共查询到19条相似文献,搜索用时 130 毫秒
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利用碳限制恒化实验研究了黑曲霉生长和糖化酶生产之间的相关性,结果表明当比生长速率低于0.068 h–1时,菌体生长与产酶是相关的,当比生长速率大于0.068 h–1时,菌体生长与产酶不相关。根据恒化实验结果获得黑曲霉葡萄糖底物消耗的Monod动力学模型,并结合葡萄糖和氧消耗的Herbert-Pirt方程和产物形成的Luedeking-Piret方程构建黑曲霉产糖化酶的黑箱模型。应用该模型设计指数补料分批发酵实验控制菌体比生长速率在0.05 h–1,使糖化酶的得率最高达到0.127 g糖化酶/g葡萄糖,并成功地使用模型描述了黑曲霉产糖化酶的发酵过程。实验值和模拟值进行比较表现出很好的适用性,表明黑箱模型可以用于指导黑曲霉产糖化酶发酵过程的设计和优化。 相似文献
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目的:研究蓝色犁头霉产壳低聚糖分批发酵动力学的模型.方法:在10L发酵罐中,将蓝色犁头霉分批发酵培养,对蓝色犁头霉菌丝体生长、产物形成和基质消耗的实验数据进行分析,根据Logistic和Luedeking-Piret方程分别建立蓝色犁头霉发酵过程菌体生长、壳低聚糖生成和基质消耗的动力学数学模型,并利用1stOpt软件对模型参数进行非线性曲线拟合.结果:研究得到了菌体生长、产物合成及基质消耗的动力学模型及参数,模型的拟合度分别为0.994、0.986、0.992.结论:研究表明蓝色犁头霉多糖合成和菌体生长呈生长偶联型,模型计算值与实验值有良好的拟合性,模型准确度较高. 相似文献
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TIAN Lv-ming YE Wei ZHAO Jie ZHU Chang-xiong YANG Hai-zhen LI pei YAO Juan GONG Da-chun 《工业微生物》2012,42(2)
研究了桔青霉发酵生产核酸酶P1的发酵动力学特性:以Logistic方程和Luedeking-Piret方程为基础,进行最优参数估计和非线性拟合,得到了描述整个发酵过程中的菌体生长、产物合成和基质消耗的动力学模型.对实验数据与模型预测值进行比较,发现模型预测值与实验数据能较好地拟合,基本上反映了桔青霉发酵过程的动力学特征,为以后进一步研究和预测核酸酶P1发酵过程奠定了理论基础. 相似文献
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以制备高产量的透明质酸酶为出发点,利用5 L发酵罐对球形节杆菌A152发酵生产透明质酸酶的条件进行优化,并对其发酵动力学模型进行研究。研究结果表明,转速400 r/min、通气量3.5 L/min时,生物量和透明质酸酶酶活力最优,分别为5 g/L、16.4 U/mL;同时对发酵过程中菌体生长、产物生成及基质消耗的规律进行研究,应用Logistic方程、Luedeking-Piret方程和底物消耗的物料平衡方程建立了球形节杆菌Arthrobacter globiformis A152发酵过程的动力学模型,并通过MATLAB软件进行最优参数估计和非线性拟合。模型的计算值与实验值能较好地拟合,表明所建的模型能较好地反映发酵过程,可为发酵过程的在线控制和预测提供理论基础。 相似文献
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通过三联30L全自动发酵罐对虾青素产生菌法夫酵母的分批发酵动力学进行了研究,结果表明,法夫酵母的生长与限制性基质葡萄糖浓度之间符合Logistic方程,建立了细胞生长、产物合成和基质消耗随时间变化的数学模型。应用MATLAB软件对发酵动力学模型进行最优参数估计和非线性拟和,获得最大比生长速率(umax)和产物得率(Yp/x)分别为0.1829/h、0.1524g/g,虾青素分批发酵中细胞生长与产物合成属于偶联型,模型模拟计算结果和实验值能较好地吻合,动力学研究结果表明该模型能较好地反映细胞的生长、底物消耗和产物合成过程机制。 相似文献
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放射形土壤杆菌Q9415发酵产胞外多糖动力学研究 总被引:2,自引:0,他引:2
放射形土壤杆菌Q9415产胞外多糖PS-9415的发酵为混合型发酵。应用logistic方程和L-P方程对PS-9415发酵过程的菌体生长,多糖合成以及底物消耗的动力学进行了讨论。研究发现:logistic方程和L-P方程建立的动力学模型较好地拟合了实验数据。 相似文献
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The effects of various metal ions on the simultaneous production of glucose oxidase and catalase by Aspergillus niger were investigated. Calcium carbonate induced synthesis of both enzymes. The induction of calcium carbonate was accompanied by a metabolic shift from the glycolytic pathway (EMP, Embden-Meyerhof-Parnas) to direct oxidation of glucose by glucose oxidase. The time course of the biosynthesis of both enzymes is reported. The logistic model was in good agreement with the experimental growth results. The production of both enzymes was growth-associated. Finally, a model of growth and product formation was also proposed. 相似文献
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模拟青霉素发酵过程中菌体生长动态的细胞自动机模型 总被引:4,自引:1,他引:3
在青霉素发酵生产机理及其动力学微分方程模型的基础上,建立了模拟青霉素分批发酵过程中菌体生长动态的细胞自动机模型(CABGM)。CABGM采用三维细胞自动机作为菌体生长空间,采用Moore型邻域作为细胞邻域,其演化规则根据青霉素分批发酵过程中菌体生长机理和动力学微分方程模型设计。CABGM中的每一个细胞既可代表单个的青霉素产生菌,又可代表特定数量的青霉素产生菌,它具有不同的状态。对CABGM进行了统计特性的理论分析和仿真实验,理论分析和仿真实验结果均证明了CABGM能一致地复现动力学微分方程模型所描述的青霉素分批发酵菌体生长过程。最后,对所建模型在实际生产过程中的应用问题进行了分析,指出了需要进一步研究的问题。 相似文献
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The production of gluconic acid, extracellular glucose oxidase and catalase in submerged culture by a number of biochemical mutants has been evaluated. Optimization of stirrer speed, time cultivation and buffering action of some chemicals on glucose oxidase, catalase and gluconic acid production by the most active mutant, AM-11, grown in a 3-L glass bioreactor was investigated. Three hundred rpm appeared to be optimum to ensure good growth and best glucose oxidase production, but gluconic acid or catalase activity obtained maximal value at 500 or 900 rpm, respectively. Significant increase of dissolved oxygen concentration in culture (16-21%) and extracellular catalase activity were obtained when the traditional aeration was employed together with automatic dosed hydrogen peroxide. 相似文献
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Batch fermentation kinetics of xanthan gum production from glucose by Xanthomonas campestris at temperatures between 22 degrees C and 35 degrees C were studied to evaluate temperature effects on cell growth and xanthan formation. These batch xanthan fermentations were modeled by the logistic equation for cell growth, the Luedeking-Piret equation for xanthan production, and a modified Luedeking-Piret equation for glucose consumption. Temperature dependence of the parameters in this model was evaluated. Growth-associated rate constants increased to a maximum at approximately 30 degrees C and then decreased to zero at approximately 35 degrees C. This temperature effect can be modeled using a square-root model. On the contrary, non-growth-associated rate constants increased with increasing temperature, following the Arrhenius relationship, in the entire temperature range studied. The model developed in this work fits the experimental data very well and can be used in a simulation study. However, due to the empirical nature of the model, the parameter values need to be reevaluated if the model is to be applied to different growth conditions. 相似文献
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《Journal of biological education》2012,46(4):195-200
Yeasts grown in anaerobic liquid media produced catalase in response to the presence of H2O2 in the growth medium. The fact that some of the induced enzyme was active at the cell surface, bound either to the cell wall or cell-surface membrane, eliminated the need to crush cells in order to release the enzyme complement. Instead, catalase production was monitored by using H2O2-reagent strips to detect changes in the level of H2O2, in the growth medium. In addition, catalase induction in yeasts was found to be temperature-sensitive. It is suggested that biology teachers in schools might find the following experiments useful for demonstrating essential features of substrate-induced enzyme synthesis, based on the Jacob-Monod model, and for showing that the activity of certain genes can be modified by environmental temperature. 相似文献
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AIMS: Using limited digital image sampling, a model of fungal growth in soil that considers both hyphal production and lysis was constructed for two strains of Trichoderma virens over a range of four temperatures. MATERIALS AND METHODS: A growth model was developed by fitting the radial cross sectional data with a modified form of the Ratkowsky equation to determine maximum growth rate and a modified Arrhenius equation to determine maximal rate of decrease in area covered by mycelia. The parameters obtained from a combined equation were then verified by using the data obtained from the whole colony to determine the appropriateness of the model. CONCLUSIONS: Using a limited data set and a combination of the Ratkowsky and Arrhenius equations, the mycelial coverage of the T. virens colony was determined, relating microscopic hyphal growth to macroscopic colony growth. This model was sufficiently robust to predict growth across four temperatures for a genetically modified and wild-type strain of T. virens. SIGNIFICANCE AND IMPACT OF STUDY: By using simple assumptions for the increase and eventual decline in fungal growth on a resource-limited medium, this model constructs an initial framework onto which additional parameters such as nutrient consumption could be incorporated for prediction of fungal growth. 相似文献
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Fiedurek J Gromada A Słomka A Korniłowicz-Kowalska T Kurek E Melke J 《Acta biologica Hungarica》2003,54(1):105-112
The total of 98 strains of moulds were isolated from soils collected in arctic tundra (Spitzbergen). Among these strains Penicillium cyclopium 1, the most effective for production of catalase, was selected by the method of test-tube microculture. The time course of growth and catalase production by this strain showed the intracellular activity of this enzyme to be about 3-fold higher than its extracellular level Some properties of crude catalase preparation, isolated from postculture liquids by lyophilization, were also examined. Catalase activity showed its maximum at 15 degrees C, indicating adaptation of the enzyme to lower temperatures of the arctic environment. 相似文献
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一株嗜热子囊菌产生的碱性耐热过氧化氢酶及其应用潜力 总被引:11,自引:0,他引:11
研究了一株嗜热子囊菌产过氧化氢酶的摇瓶发酵条件,并对其在纺织工业中的应用潜力进行了评价。以20 g/L糊精和1%(V/V)乙醇为混合碳源时,过氧化氢酶酶活达到1594 u/Ml,比以糊精和乙醇单独为碳源时过氧化氢酶的活力之和还高23%。改变培养基的初始Ph、提高发酵液中的溶氧水平及添加外源过氧化氢,过氧化氢酶的产量进一步提高到2762 u/Ml,比优化前提高了5.8倍。将嗜热子囊菌的过氧化氢酶同来源于牛肝、黑曲霉的过氧化氢酶进行了热(70℃, 80℃, 90℃)、碱(Ph 9.0, Ph 10.0, Ph 11.0)稳定性的比较。结果显示,产自嗜热子囊菌的过氧化氢酶对高温和强碱性的耐受性能明显优于其它来源的酶,在纺织染整工艺中具有良好的应用潜力。 相似文献
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A new generalized mathematical model for recombinant bacteria which includes inducer effects on cell growth and foreign protein production is developed. The model equation set was applied to a host-vector system, Escherichi coli D1210 and plasmid pSD8. Batch experiments were designed and performed in shake flasks to verify the model. A parameter estimation method was developed and proven to be efficient. Although simple, the model can effectively describe the dynamics of the production of foreign protein in recombinant bacteria and can be used for optimization and control studies to maximize foreign protein production. 相似文献