Notch signaling in the mammalian respiratory system,specifically the trachea and lungs,in development,homeostasis, regeneration,and disease

The respiratory system has ideal tissue structure and cell types for efficient gas exchange to intake oxygen and release carbon dioxide. This complex system develops through orchestrated intercellular signaling among various cell types, such as club, ciliated, basal, neuroendocrine, AT1, AT2, endothelial, and smooth muscle cells. Notch signaling is a highly conserved cell–cell signaling pathway ideally suited for very short-range cellular communication because Notch signals are transmitted by direct contact with an adjacent cell. Enthusiastic efforts by Notch researchers over the last two decades have led to the identification of critical roles of this signaling pathway during development, homeostasis, and regeneration of the respiratory system. The dysregulation of Notch signaling results in a wide range of respiratory diseases such as pulmonary artery hypertension (PAH), chronic obstructive pulmonary disease (COPD), interstitial pulmonary fibrosis (IPF), and lung cancer. Thus, a deep understanding of the biological functions of Notch signaling will help identify novel treatment targets in various respiratory diseases.     

Cholinesterases and arylesterase in the umbilical cord, the placenta, and the amniotic membrane, in the female at term]

Cholinesterasic activity of umbilical cord (tissue), completely bloodless, is exclusively due to pseudocholinesterase. Cholinesterase is more active in placenta than in cord; it is an acetylcholinesterase at 80 per cent. Both forms coexist, about equally, in amniotic membrane. A considerable arylesterasic activity is proved in cord, placenta and membrane, the greatest activity being in placenta. Comparing the greater activity in maternal plasma and cord blood's plasma to the very weak activity in amniotic fluid, it is possible to think that cork, membrane, placenta and also amniotic fluid pseudocholinesterase and arylesterase, come from plasma. On the contrary, placental acetylcholinesterase seems original and probably is the source of this enzyme activity in amniotic fluid.     

Self-Help Housing, the Poor, and the State in the Caribbean

Self-Help Housing, the Poor, and the State in the Caribbean. Robert B. Potter and Dennis Conway. eds. Knoxville: University of Tennessee Press, 1997. 299 pp.     

Selenium,cadmium, lead,and mercury concentrations in human breast milk,in placenta,maternal blood,and the blood of the newborn

The concentrations of the trace elements Cd, Hg, Pb, and Se during the perinatal period in human placenta and in the blood of the mother and the newborn (cord blood) were determined. Breast milk (colostrum and mature milk) was also included to permit correlations between the different compartments. For Cd, a placental barrier exists, in accord with previous observations. For Pb, a strong correlation between the concentrations in the blood of the mother and of the newborn was found. The concentration of Hg was in most cases below low the detection limit. Its concentration in colostrum was higher than in the mature milk. The results for Se reflect the knowledge about an essential trace element. Strong positive correlations were noted between maternal blood and cord blood and maternal milk. Anodic stripping voltammetry (DPASV) was used for the determination of Cd and Pb, cold vapor AAS (CVAAS) for the determination of Hg, and instrumental neutron activation analysis (INAA) for the determination of Se.     

Diacylglycerol, phosphatidic acid, and the converting enzyme, diacylglycerol kinase, in the nucleus

There exists phosphoinositide (PI) cycle in the nucleus, which is operated differentially from the classical PI cycle at the plasma membrane. Evidence has been accumulated that nuclear PIs and the related enzymes are closely involved in a variety of nuclear processes, although the details remain to be elucidated. In this mini review, some components of PI cycle, i.e., diacylglycerol, phosphatidic acid, and the converting enzyme, diacylglycerol kinase, in the nucleus are discussed with focusing on the lipid metabolism, cell cycle regulation, and animal models.     

Stress,adaptation, and speciation in the evolution of the blind mole rat,Spalax, in Israel

Environmental stress played a major role in the evolution of the blind mole rat superspecies Spalax ehrenbergi, affecting its adaptive evolution and ecological speciation underground. Spalax is safeguarded all of its life underground from aboveground climatic fluctuations and predators. However, it encounters multiple stresses in its underground burrows including darkness, energetics, hypoxia, hypercapnia, food scarcity, and pathogenicity. Consequently, it evolved adaptive genomic, proteomic, and phenomic complexes to cope with those stresses. Here I describe some of these adaptive complexes, and their theoretical and applied perspectives. Spalax mosaic molecular and organismal evolution involves reductions or regressions coupled with expansions or progressions caused by evolutionary tinkering and natural genetic engineering. Speciation of Spalax in Israel occurred in the Pleistocene, during the last 2.00–2.35 Mya, generating four species associated intimately with four climatic regimes with increasing aridity stress southwards and eastwards representing an ecological speciational adaptive trend: (Spalax golani, 2n = 54 → S. galili, 2n = 52 → S. carmeli, 2n = 58 → S. judaei, 2n = 60). Darwinian ecological speciation occurred gradually with relatively little genetic change by Robertsonian chromosomal and genic mutations. Spalax genome sequencing has just been completed. It involves multiple adaptive complexes to life underground and is an evolutionary model to a few hundred underground mammals. It involves great promise in the future for medicine, space flight, and deep-sea diving.     

Language, Ideology, and the Press in Catalonia

This article, relying on Bakhtin's approach to canonization, demonstrates an empirical link between language and ideology. The authors employ content and discourse analysis to examine political discourse exemplified in editorial writing as a particular speech genre. They scrutinize editorials in four newspapers published in Catalonia, two of them in Castilian and two in Catalan. Despite political differences between the papers in each language, the authors discern a Catalan-language discourse style that differs significantly from the Caslilian-language discourse style. They conclude that the historically canonized discourse styles, different in Catalonia's Castilian and Catalan press, reflect the intimate relationship between language of use and ideology.     

War, Factionalism, and the State in Afghanistan

Since September 11, 2001, the explanations offered to account for the rise of a foreign-led terrorist network on Afghan soil have variously focused on the political vacuum opened up by the Soviet withdrawal from Afghanistan in February 1989, interference by foreign powers in Afghanistan's internal affairs, the failure of Afghanistan to produce a "strong state" because of ethnic factionalism, and an internal moral incoherence inherent to Afghan culture. I argue that none of these explanations is entirely satisfactory in itself. To understand the situation in Afghanistan, we must recognize that its political and military chaos is not an isolated or unique phenomenon, and at the same time acknowledge the particular social and political dynamics of Afghanistan's history that have set the parameters for current events. I show that communal conflicts in Afghanistan are part of a much wider affliction common to many postcolonial states and multinational societies, and that Afghanistan's current situation can only be understood by focusing on its failed attempts at nation-state building within the broader geopolitical circumstance of foreign manipulation and proxy wars that have given rise to particular forms of ethnic division. [Keywords: Afghanistan, nation-building, ethnic factionalism, warfare, the State]     

Forces,fluctuations, and self-organization in the nucleus

We address several processes and domains in the nucleus wherein holding the perspective of physics either reveals a conundrum or is likely to enable progress.Like its predecessor in 2014, this second special issue of Molecular Biology of the Cell (MBoC) again engages the cell biology and biophysics community to envision the degree to which physics governs certain domains of cellular function.As early as a century ago, some leading physicists had envisioned the role their science could play in understanding living systems, as did Erwin Schrödinger with particular cogency (Schrodinger, 1944 ). Later, in the early 1950s, the physicist Francis Crick did a pioneering study in cell biology (probing the structure of cytoplasm by the resistance it offered to magnetic particles in an applied field) before turning his attention elsewhere (reviewed in Pederson, 2014 ). In a previous Perspective (Pederson and Marko, 2014 ), the broad outlines of the theme of physics were set forth as regards the nucleus. Here we refine those ideas, focusing on several open problems concerning how the nucleus operates and offering thoughts on how the perspective of physics has been—and will continue to be—useful as we fathom the nucleus.     

Selectivity, cooperativity, and reciprocity in the interactions between the delta-opioid receptor, its ligands, and G-proteins

A better understanding of signal transduction mechanisms is of critical importance. Methodologies that allow studies to be done while receptors are incorporated into lipid bilayers are advantageous. One such technique is plasmon-waveguide resonance (PWR) spectroscopy, which can follow changes in conformation accompanying protein-ligand, protein-protein, and protein-lipid interactions occurring in G-protein-coupled receptors in real time with high sensitivity and without the need for molecular labeling. Here we investigated several aspects of human delta-opioid receptor (hDOR)-G-protein interactions: 1) the effect of different types of agonists on the interaction with individual G-protein subtypes; 2) the affinities of the separate G-protein alpha and betagamma subunits to different ligand-occupied states of the receptor; and 3) the effect of the presence of the G-protein on the interactions of the ligand with the receptor. To accomplish this we have incorporated the receptor into a solid supported lipid bilayer in the presence of ligand or G-protein and monitored the PWR spectral changes induced by the reciprocal G-protein or ligand interactions. We found a high degree of selectivity in the interactions of different agonist-bound states of the receptor with the different G-protein subtypes. This has important implications for agonist-directed trafficking and selective drug design. Studies with the separated alpha and betagamma subunits show that cooperativity exists in these interactions. The high affinities of the separated subunits to the receptor point to the possibility of independent promotion of specific signaling events. The presence of G-proteins increased the affinity of agonists to the hDOR, and caused faster binding kinetics and different ligand-induced conformational changes. Because ligand also influences G-protein binding, reciprocity exists between these two binding processes.     

Bonding, biophilia, biosynergy, and the future of primates in the wild

Human and nonhuman primates bond with one another in countless ways, and the results are varied and vital to the individuals and species involved. The manifesto that is the basis for the collection of essays in which this commentary is included proposes that the "human/nonhuman bonds that arise in primatological research and practice deserve and demand study and research." An essential corollary of this proposal is that the primatologists themselves must be studied. The aim of this essay is to explore the influence of human/nonhuman primate bonding on conservation practice and on the future of primates in the wild. This commentary applies the author's professional experience as a conservation psychologist and his research on the impact of profound interspecies bonds on human worldviews, attitudes, and behavior. It examines two general categories of bonds: those driven by Biophilia (human fascination with life) and those influenced by Biosynergy (mutual enrichment of life). It is the author's premise that biosynergy promotes complex collaborative interspecies bonds that broaden the conservationist's desire to enhance synergy among all organisms in an ecosystem. Conversely, biophilia induces relatively simple unidirectional bonds between humans and other animals that deepen the conservationist's desire to understand and protect certain species. This contrast raises some crucial questions. Do biophilia-driven bonds between conservationists and their favorite primates blind them to the synergistic needs of all species and impair their ability to work for sustained preservation of threatened habitat? Does biosynergy-based human/nature bonding enhance focus on conservation as an ecological science and thus ignore species-specific factors crucial to assure survival of endangered primates? How can both types of bonds be optimally applied to the conservation of wildlife and wilderness?     

Fear, anxiety, and control in the zebrafish

Emotional responses are triggered by environmental signals and involve profound changes at multiple levels, from molecular to behavior. Much has been learnt about two emotions, fear and anxiety, by studying mammalian models. In particular, neural circuits and the corresponding molecular mechanisms essential for the learning and retention of fear, as well as the activation of anxiety, are well known. In contrast, little is known about how these emotions are terminated. The zebrafish is a newcomer to the world of emotion research. A number of assays for fear and anxiety now exist, but the underlying neural circuitry is largely undefined. Recent experiments, however, appear to provide a hint as to how anxiety is downregulated. In particular, they point to an essential role for a circuit involving the posterior septum, medial habenula, and interpeduncular nucleus. This evolutionarily conserved circuit may fulfill a similar function in mammals.     

Hypertension,Thrombosis, and Atherosclerosis in the Rat

A simple high fat diet containing cholic acid has been devised for producing hyperlipemia and an increased incidence of thrombosis in the small coronary vessels of the rat, but without producing significant atherosclerotic lesions. The influence on this syndrome induced by six weeks of desoxycorticosterone administration, 2 mg. daily, and 10 weeks of oral saline (1%) ingestion was investigated in 30 115-g. male rats. Marked hypertension developed only when it was induced prior to beginning the dietary feeding. In comparison to the control groups, the group that was both hyperlipemic and hypertensive had severer hypertension, severer hyperlipemia, double the mortality due to thrombosis and fatty streaks in the aorta but very few lesions of periarteritis nodosa. However, the early atherosclerotic lesions did not seem to be responsible for the increased production of thrombosis. It is therefore probable that under these experimental conditions hypertension has a more direct action on the production of thrombotic effect than that of worsening the atherosclerotic lesions.