共查询到20条相似文献,搜索用时 15 毫秒
1.
Y. Tsumura K. Yoshimura N. Tomaru K. Ohba 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,91(8):1222-1236
We investigated the molecular phylogeny of conifers using restriction endonuclease fragment length polymorphism of six polymerase chain reaction-amplified chloroplast genes — frxC, rbcL, psbA, psbD, trnK, and 16S. We detected 227 total site changes among species, representing 23, 26, 38, 48, 67, and 25 site changes in frxC, psbA, psbD, rbcL, trnK and 16S, respectively. The mean nucleotide substitution was 10.75% (SD 0.573) among species in five families. Forty maximally parsimonious trees were obtained using the Wagner parsimony method, and a 50% majority-rule consensus tree was obtained from them. Data analysis produced similar basic patterns when both the Wagner parsimony and the neighbor-joining methods were applied, and the main lineages were clearly separated. Taxaceae and Cephalotaxaceae species were used as the out-groups when applying Wagner parsimony methods. With the Wagner method, the consistency index was 0.510, the retention index was 0.879, and tree length was 435 steps. Our results indicated that Cupressaceae and Taxodiaceae are closely related families and that Sciadopitys verticillata is the basal lineage of Cupressaceae and Taxodiaceae. The neighbor-joining tree is similar to the 50% majority-rule consensus of the 40 Wagner parsimony trees except for the position of Keteleeria daversifolia, the Picea and Cedrus group, and the divergence within Cupressaceae. 相似文献
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S. NANAMI S. IKEDA N. TANI S. TAN B. DIWAY K. HARADA Y. TSUMURA A. ITOH T. YAMAKURA 《Molecular ecology resources》2007,7(4):623-625
Eight polymorphic microsatellite markers were developed in Dryobalanops aromatica, an emergent tree in tropical rain forests in Southeast Asia, using an enriched library method. For the assessment of microsatellite variation, 36 individuals from a natural population were analysed. The number of alleles per locus ranged from three to 16, with observed heterozygosity of 0.056–0.833 and expected heterozygosity of 0.054–0.882. These microsatellite markers will be useful for studies of population genetics, reproductive ecology and regeneration dynamics of D. aromatica. 相似文献
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In order to construct a molecular phylogeny of Indonesian Dipterocarpoideae (Dipterocarpaceae), PCR-RFLP of the chloroplast
regions rbcL, petB, psbA, psaA, and trnL-F was performed with seven restriction enzymes in 129 samples including 58 species from nine genera. In the strict consensus
tree with Monotes kerstingii as outgroup Indonesian Dipterocarpaceae were divided into two major clades. One clade (bootstrap value=71) consisted of Upuna, Cotylelobium, Anisoptera, Vatica, Dipterocarpus (tribe Dipterocarpeae, bootstrap value=83) and Dryobalanops (tribe Shoreae, bootstrap value=99) in a basal position. The second clade consisted of Hopea, Parashorea, and Shorea (tribe Shoreae) with 95% bootstrap support. Tribe Dipterocarpeae is monophyletic, tribe Shoreae is polyphyletic since Dryobalanops is sister to tribe Dipterocarpeae. In the neighbour-joining tree the sister group position of Dryobalanops to tribe Dipterocarpeae is not supported by the bootstrap analysis. Alternatively, we used Upuna borneensis as outgroup. The effect of outgroup selection on tree topology, taxonomic classification and the interpretation of character
evolution is discussed. 相似文献
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The classification of taxa within Collembola (Springtails, Hexapoda) has been controversial. In this study, we combined complete 18S rRNA gene with partial 28S rRNA gene (D7-D10) sequences to investigate the phylogeny of Collembola. About 2500 aligned sites of thirty species representing 29 genera from14 families of Collembola were analyzed, including one species of Neelipleona from which no sequence has been reported previously.The phylogenetic trees were obtained by different methods (maximum parsimony, maximum likelihood, and Bayesian analysis). Our results supported the monophyly of two of the four taxonomic groups of Collembola summarized by Deharveng [Deharveng, L., 2004. Recent advances in Collembola systematics. Pedobiologia 48, 415–433.], namely of Poduromorpha and of Symphypleona. Within Poduromorpha, Neanuridae was monophyletic with high support, but Hypogastruridae was not. Entomobryomorpha was paraphyletic, as the Tomoceroidea (Tomoceridae and Oncopoduridae) was found to be apart from the other entomobryomorphs. In the latter Isotomoidea and Entomobryoidea joined into a group with moderate support. Within Symphypleona, the phylogenetic relationship [(Sminthuridae + Bourletiellidae) + Sminthurididae] was consistent with traditional morphological studies. Neelipleona grouped with Symphypleona in all trees, with moderate support in the ML and Bayesian analyses. 相似文献
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Microbial control in Southeast Asia 总被引:1,自引:0,他引:1
Skovmand O 《Journal of invertebrate pathology》2007,95(3):168-174
Beginning in the 1980s, concerns about the deleterious effects of synthetic pesticides have driven a significant Southeast Asian research and development effort directed towards alternative pest control strategies, including the use of microbial control agents. Despite this effort, use of microbial control agents has grown slowly in the region. This is the result of an interplay between internal factors such as economics, national research programs, farmer education, manufacturing capabilities and regulatory frameworks, and external factors such as the influence of neighboring countries (particularly China), the availability of competitive pest control products, import regulations on pesticide residues and the activities of donor agencies. The role of these factors in providing both incentives and barriers to the adoption of microbial control are explored, and examples of promising projects are examined as a means of pointing the way forward towards increased progress in the future. 相似文献
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洪平杏(Armeniaca hongpingensis C. L. Li)是杏属的一个狭域分布种,基于形态观察被推测为杏(A.vulgaris Lam.)和梅(A. mume Sieb.)的天然杂交种,但目前尚无该种与杏、梅亲缘关系的分子系统学研究。本文以洪平杏的成株和实生苗以及包括杏、梅在内的6种(含1变种)杏属植物为研究材料,分别采用核基因(ITS和SBEI)和叶绿体基因(mat K和ycf1b)序列构建系统发育树,并采用mat K、ycf1b和SBEI基因序列构建单倍型网络图,探讨该物种与杏、梅及杏梅(A. mume Sieb. var. bungo Makino)之间的亲缘关系。基于核基因和叶绿体基因序列分别构建的系统发育树均显示,洪平杏的成株及其全部实生苗个体单独聚为一支,且具有较高的支持率(分别为99/79、71/81),独立于杏属其他种之外。而基于核基因ITS序列的系统发育分析结果表明,洪平杏除极少数成株与杏、杏梅聚为一支外,其余所有成株与实生苗聚为2大支(支持率分别为0.82和0.97),而没有克隆的与梅聚在一起。单倍型分析结果表明,该物种的成株与实生苗在SBEI和ycf1b基因序列中均未检测到杏或梅的单倍型,仅有少数(2/9)的实生苗个体在叶绿体mat K基因序列中检测到杏的单倍型。研究结果不支持将洪平杏定为杏和梅的天然杂交种的观点,推测洪平杏应为一个独立的物种,与杏之间的亲缘关系更近并且存在可检测到的基因流。 相似文献
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东南亚稻种资源收集与鉴定评价 总被引:1,自引:0,他引:1
通过实地考察收集等途径,从东南亚国家引进稻种资源662份,其中野生稻120份,栽培稻542份。对栽培稻资源进行隔离检疫、繁殖以及性状鉴定,获得足够种子并构建引进东南亚栽培稻资源数据库。从栽培稻种质中鉴定发掘出抗褐飞虱材料58份,抗稻瘟病材料9份,抗白叶枯病材料11份,耐盐材料12份。引进的稻种资源拓宽了我国水稻遗传基础,丰富了我国的稻种资源基因库,将在水稻育种及相关研究中发挥积极的作用。 相似文献
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John Mo 《Ocean Development & International Law》2013,44(3-4):343-358
Modern maritime piracy has become one of the major threats to safety at sea. How to combat maritime piracy in Southeast Asia effectively is the major concern of this contribution. It is argued that some form of government cooperation involving most of the governments of the Southeast Asian region is the best way to combat maritime piracy, although it is not an easy task due to various political, economical, and historical reasons. A unilateral and expedient arrangement by one or a few governments to combat maritime piracy may be convenient but may also encounter resistance or raise suspicion from other governments. 相似文献
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Policies,Political-Economy,and Swidden in Southeast Asia 总被引:7,自引:5,他引:2
Fox Jefferson Fujita Yayoi Ngidang Dimbab Peluso Nancy Potter Lesley Sakuntaladewi Niken Sturgeon Janet Thomas David 《Human ecology: an interdisciplinary journal》2009,37(3):305-322
For centuries swidden was an important farming practice found across the girth of Southeast Asia. Today, however, these systems
are changing and sometimes disappearing at a pace never before experienced. In order to explain the demise or transitioning
of swidden we need to understand the rapid and massive changes that have and are occurring in the political and economic environment
in which these farmers operate. Swidden farming has always been characterized by change, but since the onset of modern independent
nation states, governments and markets in Southeast Asia have transformed the terms of swiddeners’ everyday lives to a degree
that is significantly different from that ever experienced before. In this paper we identified six factors that have contributed
to the demise or transformation of swidden systems, and support these arguments with examples from China (Xishuangbanna),
Laos, Thailand, Malaysia, and Indonesia. These trends include classifying swiddeners as ethnic minorities within nation-states,
dividing the landscape into forest and permanent agriculture, expansion of forest departments and the rise of conservation,
resettlement, privatization and commoditization of land and land-based production, and expansion of market infrastructure
and the promotion of industrial agriculture. In addition we note a growing trend toward a transition from rural to urban livelihoods
and expanding urban-labor markets.
相似文献
David ThomasEmail: |
12.
M. Parani M. Lakshmi P. Senthilkumar Nivedita Ram A. Parida 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(4):617-625
DNA from pooled leaf samples of 11 true major mangrove, three true minor mangrove, two mangrove associate, two mangrove parasite,
three terrestrial and one cultivated species were isolated for the present study. In total, 198 random amplified polymorphic
DNAs (RAPDs) and 180 restriction fragment length polymorphism (RFLP) loci were scored by using ten primers and 14 enzyme-probe
combinations respectively. The polymorphism observed for these markers revealed a high degree of genetic diversity in mangroves
at both inter-specific or inter-generic levels. A dendrogram, constructed after pooling both RAPD and RFLP data, using a similarity
index was analysed for genome relationships among these species. The dendrogram showed clustering of all the major mangroves,
except for Nypa fruticans (Arecaceae), into one group. All species under the tribe Rhizophorae formed a sub-cluster, to which Xylocarpus granatum was found to be the most closesly related species. The clustering pattern implied that Excoecaria agallocha and Acanthus ilicifolius should be considered as true minor mangroves. The present study also provided molecular data favouring the separation of
Avicennia spp. from the Verbenaceae to create a monotypic family the Avicenniaceae. The separation of Viscum orientale into the Viscaceae was also favoured.
Received: 5 March 1998 / Accepted: 1 April 1998 相似文献
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兰科(Orchidaceae)植物广布于除两极和极端沙漠地区外的各种陆地生态系统,包括5个亚科800多属28 000多种。东南亚地区兰科植物种数约占世界的1/3,是兰科植物生物多样性热点区域之一。通过查阅文献及书籍等资料,该文系统整理了东南亚兰科植物物种种类及其扩散演化历史,并对其生活习性和传粉系统进行了归类。结果表明:(1)东南亚兰科植物8 855种,分属5亚科17族26亚族240属;(2)主要生活型为附生的有127属6 000种以上,地生97属2 000种以上,腐生13属约100种,藤本4属40余种;(3)根据整理出的东南亚79个属的兰科植物传粉系统发现,有44个属含有自动自交的物种,具报酬物的传粉系统有花粉(仅见于拟兰亚科)、芳香类物质(仅见于香荚兰亚科)和花蜜(5个亚科均有)等报酬物类型。欺骗性传粉系统广泛存在于各个亚科,包括食源性欺骗、性拟态、繁殖地拟态和信息素拟态等类型。东南亚兰科植物在物种、生活习性及传粉系统都展现出极高的多样性,对这些生物学特点的总结将为兰科植物的保育提供一定的理论基础和本底资料。 相似文献
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Dietrich Schmidt-Vogt Stephen J. Leisz Ole Mertz Andreas Heinimann Thiha Thiha Peter Messerli Michael Epprecht Pham Van Cu Vu Kim Chi Martin Hardiono Truong M. Dao 《Human ecology: an interdisciplinary journal》2009,37(3):269-280
Swidden systems consisting of temporarily cultivated land and associated fallows often do not appear on land use maps or in
statistical records. This is partly due to the fact that swidden is a diverse and dynamic land use system that is difficult
to map and partly because of the practice of grouping land covers associated with swidden systems into land use or land cover
categories that are not self-evidently linked to swiddening. Additionally, in many parts of Southeast Asia swidden systems
have changed or are in the process of changing into other land use systems. This paper assesses the extent of swidden on the
basis of regional and national sources for nine countries, and determines the pattern of changes of swidden on the basis of
151 cases culled from 67 articles. Findings include (1) a majority of the cases document swidden being replaced by other forms
of agriculture or by other livelihood systems; (2) in cases where swiddening is still practiced, fallow lengths are usually,
but not always, shorter; and (3) shortened fallow length does not necessarily indicate a trend away from swidden since it
is observed that short fallow swidden is sometimes maintained along with other more intensive farming practices and not completely
abandoned. The paper concludes that there is a surprising lack of conclusive data on the extent of swidden in Southeast Asia.
In order to remedy this, methods are reviewed that may lead to more precise future assessments. 相似文献
16.
Rodel D.Lasco 《中国科学C辑(英文版)》2002,45(Z1)
Terrestrial ecosystems play an important role in the global carbon (C)cycle. Tropical forests in Southeast Asia are constantly changing as a result of harvesting and conversion to other land cover. As a result of these changes, research on C budgets of forest ecosystems has intensified in the region over thelast few years. This paper reviews and synthesizes the available information. Natural forests in SE Asia typically contain a high C density (up to 500 Mg/ha). Logging activities are responsible for at least 50% decline in forest C density.Complete deforestation (conversion from forest to grassland or annual crops) results in C density of less than 40 Mg/ha. Conversion to tree plantations and other woody perennial crops also reduces C density to less than 50% of the originalC forest stocks. While much information has been generated recently, there are still large gaps of information on C budgets of tropical forests and its conversion to other land uses in SE Asia. There is therefore a need to intensify research in this area. 相似文献
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Markku HAKKINEN Henry VARE 《植物分类学报》2008,46(1):89-92
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Restriction-site variation of PCR-amplified chloroplast DNA regions and its implication for the evolution and taxonomy of Actinidia 总被引:8,自引:0,他引:8
G. Cipriani R. Testolin R. Gardner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):389-396
Twenty six restriction sites from five PCR-amplified chloroplast DNA sequences (rbcL, psbA, rpoB, and two spacers flanking the trnL gene) were mapped and analysed in 20 Actinidia taxa, encompassing all four sections into which the genus is divided. At least three species out of the 20 examined have
been found to have originated through natural interspecific hybridisation on the basis of the discrepancy between morphological
and biochemical traits and the cpDNA profiles of pairs of species. A widely reticulate evolution has therefore been postulated
in Actinidia. Wagner and weighted parsimony analysis produced consensus trees that did not match the traditional taxonomy based on morphological
characters. The molecular data clearly showed that some taxa, such as A. rufa and A. kolomikta, occupy a wrong position and most, if not all, of the traditional groups represented by sections and series are weakly supported,
since they appear as polyphyletic. A. chinensis and A. deliciosa were confirmed to be very closely related. Since chloroplast DNA is paternally inherited in Actinidia, A. chinensis is a paternal progenitor, if not the only one, of A. deliciosa, the domesticated kiwifruit.
Received: 18 August 1997 / Accepted: 6 October 1997 相似文献
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Macaranga (Euphorbiaceae) includes about 280 species with a palaeotropic distribution. The genus not only comprises some of the most prominent pioneer tree species in Southeast Asian lowland dipterocarp forests, it also exhibits a substantial radiation of ant-plants (myrmecophytes). Obligate ant-plant mutualisms are formed by about 30 Macaranga species and 13 ant species of the genera Crematogaster or Camponotus. To improve our understanding of the co-evolution of the ants and their host plants, we aim at reconstructing comparative organellar phylogeographies of both partners across their distributional range. Preliminary evidence indicated that chloroplast DNA introgression among closely related Macaranga species might occur. We therefore constructed a comprehensive chloroplast genealogy based on DNA sequence data from the noncoding ccmp2, ccmp6, and atpB-rbcL regions for 144 individuals from 41 Macaranga species, covering all major evolutionary lineages within the three sections that contain myrmecophytes. A total of 88 chloroplast haplotypes were identified, and grouped into a statistical parsimony network that clearly distinguished sections and well-defined subsectional groups. Within these groups, the arrangement of haplotypes followed geographical rather than taxonomical criteria. Thus, up to six chloroplast haplotypes were found within single species, and up to seven species shared a single haplotype. The spatial distribution of the chloroplast types revealed several dispersals between the Malay Peninsula and Borneo, and a deep split between Sabah and the remainder of Borneo. Our large-scale chloroplast genealogy highlights the complex history of migration, hybridization, and speciation in the myrmecophytes of the genus Macaranga. It will serve as a guideline for adequate sampling and data interpretation in phylogeographic studies of individual Macaranga species and species groups. 相似文献