共查询到20条相似文献,搜索用时 15 毫秒
1.
Isolation of a factor, from the capsule of a granulosis virus, synergistic for a nuclear-polyhedrosis virus of the armyworm 总被引:1,自引:0,他引:1
When the capsules of a granulosis virus are fed together with the polyhedra of a nuclear-polyhedrosis virus to larvae of the armyworm, Pseudaletia unipuncta, the former enhances the infectivity of the latter virus, a synergistic interaction. The enhancement of infectivity depends upon the concentration of the polyhedra and the capsules. The factor responsible for the synergistic activity in the capsule can be dissolved in alkaline solution, separated from the virus particles by centrifugation, and further purified by Sephadex G-200 gel filtration with 4 m urea. The fraction obtained from Sephadex filtration and containing the synergistic factor can be separated into two components by disc-electrophoresis with 8 m urea. Both components possess synergistic activity. The ID50 of the synergistic factor corresponds to 0.0015 OD280. Its optimum pH is 8.5. Synergism is most evident when the factor is fed to larvae together with the polyhedra or is fed 24 hr prior to the ingestion of the polyhedra. The factor appears to be a simple or a conjugated protein of the capsule. 相似文献
2.
The dosage-mortality response of Hyphantria cunea larvae to a granulosis virus isolated from Diacrisia virginica was studied. Serial decimal dilutions of the D. virginica granulosis virus were fed to early second-instar H. cunea larvae. the LD50 for this virus (7.9 × 105 capsules/larva) was significantly greater than the LD50 calculated for the H. cunea granulosis virus (7.06 × 104 capsules/larva) against the same instar of H. cunea. Time mortality studies demonstrated that the LT50 values for the D. virginica granulosis were four to six times greater than for comparable dosages of H. cunea granulosis virus. Based on the mortality response of assayed H. cunea, it is possible to distinguish between the D. virginica granulosis virus and the H. cunea granulosis virus. 相似文献
3.
B M Pilley 《Journal of invertebrate pathology》1978,31(3):341-344
The interactions between the Diacrisia virginica granulosis virus (DGV) and the Hyphantria cunea baculovirus isolates were determined, utilizing defined differences between the time-mortality responses of these viruses fed to H. cunea larvae. The DGV, having a prolonged incubation period, when given an advantage in time or in the number of capsules, was able to prevent the expression of the more lethal H. cunea granulosis virus (HcGV) isolate. The time-mortality response of test larvae simultaneously fed equivalent dosages of HcGV and DGV was intermediate to that achieved with HcGV alone or DGV alone. Larvae infected with the DGV isolate were still susceptible to double infection by the nucleopolyhedrosis virus. The time-mortality response demonstrated that the development of nucleopolyhedrosis was only partly inhibited by preinfecting the test larvae with the DGV isolate. 相似文献
4.
The biochemical and biophysical characteristics of the closely related Diacrisia virginica and Hyphantria cunea granulosis virus isolates were examined. Sucrose gradient sedimentation patterns of alkali-solubilized DGV and HcGV capsules were identical. The top, middle, and bottom fractions from either viral isolate were infectious when injected into susceptible host larvae. Electrophoretic analysis of alkaline-solubilized granulin extracts demonstrated that both viruses contain alkaline proteolytic activity. The major granulin protein (~28,000 daltons) of both isolates comigrated in a SDS-PAGE. Electrophoretic separation of the virus proteins demonstrated some quantitative differences between the two granulosis viruses. The enveloped nucleocapsids and the nucleocapsids of the two viruses were morphologically indistinguishable. 相似文献
5.
The synergistic factor (SF) in the capsule of a granulosis virus (Hawaiian strain) of the armyworm, Pseudaletia unipuncta, contained polypeptides and phospholipids. Its molecular weight estimated by SDS-polyacrylamide gel electrophoresis was 126,000 ± 8,700. The capsule proteins were digested by a proteinase released from the capsule under alkaline conditions, and by trypsin added to the proteinase-free capsules. Neither enzyme affected the synergistic factor or its activity. The synergistic factor was slowly depolymerized by 2% sodium dodecyl sulfate and was more rapidly depolymerized when phospholipase C (phosphatidylcholine cholinephosphohydrolase) was also added. Phospholipase C alone did not decompose the synergistic factor, but it did destroy the capacity of the synergistic factor to enhance the nuclear polyhedrosis virus. In contrast, phospholipase A2 (phosphatidyl 2-acylhydrolase) had no effect on the synergistic factor. The different reactions of the two phospholipases on the synergistic factor suggested that the hydrophilic group of the phospholipid was exposed to the action of phospholipase C and was associated with the synergistic activity. This interpretation was supported by the detection of a phospholipid in the SF by thin-layer chromatography. 相似文献
6.
Different doses of a granulosis virus were administered to first- and fifth-instar larvae of the codling moth Laspeyresia pomonella. Virus was very pathogenic for both larval instars. The LD50 values for first- and fifth-instar larvae were 5 and 49 capsules/larva, respectively. However, fifth-instar larvae were much more variable in their response to virus than first-instar larvae. Using probit methods it was calculated that 1 capsule could cause death in about 25% of both larval instars but 1578 capsules were required to cause 70% mortality of fifth-instar larvae as compared to 12 capsules for first-instar larvae. This is the first report of a decided difference in variability of response to virus by two larval instars of the same species. A bimodal response by both larval instars was observed in time-mortality studies. Apparently, about 20% of the larvae were very resistant to virus infections. 相似文献
7.
Y. Tanada H. Inoue Roberta T. Hess Esther M. Omi 《Journal of invertebrate pathology》1980,35(3):249-255
A synergistic factor (SF), which is present in the capsule matrix protein of a granulosis virus of the armyworm, Pseudaletia unipuncta, enhances baculovirus infection in armyworm larvae. The site of action of the SF was investigated. The oral inoculation of SF did not enhance the infectious hemolymph virions which had been inoculated into the hemocoel. The SF also did not enhance the infection of purified enveloped virions when both virus and SF were inoculated into the hemocoel, but enhancement occurred when they were inoculated orally. Thus, the activity of the SF was confined to the midgut lumen. Observations with ferritin-conjugated antibody indicated that the site of action of SF was the cell membrane of the microvillus. There were more ferritin particles attached to midgut cell membranes of larvae inoculated orally with SF than to those of control larvae inoculated with buffer. 相似文献
8.
Christopher C. Payne G.Mark Tatchell Ceris F. Williams 《Journal of invertebrate pathology》1981,38(2):273-280
A comparison was made of the dosage-mortality responses of larvae of Pieris brassicae and P. rapae to infection by P. brassicae granulosis virus (GV). Bioassays with first, second, third, and fourth-instar larvae of both species revealed a marked difference in susceptibility between instars and between species. Median lethal dosages (LD50s) for P. rapae larvae ranged from five capsules for the first instar to 662 capsules for the fourth instar. With P. brassicae, this range extended from 66 capsules to 2.3 × 107 capsules. The time-mortality responses of the two species were similar when fed virus dosages equivalent to an LD90. Median lethal times (LT50s) ranged from 5 days for first-instar larvae to 7–8 days for fourth-instar larvae. A comparison between a long-established laboratory stock of P. brassicae and a stock recently acquired from the field showed no significant difference in their susceptibility to GV. The implications of the pronounced species differences in susceptibility to GV infection are discussed in relation to the potential field control of P. rapae and P. brassicae. 相似文献
9.
The synergistic (Hawaiian) strain of a granulosis virus (GV) from the armyworm, Pseudaletia unipuncta, was transmitted to Spodoptera exigua, Autographa californica, and Trichoplusia ni. The viruses isolated from these hosts were tested by radial double-immunodiffusion (RDD) and immunoelectrophoresis (IE) for their relationship to the original virus. Untreated and heat-treated virus inclusion bodies (capsules) were compared for their antigenic properties but no differences were detected. The antiserum elicited against the whole capsule was more sharply specific for the antigenic determinants than the one elicited against the dissolved capsule proteins. The viruses obtained from S. exigua and T. ni elicited precipitin lines that differed from those of the P. unipuncta GV in their electrophoretic mobility with the one-trough IE method; however, with the two-trough method, the lines fused indicating that the antigens were identical. The major precipitin line indicating identity of the viruses wasthat produced by the synergistic factor (SF) purified from the capsule proteins of the synergistic GV strain. The presence of SF in the GV produced in alternate hosts indicated that its production was virus directed. The SF was not detected in the GVs of Laspeyresia pomonella and Pieris rapae and in the nonsynergistic Oregonian GV of P. unipuncta. A field-collected GV from S. exigua exhibited a different precipitin pattern from that of the synergistic GV, but one of the precipitin lines shared partial relationship to the SF. 相似文献
10.
Methods for the solubilization of an insect granulosis virus capsule from Trichoplusia ni were studied using turbidity measurements. The solubilization in the solvents employed was most strongly influenced by pH. Various alcohols and hydrogen bond cleaving agents also enhanced solubilization. The most effective solvent tested at pH′s close to neutrality was 60% n-propanol saturated with guanidine. HCl. The effectiveness of this solvent was minimal at pH 5.2. It was shown that the time courses of the solubilization were generally composed of three phases. A plot of the third phase levels versus pH indicated a normal distribution of those capsules or capsular fragments in resisting solubilization. Validity of the calculation of rate constants for the dissociation reaction in the second phase was discussed. Logarithm of the rate constant showed a linear relationship with the reciprocal of absolute temperature. The activation energy for the dissociation calculated from this relationship using the Arrhenius equation indicated that hydrophobic and hydrogen binding forces play the major roles for the stability of the crystalline structure of the capsular protein. Inic binding forces were estimated to be of minor importance. 相似文献
11.
Norman E. Crook Jacqueline D. Brown Garth N. Foster 《Journal of invertebrate pathology》1982,40(2):221-227
A disease causing death in Lacanobia oleracea (Lepidoptera: Noctuidae) occurring in glasshouses in Scotland was shown to be caused by a granulosis virus (GV). Structural properties of the virus were examined by electron microscopy, immunodiffusion, polyacrylamide gel electrophoresis, and restriction endonuclease analysis and compared with an isolate of GV from L. oleracea obtained from France. The two isolates were structurally very similar but could be distinguished by analysis of EcoRI digests of their DNAs. Bioassays of the virus gave LD50 values from 104.3 capsules for second-instar larvae to 106.6 capsules for fifth-instar larvae. The French isolate was bioassayed in third-instar larvae and was not found to differ significantlyfrom the Scottish isolate. Two small glasshouse trials using the virus to control artificial infestations of L. oleracea indicated that high-volume sprays of virus at 108 to 109 capsules/ml achieved good control. An alternative strategy using much smaller amounts of virus to control the insect is discussed. 相似文献
12.
Plant pathogens that are dependent on arthropod vectors for transmission from host to host may enhance their own success by promoting vector survival and/or performance. The effect of pathogens on vectors may be direct or indirect, with indirect effects mediated by increases in host quality or reductions in the vulnerability of vectors to natural enemies. We investigated whether the bird cherry-oat aphid Rhopalosiphum padi, a vector of cereal yellow dwarf virus (CYDV) in wheat, experiences a reduction in rates of attack by the parasitoid wasp Aphidius colemani when actively harboring the plant pathogen. We manipulated the vector status of aphids (virus carrying or virus free) and evaluated the impact on the rate of attack by wasps. We found that vector status did not influence the survival or fecundity of aphids in the absence of parasitoids. However, virus-carrying aphids experienced higher rates of parasitism and greater overall population suppression by parasitoid wasps than virus-free aphids. Moreover, virus-carrying aphids were accepted as hosts by wasps more often than virus-free aphids, with a greater number of wasps stinging virus-carrying aphids following assessment by antennal palpations than virus-free aphids. Therefore, counter to the prevailing idea that persistent vector-borne pathogens enhance the performance of their vectors, we found that infectious aphids actively carrying a plant pathogen experience greater vulnerability to natural enemies. Our results suggest that parasitoids may contribute to the successful biological control of CYDV by disproportionately impacting virus-carrying vectors, and thus reducing the proportion of vectors in the population that are infectious. 相似文献
13.
A synergistic factor than enhances the infection of a nuclear polyhedrosis virus in the armyworm, Pseudaletia unipuncta, was isolated from the occlusion body (capsule) of a granulosis virus of the armyworm. Disc electrophoresis indicated that the purified factor was a single homogeneous compound. Chemical identification and amino acid analysis showed that it was a simple protein, with a molecular weight of 152,000–163,000. Proteolytic enzymes did not markedly reduce the activity of the factor. It could be stored at ?20°C or lyophilized. The synergistic factor displayed properties of an enzyme. It enhanced the hydrolysis of p-nitrophenyl esters of fatty acids with an optimum of pH 9.0. The relative hydrolytic activity increased with increase in number of carbon atoms in the fatty-acid chain from 2 to 8 and gradually decreased with the number of carbon atoms from 10 to 18. Copper sulfate markedly and mercuric chloride completely prevented enhancement of the hydrolysis of butyrate. When the synergistic factor was fed to larvae with mercuric chloride, it did not enhance the nuclear polyhedrosis virus. 相似文献
14.
Nguyen Ngoc Bao Chau Nguyen Quynh Phuong Anh Le Thuy To Nhu Nguyen Thi Phung Kieu Nguyen Bao Quoc 《Journal of Asia》2021,24(2):131-134
We investigated the influence of convenient diets on big-eyed bug Geocoris ochropterus. Development and reproduction of G. ochropterus fed on convenient diets of ant pupae Oecophylla smaragdina and silkworm pupae Bombyx mori were examined using aphids Aphis gossypii as the control diet. Results showed that Geocoris ochropterus nymphs completed development to adults on all diets. Total average development period was 35.1 days fed on ant pupae, 35.9 days fed on silkworm pupae, and 36.0 days fed on aphids. Head width, body length, forewing length, and fresh body weight of adults were not affected by diets, except for females reared on ant pupae that were significantly heavier than those fed on aphids. There was no significant difference in offspring sex ratio. Total number of eggs deposited per female fed on ant pupae was significantly larger than when fed on aphids, while eggs laid by females fed on silkworm pupae were significantly longer than eggs laid by females fed on aphids. Results suggest that ant pupae and silkworm pupae could be effectively used for mass rearing of G. ochropterus. 相似文献
15.
A purified granulosis virus isolated fromPieris brassicae (L.) was tested in the field against an introduced population ofPieris rapae (L.) larvae on cabbage (cv January King) in small experimental plots at Littlehampton, Sussex. Experiments were designed to compare the relative efficacy of single and multiple applications of virus (2.1×1012 and 3.7×1012 or 2.1×1014 and 3.7×1014 virus capsules/ha) in reducing numbers ofP. rapae larvae. An experiment was carried out in June 1978 and repeated in August to coincide approximately with the 2 natural generations ofP. rapae in southern England. Larval populations were monitored by regularin situ assessment of plants and by destructive sampling. Within 10 days of spraying virus there was a significant reduction in the mean larval population on all virus-treated plots compared with untreated controls. Sprays of 2.1×1014 and 3.7×1014 capsules/ha reduced larval numbers more quickly than 2.1×1012 and 3.7×1012/ha treatments. In the 1st experiment, three sprays of virus at either 2.1×1012 or 2.1×1014 capsules/ha gave no increase over the final level of control achieved by a single spray. However, in the 2nd experiment, a single spray of 3.7×1012 capsules/ha did not significantly reduce the numbers of larvae. It is likely that this failure could be accounted for by a combination of the larger “natural” population ofP. rapae recorded midway through the 2nd experiment and the rapid inactivation of virus deposits which left little infectious virus to infect these larvae. Virus inactivation was so rapid that only 7–33 % of the initial virus deposits remained 1 day after application. These results suggest that further understanding of virus formulation, persistence and dosage rates are needed before such a virus can be used in a rational manner. 相似文献
16.
The potato tuberworm Phthorimaea operculella (Zeller) is an important agricultural pest that causes significant economic losses to potato growers worldwide. The addition of an effective method of biological control for the potato tuberworm is greatly needed, and is currently unavailable in Brazil. The granulosis virus (Baculoviridae) is a promising biological control agent to protect post-harvest potatoes and in storage from the potato tuberworm. However, the control measure must be economically feasible. Liquid suspensions of a granulosis virus applied alone or in mixture with two commercial neem oil-based products (DalNeem? and NeemAzal?), and a dry powder formulation of viral granules were evaluated for control of potato tuberworm larvae by treating potato tubers under laboratory conditions. High larval mortality (86.7%) was achieved when DalNeem and virus were applied together at 4?mg of azadirachtin/L and 104 occlusion bodies (OBs)/mL, respectively. This combination resulted in ??50% efficacy in relation to their counterparts alone. Conversely, NeemAzal did not enhance virus effectiveness against larvae of the potato tuberworm. The talc-based virus formulation was used for dusting seed tubers at different concentrations and resulted in 100% larval mortality at 5?×?108 OBs/g. Formulated and unformulated virus provided 50% mortality at 166 OBs/g and at 5.0?×?105 OBs/mL, respectively. As a result, talc-based virus formulation had a better control efficiency on potato tuberworm than the aqueous virus suspension. The granulosis virus combined with DalNeem at low rates or formulated with talc powder is a viable option to control the potato tuberworm under storage conditions. 相似文献
17.
Characterization of an extremely basic protein derived from granulosis virus nucleocapsids 总被引:11,自引:8,他引:3
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Nucleocapsids were isolated from purified enveloped nucleocapsids of Plodia interpunctella granulosis virus by treatment with Nonidet P-40. When analyzed on sodium dodecyl sulfate-polyacrylamide gels, the nucleocapsids consisted of eight polypeptides. One of these, a major component with a molecular weight of 12,500 (VP12), was selectively extracted from the nucleocapsids with 0.25 M sulfuric acid. Its electrophoretic mobility on acetic acid-urea gels was intermediate to that of cellular histones and protamine. Amino acid analysis showed that 39% of the amino acid residues of VP12 were basic: 27% were arginine and 12% were histidine. The remaining residues consisted primarily of serine, valine, and isoleucine. Proteins of similar arginine content also were extracted from the granulosis virus of Pieris rapae and from the nuclear polyhedrosis viruses of Spodoptera frugiperda and Autographa californica. The basic polypeptide appeared to be virus specific because it was found in nucleocapsids and virus-infected cells but not in uninfected cells. VP12 was not present in polypeptide profiles of granulosis virus capsids, indicating that it was an internal or core protein of the nucleocapsids. Electron microscopic observations suggested that the basic protein was associated with the viral DNA in the form of a DNA-protein complex. 相似文献
18.
Clayton C. Beegle 《Journal of invertebrate pathology》1978,32(3):325-331
A granulosis virus was found infecting Plathypena scabra larvae in Iowa. The capsules averaged 377 ± 25 × 222 ± 19 nm. On the basis of light microscopical observations, the virus appeared to infect the epidermis, fat body, and tracheal matrix cells. The LC50 and LC95 were 6.7 × 107 and 1.5 × 109 capsules/acre, respectively. The LT50 values varied from 3 to 9 days for 1 × 1012 and 1 × 108 capsules/acre, respectively. 相似文献
19.
Capsules of a granulosis virus of the codling moth were never found in codling moth eggs, although they were observed in the larval, pupal, and adult stages. However, eggs obtained from a British Columbia codling moth colony were found to have virus on the egg surface.Transstadial transmission of the virus from the larval to the pupal stage occasionally occurred after intact capsules had been inoculated per os into the larva. The virus was also occasionally transmitted from the pupal to the adult stage, but only after virions had been injected into the hemocoel of the pupa.Fertile eggs obtained from virus-injected insects were not shown to contain active virus. Eggs were tested by maceration in antiserum, by the fluorescent-antibody technique, and by bioassay of their contents.Only one larva of the F1 and F2 generations obtained from virus-injected insects succumbed to granulosis even when the larvae were exposed to various types of stressors.Circumstantial evidence supports the hypothesis of transovum transmission, but such transmission cannot be attributed to injections of virus into larvae and pupae in this study. 相似文献
20.
Biological control and soybean cultivars bred for increased resistance to the soybean aphid (Aphis glycines) are two approaches used to manage this serious pest of soybeans in North America. However, as with many other pest systems, the compatibility of these two pest management approaches has not been studied in detail. The aphidiine wasp Aphidius colemani is one of several candidate species for biological control of the soybean aphid in soybean in North America. Resistance to the soybean aphid in the USDA soybean cultivar Dowling is largely controlled by a single dominant gene Rag1, which is the focus of plant breeding programs directed against the soybean aphid. In this study, we measured developmental and behavioral differences in the parasitic wasp A. colemani when it attacked soybean aphids feeding on either the aphid-resistant Dowling or aphid-susceptible Glenwood cultivars of soybean. We used a combination of choice and no-choice experiments to examine the effects of host plant cultivar on the number of parasitized aphids formed and the sex ratio and body weights of adult offspring produced. Significantly more aphids were parasitized when they fed on Glenwood compared to Dowling and these offspring were larger when they developed in aphids that fed on Glenwood soybeans. To distinguish between effects on foraging decisions and offspring survivorship, we conducted an additional experiment that followed the oviposition decisions and fate of each parasitized aphid. Foraging female A. colemani spent less time handling individual aphids and encountered and attacked aphids at a higher rate when they fed on aphids feeding on Glenwood soybeans than aphids feeding on Dowling soybeans. Furthermore, wasp survivorship in aphids was greater on Glenwood than Dowling. Taken together, aphid-resistance in soybeans has negative effects on foraging behavior and offspring fitness of A. colemani raising concerns about the compatibility of these two pest management approaches. 相似文献