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骆驼蓬的组织培养及植株再生 总被引:1,自引:0,他引:1
以骆驼蓬(Peganum harmala L)无菌苗下胚轴切段为材料,在不同的培养基上进行愈伤组织的诱导,发现在MS基本培养基附加2.0mg/L 2,4—D、0.5mg/L 6—BA和3%蔗糖时,可100%的诱导出愈伤组织。愈伤组织在附加2.0mg/L 6—BA、0.5mg/L NAA、500mg/L CH和3%蔗糖的MS培养基上诱导出丛生芽,进而发育成苗,苗的分化频率在30%左右。分化苗或其茎切断在附加0.2mg/L IBA、0.2mg/L NAA和3%蔗糖的l/2MS培养基上出现根的分化,分化频率在90%以上。再生植株经炼苗后移栽成活,成活率在80%以上。 相似文献
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地黄组织培养及植株再生的研究 总被引:14,自引:2,他引:14
以地黄根茎所获无菌苗为材料,对其愈伤组织诱导、分化和再生植株的获取进行了初步研究。结果表明:取叶片、茎段、叶柄进行愈伤组织诱导,筛选出最适培养基为MS附加2,4-D0.5mg/L、BA1.0mg/L,愈伤组织诱导率可达100%。将叶片接种在分化培养基中,诱导不定芽,其最适分化培养基为MS附加BA 3mg/L、NAA 0.1mg/L,分化率为77.5%。试管苗在改良的MS(大量与微量元素、铁盐和有机物质各1/2)附加NAA 0.05mg/L的培养基上,经过15~20d培养,生根率可达100%。 相似文献
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目的:建立山茱萸的组织培养及植株再生体系。方法:分别以山茱萸的叶片、花柄和花托为材料,进行山茱萸不同外植体的离体培养研究,筛选最佳培养基组成。结果:适宜山茱萸叶片愈伤组织诱导的培养基组合为1/2MS,附加BA2.0mg/L、IBA0,5—1.0mg/L;适宜山茱萸花柄、花托愈伤组织诱导的培养基组合为1/2MS,附加BA1.0mg/L、2,4-D0.5mg/L;在1/2MS附加BA2.0mg/L、IBA0.05mg/L的培养基上,可诱导不定芽的产生;1/2MS附加IBA2.0mg/L的培养基有利于山茱萸试管苗生根。讨论:山茱萸的花托是进行组织培养的最适外植体,白色或翠绿色、结构致密的愈伤组织较易分化产生不定芽。 相似文献
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大果良种沙棘愈伤组织诱导及植株再生的研究 总被引:18,自引:1,他引:17
大果良种沙棘的幼嫩茎尖,茎段外植体接种在MS,1/2MS附加不同浓度配比的IAA,IBA,BA,NAA培养基上可诱导茎尖及腋芽生长,将诱导产生的无性系芽接种在MS或1/2MS附加BA0.3-0.5mg/L,NAA0.05mg/L的培养基上可形成丛生芽,同时在小叶片和嫩茎上诱导产生愈伤组织,继续培养愈伤组织表面形成大量的绿色突起,进一步分化成不定芽,在相同培养基上,不定芽上可直接产生不定芽,从而形成多达数百个的不定芽族,不定芽长至3cm时切下转至1/2MS附加IAA或IBA 0.2mg/L的培养基上可生根而形成完整 的再生植株。 相似文献
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贯叶金丝桃组织培养的研究 总被引:1,自引:0,他引:1
分别以甘肃天水贯叶金丝桃的幼根、幼茎、幼叶为外植体.在1/2MS培养基上附加各类激素,进行贯叶金丝桃的组培实验。研究发现各外植体的增殖速率由高到低分别为幼茎、幼根、幼叶,且得到贯叶金丝桃组培各阶段的最佳培养基成分。诱导愈伤组织的培养基为1/2MS 1.3~1.6mg/L BA 0.2mg/L NAA;培养基1/2MS 1.3~1.6mg/L BA 0.15mg/L NAA有利于不定芽的形成;诱导不定根的培养基为l/2MS IBA0.5~O.8mg/L 蔗糖2.0%。向1/2MS培养基中添加不同的生长素(IAA,IBA,NAA,2.4-D).在不同浓度梯度的培养基上进行诱导贯叶金丝桃的愈伤组织及不定根的试验,结果表明:生长素IAA,IBA既可诱导愈伤组织,又可以诱导不定根的产生。生长素NAA,2,4-D可诱导产生愈伤组织,但对不定根的诱导作用较差。 相似文献
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叶子花的组织培养与微繁技术研究 总被引:11,自引:0,他引:11
叶子花茎尖、茎段接种在MS附加不同浓度组合的6-BA、IAA、IBA、NAA培养基上可诱导茎尖及腋芽的生长而获得无性系芽,无性系芽茎尖和茎段在MS附加6-BA0.5mg/L,NAA0.05mg/L进行继代培养,一般不形成丛生芽,以茎尖生长和腋芽萌生增殖,增殖系数为2.73;高度大于3cm的增殖芽在1/2 MS附加IAA lmg/L、IBA lmg/L、NAA0.2mg/L培养基上生根率为80.5%,生根苗用“二步法”移栽成活率在97%以上。无性系芽的幼叶、茎段在MS附加6-BA和NAA、2,4-D培养基上能高频产生愈伤组织,但愈伤组织在所试验的所有培养基上均无不定芽或胚状体的分化。 相似文献
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探讨了植物生长调节剂、外植体等因子对费尔干猪毛菜(Salsola fergartica Drob.)愈伤组织诱导、分化与植株再生的影响。结果表明:2,4-D与6-BA组合使用时,在一定浓度范围内均有愈伤组织产生,最佳的诱导培养基为MS+2,4-D2.0mg/L+6-BA0.2mg/L,下胚轴为诱导愈伤组织的最佳材料;愈伤组织继代培养较好的培养基为MS+NAA0.1mg/L+6-BA1.0mg/L;愈伤组织不定芽分化培养基为MS+6-BA0.5mg/L+NAA0.05mg/L;根分化的培养基为1/2MS+IBA0.2mg/L,且生根率可达72%。以带柄子叶为外植体,诱导丛生芽的最佳分化培养基为MS+6-BA1.0mg/L,再生频率可达90.74%。 相似文献
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太白米组织培养的研究 总被引:2,自引:0,他引:2
太白米地下鳞茎在MS附加不同浓度KT、BA、IAA、NAA、2,4-D的培养基上,可诱导产生愈伤组织,其中在MS附加NAA 0.5mg/L,KT0.1mg/L的培养基上愈伤组织诱导率最高,可达65%,且生长快;培养在MS附加2,4-D 1.0mg/L,KT 0.1mg/L培养基上的鳞茎可经不定根直接发育成新的鳞茎,由此建立了太白米的鳞茎再生体系,鳞茎再生率达2.17倍。 相似文献
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黄山药愈伤组织诱导与分化 总被引:2,自引:1,他引:1
采用黄山药野生植株作为外植体,试验了不同激素处理对黄山药愈伤组织的诱导、分化影响,结果表明:不同的外植体的诱导率差别较大,叶片的诱导率最高,最高达到85.7%,茎段的诱导率较低,平均诱导率仅10%左右。以叶片作为外植体诱导愈伤组织的最佳培养基配方为MS 2,4-D2.0mg/L 6-BA2.5mg/L;愈伤组织分化生芽的最佳配方为MS BA1.0mg/L NAA0.5mg/L 蔗糖2% pH6.4;愈伤组织分化生根的最佳配方诱MS BA1.0mg/L NAA0.1mg/L 蔗糖3% pH6.80。 相似文献
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In experiments on Black Sea skates (Raja clavata), the potential of the receptor epithelium of the ampullae of Lorenzini and spike activity of single nerve fibers connected to them were investigated during electrical and temperature stimulation. Usually the potential within the canal was between 0 and –2 mV, and the input resistance of the ampulla 250–400 k. Heating of the region of the receptor epithelium was accompanied by a negative wave of potential, an increase in input resistance, and inhibition of spike activity. With worsening of the animal's condition the transepithelial potential became positive (up to +10 mV) but the input resistance of the ampulla during stimulation with a positive current was nonlinear in some cases: a regenerative spike of positive polarity appeared in the channel. During heating, the spike response was sometimes reversed in sign. It is suggested that fluctuations of the transepithelial potential and spike responses to temperature stimulation reflect changes in the potential difference on the basal membrane of the receptor cells, which is described by a relationship of the Nernst's or Goldman's equation type.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. I. M. Sechenov, Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Pacific Institute of Oceanology, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Neirofiziologiya, Vol. 12, No. 1, pp. 67–74, January–February, 1980. 相似文献
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N. P. Vesselkin Yu. V. Natochin 《Journal of Evolutionary Biochemistry and Physiology》2010,46(6):592-603
Evolution of living organisms is closely connected with evolution of structure of the system of regulations and its mechanisms.
The functional ground of regulations is chemical signalization. As early as in unicellular organisms there is a set of signal
mechanisms providing their life activity and orientation in space and time. Subsequent evolution of ways of chemical signalization
followed the way of development of delivery pathways of chemical signal and development of mechanisms of its regulation. The
mechanism of chemical regulation of the signal interaction is discussed by the example of the specialized system of transduction
of signal from neuron to neuron, of effect of hormone on the epithelial cell and modulation of this effect. These mechanisms
are considered as the most important ways of the fine and precise adaptation of chemical signalization underlying functioning
of physiological systems and organs of the living organism 相似文献
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