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1.
cleavage of the murine leukemia virus protein P65–70 (MW app. = 65–70,000; the specific or precursor polypeptide) occurs after exposure of virus to 2% nonidet-P40 (NP-40; v/v) at 22°C in 10mM dithiothreitol. The cleavage occurs through an intermediate stage, where a protein P40–42, of MW app. = 40–42,000 is initially formed and then declines. Viral P65–70 contains all four antigenic determinants, while P40–42 contains the determinants for p30 and p10. The results indicate that p30 and p10 are adjacent polypeptides on P65–70 and further suggest that the proteolytic cleavage of P65–70 is specific. 相似文献
2.
C D Fitch 《Life sciences》1977,21(10):1511-1514
Chloroquine-resistant is as susceptible to chloroquine as chloroquine-susceptible when adequately exposed for short periods of time (1 hour) . In both cases 3.1 mM chloroquine causes a significant decrease in infectivity of the parasites whereas 0.31 mM chloroquine is without effect. Since there is no evidence that chloroquine has a peculiar mechanism of action , these results support the hypothesis of inadequate exposure of intracellular parasites as the cause of chloroquine resistance. 相似文献
3.
The purpose of this work was to facilitate the on-farm use of frozen semen by initially thawing the straws in laboratory treated sperm (TS) rather than on-farm control sperm (CS), as is usually done. After thawing, TS was diluted, centrifuged, and extended in skim milk for storage at +15° C until utilized 3 to 6 hours later. : immediately after preparation and addition of skim milk for TS and thawing for CS, the percentage of stained cells and abnormal cells was higher (P < 0.01) in TS than in CS. In contrast, following a 3 hour incubation, TS and CS had the same proportion of motile cells. : fertility and prolificacy of FGA + PMSG-treated ewes were slightly higher following AI (1 AI/female) with TS than with CS: 52.4% 44.2% and 155.0% 148.0%, respectively. Fertility was also higher (P < 0.01) with fresh semen than with TS, but the difference was only 9.2 points (70.3% 61.1% for the respective 798 and 242 ewes inseminated once). Prolificacy rates were similar (164.3% 167.6%). 相似文献
4.
Cyclic AMP-dependent phosphorylation of rat liver 6-phosphofructo 2-kinase, fructose 2,6-bisphosphatase 总被引:8,自引:0,他引:8
M R El-Maghrabi E Fox J Pilkis S J Pilkis 《Biochemical and biophysical research communications》1982,106(3):794-802
Incorporation of 32P from [γ-32P]ATP into a homogeneous preparation of rat hepatic 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase was catalyzed by a homogeneous preparation of the catalytic subunit of the cyclic AMP dependent protein kinase from rat liver. Approximately 2 mol of phosphate were incorporated per mol of the dimeric enzyme and this was associated with inhibition of the phosphotransferase activity and activation of the phosphohydrolase activity. Acid hydrolysis of the enzyme that was phosphorylated , revealed that only seryl residues were labeled. Fructose 2,6-bisphosphate inhibited the initial rate of phosphorylation of the enzyme. It is concluded that both activities of this bifunctional enzyme are regulated in a reciprocal manner by cyclic AMP-dependent phosphorylation and that this phosphorylation can be modulated by fructose 2,6-bisphosphate. 相似文献
5.
The zymosan particles induced a time-dependent release of the chloride-dependent arginine aminopeptidase from rat peritoneal macrophages during incubations. Intraperitoneal injections of zymosan, a streptococcal cell preparation and a -suspension caused the release of the chloride-activated arginine aminopeptidase into the peritoneal fluid. The arginine aminopeptidases obtained both from the cell cultivation media and the peritoneal washes were partly purified. The enzymes were similar with regard to the following properties: chloride activation with an optimum at physiological concentrations; strong inhibition by 10?6M -chloromercuribenzoate; similar elution properties and preferential hydrolysis of mainly the -L-aminoacyl-2-naphthylamines of arginine and lysine. The chloride-activated arginine aminopeptidase released into the media in conditions was inactivated in contrast to the enzyme released into the peritoneal fluid as a result of the intraperitoneal injections. The timing of the release of the chloride-activated arginine aminopeptidase both and suggests that the enzyme plays a role in the initial phases of inflammation. 相似文献
6.
John T. Dombrosky John A. Thomas Michael G. Mawhinney John W. Lloyd 《Life sciences》1973,13(7):795-804
Varying doses of morphine sulfate (10, 20 or 40 mg/kg daily × 10) were observed to suppress metabolic activities in the mouse prostate gland. Prostate gland fructose, an index of androgenic activity, was significantly reduced by these dose regimes of morphine (P < 0.01). Injections of morphine sulfate (20 mg/kg daily × 10) led to an inhibitition in the synthesis of both fructose?14C and sorbitol?14C from glucose?14C by the prostate gland, part of which may have been due to decreased uptake of glucose by the gland. The assimilation of 2-deoxyglucose?14C by the prostate was also reduced by morphine treatment. The actions of morphine (2 × 10?3M) on the metabolism of radioactive glucose by the mouse prostate gland likewise revealed a significant reduction in the formation of sorbitol?14C, but no decrease in fructose?14C formation. These results indicate that both the and actions of morphine can inhibit fructose metabolism in the prostate gland. 相似文献
7.
Steven J. Scheinman Gerard N. Burrow Theoharis C. Theoharides Zoe N. Canellakis 《Life sciences》1977,21(8):1143-1147
High titer antiserum to hepatic ornithine decarboxylase was prepared by employing enzyme·monospecific antibody complex as the immunizing antigen. This new antiserum preparation was successfully labeled with 125I and was found to retain its specific immune properties. Iodinated antiserum was used to precipitate thyroid ornithine decarboxylase induced by a mixture of thyroid stimulating hormone and methyl xanthine in rat thyroids . 125I-labeled antibody incorporation into the enzyme antibody complex after induction showed an increase which paralleled the increase in enzymatic activity and thus suggested synthesis of thyroid enzyme protein. 相似文献
8.
J Germershausen D Goodman E W Somberg 《Biochemical and biophysical research communications》1978,82(3):871-878
RNA (guanine-7) methyltransferase, partially purified from mycelia, catalyzed the transfer of the methyl group from S-adenosylmethionine to the 5′ terminus of both poly A(+) RNA and reovirus unmethylated mRNA. RNase T2 digestion of the methylated poly A(+) RNA from yielded the “cap” structures m 7G(5′)pppAp and m 7G(5′)pppGp in a ratio of 2:1 respectively. RNase T2 digestion of the methylated reovirus mRNA yielded m 7G(5′)pppGp exclusively. The absence of mRNA 2′-0-methyltransferase activity in the enzyme preparation is consistent with the absence of 2′-0-methylation in mRNA [Seidel, B. L. and Somberg, E. W. (1978) Arch. Biochem. Biophys. , 108–112]. This is the first isolation of an eucaryotic, cellular RNA (guanine-7) methyltransferase that has been shown to methylate homologous substrate. 相似文献
9.
Microbial transformation experiments were conducted with the antitumor lactone withaferin-A. NRRL 1393 transformed withaferin-A () to 15β-hydroxywithaferin-A () and 12β-hydroxywithaferin-A (). The hydroxylated metabolites were isolated by solvent extraction and were purified by column and thin-layer chromatography. Structures of the hydroxylated metabolites were determined by protonand carbon-13 NMR, IR and mass spectral analyses, and by the preparation of acylated derivatives. Compounds and inhibited the growth and biochemical functions of grown P-388 lymphocytic leukemic cells. 相似文献
10.
Incorporation of C14 Leucine was determined or in isolated mitochondria and microsomes of rat brain and liver after acute or chronic ethanol administration .The protein synthesis in mitochondrial and microsomal preparation was inhibited respectively by chloramphenicol and cycloeximide, specific inhibitors for the two systems tested. The experimental data demonstrate that the protein synthesis in both systems, mitochondrial and microsomal, is strongly affected only after chronic treatment which produces significant activation at the mitochondrial and microsomal level in the liver and an inhibition on the same systems of the brain.The data for protein synthesis instead show strong inhibition after acute administration, except for brain mitochondria, which are practically unaffected, while after chronic treatment no significant alterations are observed. 相似文献
11.
The objective of this study was to compare the ability of porcine blastocysts to attach to various cellular and non-cellular substrates . One hundred twenty-two hatched blastocysts were collected from 17 handmated gilts and sows at slaughter. Blastocysts were randomly assigned to one of four treatments: Minimal Essential Medium (MEM) supplemented with 10% (v/v) heat treated fetal calf serum (HTFCS), monolayers of bovine uterine fibroblasts in MEM + 10% HTFCS (Buf), monolayers of bovine testicular fibroblasts in MEM + 10% HTFCS (Btes), and MEM + 10% HTFCS exposed to uterine fibroblasts for 24 hr to condition the medium (cMEM). Embryos were cultured individually in 24 well Linbro culture plates at 37 C in a humidified gas atmosphere of 5% CO2 in air. Embryos were observed at 24 hr intervals by phase contrast microscopy (100X) and measured with an ocular micrometer. Blastocyst attachment was greater (P < .01) in Buf () compared to MEM (), cMEM (), and Btes (). Embryo diameter was greater (P < .05) 24 hr prior to attachment in Buf compared to the other treatments. In addition, trophoblast monolayers continued to proliferate for 20 days when cocultured with uterine fibroblasts. These observations suggest that uterine fibroblasts provide a superior substratum for blastocyst attachment and the maintenance of swine trophoblast cells . 相似文献
12.
N H Hunt S MacNeil T J Martin 《Biochemical and biophysical research communications》1978,81(2):581-587
The 34-amino acid NH2-terminal fragment of human parathyroid hormone synthesized according to the sequence described by Niall (1) is approximately 140 times more potent than the fragment synthesized according to Brewer (2) in activating human renal cortex adenylate cyclase. The potencies of the two peptides, relative to the effect of MRC standard bovine parathyroid hormone preparation in this system, were 5600 ± 600 (S.E.M.) units/mg and 40 ± 5 units/mg respectively. The potencies of the more active peptide and the corresponding bovine parathyroid hormone sequence were similar in this system and also in assays based upon the production of cyclic AMP by chick kidney both and . 相似文献
13.
Susan Peacock Nathan Brot Herbert Weissbach 《Biochemical and biophysical research communications》1983,113(3):1018-1025
Using the plasmid pNF1337 as template, a mRNA preparation has been obtained that directs the synthesis of fMet-Val, the N-terminal dipeptide of the β subunit of RNA polymerase. RNA polymerase holoenzyme specifically inhibits the mRNA-directed synthesis of fMet-Val showing that the autoregulation by RNA polymerase of β,β′ synthesis is at the level of translation. L factor ( gene product) stimulates the synthesis of fMet-Val from a DNA template but not from mRNA. Rifampicin has no effect on the mRNA-directed synthesis of fMet-Val or the ability of RNA polymerase to inhibit fMet-Val synthesis. 相似文献
14.
Effect of prostaglandin E2 on vascular responses of the rabbit kidney to nerve stimulation and noradrenaline, in vitro and in situ 总被引:2,自引:0,他引:2
The effect of prostaglandin E2 on vascular responses of the rabbit kidney to renal nerve stimulation and noradrenaline was examined and as a test of the hypthesis that prostaglandins of the E series may be involved in the regulation of adrenergic neuroeffector transmission. Intraarterial administration of prostaglandin E2 to the kidney caused marked inhibition of vascular responses to nerve stimulation whereas the responses to noradrenaline were not significantly altered. In the preparation, vascular responses to both nerve stimulation and noradrenaline were inhibited by prostaglandin E2 infusion, although its effect on responses to nerve stimulation was approximately twice that observed on responses to noradrenaline.It is concluded that prostaglandin E2 acts primarily at a prejunctional level of adrenergic neuroeffector transmission in the kidney, although a postjunctional effect has also been observed. 相似文献
15.
A sub-nuclear preparation capable of substantial levels of DNA synthesis has been obtained from isolated S-phase nuclei of . Nuclei were disrupted by gentle resuspension in a dextran-free medium followed by immediate addition of dextran to stabilize the liberated replication complex. Synthesis continues for at least 120 min, and appears to occur by a semi-discontinuous mechanism. Little DNA synthesis occurs in preparations obtained from G2-phase nuclei. 相似文献
16.
Uterine stage embryos collected from the hamster (8-cell) and cow (morula, early blastocyst) were monitored for development (embryo culture) and (embryo transfer) following premature removal of the zona pellucida.Removal of the zona pellucida did not significantly affect development to the blastocyst stage of (1) 8-cell hamster embryos (zonae removed by a combined enzymic-mechanical procedure), (2) bovine morulae (zonae removed by mechanical means only) (3) early bovine blastocysts (zonae removed by the enzymic-mechanical technique).Zona-free hamster embryos formed significantly fewer viable fetuses than did zona-intact embryos. The lower incidence of fetal development observed following transfer of zona-free 8-cell hamster embryos may have resulted in part from the formation of chimeras by fusion of these embryos . Such fusion was observed to occur between zona-free embryos placed in close proximity. The proportion of pregnancies resulting from transfer of bovine blastocysts cultured from zona-free morulae was similar to that of zona-intact embryos.In this study we have demonstrated that (1) enzymic and mechanical procedures used to remove zonae pellucidae from uterine-stage hamster and bovine embryos do not adversely affect subsequent development of these embryos and and (2) zonae pellucidae are not required for normal development of these embryos. These findings have implications for microsurgery of mammalian embryos and for embryo transfer. 相似文献
17.
Tyge Greibrokk Jan Hansen Ronald Knudsen Yiu-Kuen Lam Karl Folkers Cyril Y. Bowers 《Biochemical and biophysical research communications》1975,67(1):338-344
A preparation of < 100 ng of a prolactin inhibiting factor was isolated which could be essentially pure, because of symmetrical single peaks by high pressure liquid chromatography. The activity was at < 5 ng which is the highest potency reported by anyone. The paucity of < 100 ng/80,000 hypothalami necessitates patience for definitive data on more product from 240,000 to 450,000 hypothalami. Weight was estimated by comparing UV absorption at 220 nm with that of synthetic peptides. This preparation is not a catecholamine by chromatography, and gives new and timely credence to the concept that prolactin secretion is mediated by complex mechanisms including a peptide inhibiting factor and a catecholamine. 相似文献
18.
We have investigated the effect of extracellular magnesium ions on the function of beta adrenergic receptors in human lymphocytes. We examined adenylate cyclase stimulation by isoproterenol displacement of (-) (3H) dihydroalprenolol from beta receptor sites, and down regulation (desensitization) of beta receptors by prolonged exposure of the cells to isoproterenol. Contrary to results obtained using broken cell preparation, in none of these situations did the presence or absence of extracellular magnesium ions make any difference. The importance of selecting the most nearly physiological preparations for conducting studies that may be extrapolated to the whole organism is stressed. 相似文献
19.
Malcolm MacCoss Eung K. Ryu Tatsuo Matsushita 《Biochemical and biophysical research communications》1978,85(2):714-723
Recently, 1-β--arabinofuranosylcytosine-5′-diphosphate--1,2-dipalmitin (VIa) was reported to inhibit the growth of L51784 cells in mice and of human colon carcinoma HCT-15 cells, also in mice. This paper describes the synthesis of a single diastereomer by conversion of 1-β--arabinofuranosylcytosine 5′-monophosphate (II) to the nucleoside 5′-phosphomorpholidate (III), followed by reaction with -α-dipalmitoylphosphatidic acid (IV) to give 1-β--arabinofuranosylcytosine-5′-diphosphate--1,2-dipalmitin (V) in good yield. The separation of the product is described and its characterization by chromatography, elemental analysis, and spectroscopic methods. The lipophilic nature of V renders it insoluble in aqueous media and a method of sample preparation utilizing sonication techniques is described which provides a clear solution suitable for biological evaluation. In addition, the ability of V to inhibit the growth of L1210 cells and of mouse myeloma MPC 11 cells is desscribed and compared with 1-β--arabinofuranosylcytosine (I) and other lipophilic prodrugs of I. 相似文献
20.
The effect of morphine sulfate (MS) on adenylate cyclase (AC) and phosphodiesterase (PDE) activities in the rat striatum was investigated. MS produced a dose-dependent increase in basal AC activity and did not alter sodium fluoride-induced stimulation both (7.5–30 mg/kg, 1 hr pretreatment, i.p.) and (1–100μM). , when submaximal effective concentrations of dopamine and MS were combined, there was an additive effect. However, administration of MS did not alter dopamine-induced stimulation of AC activity. MS, and inhibited PDE activity in a dose-dependent manner only with the high substrate concentration (3.3 × 10−3M cyclic AMP). Preliminary results from this study indicate that morphine affects the cyclic AMP system. 相似文献