北京和湖南烟粉虱生物型及其抗药性监测

烟粉虱是蔬菜等园艺作物上为害严重、最难以防治的害虫之一,生产上防治该虫主要还是依靠化学药剂。本研究鉴定了北京、湖南地区设施蔬菜上烟粉虱的生物型,并监测了其对6种不同杀虫剂的抗药性。结果表明,北京、湖南地区蔬菜烟粉虱生物型分别是Q型和B型。两地烟粉虱对阿维菌素尚处于敏感状态;对烯啶虫胺及其他烟碱类杀虫剂噻虫嗪和吡虫啉的抗药性达中抗到高抗水平,湖南地区抗性水平比北京地区更高,抗性倍数最高达到71.58倍;传统杀虫剂毒死蜱和联苯菊酯对湖南、北京两地烟粉虱的毒杀活性差。     

Polymorphic adaptive insecticidal resistance in Rhynocoris marginatus (Fabr.) (Het., Reduviidae) a non-target biocontrol agent

Rhynocoris marginatus (Fabr.) is a polymorphic reduviid predator inhabitating tropical rainforests, semi-arid zones, scrub jungles and agro-ecosystems and predates upon insect pests. It exists in three different morphs; (1) with black connexivum (niger); (2) with red connexivum (sanguineous); and (3) with black and red banded connexivum (nigrosanguineous). Insecticides used to manage insect pests affect non-target biocontrol agents such as R. marginatus . Hence laboratory experiments were conducted to evaluate the adaptive polymorphic resistance of the morphs of R. marginatus to the toxic effects of three insecticides, namely, dimethoate, methylparathion and quinalphos, with each in five concentrations. The 50% lethal concentration (LC₅₀) values clearly indicated that methylparathion was the most toxic insecticide followed by quinalphos and endosulfan to all three morphs. Among the three morphs, the niger was the most resistant and the sanguineous was the most sensitive.     

Response of old and new cowpea varieties to insecticide spray regimes in the Sudan savanna of Nigeria

Insect pests are major constraint to cowpea production in northern Nigeria causing yield losses up to 70%. Several cowpea varieties have been developed and delivered to farmers by IITA over the past four decades. These varieties have varying degrees of resistance to insect pest attacks. A field study was established in northern Nigeria to determine the response to insecticide spraying of old cowpea varieties developed in the late 1970s and new varieties developed in the 2000s in order to determine whether new varieties have lesser requirement for spraying with insecticides than the old ones. The result revealed that the new cowpea varieties developed in the 2000s require more spraying than the old varieties developed in the 1970s. Infestations by the insect pests, maruca (Maruca virata Fabricius) and flower thrips (Megalurothrips sjostedti Trybom) were significantly less at zero and 1 spray for old varieties than for the new varieties. Old varieties also produced significant higher grain yield at zero and 1 spray compared with new varieties. Newer varieties require more spray to maximise yield gain.     

Gas-exchange characteristics,leaf water potential and chlorophyll <Emphasis Type="Italic">a</Emphasis> fluorescence in oil palm (<Emphasis Type="Italic">Elaeis guineensis</Emphasis> Jacq.) seedlings under water stress and recovery

The gas-exchange characteristics, leaf water potential and chlorophyll (Chl) a fluorescence of oil palm (Elaeis guineensis Jacq.) seedlings subjected to water stress and recovery were investigated in a greenhouse experiment. At 24 days after imposition of stress, leaf water potential in water-stressed seedlings was doubled compared to that of control and there was a drastic decline in gas-exchange parameters viz. photosynthesis, transpiration, and stomatal conductance. Water stress did not irreversibly affect gas-exchange parameters and quantum efficiency of photosystem II, as seedlings exhibited total recovery of photosynthetic apparatus by 12^th day of rehydration. These findings indicate that oil palm exhibits physiological plasticity to water stress during the seedling stage.     

亚致死剂量杀虫剂对异色瓢虫捕食作用的影响

为探讨害虫化学防治与生物防治的协调技术,提高综合防治水平,测定了吡虫啉,鱼藤酮,氰戊菊酯和阿维菌素A种杀虫剂亚致死剂量对异色瓢虫成虫捕食桃蚜功能反应的影响。亚致死剂量杀虫剂对异色瓢虫功能反应模型的基本结构没有改变,但影响到了模型的各项参数,药剂处理后异色瓢虫最大日捕食量降低,处理猎物的时间延长,捕食速率和寻找效应一般也被减弱,影响结果还与异色瓢虫受药的方式有关,4种药剂中以氰戊菊酯的影响最大,鱼藤酮的胃毒作用也较明显,研究结果表明亚致死剂量的杀虫剂对天敌异色瓢虫的捕食作用存在着不良影响,这为害虫治理中如何协调利用化学防治和生物防治措施,指导科学用药提供了一定的理论基础。     

Activity of an essential oil derived from Chenopodium ambrosioides on greenhouse insect pests

This study involved both greenhouse and laboratory experiments evaluating the effect of an essential oil product (QRD 400) derived from Chenopodium ambrosioides variety nr. Ambrosioides L. (Chenopodiaceae) on greenhouse insect pests that feed on different plant parts: citrus mealybug, Planococcus citri (Risso); longtailed mealybug, Pseudococcus longispinus (Targioni Tozzetti); western flower thrips, Frankliniella occidentalis (Pergande), and fungus gnats (Bradysia spp.). Treatments were applied to coleus, Solenostemon scutellarioides plants; transvaal daisy, Gerbera jamesonii flowers; or growing medium, depending on the insect pest. The essential oil was most effective, based on adult emergence, on both the second and third instars of the fungus gnat Bradysia sp. nr. coprophila when applied as a drench to growing medium. In addition, there was a significant rate response for QRD 400 on fungus gnats. The QRD 400 treatment had the highest percentage of mortality on longtailed mealybug (55%) compared with the other treatments. However, the essential oil was less effective against citrus mealybug (3% mortality) and western flower thrips adults (18-34% mortality) compared with standard insecticides, such as acetamiprid (TriStar) and spinosad (Conserve), which are typically used by greenhouse producers. This lack of efficacy may be associated with volatility and short residual properties of the essential oil or with the essential oil taking longer to kill insect pests. Other insecticides and miticides evaluated, including sesame oil, garlic, paraffinic oil, and Bacillus thuringiensis subsp. israelensis, provided minimal control of the designated insect pests. In addition, adult rove beetle Atheta coriaria Kraatz adults were not effective in controlling the larval instars of fungus gnats when applied at a rate of five adults per container.     

无公害水稻生产的病虫草调控技术及其效应的研究

利用无公害农药和农业措施防治水稻主要病虫草试验结果表明,井·腊芽、井冈霉素和多抗霉素在水稻分蘖末期和孕穗末期各喷施1次,对纹枯病防效达75.16％～94．27％,优于化学杀菌剂三唑酮;春雷霉素和灭瘟素在叶瘟病发生初期及破肚期、齐穗期各喷施1次,对叶瘟防效为50．54％～72．67％,穗瘟为76．66％～8742％,与化学杀菌剂三环唑相当3种无公害农药在二化螟和稻纵卷叶螟1～2龄幼虫期使用,苏云金杆菌防效优于化学杀虫剂杀虫双,皂素·烟碱和苦参碱与杀虫双相当;皂素·烟碱和苦参碱在稻飞虱1～2龄若虫期使用,药后3～30d防效均在70％以上,低于化学杀虫剂吡虫啉,在水稻移栽前耕地2次,再配合施用适量的未腐熟有机质或人工除草,能控制杂草发生和提高产量,采用无公害农药和农业措施防治水稻病虫草,产量较常规化防区略有增加,稻谷中农药残留量较化防区低,符合绿色食品卫生指标要求。     

Population dynamics of Spodoptera litura (Lepidoptera: Noctuidae) on Bt cotton in the Yangtze River Valley of China

Genetically modified cotton that produces a crystalline protein from Bacillus thuringiensis subsp. kurstaki (Berliner) (Bt) has been widely deployed to manage lepidopteran insect pests in cotton growing areas worldwide. However, susceptibility of different insect species to Bt protein varies, which may affect lepidopteran pest populations in the field. Studies on effects of two transgenic cotton lines (BG1560 and GK19) carrying a Cry1A gene on common cutworm Spodoptera litura F. (Lepidoptera: Noctuidae), were conducted during 2002-2005 in the cotton planting region of the Yangtze River valley of China. Results showed that common cutworm larvae had low susceptibility to Bt cotton. There was no significant difference in larval population densities in conventional and Bt cotton fields. However, the larval populations of the insect on conventional plants treated with chemical insecticides for control of target pest of Bt cotton were significantly lower than that in Bt cotton fields. These results indicated that the common cutworm was the potential to become a major and alarming pest in Bt cotton fields, and therefore efforts to develop an effective alternative management strategy are needed.     

槟榔苗期主要病虫害为害症状及防治方法

槟榔幼苗健壮程度直接影响槟榔植株前期的生长速度、中后期的果品品质及最终的果实产量.本文综述了槟榔幼苗期发生的主要生理性病害(白化、日灼、肥害)、侵染性病害(炭疽病、大茎点霉叶斑病、枯萎病)以及虫害(椰圆蚧、基斑毒蛾、斑腿蝗虫、红蜘蛛、黑刺粉虱)的发生症状、防治方法等.根据槟榔育苗期病虫害的发生规律,探索各种病害、虫害的防治方法,以期为槟榔苗期病虫害防治提供参考.     

Conserving the efficacy of insecticides against Plutella xylostella (L.) (Lep., Plutellidae)

Abstract:  The diamondback moth (DBM), Plutella xylostella (L.) (Lep., Plutellidae), is one of the most destructive insect pests of crucifers worldwide. It was the first crop insect reported to be resistant to DDT and now in many crucifer-producing regions it has shown significant resistance to almost every insecticide applied in field including biopesticides such as crystal toxins from Bacillus thuringiensis and spinosyns from Saccharopolyspora spinosa . In certain parts of the world, economical production of crucifers has become almost impossible because of its resistance to insecticides and resulting control failure. A coordinated resistance management program needs to be implemented with the involvement of pesticide industry, local pesticide regulatory authorities, scientists and farmers. The judicious use of chemicals in conjunction with other control measures (e.g. biological control agents, resistant varieties, proper fertilization rates) is the best way to manage DBM and other pests of cruciferous crops. Introduction of glucosinolate-sulphatase inhibitors as plant-incorporated-products or sprayable material may also lead to a novel pest management strategy.     

昆虫抗药性产生机制

杀虫剂是害虫防治的有效途径之一,但随着杀虫剂长期和广泛的使用,昆虫种群对各种杀虫剂的敏感性降低,产生了抗药性,如何克服昆虫的抗药性是害虫综合治理的重要问题。近年来,借助基因组测序和遗传操作技术的发展,对昆虫抗药性的研究已经深入到细胞水平和分子水平,取得诸多重要的突破,为害虫抗性的控制奠定了理论基础。本文从常见杀虫剂的历史沿革及作用机理切入,从靶标抗性、代谢抗性和穿透抗性3个方面阐述了杀虫剂抗性产生的机制:杀虫剂作用位点的突变降低了靶标与杀虫剂的亲和力,细胞色素P450酶系和谷胱甘肽转移酶系的激活增加了杀虫剂的降解,表皮结构成分的变化和ABC转运蛋白的增加有效阻挡了杀虫剂的渗入。利用基因操作手段或抑制剂,对上述3种抗性机制的关键步骤进行调控可能成为未来杀虫剂抗性控制的新策略。     

Influence of Frequently Used Chemical Insecticides on Mycoflora Carried by Common Housefly, <Emphasis Type="Italic">Musca domestica</Emphasis> L.

The housefly, Musca domestica L. (Diptera: Muscidae), is a major medical and veterinary insect pest. It serves as a vector of many pathogenic microorganisms causing spoilage of food and diseases in human and animals. Use of chemical insecticides is adapted as a principal tool to manage housefly. Insecticides have many unforeseen ecological consequences including effects on non-target organisms. In the present study, we have assessed the effects of 10 different synthetic insecticides on the growth of mycoflora associated with the external body of the housefly by using poison food technique. Our results reveled that all synthetic insecticides enhanced the growth. Surprisingly, in most of the cases, mycelial growth of fungi was significantly increased at high concentration as compared with lower concentration. This study provides useful information about the dangerous effects of synthetic insecticides on environment by increasing the spread of various non-target pathogenic, mycotoxigenic, and food spoiling fungi, carried by houseflies.     

Impact of light-emitting diode irradiation on photosynthesis,phytochemical composition and mineral element content of lettuce cv. Grizzly

Light-emitting diodes (LEDs) are a promising technology with a potential to improve the irradiance efficiency, light quality, and the light spectrum for increasing plant yield and quality. In this experiment, we investigated the impacts of various LED light qualities, including 100% red, 100% blue, 70% red + 30% blue, and 100% white, on the growth and photosynthesis, phytochemical contents, and mineral element concentrations in lettuce (Lactuca sativa L. cv. ‘Grizzly’) in comparison to normal greenhouse conditions. Photon flux of 300 µmol m^?2 s^?1 was provided for 14 h by 120 LEDs set on a 60 cm × 60 cm sheet of aluminum platform in the growth chambers, where plants were grown for 60 d. Fresh mass per plant was significantly higher when grown under 100% blue and 70% red + 30% blue LEDs compared to the other environments including greenhouse conditions. Phytochemical concentrations and a nutritive value of lettuce were also significantly affected by the light treatments. Chlorophyll and carotenoid concentrations increased in the plants grown under 70% red + 30% blue LEDs compared to those grown in the greenhouse. Vitamin C content was 2.25-fold higher in the plants grown under 100% blue LEDs compared to those grown in the greenhouse. Higher photosynthesis and maximal quantum yield of PSII photochemistry were also observed in the plants treated with LED lights. The application of LED light led to the elevated concentrations of macro-and micronutrients in lettuce possibly because of the direct effect of LED light and lower stress conditions in the growth chambers compared to the greenhouse. Although the mechanism of the changes in lettuce grown under LED is not well understood, the results of this study demonstrated that LED light could be used to enhance the growth and nutritional value of lettuce in indoor plant production facilities.     

山东水稻病虫害系统最优控制研究

1994～1996年研究了稻田13种可控因素对水稻病虫害、天敌和产量的综合效应以及分期混合施药技术。根据既控制病虫增产又保护生态的原则,综合评价了可控因素,提出了水稻病虫害系统最优控制为中国91或辐4-2,N150kg/hm     

Effects of pirimicarb,buprofezin and pymetrozine on survival,development and reproduction of Coccinella undecimpunctata (Coleoptera: Coccinellidae)

The effects of pirimicarb (a neurotoxin), buprofezin (an insect growth regulator) and pymetrozine (an antifeedant) on Coccinella undecimpunctata were assessed by studying the survival and development of all immature stages and the survival and reproductive performance of adults. Insecticides were sprayed at doses recommended by the manufacturers for the control of aphids and/or whiteflies. None of the three insecticides had a significant effect on the survival of C. undecimpunctata eggs. When sprayed on larvae, buprofezin significantly reduced survival to adulthood to <33%, compared to >45% for the control and other insecticide treatments. Rates of adult survival, fecundity, and fertility, and the percentage of egg hatch, were not significantly different between control and insecticide treatments. Thus, larval stages were more susceptible to insecticides than were adults. In general, pirimicarb and pymetrozine had no adverse effects on immature or adult stages of C. undecimpunctata, and hence are suitable for IPM of sucking pests.     

Current status and prospects on microbial control in Japan

Historically in Japan, studies on the diseases of the silkworm, Bombyx mori, as a factor affecting the well-being of the silk industry, have dominated insect pathology. However, work by Hidaka in 1933 demonstrated the possibility of controlling the pine moth, Dendrolimus spectabilis, with the fungus Beauveria bassiana and since then, various attempts have been made to develop a method to control insect pests using insect pathogens. The cypovirus product, Matsukemin, was the first microbial control product to be registered in 1974, and inactive and live Bacillus thuringiensis products were also registered and put on the market as pesticides in 1981 and 1982, respectively. Currently, there are 25 microbial insecticides on the market that constitute slightly less than 2% of all insecticides used in Japan. Adoption of biopesticides is likely to increase in the near future due to scientific advances and several new government policies that encourage the use of alternative pest control products.     

Beyond the spore--past and future developments of Bacillus thuringiensis as a biopesticide

Formulated and sporulated cultures of Bacillus thuringiensis (Bt) are widely used as foliar sprays as part of integrated pest management strategies against insect pests of agricultural crops. Although in several cases the presence of the spore has been shown to improve the activity of the product, other Bt-based insecticides have been developed in which the spore is absent. The most notable of these are transgenic plants expressing just the insect toxin gene from the bacterium. This paper will discuss these developments, and the advantages and disadvantages of having the spore present.     

Evaluation of soil‐applied insecticides with Bt maize for managing corn rootworm larval injury

The western corn rootworm, Diabrotica virgifera virgifera LeConte, is considered one of the most significant insect pests of maize in North America. Larvae of other secondary subterranean pests such as grape colaspis, Colaspis brunnea (F.), and Japanese beetle, Popillia japonica Newman, can also injure maize seedlings and cause yield loss. In the past decade, maize hybrids containing Bt proteins have been used to manage the western corn rootworm; additionally, seeds are commonly treated with a neonicotinoid and fungicide combination to control secondary pests. Recently, soil‐applied insecticides have been used in conjunction with rootworm Bt hybrids (and seed‐applied insecticides) in areas with perceived risk for increased rootworm larval or secondary pest damage. We conducted a series of trials from 2009 to 2011 that examined multiple rootworm Bt hybrids and their near‐isolines, along with two soil‐applied insecticides, to determine whether the Bt plus insecticide combination resulted in an increased level of efficacy or yield. We also sampled for Japanese beetle and grape colaspis larvae to determine their potential for reducing yield. Densities of secondary pests in our trials were low and likely had no effect on maize yield. The addition of a soil‐applied insecticide to rootworm Bt hybrids improved efficacy only once across 17 location‐years, when overall corn rootworm injury was highest; an improvement in yield was never observed. Our results suggest that the use of a soil‐applied insecticide with a rootworm Bt hybrid should only be considered in scenarios with potentially significant rootworm larval populations. However, potential negative consequences related to trait durability when soil insecticides are used with rootworm Bt maize should be considered.     

The biotechnology of Bacillus thuringiensis

One of the challenges in the application of biotechnology to pest control is the identification of agents found in nature which can be used effectively. Biotechnology offers the potential of developing pesticides based on such agents which will provide environmentally sound and economically feasible insect control alternatives. Such an agent, the insect pathogen Bacillus thuringiensis, is the subject of intense investigations in several laboratories. Insecticides which use the entomocidal properties of B. thuringiensis are currently produced and sold worldwide; new products are currently in the development stage. Herein, the biology and genetics of B. thuringiensis and the problems associated with current products are critically reviewed with respect to biotechnology. Moreover, the economic and regulatory implications of technologically advanced products are evaluated.     

菜田节肢动物群落特征及其多样性恢复的研究

探讨了化学杀虫剂对菜田节肢动物群落结构和多样性的影响以及停止化学杀虫剂使用后多样性的恢复,结果表明化学杀虫剂对菜田节肢动物群落的物种组成,多样性指数及物种分布的均匀性等均有影响,菜田节肢动物群落结构变化同害虫类,捕食性天敌和蜘蛛类的特征变化关系密切,经常使用化学杀虫剂的菜田与停止使用后的菜田相比,物种数量显减少,多样性指数降低,停止化学杀虫剂使用后,优势害虫的种群趋势指数明显降低,大敌对主要害虫的控制作用增强,节肢动物多样性指数增大,因此,限制化学杀虫剂的使用,恢复由于化学杀虫剂的使用而破坏了菜田环境,增强天敌对主要害虫的控制能力,这在菜田害虫生态控制中起着重要的作用。