca2+]o and different intensities of stimulation of the presynaptic terminal allows us to conclude that in these terminals calcium-dependent
(and, probably, also voltage-dependent) mechanisms underlying control of short-term synaptic plasticity are present.
Neirofiziologiya/Neurophysiology, Vol. 38, No. 2, pp. 103–112, March–April, 2006. 相似文献
3.
Oxygen-glucose deprivation (OGD) leads to depression of evoked synaptic transmission, for which the mechanisms remain unclear. We hypothesized that increased presynaptic [Ca2+]i during transient OGD contributes to the depression of evoked field excitatory postsynaptic potentials (fEPSPs). Additionally, we hypothesized that increased buffering of intracellular calcium would shorten electrophysiological recovery after transient ischemia. Mouse hippocampal slices were exposed to 2 to 8 min of OGD. fEPSPs evoked by Schaffer collateral stimulation were recorded in the stratum radiatum, and whole cell current or voltage clamp recordings were performed in CA1 neurons. Transient ischemia led to increased presynaptic [Ca2+]i, (shown by calcium imaging), increased spontaneous miniature EPSP/Cs, and depressed evoked fEPSPs, partially mediated by adenosine. Buffering of intracellular Ca2+ during OGD by membrane-permeant chelators (BAPTA-AM or EGTA-AM) partially prevented fEPSP depression and promoted faster electrophysiological recovery when the OGD challenge was stopped. The blocker of BK channels, charybdotoxin (ChTX), also prevented fEPSP depression, but did not accelerate post-ischemic recovery. These results suggest that OGD leads to elevated presynaptic [Ca2+]i, which reduces evoked transmitter release; this effect can be reversed by increased intracellular Ca2+ buffering which also speeds recovery. 相似文献
4.
Cui LN Coderre E Renaud LP 《American journal of physiology. Regulatory, integrative and comparative physiology》2000,278(5):R1210-R1216
This study used whole cell patch clamp recordings in rat hypothalamic slice preparations to evaluate the effects of GABA(B) receptor activation on GABA(A)-mediated inhibitory postsynaptic currents (IPSCs) in paraventricular nucleus magnocellular neurons evoked by electrical stimulation in the suprachiasmatic nucleus (SCN). Baclofen induced a dose-dependent (1-10 microM) and reversible reduction in SCN-evoked IPSC amplitude (11/11 cells), blockable with 2-hydroxysaclofen (300 microM; 3/3 cells). IPSCs displayed paired-pulse depression (PPD), attenuated by both baclofen and 2-hydroxysaclofen, but neither altered resting membrane conductances or IPSC time constants of decay. Baclofen induced a significant dose-dependent (1-100 microM) reduction in frequency, but not amplitude, of spontaneous IPSCs and miniature IPSCs, reversible with 2-hydroxysaclofen pretreatment. Baclofen effects and PPD persisted in slices pretreated with pertussis toxin (PTX) and N-ethylmaleimide, implying that these GABA(B) receptors are coupled to PTX-insensitive G proteins. Responses were unaltered by barium (2 mM) or nimodipine, ruling out involvement of K(+) channels and L-type Ca(2+) channels. Thus pre- and postsynaptic GABA(B) and GABA(A) receptors participate in SCN entrainment of paraventricular neurosecretory neurons. 相似文献
5.
An in vivo Ca2+ imaging technique was applied to examine the cellular mechanisms for attenuation of wind sensitivity in the identified primary sensory interneurons in the cricket cercal system. Simultaneous measurement of the cytosolic Ca2+ concentration ([Ca2+]i) and membrane potential of a wind‐sensitive giant interneuron (GI) revealed that successive air puffs caused the Ca2+ accumulation in dendrites and diminished the wind‐evoked bursting response in the GI. After tetanic stimulation of the presynaptic cercal sensory nerves induced a larger Ca2+ accumulation in the GI, the wind‐evoked bursting response was reversibly decreased in its spike number. When hyperpolarizing current injection suppressed the [Ca2+]i elevation during tetanic stimulation, the wind‐evoked EPSPs were not changed. Moreover, after suprathreshold tetanic stimulation to one side of the cercal nerve resulted in Ca2+ accumulation in the GI's dendrites, the slope of EPSP evoked by presynaptic stimulation of the other side of the cercal nerve was also attenuated for a few minutes after the [Ca2+]i had returned to the prestimulation level. This short‐term depression at synapses between the cercal sensory neurons and the GI (cercal‐to‐giant synapses) was also induced by a depolarizing current injection, which increased the [Ca2+]i, and buffering of the Ca2+ rise with a high concentration of a Ca2+ chelator blocked the induction of short‐term depression. These results indicate that the postsynaptic Ca2+ accumulation causes short‐term synaptic depression at the cercal‐to‐giant synapses. The dendritic excitability of the GI may contribute to postsynaptic regulation of the wind‐sensitivity via Ca2+‐dependent depression. © 2001 John Wiley & Sons, Inc. J Neurobiol 46: 301–313, 2001 相似文献
6.
We recorded evoked inhibitory post-synaptic currents (eIPSC) from a post-synaptic unit in a pair of synaptically connected cultured hippocampal neurons using a voltage-clamp technique in the whole-cell configuration and extracellular electrical stimulation of the pre-synaptic axon. Thirty-six neuronal pairs were examined. Dissimilar pharmacological sensitivities of eIPSC to a number of inorganic and organic blockers made it possible to estimate the involvement of different types of Ca2+ currents in Ca2+ entry into the presynaptic terminal and initiation of neurotransmitter release. Application of specific blockers of high-threshold Ca2+ channels allowed us to demonstrate that Ca2+ entry into presynaptic terminals of cultured hippocampal neurons is provided mostly by the system of high-threshold Ca2+ channels of the N- and P/Q-subtypes. The involvement of the L-subtype Ca2+ channels in the control of inhibitory transmission under study is insignificant. 相似文献
7.
Feng Yang Hyun-Soo Je Yuanyuan Ji Guhan Nagappan Barbara Hempstead Bai Lu 《The Journal of cell biology》2009,185(4):727-741
Postsynaptic cells generate positive and negative signals that retrogradely modulate presynaptic function. At developing neuromuscular synapses, prolonged stimulation of muscle cells induces sustained synaptic depression. We provide evidence that pro–brain-derived neurotrophic factor (BDNF) is a negative retrograde signal that can be converted into a positive signal by metalloproteases at the synaptic junctions. Application of pro-BDNF induces a dramatic decrease in synaptic efficacy followed by a retraction of presynaptic terminals, and these effects are mediated by presynaptic pan-neurotrophin receptor p75 (p75NTR), the pro-BDNF receptor. A brief stimulation of myocytes expressing cleavable or uncleavable pro-BDNF elicits synaptic potentiation or depression, respectively. Extracellular application of metalloprotease inhibitors, which inhibits the cleavage of endogenous pro-BDNF, facilitates the muscle stimulation–induced synaptic depression. Inhibition of presynaptic p75NTR or postsynaptic BDNF expression also blocks the activity-dependent synaptic depression and retraction. These results support a model in which postsynaptic secretion of a single molecule, pro-BDNF, may stabilize or eliminate presynaptic terminals depending on its proteolytic conversion at the synapses. 相似文献
8.
We have isolated cell wall peptidoglycan associated proteins (CW-Pr) of Mycobacterium tuberculosis H37Ra by chemical treatment with trifluoromethanesulfonic acid:anisole (2:1), which further resolved into 71, 60 and 45 kDa proteins on SDS-PAGE. A study was carried out to investigate the immunoreactivity of these proteins with blood samples from 4 categories, including 15 tuberculous patients (TB), 5 tuberculous patients on ATT (TBT), 10 PPD non-reactive healthy controls (HPPD?) and 11 PPD reactive healthy controls (HPPD+). Comparing the proliferative responses to cell wall protein antigens, it was observed that the 71 kDa protein gave maximum stimulation with PBMCs from the TB and HPPD+ groups. The adherent PBMCs from the TB group also demonstrated enhanced phagocytosis, particularly in the presence of 71 and 45 kDa proteins, and the phagocytic index was significantly higher (P < 0.05) than the TBT group. However, PBMCs from of the groups recognized the 60 kDa cell wall antigen. Our results suggest that the 71 kDa protein from the cell wall of M. tuberculosis is highly immunogenic. 相似文献
9.
Merrill J. Birdno Wei Tang Jonathan O. Dostrovsky William D. Hutchison Warren M. Grill 《PloS one》2014,9(5)
Thalamic deep brain stimulation (DBS) is an effective treatment for tremor, but the mechanisms of action remain unclear. Previous studies of human thalamic neurons to noted transient rebound bursting activity followed by prolonged inhibition after cessation of high frequency extracellular stimulation, and the present study sought to identify the mechanisms underlying this response. Recordings from 13 thalamic neurons exhibiting low threshold spike (LTS) bursting to brief periods of extracellular stimulation were made during surgeries to implant DBS leads in 6 subjects with Parkinson''s disease. The response immediately after cessation of stimulation included a short epoch of burst activity, followed by a prolonged period of silence before a return to LTS bursting. A computational model of a population of thalamocortical relay neurons and presynaptic axons terminating on the neurons was used to identify cellular mechanisms of the observed responses. The model included the actions of neuromodulators through inhibition of a non-pertussis toxin sensitive K+ current (IKL), activation of a pertussis toxin sensitive K+ current (IKG), and a shift in the activation curve of the hyperpolarization-activated cation current (Ih). The model replicated well the measured responses, and the prolonged inhibition was associated most strongly with changes in IKG while modulation of IKL or Ih had minimal effects on post-stimulus inhibition suggesting that neuromodulators released in response to high frequency stimulation are responsible for mediating the post-stimulation bursting and subsequent long duration silence of thalamic neurons. The modeling also indicated that the axons of the model neurons responded robustly to suprathreshold stimulation despite the inhibitory effects on the soma. The findings suggest that during DBS the axons of thalamocortical neurons are activated while the cell bodies are inhibited thus blocking the transmission of pathological signals through the network and replacing them with high frequency regular firing. 相似文献
10.
Cerebellar Purkinje neurons maintain high firing rates but their synaptic terminals depress only moderately, raising the question of how vesicle depletion is minimized. To identify mechanisms that limit synaptic depression, we evoked 100 Hz trains of GABAergic inhibitory postsynaptic currents (IPSCs) in cerebellar nuclear neurons by stimulating Purkinje axons in mouse brain slices. The paired-pulse ratio (IPSC(2)/IPSC(1)) of the total IPSC was approximately 1 and the steady-state ratio (IPSC(20)/IPSC(1)) was approximately 0.5, suggesting a high response probability of postsynaptic receptors, without an unusually high release probability. Three-dimensional electron microscopic reconstructions of Purkinje boutons revealed multiple active zones without intervening transporters, suggestive of "spillover"-mediated transmission. Simulations of boutons with 10-16 release sites, in which transmitter from any site can reach all receptors opposite the bouton, replicated multiple-pulse depression during normal, high, and low presynaptic Ca influx. These results suggest that release from multiple-site boutons limits depletion-based depression, permitting prolonged, high-frequency inhibition at corticonuclear synapses. 相似文献
11.
Myongkeun Oh Shunbing Zhao Victor Matveev Farzan Nadim 《Journal of computational neuroscience》2012,33(3):573-585
Although synaptic output is known to be modulated by changes in presynaptic calcium channels, additional pathways for calcium entry into the presynaptic terminal, such as non-selective channels, could contribute to modulation of short term synaptic dynamics. We address this issue using computational modeling. The neuropeptide proctolin modulates the inhibitory synapse from the lateral pyloric (LP) to the pyloric dilator (PD) neuron, two slow-wave bursting neurons in the pyloric network of the crab Cancer borealis. Proctolin enhances the strength of this synapse and also changes its dynamics. Whereas in control saline the synapse shows depression independent of the amplitude of the presynaptic LP signal, in proctolin, with high-amplitude presynaptic LP stimulation the synapse remains depressing while low-amplitude stimulation causes facilitation. We use simple calcium-dependent release models to explore two alternative mechanisms underlying these modulatory effects. In the first model, proctolin directly targets calcium channels by changing their activation kinetics which results in gradual accumulation of calcium with low-amplitude presynaptic stimulation, leading to facilitation. The second model uses the fact that proctolin is known to activate a non-specific cation current I MI . In this model, we assume that the MI channels have some permeability to calcium, modeled to be a result of slow conformation change after binding calcium. This generates a gradual increase in calcium influx into the presynaptic terminals through the modulatory channel similar to that described in the first model. Each of these models can explain the modulation of the synapse by proctolin but with different consequences for network activity. 相似文献
12.
Abstract The mechanism of the negative control of tyrosine hydroxylase (TH) activity induced by the stimulation of presynaptic 3,4-dihydroxyphenylethylamine (dopamine, DA) autoreceptors was investigated using rat striatal slices and synaptosomes incubated under control ([K+] = 4.8 mM) or depolarizing ([K+] = 60 mM) conditions. The stimulation of DA autoreceptors by 7-hydroxy-2-(di-n-propylamino) tetralin (1 μM 7-OH-DPAT) produced a significant decrease in TH activity extracted from striatal slices maintained under control conditions. This effect was associated with the complete conversion of TH into an enzyme form with a low affinity for its pterin cofactor (Km~0.80 mM). Furthermore, compared to TH extracted from control tissues, that from 7-OH-DPAT-exposed striatal slices was more sensitive to the stimulatdry effects of exogenous heparin and cyclic AMP-dependent phosphorylation. Such changes were opposite to those induced by incubating striatal slices with the adenylate cyclase activator forskolin. Indeed, forskolin treatment completely converted TH into an enzyme form with a high affinity for its pterin cofactor (Km~0.16 mM). Such conversion was associated with a shift in the optimal pH for TH activity from 5.8 (control) to 7.2 (forskolin). Under depolarizing conditions, the blockade by (—)-sulpiride of the stimulation of DA autoreceptors by endogenous DA was associated with a marked activation of TH. Modifications of enzymatic characteristics triggered by (—)-sulpiride were then similar to those induced by forskolin treatment. These data suggest that presynaptic DA autoreceptors modulate the activity of TH by controlling the degree of cyclic AMP-dependent phosphorylation of the enzyme. The blockade by Pertussis toxin of the 7-OH-DPAT-induced inhibition of TH activity is coherent with a possible negative coupling of presynaptic DA autoreceptors (closely related to the D2 type) with adenylate cyclase. Such negative coupling would account for the reduction of TH activity when presynaptic DA autoreceptors are stimulated. 相似文献
13.
Phosphoinositide (PI) and calcium metabolism were studied in guinea pig cerebral cortex synaptosomes. Mass amounts of inositol and inositol monophosphates, and the levels of free intrasynaptosomal calcium ([Ca2+]i) were measured after KCl (60 mM), after a direct cholinergic agonist carbachol (CA, 1mM), and after their combination. Inositol, inositol-1-phosphate (Ins1P), inositol-4-phosphate (Ins4P) and [Ca2+]i were measured with and without 10 mM LiCl in the incubation medium. CA-induced cholinergic stimulation elevated synaptosomal Ins4P levels by 40% but did not affect Ins1P or [Ca2+]i. On the contrary, KCl elevated Ins1P by 50% and [Ca2+]i by 40% above the resting level, and decreased inositol by 20%, whereas no alterations in Ins4P occurred. CA did not modify the response of KCl, but KCl abolished the elevation of Ins4P by CA. LiCl attenuated KCl-induced elevation of Ins1P but amplified the CA-induced elevation of Ins4P. The elevation of presynaptic [Ca2+]i was accompanied by accumulation of Ins1P but not that of Ins4P. Hence, the present results suggest that presynaptic cholinergic stimulation and KCl-induced depolarization may activate different degradation pathways of inositolphosphate metabolism. 相似文献
14.
Moskalyuk A. A. Paligin O. A. Fedulova S. A. Medvedeva Yu. V. Veselovsky N. S. 《Neurophysiology》2002,34(2-3):185-187
We analyzed in detail the quantum parameters of evoked inhibitory postsynaptic currents (eIPSC) recorded from synaptically connected cultured cortex neurons using a whole-cell patch-clamp technique. The IPSC were evoked using minimum extracellular stimulation of a presynaptic unit with a frequency of 0.2 sec-1 at the holding potential of -80 mV. Amplitude histograms for eIPSC demonstrated clearly detectable equally spaced peaks. For each histogram, we used a method based on autocorrelation analysis and Monte Carlo simulation to determine whether peaks in the amplitude histograms can result due to finite sampling from the sum of the Gaussian distributions. The autocorrelation function allowed us to measure the peak spacing (and, hence, the mean quantum size) for each histogram; this parameter was found to be 10 pA. 相似文献
15.
In co-culture of spinal cord and dorsal root ganglion (DRG) neurons, we studied at different terms of culturing postsynaptic currents in DRG neurons evoked by direct electrical stimulation of single spinal neurons using a voltage-clamp technique in the whole-cell configuration. According to the reversal potential and sensitivity to bicuculline, these currents were classified as inhibitory postsynaptic currents (IPSC) carried by Cl- ions through GABAA receptors. During neuronal development in dissociated co-culture, the amplitude of evoked IPSC and their time to peak significantly increased. The time to peak of spontaneous IPSC (sIPSC) in DRG neurons remained unchanged, while the frequency of these currents increased with increasing culturing time. It is concluded that under culturing conditions spinal neurons establish inhibitory synaptic contacts with the somata of DRG neurons, and the number of such functional contacts increases in the course of culturing. Our findings show that in dissociated co-culture the process of formation of inhibitory synapses on the axon terminals of primary afferent neurons is akin to that realized in vivo, but with dissimilar topography of distribution of such synapses. 相似文献
16.
We studied evoked inhibitory postsynaptic currents (eIPSC) using local electrical stimulation of single presynaptic terminals of cultured rat neocortical neurons. According to pharmacological and kinetic properties, these currents were qualified as GABAA-activated. Using autocorrelation analysis of distributions of the eIPSC amplitudes, which were in all cases polymodal, we examined quantal characteristics of the above eIPSC. These results were compared with the values of quantal parameters (N, p, Q, and m) of the current families obtained using approximation by binomial distribution. Amplitude histograms of spontaneous miniature IPSC recorded under conditions of the minimum quantal release of the neurotransmitter were normal (close to Gaussian) with the mode within a 10 pA range, which is very close to analogous parameters calculated using autocorrelation and binomial techniques. 相似文献
17.
Different patterns of Ca2+ signals are induced by low compared to high concentrations of P2Y agonists in microglia 总被引:1,自引:1,他引:0
Brain-resident macrophages (microglia) are key cellular elements in the preservation of tissue integrity. On the other hand, they can also contribute to the development of pathological events by causing an extensive and inappropriate inflammatory response. A growing number of reports indicate the involvement of nucleotides in the control of microglial functions. With this study on P2Y receptors in rat microglia, we want to contribute to the definition of their expression profile and to the characterisation of their signalling mechanisms leading to Ca2+ movements. Endogenous nucleotides, when applied at a concentration of 100 μM, elicited robust Ca2+ transients, thanks to a panel of metabotropic receptors comprising mainly P2Y2, P2Y6 and P2Y12 subtypes. The involvement of P2Y12 receptors in Ca2+ responses induced by adenine nucleotides was confirmed by the pharmacological and pertussis toxin sensitivity of the response induced by adenosine diphosphate (ADP). Beside the G protein involved, Gi and Gq respectively, adenine and uracil nucleotides differed also for induction by the latter of a capacitative Ca2+ plateau. Moreover, when applied at low (sub-micromolar) concentrations with a long-lasting challenge, uracil nucleotides elicited oscillatory Ca2+ changes with low frequency of occurrence (≤1 min−1), sometimes superimposed to an extracellular Ca2+-dependent sustained Ca2+ rise. We conclude that different patterns of Ca2+ transients are induced by low (i.e., oscillatory Ca2+ activity) compared to high (i.e., fast release followed by sustained raise) concentrations of nucleotides, which can suggest different roles played by receptor stimulation depending not only on the type but also on the concentration of nucleotides. 相似文献
18.
Effects of adenosine on Ca2+ entry in the nerve terminal of the frog neuromuscular junction 总被引:1,自引:0,他引:1
Robitaille R Thomas S Charlton MP 《Canadian journal of physiology and pharmacology》1999,77(9):707-714
This study aimed to test whether nerve-evoked and adenosine-induced synaptic depression are due to reduction in Ca2+ entry in nerve terminals of the frog neuromuscular junction. Nerve terminals were loaded with the fluorescent Ca2+ indicator fluo 3 (fluo 3-AM) or loaded with dextran-coupled Ca2+ green-1 transported from the cut end of the nerve. Adenosine (10-50 microM) did not change the resting level of Ca2+ in the presynaptic terminal, whereas it induced large Ca2+ responses in perisynaptic Schwann cells, indicating that adenosine was active and might have induced changes in the level of Ca2+ in the nerve terminal. Ca2+ responses in nerve terminals could be induced by nerve stimulation (0.5 or 100 Hz for 100 ms) over several hours. In the presence of adenosine (10 microM), the size and duration of the nerve-evoked Ca2+ responses were unchanged. When extracellular Ca2+ concentration was lowered to produce the same reduction in transmitter release as the application of adenosine, Ca2+ responses induced by nerve stimulations were reduced by 40%. This indicates that changes in Ca2+ responsible for the decrease in release should have been detected if the mechanism of adenosine depression involved partial block of Ca2+ influx. Ca2+ responses evoked by prolonged high frequency trains of stimuli (50 Hz for 10 or 30 s), which caused profound depression of transmitter release, were sustained during the whole duration of the stimulation, and adenosine had no effect on these responses. These data indicate that neither adenosine induced synaptic depression nor stimulation-induced synaptic depression are caused by reductions in Ca2+ entry into the presynaptic terminal in the frog neuromuscular junction. 相似文献
19.
E. A. Turovsky N. P. Kaimachnikov M. V. Turovskaya A. V. Berezhnov V. V. Dynnik V. P. Zinchenko 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2012,6(1):26-34
In non-excitable cells, several kinds of agonist-induced oscillations of cytosolic Ca2+ concentration ([Ca2+]i) are known which differ in their form and generation mechanism. The oscillation source is, as a rule, the regulation of Ca2+ mobilization from intracellular stores through inositol 1,4,5-trisphosphate (IP3) receptors (IP3R) and in some cases through ryanodine receptors (RyR). In the present work, oscillations in single mature adipocytes of mice
epididymal fat on the ninth day of cultivation are studied. Cells were stimulated by acetylcholine (ACh) or by fetal bovine
serum (FBS). ACh at a concentration of 0.1–5 μM evoked a rise in [Ca2+]i to a peak and subsequent oscillations whose peaks and troughs declined along with increasing amplitude while frequency decreased.
In most cells oscillations lasted less than 5 min. The new constant or interspike level exceeded the initial one or was equal
to it (at 1 μM ACh). The removal of ACh stopped oscillations immediately. An inhibitor of phospholipase C (U73122) or of IP3R (Xestospongin C) did not affect the pattern of responses, which means that the generation of oscillations does not depend
on IP3. At the same time, suppression of responses by ryanodine, which blocks RyR, was observed. Besides, oscillatory responses
were abolished by inhibitors of phosphatidylinositol 3-kinase, NO synthase, and cGMP-dependent protein kinase. FBS (1%) initiated
oscillations characterized by return of [Ca2+]i after each peak to the baseline level, occurring prior to stimulation, and by maintenance of roughly constant amplitude and
frequency (of the order of 1 min−1). Oscillations persisted longer (more than 15 min in 87% of cells) than with ACh. Repeated stimulation of cells by FBS revealed
a strongly reduced sensitivity after 1 h of rest, whereas responses to ACh partially restored within 3 min. Investigation
of the involvement of IP3R and RyR in FBS-induced oscillations gave completely inverse results relative to ACh and demonstrated a leading role of IP3R without a considerable contribution of RyR and of its activation pathways. With both stimuli, Ca2+ entry through the plasma membrane was necessary only as a support of oscillations. The results show that in adipocytes different
agonists can engage distinct subsystems of Ca2+ signaling, each of them generating oscillations with a specific temporal pattern. 相似文献
20.
Polyclonal responses to LPS, PPD, and DxS of spleen cells from mice expressing a X-linked B-cell defect were examined. Spleen cells from young (CBA/N × BALB/c)F1 male mice responded slightly lower to LPS, significantly lower to PPD than the cells from age-matched F1 female mice, and showed no response to DxS stimulation. This hypo- or unresponsiveness of F1 male cells to PPD or DxS could not be explained by a shift in the dose-response or time kinetics of the responding cells, and also could not be due to the defect in the function of T cells or macrophages. Suppressor T cells to polyclonal response to PPD or DxS could not be shown in F1 male spleen cells. The response of F1 male cells to PPD was dramatically improved with age but not to DxS. These results suggest that B cells responsive to DxS may belong to a distinct subpopulation from the cells responsive to LPS or PPD. 相似文献