Isolation of xyloglucans from etiolated Glycine max and Vigna sesquipedalis hypocotyls

Xyloglucans isolated from cell walls of etiolated Glycine maxand Vigna sesquipedalis hypocotyls were subjected to fragmentationanalysis with cellulase for structural comparison with thosederived from Phaseolus aureus hypocotyls. The xyloglucans fromG. max and V. sesquipedalis had glucose, xylose, galactose andfucose in the approximate molar ratio of 10:6:4:1 and 10:7:3:1,respectively. However, the results of cellulase fragmentationanalysis of xyloglucans from the three species suggested thatthe basic structure of the xyloglucans in the cell walls ofthese bean-hypocotyls is almost the same; the structure is basedon two repeating oligosaccharide units, one of which consistsof glucose and xylose and the other of glucose, xylose, galactoseand fucose. ¹ Present address: Toppan Printing Co., Ltd., Okaji, Sendai980, Japan. (Received February 3, 1977; )     

Role of Cell-wall-degrading Enzymes in the Development of Leaf Spots Caused by Ascochyta pisi and Mycosphaerella pinodes

Extracts of limited and spreading lesions caused by Mycosphaerellapinodes on detached pea leaflets contained proteolytic, cellulolytic,and pectolytic enzymes although only in spreading lesions wasthere much degradation of cell walls. The brown tissue fromlimited M. pinodes lesions was resistant to maceration by enzymesfrom spreading lesions. Limited lesions contained water-soluble,95 per cent ethanol insoluble, partially dialysable, inhibitorsof pectin transeliminase which is probably the macerating enzyme. Green, spreading M. pinodes lesions developed only on leafletsfloating on water. Growth of these lesions was accompanied bycontinous loss of phenolic substances to the water while thephenol content in infected tissue remained similar to that inuninoculated controls. In contrast, the phenol content in mature,limited M. pinodes lesions on leaflets suspended just abovethe water level was about four times that in healthy tissue.It is suggested that loss of phenolics from floating leafletsprevents tissue browning and the development of resistance ofthe cell walls to maceration. But this type of resistance doesnot appear to be a major factor in the limitation of lesionson suspended tissue. Extracts of limited Ascochyta pisi lesions on leaflets floatingon water contained pectolytic and hemicellulolytic enzymes.Some cellulase (Cx) activity was detected although there waslittle evidence of cellulose degradation in cell walls in infectedtissue. The nature of the macerating factor remains uncertainbut it was found that extracts from lesions contained inhibitorsof pectic enzymes and that tissue just beyond that colonizedby the fungus was resistant to maceration; this resistance isprobably important in restricting the growth of the pathogenin the leaf.     

Predicted Patterns of Photosynthetic Production in Maritime Antarctic Mosses

The microclimate of a moss turf of Polytrichum alpestre Hoppeand a moss carpet of Drepanocladus uncinatus (Hedw.) Warnst.is described for two sites in the maritime Antarctic. Thesemicro-climatic data, together with some additional data, arethen used in the solution of multiple regression equations describingnet photosynthetic response to variation in radiant flux densityand temperature as measured in Antarctic material of the twomosses grown under controlled conditions in the U.K. Predictionsare made of daily and seasonal courses of net photosynthesisat the Antarctic field site. The basis of acclimation of netphotosynthesis to growth temperatures through a season is discussedand the physiological basis of growth under snow is examined. Polytrichum alpestre. Hoppe, Drepanocladus uncinatus (Hedw.) Warnst., mosses, Bryophyta photosynthesis, productivity, Antarctic     

Tetraphis browniana var. repanda (Funck) Hampe—New to the British Isles

Abstract

Syrrhopodon spiculosus Hook. &; Grev. and some associated taxa are revised. Syrrhopodon elimbatus Dixon and Syrrhopodon patens Dixon are confirmed as synonymous with S. spiculosus; Syrrhopodon hispidissimus Dixon and Syrrhopodon scalariformis Dixon are recognized as distinct species; S. spiculosus ssp. bokorenis Tixier is recognized as a synonym of S. mammillosus Müll.Hal. and Syrrhopodon perarmatus Broth. in Broth &; Watts is placed in synonymy with Syrrhopodon subciliatus Müll. Hal. Two new species are described from Borneo: Syrrhopodon teniolatus L.T.Ellis, sp. nov., which resembles S. hispidissimus but has a differentiated rib (teniola) within the margin of the distal hyaline leaf base, and Syrrhopodon multipapillosus L.T.Ellis, sp. nov. This resembles a miniature version of Syrrhopodon kilauae Müll.Hal. but has cells in the chlorophyllose lamina exserted dorsally and/or ventrally as tall, papillose projections.     

Variation in Syrrhopodon armatus Mitt. (Calymperaceae), and in closely related taxa from Madagascar and adjacent regions

The range of variation in the features of Syrrhopodon armatus Mitt. is discussed, and the interrelationships of this species with Syrrhopodon armatissimus W.D.Reese and Syrrhopodon armatus ssp. insularus (Bizot & Onr.) Orbán & W.D.Reese are assessed. The latter taxon is reinstated at the level of species. A key to distinguish between these taxa is provided.     

Characterization of the Hydrolytic Activity of Avocado Cellulase

The cellulase produced by ripening avocado fruits (Persea americanaMill cv. Fuerte) was isolated and purified using chromatofocusing(pH 7–4) and gel filtration on a Bio- Gel P-100 column.Characteristics of the cellulase were assessed by using, assubstrates, a range of polysaccharides containing various sugarresidues and varying types of linkages between the residues.Only those substrates containing (14)-ß-glucosyl linkageswere hydrolyzed by the purified enzyme. Two polysaccharidesthat were extensively hydrolyzed by the cellulase were carboxymethylcelluloseand (13),(14)-ß-D-glucans such as from Avena endospermcell walls. Characterization of the activity in the degradationof the mixed linked glucan of Avena and cellodextrins indicatedthat the enzyme has a limit recognition-hydrolytic site of four(l4)-ß linked glucose residues. It was also foundthat the enzyme could cleave only (14)-ß-linkagesthat were adjacent to other (l4)-ß-D-glucosyl linkages.Activity of the cellulase against isolated avocado fruit cellwalls indicated that the purified enzyme was incapable of appreciablysolubilizing the cellulosic components of these walls. ¹Supported in part by National Science Foundation Research GrantPCM 7818588. ²USDA-ARS, Dairy Forage Research Center, University of Wisconsin,Madison, WI 53706. ³Department of Vegetable Crops, University of California, Davis,CA 95616. (Received September 14, 1985; Accepted February 12, 1986)     

Syrrhopodon spuriodisciformis Dusén (Calymperaceae) as a distinct species,with taxonomic notes on Syrrhopodon africanus (Mitt.) Paris and related taxa

Syrrhopodon spuriodisciformis Dusén is removed from synonymy with Syrrhopodon africanus (Mitt.) Paris and reinstated as a distinct species. Syrrhopodon guineensis Broth. & Paris is recognised as a synonym of S. spuriodisciformis. Syrrhopodon africanus ssp. graminicola (R.S.Williams) W.D.Reese and S. africanus ssp. mandrakensis (Tixier) W.D.Reese are reconsidered as species, and the status of Syrrhopodon obuduensis Egunyomi & Olar. is discussed.     

Syrrhopodon cuneifolius Thér. (Calymperaceae), a good species endemic to Madagascar,with notes on S. leprieurii Mont. from the American tropics

Syrrhopodon cuneifolius Thér. is lectotypified and confirmed as a good species, endemic to Madagascar. The features distinguishing it from the superficially similar Syrrhopodon leprieurii Mont., and morphological variation in the latter are discussed. In contrast to the leaves in S. leprieurii, those in S. cuneifolius generally lack large spines, possess a costa with a ventral cortex of small pluripapillose cells, and have a marginal stereome that fails short of the leaf apex. Consideration is given to removing Syrrhopodon recurvulus Mitt. from synonymy with S. leprieurii and placing it in synonymy with Syrrhopodon gaudichaudii Mont.     

Anatomy of Ricciocarpus natans (L.) Corda, Studied by Scanning Electron Microscopy

The anatomy of the vegetative thallus in the life-forms of theamphibious liverwort Ricciocarpus natans was studied by scanningelectron microscopy. Particular attention was given to the structureof the epidermal stomatoid pores and the minute internal poresin the aerenchyma. The aquatic and terrestrial life-forms werefound to differ in (1) number and size of ventral scales, (2)presence of rhizoids and (3) width of the thallus margin. scanning electron microscopy, Ricciocarpus natans (L.) Corda, Bryophyta, liverwort, stomatoid pores, aerenchyma, structural adaptations     

A revised synonymy for Syrrhopodon trachyphyllus (Calymperaceae,Musci) and some related old world taxa

Several taxa erroneously regarded as conspecific with Syrrhopodon trachyphyllus Mont. are reinstated. These include Syrrhopodon cavifolius Sande Lac., S. planifolius P. de la Varde, S. semperi Müll. Hal., S. tosaensis Cardot and Calymperopsis katemensis Zanten (=Syrrhopodon katemensis (Zanten) L.T. Ellis, comb. nov.). Syrrhopodon ludovicae Broth. & Paris is recognized as a synonym of S. mammillosus Müll. Hal., Syrrhopodon orientalis W. D. Reese & P. J. Lin as conspecific with S. cavifolius Sande Lac., Syrrhopodon cambodiensis Tixier as synonymous with S. semperi, S. calymperoides Cardot & P. de la Varde as conspecific with Syrrhopodon prolifer Schwägr., and S. prolifer var. mossmanensis W. D. Reese is placed in synonymy with S. katemensis (Zanten) L.T. Ellis. Varieties of Syrrhopodon prolifer Schwägr. occurring in the palaeotropics are discussed.     

Cellulase, Fruit Softening and Abscission in Red RaspberryRubus idaeusL. cv Glen Clova

The ripening of raspberry fruit (Rubus ideausL. cv Glen Clova)is associated with a climacteric rise in ethylene production.As the fruit pigments change from green to red there is a progressivesoftening, loss of skin strength and a breakdown of cell wallsin the mesocarp. An increase in cellulase (endo-1,4-ß-D-glucanase)in both drupelets and receptacles accompanies these changes.The localization of cellulase in the regions of the fruit associatedwith abscission zones suggest the enzyme may be involved infruit separation as well as softening. Rubus idaeusL; raspberry; fruit ripening; ethylene; abscission; cell wall breakdown; cellulase; endo-1,4-ß-D-glucanase     

麦长管蚜唾液中几种酶的鉴定、活力测定与功能分析

用Parafilm膜夹营养液法,以两种食料介质饲喂麦长管蚜Macrosiphum avenae 3龄若蚜并收集其唾液,对唾液中的酶类进行了鉴定、活力测定和功能分析。结果表明,在20%蔗糖介质提取液中,鉴定有果胶酶、多酚氧化酶和纤维素酶; 在水介质提取液中鉴定有纤维素酶; 两种介质提取液中都未鉴定出过氧化物酶。酶活力测定结果表明, 在20%蔗糖介质提取液中, 每30头蚜虫分泌的果胶酶、多酚氧化酶和纤维素酶的酶活力分别为2.59×10^-3 U/g、7×10^-3 U/g和7.89×10^-3 U/g; 在水介质提取液中,纤维素酶活力为3.68×10^-3 U/g。行为反应试验结果表明,果胶酶处理麦苗的挥发物组分能引起麦长管蚜寄生性天敌燕麦蚜茧蜂Aphidius avenae和捕食性天敌七星瓢虫Coccinella septempunctata 的嗅觉偏好反应,因此,果胶酶在麦长管蚜取食诱导小麦植株的间接防御反应中具有重要作用。     

Enzymatic Isolation of Protoplasts from Fern Protonemal Cells Stainable with a Fluorescent Brightener

Protoplasts were successfully isolated for the first time fromthe filamentous protonemal cells of ferns after the cells werecultured in contact with both air and medium. Sterilized sporesof Adiantum capillus-veneris and Pteris vittata were inoculatedon a piece of nylon mesh (40- {diaeresis}m mesh) placed on amat of polyester fibers which was soaked in liquid culture medium,and the spores were illuminated from above with continuous redlight. Protonemal cells, exposed to the air during this procedure,could be stained with Calcofluor White, a dye that binds tocell walls. Protoplasts were easily isolated from these protonemalcells by digestion of the cell wall with cellulase and pectinase.A total of 0.8–1.9 x 10⁴ and 0.6–2.0 x 10⁴ protoplastswere obtained from protonemata that originated from 10 mg ofdry spores of Adiantum and of Pteris respectively. Viability,as judged by staining with fluorescein diacetate was more than90% for both species. Staining with 4'6-diamidino-2-phenylindole(DAPI) revealed that about half of the protoplasts of both speciescontained a nucleus. (Received May 22, 1989; Accepted September 5, 1989)     

Characterization of a Non-abscission Mutant in Lupinus angustifolius L.: Physiological Aspects

A single-gene recessive mutant (Abs^-) of Lupinus angustifoliusL. ‘Danja’ that does not abscise any organs wascompared with its parent during continuous exposure of explantsfrom 14 d old seedlings to 10 µl l^-1ethylene. Both endo-(1,4)-ß- D -glucanase (cellulase) and polygalacturonase(PGA) activities increased significantly and progressively inpetiole-stem abscission zones of the parent before the onsetof abscission, and were reflected in a rapid decline in breakstrengthfrom 300 to 70 g within 32 h. In the mutant there was negligibleincrease in hydrolytic enzyme activity, breakstrength declinedslowly (to 180–200 g by 72 h) and there was no abscission.Isoelectric focusing showed two cellulase isoforms (pI 5.0 andpI 8.5) expressed in abscission zones of the parent; these wereexpressed at much lower levels in the mutant. These data areinterpreted to indicate that expression of at least two formsof cellulase activity is enhanced by ethylene in normal petioleabscission zones of lupin. PGA activity also increased in theabscission zone tissue of the parent but to a lesser extentin that of the mutant. We attribute the Abs^-phenotype to mutationof a gene regulating ethylene-responsive expression of abscission-specifichydrolytic enzymes. Copyright 2001 Annals of Botany Company Lupinus angustifolius, abscission, breakstrength, cellulase, ethylene, legume, lupin, mutant, polygalacturonase     

Isolation of Protoplasts from Kappaphycus alvarezii var. tambalang (Rhodophyta) and Secretion of i-Carrageenan Fragments by Cultured Cells

A method for generating protoplasts from the carrageenan-producingred alga Kappaphycus alvarezii was developed. Digestions withcellulase and _k-carrageenase produced only a few cortical cellprotoplasts, while digestions with cellulase and i-carrageenaseonly produced epidermal cell protoplasts. When both carrageenaseswere used in the digestion media with cellulase, protoplastswere released from all cell types and yields ranged from 1·0to 1·2x10⁷ cells g^–1 with sizes from 5 to 200 µmdiameter. Protoplasts were subsequently cultured to study cellwall regeneration. Calcofluor-positive material (probably cellulose)was detected within 6 h after removal of protoplasts from thewall digestion media, whereas, i-carrageenan fragments weredetected in all regenerating protoplast cultures 24 h afterremoval from the digestion media. Protoplasts continued to produceCalcofluorpositive material and secrete carrageenan fragmentsinto culture media for several days. However, cells culturedin media augmented with K⁺ ions stopped secreting carrageenanfragments after 24 h. Cells cultured for 48 h in seawater labelledweakly with an i-carrageenan hybridization probe, but not atall with a corresponding _k-probe. Cells cultured for 48 h, blottedto nylon membranes and probed with anti-carrageenan monoclonalantibodies, showed the presence of gelling carrageenan subunitsin the cell walls. Key words: -Carrageenan, Kappaphycus, protoplasts, Rhodophyta     

Flower-Inducing Activity of Water Extracts of Various Plant Species, in Particular Pharbitis nil

The crude water extracts of leaves of many plant species belongingto Spermatophyta and some belonging to Bryophyta induced floweringof Lemna paucicostata 151 (PI51) under continuous light, atthe concentrations equivalent to 0.1 to 10 mg fr wt leaf per10 ml culture medium (mg fr wt/10 ml). The extract of Salvinia(Pterydophyta) added together with the extract of Lemna at aconcentration lower than that necessary to cause flowering alsoinduced flowering. The activity of the water extracts of someplants varied considerably from experiment to experiment dueto unknown factors, but the extracts of Pharbitis nil strainViolet, a sensitive short-day plant, always showed a high activity,as did the extracts of Lemna paucicostata reported previously. The extract of Pharbitis cotyledons induced flowering of P151even at 0.3 mg fr wt/10 ml, and significantly promoted floweringof L. paucicostata 441 and 6746 at 1–3 mg fr wt/10 ml.Ex-udate from the cuttings of the seedlings was also active.However, neither the activity of the water extract nor thatof the exudate could be correlated with photoperiodic floralinduction. On the other hand, the extract of leaves or cotyledonshad higher activity (on a fr wt basis) than that of other organs,and the activity of the extract of cotyledons changed with theirage roughly in parallel with their photoperiodic sensitivity. (Received April 17, 1989; Accepted August 10, 1989)     

A Survey of the Rates and Products of Short-term Photosynthesis in Plants of Nine Phyla

1. Short-term photosynthetic experiments using C¹⁴O₂ and paperchromatography were performed with 27 different plants representingnine phyla: Schizophyta (Schizophyceae), Euglenophyta, Chlorophyta,Charophyta, Chrysophyta, Rhodophyta, Bryophyta, Pteridophyta,and Spermatophyts.
2. There is a remarkable uniformity in thetypes of ethanol-solublecompounds which became radioactivein the entire group of plantsused. The amounts of the differentcompounds varied considerablypercentagewise among the variousplants as would be expectedbecause of their inherent metabolicdifferences and the variationsin their physiological statesinduced by experimental conditions.
3. Sucrose became radioactivein very different amounts in twomajor groupings of plants:(a) those containing only photosynthetictissue, and (b) thosecontaining non-photosynthetic tissue aswell. The amount ofradioactive sucrose in the former groupwas much lower thanthat in the latter.
4. An unidentified compound became radioactivein appreciable amountsin two of the blue-green algae, but wasradioactive in verysmall amounts or not visible at all on thechromatograms ofall other plants.

     

Viscosimetric determination of cellulase activity: critical analyses

The mode of expression of cellulase activity obtained by viscosimetricmeasurement is analysed. After testing different types of substrates,it appears that the best one is hydroxyethylcellulose used ata high degree of polymerisation and a high concentration. Comparisonof results obtained with cellulases from Trichoderma virideand extracted from Pisum sativum favours the validity of thedetermination proposed. Possible physiological significanceof the measurements of cellulase activity is also discussed. (Received February 10, 1976; )     

The Bryophyta (Mosses): Systematic and Evolutionary Inferences from an rps4 Gene (cpDNA) Phylogeny

Phylogenetic analyses of nucleotide and amino acid sequencesof the chloroplast protein coding generps4 were performed for225 species of mosses, representing 84% of families recognizedby Vitt (1984. In: Schuster RM, ed. New manual of bryology,vol 2. Nichinan: Hattori Botanical Laboratory), under the criterionof maximum parsimony with Takakia and Sphagnum as outgroups.Most parsimonious topologies converge to a scenario whereinthe Andreaeidae are monophyletic and sister to the Bryidae (peristomatemosses), the Nematodonteae and the Buxbaumiaceae form a monophyleticlineage, the Diphysciaceae are sister to the Arthrodonteae and,within the latter, the Funarineae-Encalyptineae-Timmiaceae-Haplolepideaecompose a monophyletic clade sister to remaining diplolepideousmosses. This hypothesis suggests that early in the evolutionof the Arthrodonteae, two major lineages diverged, with oppositeand alternate peristomes, respectively. Bootstrap support forthe deep dichotomies is poor or lacking but increases when proteintranslations ofrps 4 sequences are included in the analysis.Several novel systematic hypotheses are raised, including (a)a diplolepideous rather than haplolepideous origin of the Pleurophascaceae;(b) an affinity of the Catascopiaceae with the Funariineae ratherthan the Bryineae; and (c) a close relationship of the Calomniaceaeand Mitteniaceae to the Rhizgoniaceae. The advantages and disadvantagesof a single gene phylogeny are discussed with respect to theidentification of polyphyletic familial or suprafamilial taxa.Copyright 2001 Annals of Botany Company Bryophyta, mosses, phylogeny, rps4, evolution, peristome, parsimony, sequences     

Endoreduplication Occurs during Pod Wall Development in Temperate Grain Legumes

Differentiation to a specialized function in plant tissue isassociated with an increase in the DNA content of the cellsover the diploid state. Using flow cytometry, ploidy levelswere assessed during pod development in three white lupin genotypesunder three environmental conditions and in other lupin speciesand temperate grain legumes in one environmental condition.Endoreduplication was found in the pod walls of all genotypesstudied. Higher ploidy levels coincided with maximal growthof the pod. DNA replication was not related to the proportionof the pod that comprised walls. Endoreduplication also occurredin other temperate grain legumes with at least two DNA duplicationsduring the development of the pod walls. The biological significanceof endoreduplication in the pod walls of grain legumes is unknown,but could be related to the storage function of these organs.Copyright2000 Annals of Botany Company Lupinus species, Pisum sativum, Phaseolus vulgaris, Vicia faba, pod wall development, ploidy level, flux cytometry, Bis-benzimide Hoechst 33258