甘肃双盾木属一新种

甘肃双盾木属一新种王一峰,廉永善,王国宏（西北师范大学生物系,兰州７３００７０）关键词双盾木属,文县双盾木,甘肃ＡＮＥＷＳＰＥＣＩＥＳＯＦＴＨＥＧＥＮＵＳＤＩＰＥＬＴＡＭＡＸＩＭ．ＦＲＯＭＧＡＮＳＵ￥ＷａｎｇＹｉｆｅｎｇ;ＬｉａｎＹｏｎｇｓｈａｎａｎ...     

虎耳草属一新亚种

虎耳草属一新亚种王国宏,廉永善,王一峰（西北师范大学生物系,兰州７３００７０）关键词虎耳草属,甘肃虎耳草ＡＮＥＷＳＵＢＳＰＥＣＩＥＳＯＦＳＡＸＩＦＲＡＧＡＦＲＯＭＣＨＩＮＡ￥ＷａｎｇＧｕｏｈｏｎｇ;ＬｉａｎＹｏｎｇｓｈａｎａｎｄＷａｎｇＹｉｆｅｎｇ（...     

披针叶胡颓子果实营养成分的测定

披针叶胡颓子果实营养成分的测定毛学文（天水师范高等专科学校,天水７４１０００）ＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＮＵＴＲＩＥＮＴＣＯＮＳＴＩＴＵＥＮＴＳＩＮＳＥＥＤＳＯＦＥＬＡＥＡＧＮＵＳＬＡＮＣＥＯＬＡＴＡＭａｏＸｕｅ－ｗｅｎ（ＴｉａｎＳｈｕｉＴｅａｃ...     

中国异肢螨属一新纪录和美绥螨属一新纪录（蜱螨亚纲：革螨股）

中国异肢螨属一新纪录和美绥螨属一新纪录（蜱螨亚纲：革螨股）马立名（吉林省地方病第一防治研究所白城１３７０００）ＡＮＥＷＲＥＣＯＲＤＯＦＰＯＥＣＩＬＯＣＨＩＲＵＳＡＮＤＡＮＥＷＲＥＣＯＲＤＯＦＡＭＥＲＯＳＥＩＵＳＦＲＯＭＣＨＩＮＡ（ＡＣＡＲＩ：ＧＡＭＡ...     

被子植物核型胚乳细胞化机理研究的现状

被子植物核型胚乳细胞化机理研究的现状李师翁（庆阳师专生物系,甘肃西峰７４５０００）ＡＤＶＡＮＣＥＳＩＮＴＨＥＳＴＵＤＩＥＳＯＮＴＨＥＭＥＣＨＡＮＩＳＭＯＦＣＥＬＬＵＬＡＲＩＺＡＴＩＯＮＯＦＮＵＣＬＥＡＲＥＮＤＯＳＰＥＲＭＩＮＡＮＧＩＯＳＰＥＲＭＳＬｉ...     

长体茧蜂属中国五新记录种

长体茧蜂属中国五新记录种ＮＥＷＲＥＣＯＲＤＳＯＦＭＡＣＲＯＣＥＮＴＲＵＳＣＵＲＴＩＳ（ＨＹＭＥＮＯＰＴＥＲＡ：ＢＲＡＣＯＮＩＤＡＥ：ＭＡＣＲＯＣＥＮＴＲＩＮＡＥ）ＦＲＯＭＣＨＩＮＡ￥长体茧蜂属ＭａｃｒｏｃｅｎｔｒｕｓＣｕｒｔｉｓ,１８３３隶于茧蜂科长...     

福建悬钩子属一新种

福建悬钩子属一新种张永田,陈丽云（福建省亚热带植物研究所,厦门３６１００６）关键词悬钩子属,九仙莓,新种ＡＮＥＷＳＰＥＣＩＥＳＯＦＲＵＢＵＳ（ＲＯＳＡＣＥＡＥ）ＦＲＯＭＦＵＪＩＡＮ￥ＺｈａｎｇＹｏｎｇｔｉａｎａｎｄＣｈｅｎＬｉｙｕｎ（ＦｕｊｉａｎＩｎ...     

中国跗线螨属二新纪录：蜱螨亚纲：跗线螨科

中国跗线螨属二新纪录（蜱螨亚纲：跗线螨科）林坚贞,张艳璇（福建省农科院植保所福州３５００１３）范青海（福建农业大学植保系福州３５０００２）ＴＷＯＮＥＷＲＥＣＯＲＤＳＯＦＴＡＲＳＯＮＥＭＵＳＦＲＯＭＣＨＩＮＡ（ＡＣＡＲＩ：ＴＡＲＳＯＮＥＭＩＤＡＥ）￥Ｌ...     

广西天南星属一新种

广西天南星属一新种刘寿养（广西中医学院,南宁５３０００１）关键词天南星属;桂西南星ＡＮＥＷＳＰＥＣＩＥＳＯＦＧＥＮＵＳＡＲＩＳＡＥＭＡＭＡＲＴ．ＦＲＯＭＧＵＡＮＧＸＩ￥ＬｉｕＳｈｏｕｙａｎｇ（ＧｕａｎｇｘｉＣｏｌｌｅｇｅｏｆＴｒａｄｉｔｉｏｎａｌＣｈ...     

不同光质对毛地黄愈伤组织诱导和增殖的效应

不同光质对毛地黄愈伤组织诱导和增殖的效应毛学文陈荃（天水师范高等专科学校生物系,天水７４１０００）ＥＦＦＥＣＴＳＯＦＬＩＧＨＴＱＵＡＬＩＴＹＯＮＣＡＬＬＵＳＩＮＤＵＣＴＩＯＮＡＮＤＧＲＯＷＴＨＯＦＤＩＧＩＴＡＬＳＰＵＲＰＵＲＥＡＭａｏＸｕｅ－ｗｅｎＣ...     

Discrimination Amongst Leishmania by Polymerase Chain Reaction and Hybridization with Small Subunit Ribosomal DNA Derived Oligonucleotides

ABSTRACT. A method for discriminating among Leishmania is described, based upon small subunit ribosomal DNA sequence differences. The method was to amplify the entire 2.2 kb small subunit rDNA by polymerase chain reaction using conserved primers specific for the 5' and 3' termini of the small subunit ribosomal RNA, and then hybridize the product dotted onto nylon membranes with labeled oligonucleotides. The design of the hybridization probes was based upon complete small subunit rDNA sequences from L. amazonensis, L. major and L. guyanensis and partial sequences of L. mexicana, L. braziliensis, L. tropica and L. chagasi. A high degree of sequence similarity (> 99%) among species was found. However, sufficient sequence divergence occurred to permit the design of internal oligonucleotide probes specific for species complexes. This procedure successfully discriminated amongst a wide range of Leishmania isolates. The method detected as few as 10 cultured organisms and detected parasites in tissue samples from experimentally infected animals. Non-radioactive labeling showed the same specificity and sensitivity as radioactive probes.     

东北地区乳菇属的初步研究

本文报道了东北地区乳菇属(Lactarius)的29种,其中包括新种2个,长白乳菇(L.chanbainensis Y.Wang et Xie sp.nov.)温泉乳菇(L.wenquanensis Y.Wang et Xie sp.nov.);国内新记录种5个:茶绿乳菇[L.necator(Pers.ex Fr.)Farst.],条纹乳菇[L.oculatus(Peck)Burl.],变红乳菇[L.acris(Bolt.ex Fr.)Gray],复生乳菇(L.repraesentaneus Britz.)和点柄乳菇(L.maculatus Burl.)。新种有汉文和拉丁文描述以及形态、显微构造图;国内新记录种有汉文描述。     

Novel multiplex polymerase chain reaction primer set for identification of Lactococcus species

Aims: The gram‐positive bacterial genus Lactococcus has been taxonomically classified into seven species (Lactococcus lactis, Lactococcus garvieae, Lactococcus piscium, Lactococcus plantarum, Lactococcus raffinolactis, Lactococcus chungangensis and Lactococcus fujiensis). This study aimed to develop a novel multiplex polymerase chain reaction (PCR) primer set for the identification of the seven lactococcal species, as well as to differentiate the two industrially important dairy subspecies, L. lactis subsp. lactis and L. lactis subsp. cremoris. Methods and Results: A multiplex PCR primer set was designed based on the nucleotide sequences of the 16S rRNA gene of the seven lactococcal species. The specificity of the established one‐step multiplex PCR scheme was verified using more than 200 bacterial strains, in which a complete sequence match was confirmed by partial sequencing of their 16S rRNA gene. Conclusions: The one‐step multiplex PCR enables the identification and speciation of bacterial strains belonging to the genus Lactococcus and the differentiation of strains of L. lactis subsp. lactis and L. lactis subsp. cremoris. Significance and Impact of the Study: This work provides an efficient method for identification of lactococcal strains of industrial importance.     

松树上的七种散斑壳*

本文报道松树上散斑壳属(Lophodermium Chev)的7个种,其中3个新种:安徽散斑壳(L.anhuiense Y.R.Lin sp.nov.)、白皮松散斑壳(L.pini—bungeanae Y.R.Lin sp.nov.)及奇异散斑壳(L.mirabile Y.R.Lin sp.nov.);2个我国新记录种:针叶树散斑壳(L.conigenum(Brunaud)Hilitz.)和南方散斑壳(L.australe Dearn.);2个国内已记载的种:松针散斑壳(L.pinastri(schrad.)Chev.)和乔松散斑壳(L.pini-excelsae Ahmad)。文中列出了分种检索表,对新种作了拉丁文和汉文描述,对新记录种的主要特点以及已知种的寄主新记录和地理新分布分别作了记载。     

松树上的七种散斑壳

本文报道松树上散斑壳属(Lophodermium Chev)的7个种,其中3个新种:安徽散斑壳(L.anhuiense Y.R.Lin sp.nov.)、白皮松散斑壳(L.pini—bungeanae Y.R.Lin sp.nov.)及奇异散斑壳(L.mirabile Y.R.Lin sp.nov.);2个我国新记录种:针叶树散斑壳(L.conigenum(Brunaud)Hilitz.)和南方散斑壳(L.australe Dearn.);2个国内已记载的种:松针散斑壳(L.pinastri(schrad.)Chev.)和乔松散斑壳(L.pini-excelsae Ahmad)。文中列出了分种检索表,对新种作了拉丁文和汉文描述,对新记录种的主要特点以及已知种的寄主新记录和地理新分布分别作了记载。     

Taxonomical Revision of Some Species of Lunathyrium Koidzumi in N. E. Asia

This paper deals with some species of Lunathyrium Koidz. in N. E. Asia; including the eastern mountainous district of N. E. China; Far East Region of U. S. S. R.; Korea and Japan.     

中国疱脐衣属的新种与新资料

本文报道了中国疱脐衣属四个新分类单位,其中包括三个新种:华东疱脐衣(Lasallia sinorientalis Wei),大理疱脐衣(L.daliensis Wei),苍山疱脐衣(L.caeonshanensis Wei)及东亚疱脐衣一新变种:樊净山变种(L.asiae-orientalis var.fanjingensis Wei)。此外,还从东亚疱脐衣的模式标本中首次发现了该种的子囊盘,并作了补充描述。作者认为,根据有关中国地衣区系的现有知识,迄今尚未发现分布于中国的疱脐衣(Lasallia pustulata(L.)Merat)。而华东疱脐衣可能是疱脐衣在亚洲的地理替代种。     

两种过路黄的核型研究

对国产报春花科珍珠菜属植物过路黄和疏节过路黄的核型进行了首次报道。过路黄染色体数目为２ｎ＝２４,核型公式为２ｎ＝２４＝２ｍ＋４ｓｍ＋６ｓｔ＋１２ｔ,核型类型“３Ａ”;疏节过路黄染色体数目为２ｎ＝２２,核型公式为２ｎ＝２２＝４ｍ＋６ｓｍ＋１０ｔ,核型类型“３Ａ”。     

两种过路黄的核型研究

对国产报春花科（Ｐｒｉｍｕｌａｃｅａｅ）珍珠菜属（Ｌｙｓｉｍａｃｈｉａ）植物过路黄（Ｌ.ｃｈｒｉｓｔｉｎａｅＨａｎｃｅ）和疏节过路黄（Ｌ.ｒｅｍｏｔａＰｅｔｉｔｍ）的核型进行了首次报道。过路黄染色体数目为２ｎ＝２４,核型公式为２ｎ＝２４＝２ｍ＋４ｓｍ＋６ｓｔ＋１２ｔ,核型类型“３Ａ”;疏节过路黄染色体数目为２ｎ＝２２,核型公式为２ｎ＝２２＝４ｍ＋６ｓｍ＋２ｓｔ＋１０ｔ,核型类型“３Ａ”。