Hippodamia variegata (Goeze) (Coleoptera: Coccinellidae), a predacious ladybird new in Australia

Abstract The originally Palaearctic but now widespread coccinellid Hippodamia variegata was recorded for the first time in Australia at Gatton, Queensland, in November 2000 . Since then, it has been recorded preying on 12 different aphid species and a psyllid, on a variety of crops, weeds and ornamental plants. Within a year of its discovery, it had been recorded within a distance of 800 km north and south and 500 km west of the original collection point. Collection and observation records are presented.     

The ladybird beetles (Coleoptera,Coccinellidae) of Moscow Province

Data on the fauna and ecology of the ladybird beetles (Coleoptera, Coccinellidae) of Moscow Province are summarized. In total, 60 species of 29 genera are recorded for this region. The paper is provided with 30 original color photographs of 29 species representing all the genera known from Moscow Province.     

C-value estimates for 31 species of ladybird beetles (Coleoptera: Coccinellidae)

This study provides C-value (haploid nuclear DNA content) estimates for 31 species of ladybird beetles (representing 6 subfamilies and 8 tribes), the first such data for the family Coccinellidae. Despite their unparalleled diversity, the Coleoptera have been very poorly studied in terms of genome size variation, such that even this relatively modest sample of species makes the Coccinellidae the third best studied family of beetles, behind the Tenebrionidae and Chrysomelidae. The present study provides a comparison of patterns of genome size variation with these two relatively well-studied families. No correlation was found between genome size and body size in the ladybirds, in contrast to some other invertebrate groups but in keeping with findings for other beetle families. However, there is some indication that developmental time and/or feeding ecology is related to genome size in this group. Some phylogenetic patterns and possible associations with subgenomic features are also discussed.     

Record of the invasive alien ladybird Harmonia axyridis (Coleoptera, Coccinellidae) from Kenya

The biological control agent and alien invasive ladybird Harmonia axyridis (Pallas, 1773) was recorded for the first time in Kenya, and in equatorial Africa, in 2010.     

瓢虫性选择行为及进化机制研究

昆虫性选择行为一直是行为生物学家和进化生物学家所关注的热点。早期对瓢虫性选择行为研究主要集中在非随机性交配模式,随着研究的深入,近些年对瓢虫性选择行为研究取得了许多新成果,包括多次交配的行为机制、性选择的识别机制、精子传送及竞争等。为全面地了解瓢虫性选择行为研究现状,本文总结了瓢虫非随机性交配模式,综述了近十余年对瓢虫性行为及进化的研究成果,同时对瓢虫性选择行为未来的研究方向进行了展望。     

Ecological correlates of local extinction and colonisation in the British ladybird beetles (Coleoptera: Coccinellidae)

Five main drivers of population declines have been identified: climate change, habitat degradation, invasive alien species (IAS), overexploitation and pollution. Each of these drivers interacts with the others, and also with the intrinsic traits of individual species, to determine species’ distribution and range dynamics. We explored the relative importance of life-history and resource-use traits, climate, habitat, and the IAS Harmonia axyridis in driving local extinction and colonisation dynamics across 25 ladybird species (Coleoptera: Coccinellidae).Species were classified as continually present, continually absent, extinct, or colonising in each of 4,642 1-km² grid squares. The spatial distribution of local extinction and colonisation events (in the grid squares) across all species’ ranges were related to ecological traits, overlap with H. axyridis, climate, and habitat factors within generalised linear models (GLMs). GLMs were also used to relate species’ traits, range characteristics, and niche overlap with H. axyridis to extinction and colonisation rates summarised at the species level. Bayesian model averaging was used to account for model uncertainty, and produce reduced sets of models which were well-supported by data. Species with a high degree of niche overlap with H. axyridis suffered higher extinction rates in both analyses, while at the spatial scale extinctions were more likely and colonisations less likely in areas with a high proportion of urban land cover. In the spatial analysis, polymorphic species with large range sizes were more likely to colonise and less likely to go extinct, and sunny grid squares were more likely to be colonised. Large, multivoltine species and rainy grid squares were less likely to colonise or be colonised. In conclusion for ladybirds, extinction and colonisation dynamics are influenced by several factors. The only factor that both increased the local extinction likelihood and reduced colonisation likelihood was urban land cover, while ecological overlap with H. axyridis greatly increased extinction rates. Continued spread of H. axyridis is likely to adversely affect native species and urban areas may be particularly vulnerable.     

三种瓢虫集团内捕食卵对初龄幼虫生长发育的影响

种内和种间捕食卵现象常见于对食蚜瓢虫的研究报道中。源自亚洲、入侵北美和欧洲的异色瓢虫(Harmonia axyridis)由于卵含有生物碱而被认为可能是威胁本土瓢虫生存的原因,但该推测只有在明确异色瓢虫在原产地是否对本土瓢虫具有类似负面影响后,才能确证。通过饲喂实验,旨在明确异色瓢虫在原产地是否对本土七星瓢虫(Coccinella septempunctata)和龟纹瓢虫(Propylea japonica)具有食卵负面作用,为揭示异色瓢虫入侵机理提供依据。实验中用蚜虫(对照)和3种瓢虫卵对瓢虫初孵1龄幼虫进行饲喂处理,观察幼虫生长发育指标。对瓢虫初龄幼虫存活表现的分析结果表明:(1)龟纹瓢虫初孵幼虫取食同种卵和七星瓢虫卵后存活至2龄的比率均高于取食异色瓢虫卵的近7倍;(2)七星瓢虫幼虫取食异色瓢虫卵未存活到2龄,但取食其他瓢虫卵和蚜虫后85%以上个体存活至2龄;(3)异色瓢虫初孵幼虫取食同种和其他2种瓢虫卵后有90%以上存活至2龄。龟纹瓢虫和七星瓢虫1龄幼虫取食异种卵比同种卵后的发育历期显著延长,但异色瓢虫并未表现出差异。龟纹瓢虫和异色瓢虫1龄幼虫取食同种和异种卵后的体增重相同,但七星瓢虫取食龟纹瓢虫卵后体增重比取食同种卵减小。研究结果说明,异色瓢虫卵对七星瓢虫和龟纹瓢虫初孵幼虫的生存具有负面影响,反之不然。所以,取食异色瓢虫卵本身并非是导致北美和欧洲本土瓢虫数量减少的原因。     

Extreme length and length variation in the first ribosomal internal transcribed spacer of ladybird beetles (Coleoptera: Coccinellidae)

DNA sequences of the first ribosomal internal transcribed spacer (ITS1) were isolated from 10 ladybird beetle species (Coleoptera: Coccinellidae) representing four subfamilies (Coccinellinae, Chilocorinae, Scymninae, and Coccidulinae). The spacers ranged in length from 791 to 2,572 bp, thereby including one of the longest ITS1s and exhibiting one of the most extreme cases of ITS1 size variation in eukaryotes recorded to date. The causes of length variation were therefore analyzed. Almost no putatively homologous sequence similarities were identified for the taxa included. The only exception was for the subfamily Coccinellinae, which yielded sequence similarities in six regions of approximately 550 nucleotide positions, primarily at the 5' and 3' ends of ITS1. The majority of differences in ITS1 length between taxa could be attributed to the presence of repetitive elements with comparatively long repeat units. Repetition arose several times independently and was confined to the middle of the spacer which, in contrast to the 5' and 3' ends, had not been inferred in previous studies to be subject to functional constraints. These elements were characterized by high rates of evolutionary change, most likely as a result of high substitution rates in combination with inefficient homogenization across repeats. The repeated origin and subsequent divergence of "long" repetitive elements should thus be assumed to be an important factor in the evolution of coccinellid ITS1.     

In vitro production of adaline and coccinelline,two defensive alkaloids from ladybird beetles (Coleoptera: Coccinellidae)

In vitro experiments using [1-(14)C] and [2-(14)C]acetate were devised to study the biosynthesis of the defensive coccinellid alkaloids adaline and coccinelline in Adalia 2-punctata and Coccinella 7-punctata, respectively. The labelled alkaloids obtained in these experiments had a specific activity about ten times higher than that of the samples obtained in feeding experiments. This in vitro assay has enabled us to demonstrate that these two alkaloids are most likely biosynthesised through a fatty acid rather than a polyketide pathway, that glutamine is the preferred source of the nitrogen atom and that alkaloid biosynthesis takes place in the insect fat body.     

Evolutionary significance of promiscuity in an aphidophagous ladybird, Propylea dissecta (Coleoptera: Coccinellidae)

The short- and long-term consequences of monogamy and promiscuity in an aphidophagous ladybird, Propylea dissecta (Mulsant) in terms of reproductive performance and offspring fitness were investigated. Promiscuous females were more fecund and laid more viable eggs than monogamous ones. Amongst promiscuous females, those mated with multiple males (freedom for mate choice) had significantly higher reproductive output than those mated daily with a new unmated male. The increased reproductive output of females mated with an unmated male each day in comparison to monogamous ones might possibly be a result of their increased ejaculate size. The further increase in reproductive output, when a female was allowed to choose a mate, may be because of repeated preferential mating with the fitter males. Results on long-term consequences of promiscuity revealed faster development and increased survival in offspring of promiscuous females at 25, 27 and 30 degrees C. The percent adult emergence was more strongly influenced than development by the mating regime. There were no statistically significant differences in survival of offspring of promiscuous females (mate choice) at the three temperatures, while the percent survival of offspring of monogamous females differed significantly. Offspring of promiscuous females (mate choice) were fittest in terms of development and survival, which indicates their probable better genetic constitution and subsequently that of chosen mates. Thus, promiscuity has both short- and long-term benefits and is advantageous to ladybirds. The presence of long-term benefits in terms of fitter offspring probably indicates the evolutionary rationale behind the prevalence of promiscuity in ladybirds.     

伊朗寄生蓟马的侧姬小蜂属Ceranisus种类记述(膜翅目:姬小蜂科:凹面姬小蜂亚科)(英文)

侧姬小蜂属Ceranisus Walker,1841隶属膜翅目Hymenoptera、小蜂总科Chalcidoidea、姬小蜂科Eulophidae、凹面姬小蜂亚科Entedoninae,是蓟马幼虫重要的寄生蜂,迄今世界上已知16种。该属颜面凹陷,额V字形,槽沟伸达中单眼水平,侧单眼后部有1条完整的沟横穿颅顶,颚眼沟一般不分叉,上颚退化;雌性触角索节2分节,棒节2或3分节;盾纵沟不明显,一般中胸盾片中叶着生2对刚毛,小盾片1对刚毛;前翅缘毛短于翅宽,后翅末端尖锐;腹柄短,宽明显大于(或至少等于)长等特征明显,较易识别。本文首次报道该属伊朗的3种类:月侧姬小蜂Ceranisus menes(Walker,1839),平侧姬小蜂C.planitianus Erds,1966和爱侧姬小蜂C.amanosus Doganlar et al.,2009,这也是该属在伊朗的首次记录。并对近缘种爱侧姬小蜂C.amanosus Doganlar et al.,2009和乌侧姬小蜂C.udnamtak Tryapitsin,2005的鉴别进行了简要讨论。     

海南岛食植瓢虫属一新种和二种新记录（鞘翅目：瓢虫科）

论述了采自海南岛的食植瓢虫属Epilachna一新种和二种中国新记录：拟球端食植瓢虫E. paraglobiera 新种; 同亨食植瓢虫E. donghoiensis Hoang, 1978中国新记录; 戈特克食植瓢虫E. gokteika Kapur, 1961中国新记录。     

Expansion of the geographical distribution of an exotic ladybird beetle, Adalia bipunctata (Coleoptera: Coccinellidae), and its interspecific relationships with native ladybird beetles in Japan

The first record of the exotic ladybird beetle, Adalia bipunctata (Coleoptera; Coccinellidae), in Japan was in 1993 at Osaka Nanko Central Park. Since that time, studies on the life history and geographical distribution of A. bipunctata have been ongoing, and its establishment in the Osaka Nanko area has been confirmed. A. bipunctata is a predacious ladybird beetle and a member of a guild that overlaps in habitat and prey with that of native ladybird beetles such as Harmonia axyridis and Menochilas sexmaculatus. We investigated the distribution of A. bipunctata and its interspecific relationships with native predacious ladybird beetles. In some areas, A. bipunctata was dominant in interspecific relationships with native ladybird beetles. For the first 10 years after A. bipunctata was discovered, it occurred only in the Osaka Nanko area, but the present geographical distribution indicates that it has expanded its range. Though the population density of this species was highest at the area recorded first, and tended to decrease in inverse proportion to the distance from Osaka Nanko Central Park, a satellite occurrence was observed in a remote area. The numbers of aphid and tree species (leaf shelter for aestivation and over-wintering) utilized by A. bipunctata have recently increased. Such increases will cause the rate of distribution of A. bipunctata to accelerate. Interspecific competition between H. axyridis and A. bipunctata, which occurs earlier than H. axyridis, may be avoided by desynchronization of the occurrence seasons, and another common predacious ladybird beetle, Coccinella septempunctata, may escape interspecific competition by habitat segregation.     

Phylogeny and evolution of phytophagous ladybird beetles (Coleoptera: Coccinellidae: Epilachnini), with recognition of new genera

Phytophagous ladybird beetles of the tribe Epilachnini are a cosmopolitan, species‐rich group of significant economic importance as pests of agricultural crops. The tribe is well characterized morphologically and clearly monophyletic, but very little is known about its internal phylogenetic relationships and their genus‐level taxonomy. In order to infer the evolutionary history of Epilachnini, test its monophyly and provide a phylogeny‐based classification, we assembled a comprehensive dataset, consisting of four DNA markers (18S and 28S rRNA and 16S, COI mtDNA) and a matrix of 104 morphological characters for 153 species of Epilachnini representing all previously recognised genera, ～11% of the known species, and 14 outgroup taxa. Molecular, morphological and combined datasets were analysed using maximum likelihood, parsimony and Bayesian inference. Bayes factors and Approximately Unbiased tests (AU) were used to compare alternative phylogenetic hypotheses of unconstrained and backbone‐constrained analysis. Only 14 of the 25 included genera were recovered monophyletic, as originally defined. Afidentula Kapur, Afidenta Dieke, Afissula Kapur, Epilachna Chevrolat, Henosepilachna Li Toxotoma Weise and Mada Mulsant are shown to be poly‐ or paraphyletic; Chnootriba Chevrolat, Subafissa Bielawski, Lalokia Szawaryn & Tomaszewska and Papuaepilachna Szawaryn & Tomaszewska form monophyletic groups within larger clades of genus level. All of these genera are redefined here. The two largest genera of Epilachnini, Epilachna Chevrolat and Henosepilachna Li were represented by multiple monophyletic clades, which we described as new genera: Chazeauiana Tomaszewska & Szawaryn gen.n. ; Diekeana Tomaszewska & Szawaryn gen.n .; Fuerschia Tomaszewska & Szawaryn gen.n. and Ryszardia Tomaszewska & Szawaryn gen.n . The following new synonyms are proposed: Afissa Dieke (=Afissula Kapur); Henosepilachna Li in Li & Cook (=Subafissa Bielawski); Papuaepilachna Szawaryn & Tomaszewska (=Lalokia Szawaryn & Tomaszewska). This published work has been registered in ZooBank, http://zoobank.org/urn:lsid:zoobank.org:pub:440E7FA4‐C859‐47E0‐8335‐30D478CBA8FA .     

Attack strategy of ladybird beetles (Coccinellidae): factors shaping their numerical response

Summary Temporal changes in aphid abundance pose a considerable challenge to ovipositing aphidophagous ladybirds, as in order to maximize their fitness they need to synchronize their reproduction with the early development of aphid populations. Field census data and laboratory experiments were used to determine how ovipositing females of the two-spot ladybird, Adalia bipunctata (L.), assess whether an aphid population is suitable for exploitation. In the field, two-spot ladybirds usually laid eggs well before aphid populations peaked in abundance. In the laboratory they showed a marked reduction in their reproductive numerical response in the presence of larvae of their own species but not of other aphidophagous ladybirds. At the highest aphid density this was not a consequence of competition for food between larvae and ovipositing females. In the presence of conspecific larvae gravid females were very active and as a consequence more likely to leave an area, and when confined with other conspecific females or larvae laid fewer eggs and later than females kept on their own. The extent of the inhibition of egg laying is negatively correlated with the rate of encounter with larvae. Thus it is proposed that gravid females appear mainly to use the presence of conspecific larvae to assess the potential of an aphid colony for supporting the development of their offspring.     

Glutathione S-transferase isoenzymes in the two-spot ladybird, Adalia bipunctata (Coleoptera: Coccinellidae)

Isoenzymes of glutathione S-transferase (GST) in adult Adalia bipunctata, an aphidophagous predator, were studied. Cytosolic GST activity was studied in each beetle developmental stage. The highest activities towards both 1-chloro-2,4-dinitrobenzene (CDNB) and 2,4-dinitro-1-iodobenzene (DNIB) occurred in adults. The enzyme distribution was investigated in adults. While most of the enzymatic activity was found in the abdomen (40-50 and 34-63% respectively) using several concentrations of both CDNB and DNIB, significant differences were observed for the head and the thorax depending on the substrate. Activities were more abundant in the thorax with DNIB (37-47%) compared to the 13-19% obtained with CDNB. Some GST activity was also detected in the elytra. GSTs were purified by epoxy-activated Sepharose 6B affinity chromatography and applied to an HPLC column to determine the native molecular weight (69 kDa). Three isoenzymes were separated by chromatofocusing at pH ranges 7-4. Three bands with molecular mass from 23 to 26 kDa were visualised on SDS-PAGE. Their isoelectric points were 6.66, 6.36, and 6.21. The substrate specificities and the kinetic parameters (Vm and Km) of the isoenzymes showed large differences depending on the isoenzyme. Arch.     

Fine structure of the ladybird spermatozoa (Insecta,Coleoptera, Coccinellidae)

The sperm structure of several ladybird species belonging to different subfamilies of Coccinellidae was studied. Three main sperm types were clearly recognized, and were characterized by differences in acrosomal length, the presence of a dense coat around the acrosome, the length of the basal body, the amount of the centriole adjunct material, and the diameter of the mitochondrial derivatives. However, the whole group shares a pattern of the posterior sperm region uncommon for insects, in which the axoneme and other flagellar components are running parallel with the nucleus. As a general conclusion, this study has revealed an inconsistency between the sperm structure and the systematics of the group, indicating that the generic concepts within the group do not reflect a natural classification, a statement also shared by molecular studies.     

Susceptibility of lady beetles (Coleoptera: Coccinellidae) to entomopathogenic nematodes

We investigated differential susceptibility of lady beetles to entomopathogenic nematodes, for two reasons: (1) to estimate potential nontarget effects on natural lady beetle populations, (2) to compare the susceptibility of exotic versus native lady beetle species. We hypothesize that successful establishment of some exotically introduced arthropods may be due, in part, to a lower susceptibility relative to competing native species. In laboratory studies, we compared the pathogenicity, virulence, and reproductive capacity of Heterorhabditis bacteriophora and Steinernema carpocapsae among two native (Coleomegilla maculata and Olla v-nigrum) and two successfully established exotic (Harmonia axyridis and Coccinella septempunctata) lady beetles, and a known susceptible lepidopteran host, Agrotis ipsilon. After 1 and 2 days of exposure to either nematode species, mortality of A. ipsilon was higher than in all lady beetles. Thus, we predict that nematode field applications would have significantly less impact on lady beetle populations than on a susceptible target pest. Additionally, the impact of soil-applied nematodes may be lower on lady beetles than on soil-dwelling hosts because the former spends relatively less time on the soil. Exotic lady beetles were less susceptible to nematode infection than native species. Reproductive capacity data also indicated lower host suitability in H. axyridis, but not in C. septempunctata. Overall, the hypothesis that low susceptibility to pathogens in certain exotic lady beetles may have contributed to competitive establishment was supported (especially for H. axyridis). Additional studies incorporating different hosts and pathogens from various geographic locations will be required to further address the hypothesis.     

Phylogeny, classification and evolution of ladybird beetles (Coleoptera: Coccinellidae) based on simultaneous analysis of molecular and morphological data

Ladybird beetles (family Coccinellidae) are a species-rich, ecologically diverse group of substantial agricultural significance, yet have been consistently problematic to classify, with evolutionary relationships poorly understood. In order to identify major clades within Coccinellidae, evaluate the current classification system, and identify likely drivers of diversification in this polyphagous group, we conducted the first simultaneous Bayesian analysis of morphological and multi-locus molecular data for any beetle family. Addition of morphological data significantly improved phylogenetic resolution and support for early diverging lineages, thereby better resolving evolutionary relationships than either data type alone. On the basis of these results, we formally recognize the subfamilies Microweisinae and Coccinellinae sensu?lipiński (2007). No significant support was found for the subfamilies Coccidulinae, Scymninae, Sticholotidinae, or Ortaliinae. Our phylogenetic results suggest that the evolutionary success of Coccinellidae is in large part attributable to the exploitation of ant-tended sternorrhynchan insects as a food source, enabled by the key innovation of unusual defense mechanisms in larvae.     

食植瓢虫属Epilachna中国新记录种及两种幼虫的形态记述(鞘翅目:瓢虫科)

记述食植瓢虫属 Epilachna Chevrolat3种中国新记录种及其 2种幼虫形态 ,编制了该属中国已知幼虫种类检索表。新记录种和幼虫标本均保存于南京农业大学昆虫标本室。