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1.
Gene Biziulevičienė Vytautas Kašėta Giedrė Ramanauskaitė Aida Vaitkuvienė 《Central European Journal of Biology》2010,5(5):585-589
The objective of this work was to examine the migration of transplanted bone marrow hematopoietic lin− cell population using the BALB/c mouse contact hypersensitivity model in vivo and to determine the time point at which they reach the site of injury (paw edema) as well as other undamaged organs, such
as liver and spleen. Female BALB/c mice with induced contact hypersensitivity reaction were intravenously injected with 1×106 cells/mouse lin− cells, labeled with PKH67. The presence of lin− stained cells in mouse tissue sections was evaluated by fluorescent microscopy. After one hour, the labeled cells were found
in mice paw edema and liver, and after 4 hours in spleen tissue. Migrated hematopoietic lin− cells remained in liver tissue for 48 h, and in spleen and paw edema at least for 72 h. Migrated stained cells in untreated
paw were not found. The results prove that bone marrow unmatured hematopoietic cells are first found in paw edema, where they
participate in the inhibition of tissue inflammation; these cells subsequently migrate to the liver and are found in the spleen
shortly afterwards. 相似文献
2.
Kanokporn Noy Rithidech Paiboon Reungpatthanaphong Louise Honikel Adam Rusek Sanford R. Simon 《Radiation and environmental biophysics》2010,49(3):405-419
The objective of this study was to determine the kinetics of nuclear factor-kappa B (NF-κB) activation and cytokine expression
in bone marrow (BM) cells of exposed mice as a function of the dose rate of protons. The cytokines included in this study
are pro-inflammatory [i.e., tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and IL-6] and anti-inflammatory
cytokines (i.e., IL-4 and IL-10). We gave male BALB/cJ mice a whole-body exposure to 0 (sham-controls) or 1.0 Gy of 100 MeV
protons, delivered at 5 or 10 mGy min−1, the dose and dose rates found during solar particle events in space. As a reference radiation, groups of mice were exposed
to 0 (sham-controls) or 1 Gy of 137Cs γ rays (10 mGy min−1). After irradiation, BM cells were collected at 1.5, 3, 24 h, and 1 month for analyses (five mice per treatment group per
harvest time). The results indicated that the in vivo time course of effects induced by a single dose of 1 Gy of 100 MeV protons
or 137Cs γ rays, delivered at 10 mGy min−1, was similar. Although statistically significant levels of NF-κB activation and pro-inflammatory cytokines in BM cells of
exposed mice when compared to those in the corresponding sham controls (Student’s t-test, p < 0.05 or <0.01) were induced by either dose rate, these levels varied over time for each protein. Further, only a dose rate
of 5 mGy min−1 induced significant levels of anti-inflammatory cytokines. The results indicate dose-rate effects of protons. 相似文献
3.
Stanislav Filip Jaroslav Mokrý Jiřina Vávrová Zuzana Šinkorová Stanislav Mičuda Pavel Šponer Alžběta Filipová Hana Hrebíková Govindan Dayanithi 《Journal of cellular and molecular medicine》2014,18(5):832-843
Bone marrow–derived cells represent a heterogeneous cell population containing haematopoietic stem and progenitor cells. These cells have been identified as potential candidates for use in cell therapy for the regeneration of damaged tissues caused by trauma, degenerative diseases, ischaemia and inflammation or cancer treatment. In our study, we examined a model using whole-body irradiation and the transplantation of bone marrow (BM) or haematopoietic stem cells (HSCs) to study the repair of haematopoiesis, extramedullary haematopoiesis and the migration of green fluorescent protein (GFP+) transplanted cells into non-haematopoietic tissues. We investigated the repair of damage to the BM, peripheral blood, spleen and thymus and assessed the ability of this treatment to induce the entry of BM cells or GFP+lin−Sca-1+ cells into non-haematopoietic tissues. The transplantation of BM cells or GFP+lin−Sca-1+ cells from GFP transgenic mice successfully repopulated haematopoiesis and the haematopoietic niche in haematopoietic tissues, specifically the BM, spleen and thymus. The transplanted GFP+ cells also entered the gastrointestinal tract (GIT) following whole-body irradiation. Our results demonstrate that whole-body irradiation does not significantly alter the integrity of tissues such as those in the small intestine and liver. Whole-body irradiation also induced myeloablation and chimerism in tissues, and induced the entry of transplanted cells into the small intestine and liver. This result demonstrates that grafted BM cells or GFP+lin−Sca-1+ cells are not transient in the GIT. Thus, these transplanted cells could be used for the long-term treatment of various pathologies or as a one-time treatment option if myeloablation-induced chimerism alone is not sufficient to induce the entry of transplanted cells into non-haematopoietic tissues. 相似文献
4.
To date, there is scant information on in vivo induction of chromosomal damage by heavy ions found in space (i.e. 56Fe ions). For radiation-induced response to be useful for risk assessment, it must be established in in vivo systems especially
in cells that are known to be at risk for health problems associated with radiation exposure (such as hematopoietic cells,
the known target tissue for radiation-induced leukemia). In this study, the whole genome multicolor fluorescence in situ hybridization
(mFISH) technique was used to examine the in vivo induction of chromosomal damage in hematopoietic tissues, i.e. bone marrow
cells. These cells were collected from CBA/CaJ mice at day 7 following whole-body exposure to different doses of 1 GeV/amu
56Fe ions (0, 0.1, 0.5 and 1.0 Gy) or 137Cs γ rays as the reference radiation (0, 0.5, 1.0 and 3.0 Gy, at the dose rate of 0.72 Gy/min using a GammaCell40). These
radiation doses were the average total-body doses. For each radiation type, there were four mice per dose. Several types of
aberrations in bone marrow cells collected from mice exposed to either type of radiation were found. These were exchanges
and breaks (both chromatid- and chromosome-types). Chromosomal exchanges included translocations (Robertsonian or centric
fusion, reciprocal and incomplete types), and dicentrics. No evidence of a non-random involvement of specific chromosomes
in any type of aberrations observed in mice exposed to 56Fe ions or 137Cs γ rays was found. At the radiation dose range used in our in vivo study, the majority of exchanges were simple. Complex
exchanges were detected in bone marrow cells collected from mice exposed to 1 Gy of 56Fe ions or 3 Gy of 137Cs γ rays only, but their frequencies were low. Overall, our in vivo data indicate that the frequency of complex chromosome
exchanges was not significantly different between bone marrow cells collected from mice exposed to 56Fe ions or 137Cs γ rays. Each type of radiation induced significant dose-dependent increases (ANOVA, P < 0.01) in the frequencies of chromosomal damage, including the numbers of abnormal cells. Based upon the linear-terms of
dose-response curves, 56Fe ions were 1.6 (all types of exchanges), 4.3 (abnormal cells) and 4.2 (breaks, both chromatid- and chromosome-types) times
more effective than 137Cs γ rays in inducing chromosomal damage. 相似文献
5.
France Bruck Ludovic Belle Chantal Lechanteur Laurence de Leval Muriel Hannon Sophie Dubois Emilie Castermans Stephanie Humblet-Baron Souad Rahmouni Yves Beguin Alexandra Briquet Frédéric Baron 《Cytotherapy》2013,15(3):267-279
Background aimsGraft-versus-host disease (GVHD) is a life-threatening complication of allogeneic hematopoietic cell transplantation caused by donor T cells reacting against host tissues. Previous studies have suggested that mesenchymal stromal cells (MSCs) could exert potent immunosuppressive effects.MethodsThe ability of human bone marrow derived MSCs to prevent xenogeneic GVHD in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice and in NOD/SCID/interleukin-2Rγ(null) (NSG) mice transplanted with human peripheral blood mononuclear cells (PBMCs) was assessed.ResultsInjection of 200 × 106 human PBMCs intraperitoneally (IP) into sub-lethally (3.0 Gy) irradiated NOD/SCID mice also given anti-asialo GM1 antibodies IP 1 day prior and 8 days after transplantation induced lethal xenogeneic GVHD in all tested mice. Co-injection of 2 × 106 MSCs IP on day 0 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs. Similarly, injection of 30 × 106 human PBMCs IP into sub-lethally (2.5 Gy) irradiated NSG mice induced a lethal xenogeneic GVHD in all tested mice. Injection of 3 × 106 MSCs IP on days 0, 7, 14 and 21 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs.ConclusionsInjection of MSCs did not prevent xenogeneic GVHD in these two humanized mice models. 相似文献
6.
A. Dumas M. A. Le Drévo M. F. Moreau C. Guillet M. F. Baslé D. Chappard 《Cytotechnology》2008,58(3):163-171
Selection of cells having the most osteogenic potential is a strategy used in bone tissue engineering. Preclinical studies
using murine bone marrow cells must consider the large amount of hematopoietic cells in the adherent fraction. The aim of
this study was to enrich a murine bone marrow cell population with osteoprogenitors by using a simple and reliable method.
Bone marrow from C57Bl/6 mice was extracted and cells which adhered onto plastic were expanded in primary culture for 14 days.
Immunolabeling of the CD11b surface antigen was performed and the CD11b− cell fraction was isolated by FACS. Sorted and unsorted populations were analyzed for gene expression of osteoblast differentiation,
alkaline phosphatase (AlkP) activity and matrix mineralization capacities. Selection of CD11b− cells increased the number of AlkP+ cells from the plastic adherent fraction from 6.3% ± 0.8 to 56% ± 3.3 with a sevenfold increase in AlkP activity. mRNA analysis
revealed a significant increase in the CD11b− fraction for Osterix (41-fold), RANKL (17-fold), M-CSF (8-fold) and Runx-2 (8-fold). An osteogenic population was obtained
with improved capacities to produce a mineralized extracellular matrix in vitro, independently of the presence of glucocorticoids
in the culture medium. 相似文献
7.
Li WB Gerstmann U Giussani A Oeh U Paretzke HG 《Radiation and environmental biophysics》2008,47(1):101-110
The mysterious death of Mr. Alexander Litvinenko who was most possibly poisoned by Polonium-210 (210Po) in November 2006 in London attracted the attention of the public to the kinetics, dosimetry and the risk of this high
radiotoxic isotope in the human body. In the present paper, the urinary excretion of seven persons who were possibly exposed
to traces of 210Po was monitored. The values measured in the GSF Radioanalytical Laboratory are in the range of natural background concentration.
To assess the effective dose received by those persons, the time-dependence of the organ equivalent dose and the effective
dose after acute ingestion and inhalation of 210Po were calculated using the biokinetic model for polonium (Po) recommended by the International Commission on Radiological
Protection (ICRP) and the one recently published by Leggett and Eckerman (L&E). The daily urinary excretion to effective dose
conversion factors for ingestion and inhalation were evaluated based on the ICRP and L&E models for members of the public.
The ingestion (inhalation) effective dose per unit intake integrated over one day is 1.7 × 10−8 (1.4 × 10−7) Sv Bq−1, 2.0 × 10−7 (9.6 × 10−7) Sv Bq−1 over 10 days, 5.2 × 10−7 (2.0 × 10−6) Sv Bq−1 over 30 days and 1.0 × 10−6 (3.0 × 10−6) Sv Bq−1 over 100 days. The daily urinary excretions after acute ingestion (inhalation) of 1 Bq of 210Po are 1.1 × 10−3 (1.0 × 10−4) on day 1, 2.0 × 10−3 (1.9 × 10−4) on day 10, 1.3 × 10−3 (1.7 × 10−4) on day 30 and 3.6 × 10−4 (8.3 × 10−5) Bq d−1 on day 100, respectively. The resulting committed effective doses range from 2.1 × 10−3 to 1.7 × 10−2 mSv by an assumption of ingestion and from 5.5 × 10−2 to 4.5 × 10−1 mSv by inhalation. For the case of Mr. Litvinenko, the mean organ absorbed dose as a function of time was calculated using
both the above stated models. The red bone marrow, the kidneys and the liver were considered as the critical organs. Assuming
a value of lethal absorbed dose of 5 Gy to the bone marrow, 6 Gy to the kidneys and 8 Gy to the liver, the amount of 210Po which Mr. Litvinenko might have ingested is therefore estimated to range from 27 to 1,408 MBq, i.e 0.2–8.5 μg, depending
on the modality of intake and on different assumptions about blood absorption. 相似文献
8.
The programmed, stepwise acquisition of immunocompetence that marks the development of the fetal immune response proceeds
during a period when both T cell receptor and immunoglobulin (Ig) repertoires exhibit reduced junctional diversity due to
physiologic terminal deoxynucleotidyl transferase (TdT) insufficiency. To test the effect of N addition on humoral responses,
we transplanted bone marrow from TdT-deficient (TdT−/−) and wild-type (TdT+/+) BALB/c mice into recombination activation gene 1-deficient BALB/c hosts. Mice transplanted with TdT−/− cells exhibited diminished humoral responses to the T-independent antigens α-1-dextran and (2,4,6-trinitrophenyl) hapten
conjugated to AminoEthylCarboxymethyl-FICOLL, to the T-dependent antigens NP19CGG and hen egg lysozyme, and to Enterobacter cloacae, a commensal bacteria that can become an opportunistic pathogen in immature and immunocompromised hosts. An exception to
this pattern of reduction was the T-independent anti-phosphorylcholine response to Streptococcus pneumoniae, which is normally dominated by the N-deficient T15 idiotype. Most of the humoral immune responses in the recipients of TdT−/− bone marrow were impaired, yet population of the blood with B and T cells occurred more rapidly. To further test the effect
of N-deficiency on B cell and T cell population growth, transplanted TdT-sufficient and -deficient BALB/c IgMa and congenic TdT-sufficient CB17 IgMb bone marrow were placed in competition. TdT−/− cells demonstrated an advantage in populating the bone marrow, the spleen, and the peritoneal cavity. TdT deficiency, which
characterizes fetal lymphocytes, thus appears to facilitate filling both central and peripheral lymphoid compartments, but
at the cost of altered responses to a broad set of antigens. 相似文献
9.
Yamaguchi M Hamamoto R Uchiyama S Ishiyama K 《Molecular and cellular biochemistry》2007,303(1-2):83-88
The effect of various flavonoids, which are present in food and plants, on bone calcium content and osteoclastogenesis were
investigated to compare action of flavonoid on bone formation and bone resorption in vitro. Rat femoral-diaphyseal (cortical
bone) and -metaphyseal (trabecular bone) tissues were cultured for 48 h in Dulbecco’s modified Eagle’s medium (high glucose)
supplemented with antibiotics and bovine serum albumin. Amoung quercetin, myricetin, kaempferol, isorhamnetin, curcumin, hesperidin,
or astaxanthin in the range of 10−7–10−5 M, culture with quercetin (10−6 or 10−5 M) caused a significant increase in diaphyseal calcium content. Such an effect was not seen in other compounds. Mouse bone
marrow cells were cultured for 7 days in the presence of parathyroid hormone (PTH; 10−7 M), a bone-resorbing factor, in vitro. Culture with PTH caused a significant increase in osteoclast-like cell formation. This
increase was significantly inhibited in the presence of quercetin, myricetin, kaempferol, isorhamnetin, or curcumin in the
range of 10−8–10−6 M. Such an effect was not seen in the case of hesperidin or astaxanthin. In addition, culture with PTH (10−7 M) caused a significant decrease in diaphyseal calcium content. This decrease was completely prevented in the presence of
quercetin, myricetin, kaempferal, or isorhamnetin of 10−6 M. This study demonstrates that various flavonoids have a potent inhibitory effect on osteoclastogenesis and bone resorption
rather than bone formation in vitro. Among various flavonoids, quercetin had a stimulatory effect on bone formation and an
inhibitory effect on bone resorption in vitro. 相似文献
10.
《Journal of thermal biology》2002,27(1):29-37
Studies were carried out to gain an insight into the mechanisms underlying WBH induced radioprotection. The plasma levels of IL-1α, IL-6, TNF-α and GM-CSF, were elevated in WBH treated mice between 2 and 6 h after treatment. The total nucleated cell count of hemopoietic tissues such as spleen, thymus, bone marrow and peripheral blood showed drastic reduction without recovery until death in mice treated with TBI. However, the nucleated cell count in the above tissues showed significant recovery after initial drop in WBH and WBH+TBI treated groups and reached to a normal level by day 7 and day 28, respectively. The total WBC and RBC count in peripheral blood recovered to a control level by day 28 after treatment. Significant number of endogenous spleen colonies were detected, 14 days after TBI in WBH pre-treated mice whereas no such spleen colonies could be detected in TBI treated group. The transplantation of bone marrow derived from control, WBH, TBI and WBH+TBI treated groups of mice to lethally irradiated mice (8 Gy) showed formation of spleen colonies only in mice which received bone marrow from control, WBH and WBH+TBI treated groups. Transplantation of the bone marrow from these groups of mice resulted in prolonged survival of lethally irradiated mice as compared to mice receiving bone marrow from TBI treated mice. These results seem to suggest that WBH induced radioprotection of mice could be due to immunomodulation manifested through induction of cytokines responsible for protection and proliferative response, leading to accelerated recovery from hemopoietic damage-a major cause of radiation induced death. 相似文献
11.
Tolstykh EI Shagina NB Degteva MO Anspaugh LR Napier BA 《Radiation and environmental biophysics》2011,50(3):417-430
The Mayak Production Association released large amounts of 90Sr into the Techa River (Southern Urals, Russia) with peak amounts in 1950–1951. Techa Riverside residents ingested an average
of about 3,000 kBq of 90Sr. The 90Sr-body burden of approximately 15,000 individuals has been measured in the Urals Research Center for Radiation Medicine in
1974–1997 with use of a special whole-body counter (WBC). Strontium-90 had mainly deposited in the cortical part of the skeleton
by 25 years following intake, and 90Sr elimination occurs as a result of cortical bone resorption. The effect of 90Sr-radiation exposure on the rate of cortical bone resorption was studied. Data on 2,022 WBC measurements were selected for
207 adult persons, who were measured three or more times before they were 50–55 years old. The individual-resorption rates
were calculated with the rate of strontium recirculation evaluated as 0.0018 year−1. Individual absorbed doses in red bone marrow (RBM) and bone surface (BS) were also calculated. Statistically significant
negative relationships of cortical bone resorption rate were discovered related to 90Sr-body burden and dose absorbed in the RBM or the BS. The response appears to have a threshold of about 1.5-Gy RBM dose.
The radiation-induced decrease in bone resorption rate may not be significant in terms of health. However, a decrease in bone
remodeling rate can be among several causes of an increased level of degenerative dystrophic bone pathology in exposed persons. 相似文献
12.
Dubravka Štajner Boris M. Popović Ksenija Taški 《Central European Journal of Biology》2009,4(3):381-386
There are some reports that low doses of γ-irradiation could induce antioxidant activities in plant material, including soybean.
Irradiation, required for the inactivation of some pathogens and induction of mutations, may have adverse effects on sensorial,
nutritional and antioxidant qualities. The effects of different γ-irradiation doses (100–200 Gy) on antioxidant properties
of soybean seeds was investigated. In this study, we report the results obtained by analysis of antioxidant enzyme activities,
reduced glutathione, malonyldialdehyde (MDA) and hydroxyl (HO−) radical quantities, soluble protein content, and total antioxidant activity in irradiated soybean seeds. Antioxidant enzyme
activities were affected due to high irradiation intensity. Significant changes of total antioxidant activity and MDA and
HO.quantities were observed only under the highest irradiation dose, with a 15.7% reduction in total antioxidant activity,
MDA quantity increase of 21.6%, and HO− radical quantity increase of 79.3% compared to the non-irradiated control. The total soluble protein content increased slightly. 相似文献
13.
Guenterberg KD Lesinski GB Mundy-Bosse BL Karpa VI Jaime-Ramirez AC Wei L Carson WE 《Cancer immunology, immunotherapy : CII》2011,60(9):1281-1288
Interferon-alpha (IFN-α) is an immunomodulatory cytokine that is used clinically for the treatment of melanoma in the adjuvant
setting. The cellular actions of IFN-α are regulated by the suppressors of cytokine signaling (SOCS) family of proteins. We
hypothesized that the anti-tumor activity of exogenous IFN-α would be enhanced in SOCS1-deficient mice. SOCS1-deficient (SOCS1−/−) or control (SOCS1+/+) mice on an IFN-γ−/− C57BL/6 background bearing intraperitoneal (i.p.) JB/MS murine melanoma cells were treated for 30 days with i.p. injections
of IFN-A/D or PBS (vehicle). Log-rank Kaplan-Meier survival curves were used to evaluate survival. Tumor-bearing control SOCS1+/+ mice receiving IFN-A/D had significantly enhanced survival versus PBS–treated mice (P = 0.0048). The anti-tumor effects of IFN-A/D therapy were significantly enhanced in tumor-bearing SOCS1−/− mice; 75% of these mice survived tumor challenge, whereas PBS-treated SOCS1−/− mice all died at 13-16 days (P = 0.00038). Antibody (Ab) depletion of CD8+ T cells abrogated the anti-tumor effects of IFN-A/D in SOCS1−/− mice as compared with mice receiving a control antibody (P = 0.0021). CD4+ T-cell depletion from SOCS1−/− mice also inhibited the effects of IFN-A/D (P = 0.0003). IFN-A/D did not alter expression of CD80 or CD86 on splenocytes of SOCS1+/+ or SOCS1−/− mice, or the proportion of T regulatory cells or myeloid-derived suppressor cells in SOCS1+/+ or SOCS1−/− mice. An analysis of T-cell function did reveal increased proliferation of SOCS1-deficient splenocytes at baseline and in
response to mitogenic stimuli. These data suggest that modulation of SOCS1 function in T-cell subsets could enhance the anti-tumor
effects of IFN-α in the setting of melanoma. 相似文献
14.
15.
K. I. Altman H. Mühlensiepen R. Wolters O. Muzik L. E. Feinendegen 《Radiation and environmental biophysics》1993,32(1):59-64
We showed previously that the Rb+ transport rate in bone marrow cells (BMC) of vitamin-E-deficient mice is significantly lower than that in BMC of euvitaminotic mice. It is now evident that 4 h after whole-body, low-dose (0.01–1.0 Gy) gamma-irradiation of avitaminotic mice, there is an increase in the rate of Rb+ transport. This increase is quite pronounced, exceeding at all dose levels the rate of Rb+ transport in euvitaminotic mice exposed to the same radiation dose.On leave from the University of Rochester, School of Medicine and Dentistry, Department of Biophysics, Rochester, New York, USA 相似文献
16.
Abstract
We measured bacterial growth on phototransformed dissolved organic matter (DOM) leached from eight different primary producers.
Leachates (10 mg C liter−1) were exposed to artificial UVA + UVB radiation, or kept in darkness, for 20 h. DOM solutions were subsequently inoculated
with lake water bacteria. Photoproduction of dissolved inorganic carbon (DIC), ranging from 3 to 16 μg C liter−1 h−1, and changes in the absorptive characteristics of the DOM were observed for all leachates upon UV irradiation. The effects
of irradiation exposure on DOM bioavailability varied greatly, depending on leachate and type of bacterial growth criterion.
Bacterial carbon utilization (biomass production plus respiration) over the entire incubation period (120 h) was enhanced
by UV radiation of leachate from the terrestrial leaves, relative to carbon utilization in non-irradiated leachates. Conversely,
carbon utilization was reduced by radiation of the leachates from aquatic macrophytes. In a separate experiment, the stable
C and N isotope composition of bacteria grown on irradiated and non-irradiated DOM was estimated. Bacterial growth on UV-irradiated
DOM was enriched in 13C relative to the bacteria in the non-irradiated treatments; this result may be explained by selective assimilation of photochemically
produced, isotopically enriched labile compounds.
Received: 17 February 2000; Accepted: 1 May 2000; Online Publication: 28 August 2000 相似文献
17.
PurposeThe radioprotective effects of Dragon's blood (DB) and its extracts (DBE) were investigated using the chromosomal aberrant test, micronucleus and oxidative stress assay for anti-clastogenic and anti-oxidative activity.Materials and methodsAdult BALB/C mice were exposed to the whole body irradiation with 4 Gy 60Co γ-rays. DB and DBE were administered orally once a day from 5 days prior to irradiation treatment to 1 day after irradiation. The mice were sacrificed on 24 h after irradiation. The cells of bone marrow were measured by counting different types of chromosomal aberrations and the frequency of micronuclei. Oxidative stress response was carried out by analysis of serum from blood.ResultsDB and DBE significantly decreased the number of bone marrow cells with chromosome aberrations after irradiation with respect to irradiated alone group. The administration of DB and DBE also significantly reduced the frequencies of micronucleated polychromatic erythrocytes (MPCE) and micronucleated normochromatic erythrocytes (MNCE). In addition, DB and DBE markedly increased the activity of antioxidant enzymes and the level of antioxidant molecular. Malondialdehyde (MDA) and nitric oxide (NO) levels in serum were significantly reduced by DB and DBE treatment.ConclusionsOur data suggested that DB and DBE have potential radioprotective properties in mouse bone marrow after 60Co γ-ray exposure, which support their candidature as a potential radioprotective agent. 相似文献
18.
K. Barth C. Pfleger A. Linge J. A. Sim A. Surprenant N. Steinbronn R. H. Strasser M. Kasper 《Histochemistry and cell biology》2010,134(1):31-38
It has recently been shown in epithelial cells that the ATP-gated ion channel P2X7R is in part, associated with caveolae and
colocalized with caveolin-1. In the present study of the mouse heart, we show for the first time, using immunohistochemistry
and cryoimmunoelectron microscopy, that P2X7R is expressed in atrial cardiomyocytes and in cardiac microvascular endothelial
cells, but not in the ventricle cardiomyocytes. Furthermore, biochemical data indicate the presence of two forms of P2X7R,
the classical glycosylated 80 kDa isoform and a protein with the molecular weight of 56 kDa, in both cardiomyocytes and endothelial
cells of the mouse heart. The functionality of both proteins in heart cells is still unclear. In cardiac tissue homogenates
derived from caveolin-1 deficient mice (cav-1
−/−), an increase of the P2Xrx7 mRNA and P2X7R protein (80 kDa) was found, particularly in atrial samples. In addition, P2rx7
−/− mice showed enhanced protein levels of caveolin-1 in their atrial tissues. Although the details of cellular mechanisms that
underlie the relationship between caveolin-1 and P2X7R in atrial cardiomyocytes and the electrophysiological consequences
of the increased P2X7R expression in atrial cells of cav-1
−/− mice remain to be elucidated, the cardiomyopathy detectable in cav-1
−/− mice is possibly related to a disturbed crosstalk between P2X7R and caveolin-1 in different heart cell populations. 相似文献
19.
K H W?odarski 《Folia biologica》1990,38(1-4):49-56
The activity of alkaline and acid phosphatases in the bone marrow from the femoral cavity was investigated in the following groups of mice: (1) normal (non-irradiated); (2) irradiated with 600 R; (3) irradiated and repopulated with syngeneic bone marrow; (4) irradiated and repopulated with syngeneic marrow stroma; (5) non-irradiated, infused with allogeneic bone marrow (host versus graft reaction, HvG); (6) irradiated and repopulated with allogeneic bone marrow (graft versus host reaction, GvH). In addition, the activity of alkaline and acid phosphatases was examined in bone marrow stromal cultures. In irradiated animals the activity of both enzymes was lower than in non-irradiated ones, repopulation with syngeneic bone marrow restoring it to normal. Repopulation with allogeneic marrow (GvH) resulted in a very deep reduction of alkaline, but not acid, phosphatase. It is postulated that the decrease in bone marrow alkaline phosphatase activity can be a sensitive test for the early GvH reaction, preceding such parameters as splenomegaly. Marrow stroma cultured in vitro also showed very low alkaline phosphatase activity. 相似文献
20.
T cell clones (CD4+CD8–TCRαβ+γδ–) derived from bone marrow transplant recipients were stimulated with phytohaemagglutinin (PHA) +interleukin-2 (IL-2) in the
presence of irradiated (50 Gy) peripheral blood mononuclear cells (PBMC) derived from acute leukaemia patients(leukaemic PBMC
containing more than 95% blast cells). Leukaemic PBMC could function as accessory cells during mitogenic T cell activation
resulting in both T cell proliferation and a broad T cell cytokine response [IL-3, IL-4, IL-10, granulocyte/macrophage-colony-stimulating
factor (GM-CSF) tumour necrosis factor α (TNFα) and interferon γ (IFNγ) secretion]. Blockade of IL-1 effects by adding IL-1
receptor antagonist together with PHA+IL-2+leukaemia blasts increased T cell proliferation, whereas IL-6-neutralizing antibodies
did not alter T cell proliferation. A qualitatively similar T cell cytokine response and a similar cytokine profile (highest
levels detected for GM-CSF and IFNγ) were detected when normal polyclonal T cell lines were stimulated with PHA in the presence
of non-irradiated leukaemic PBMC. When leukaemic PBMC derived from 18 acute myelogenous leukaemia patients were cultured with
PHA and cells from a polyclonal T cell line, increased concentrations of the T cell cytokines IFNγ and IL-4 were detected
for all patients. We conclude that T cell activation resulting in proliferation and a broad cytokine response can take place
in the presence of excess acute myelogenous leukaemia blasts.
Received: 30 November 1995 / Accepted: 9 January 1996 相似文献