Enhanced evasion of neutralizing antibody response by Omicron XBB.1.5, CH.1.1, and CA.3.1 variants

1. Download : Download high-res image (288KB)
2. Download : Download full-size image

     

Alarming antibody evasion properties of rising SARS-CoV-2 BQ and XBB subvariants

1. Download : Download high-res image (235KB)
2. Download : Download full-size image

     

Neutralization of SARS-CoV-2 Omicron sub-lineages BA.1, BA.1.1, and BA.2

1. Download : Download high-res image (281KB)
2. Download : Download full-size image

     

Rapid escape of new SARS-CoV-2 Omicron variants from BA.2-directed antibody responses

1. Download : Download high-res image (213KB)
2. Download : Download full-size image

     

Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variants

1. Download : Download high-res image (177KB)
2. Download : Download full-size image

     

Potent Omicron-neutralizing antibodies isolated from a patient vaccinated 6 months before Omicron emergence

1. Download : Download high-res image (239KB)
2. Download : Download full-size image

     

Durability of neutralization against Omicron subvariants after vaccination and breakthrough infection

1. Download : Download high-res image (196KB)
2. Download : Download full-size image

     

Progressive loss of conserved spike protein neutralizing antibody sites in Omicron sublineages is balanced by preserved T cell immunity

1. Download : Download high-res image (153KB)
2. Download : Download full-size image

     

Antibody evasion of SARS-CoV-2 Omicron BA.1, BA.1.1, BA.2, and BA.3 sub-lineages

1. Download : Download high-res image (184KB)
2. Download : Download full-size image

     

Improved HIV-1 neutralization breadth and potency of V2-apex antibodies by in silico design

1. Download : Download high-res image (200KB)
2. Download : Download full-size image

     

SARS-CoV-2 variant B.1.1.7 is susceptible to neutralizing antibodies elicited by ancestral spike vaccines

1. Download : Download high-res image (123KB)
2. Download : Download full-size image

     

Antibody escape of SARS-CoV-2 Omicron BA.4 and BA.5 from vaccine and BA.1 serum

1. Download : Download high-res image (174KB)
2. Download : Download full-size image

     

Spike recognition and neutralization of SARS-CoV-2 Omicron subvariants elicited after the third dose of mRNA vaccine

1. Download : Download high-res image (155KB)
2. Download : Download full-size image

     

Characterization of the enhanced infectivity and antibody evasion of Omicron BA.2.75

1. Download : Download high-res image (265KB)
2. Download : Download full-size image

     

SARS-CoV-2 variants B.1.351 and P.1 escape from neutralizing antibodies

1. Download : Download high-res image (306KB)
2. Download : Download full-size image

     

HIV-1 neutralizing antibodies elicited in humans by a prefusion-stabilized envelope trimer form a reproducible class targeting fusion peptide

1. Download : Download high-res image (422KB)
2. Download : Download full-size image

     

Determinants of Spike infectivity,processing, and neutralization in SARS-CoV-2 Omicron subvariants BA.1 and BA.2

1. Download : Download high-res image (239KB)
2. Download : Download full-size image

     

Structural basis for accommodation of emerging B.1.351 and B.1.1.7 variants by two potent SARS-CoV-2 neutralizing antibodies

1. Download : Download high-res image (234KB)
2. Download : Download full-size image

     

High-resolution melting analysis to discriminate between the SARS-CoV-2 Omicron variants BA.1 and BA.2

High-resolution melting (HRM) analysis was conducted to discriminate between SARS-CoV-2 Omicron variant BA.1 (B.1.1.529.1) and subvariant BA.2 (B.1.1.529.2). We performed two-step PCR consisting of the first PCR and the second nested PCR to prepare the amplicon for HRM analysis, which detected G339D, N440K, G446S and D796Y variations in the SARS-CoV-2 spike protein. The melting temperatures (Tms) of the amplicons from the cDNA of the Omicron variant BA.1 and subvariant BA.2 receptor binding domain (RBD) in spike protein were the same: 75.2 °C (G339D variation) and 73.4 °C (D796Y variation). These Tms were distinct from those of SARS-CoV-2 isolate Wuhan-Hu-1, and were specific to the Omicron variant. In HRM analyses that detected the N440K and G446S variations, the Tms of amplicons from the cDNA of the Omicron variant BA.1 and subvariant BA.2 RBDs were 73.0 °C (N440K and G446S variations) and 73.5 °C (G446S variation). This difference indicates that the SARS-CoV-2 Omicron variants BA.1 and BA.2 can be clearly discriminated. Our study demonstrates the usefulness of HRM analysis after two-step PCR for the discrimination of SARS-CoV-2 variants.