Distribution of 5-doxylstearic acid in the membranes of mammalian cells

Concentration-dependent spin broadening of ESR spectra of the nitroxide 5-doxylstearic acid has been used to evaluate the distribution of 5-doxylstearic acid in the membranes of intact mouse thymus-bone marrow (TB) and Chinese hamster ovary (CHO) cells. TB cells, CHO cells, erythrocytes, and isolated plasma membranes from CHO cells were labelled with 5-doxylstearic acid and the peak to peak linewidths of the central line of the resulting ESR spectra were measured. The measured line widths were linearly dependent on the amount of 5-doxylstearic acid incorporated into the sample over the range of 0-0.18 mol nitroxide per mol lipid. In erythrocytes, the relationship between linewidths approximated a linear function at lower concentrations of 5-doxylstearic acid, up to 0.07 mol nitroxide per mol lipid. The amount of broadening of the central line for a given amount of 5-doxylstearic acid was far less for intact cells than for either erythrocytes or plasma membrane, indicating that the 5-doxylstearic acid samples a much larger lipid pool in the intact cells. With the broad assumption that the mobility of the 5-doxylstearic acid is similar in different membranes, the size of the lipid pool sampled by 5-doxylstearic acid is approximately equal to the total cellular lipid in intact cells. If a given concentration of 5-doxylstearic acid sampled only the plasma membrane of TB or CHO cells, we would expect to see a linewidth corresponding to a 12-20-fold greater local concentration of 5-doxylstearic acid than was observed, since the plasma membranes of CHO and TB cells represent only 5-8 percent of the total cellular lipid. Therefore, the 5-doxylstearic acid must distribute into most or all cellular membranes of intact cells and is not localized in the plasma membrane alone.     

Investigation of the mechanisms for stark broadening and shift of the spectral lines in a nonideal plasma

Abstrac A method for calculating the Stark broadening and shift of the spectral lines was developed based on a combination of the ion plasma model and new theoretical and computational methods for taking the Stark effect into account. Theoretical results are compared with experimental data on the broadening and shift of the spectral lines in a strongly nonideal plasma. __________ Translated from Fizika Plazmy, Vol. 30, No. 10, 2004, pp. 944–947. Original Russian Text Copyright ? 2004 by Denisov, Orlov, Fortov, Kulish, Gryaznov, Mintsev.     

Hydrogen lines in the infrared region and spectral background for the thomson scattering diagnostics of the iter divertor plasma

Calculations are made of the plasma spectral background, which is important for the Thomson scattering diagnostics in the ITER divertor. Theoretical grounds have been elaborated for computing the hydrogen spectral line shapes in the infrared spectral region for a divertor plasma in ITER. The shape of the P-7 Paschen line (transition n = 7 → n = 3) located near the laser scattering signal has been calculated for various lines of sight in the ITER divertor. Contributions from different mechanisms of broadening the P-7 line have been examined. The spectral intensities of bremsstrahlung and photorecombination continuum have been calculated. All calculations use data on the spatial distribution of temperatures and densities of all species of plasma particles computed with the SOLPS4.3 code for basic operation regimes of the ITER divertor.     

The effect of charge exchange with neutrals on the saturation of the spectral line intensities of multicharged ions in plasma

The influence of charge-exchange processes on the spectral line intensities of impurity ions in the edge and core plasmas of fusion devices is considered. It is found that, at a sufficiently high density of neutrals, the rate at which the atomic states are populated through charge exchange becomes independent of the neutral density, which results in the saturation of the spectral line intensities. This effect can substantially limit the efficiency of impurity-ion spectroscopy. Conditions under which the saturation effect manifests itself are examined for both the edge and core plasma regions in the presence of fast neutral beams. The results of calculations for the edge plasma are used to interpret the experimental data from the TORE SUPRA tokamak. It is shown that, in the central plasma region, the intensities of the visible spectral lines associated with the charge exchange of impurity ions in the course of neutral beam injection decrease (rather than increase, as was expected earlier) with increasing ion charge.     

Study of the emission characteristics of a low-pressure discharge in crossed E × H fields by using an echelle spectrograph with compensated astigmatism

In order to prove that thermal ionization is the main mechanism for sustaining a high-current low-pressure diffuse discharge in crossed E × H fields, the ion temperature was estimated by analyzing the Doppler broadening of a spectral line. The emission spectrum from discharge plasma was recorded using a specially designed echelle spectrograph with compensated astigmatism and with a resolution of 0.02 nm.     

Charge-exchange recombination spectroscopy of the plasma ion temperature at the T-10 tokamak

Charge-exchange recombination spectroscopy (CXRS) based on a diagnostic neutral beam has been developed at the T-10 tokamak. The diagnostics allows one to measure the ion temperature profile in the cross section of the plasma column. In T-10 experiments, the measurement technique was adjusted and the elements of the CXRS diagnostics for ITER were tested. The used spectroscopic equipment makes it possible to reliably determine the ion temperature from the Doppler broadening of impurity lines (helium, carbon), as well as of the spectral lines of the working gas. The profiles of the plasma ion temperature in deuterium and helium discharges were measured at different plasma currents and densities, including with the use of active Doppler measurements of lines of different elements. The validity and reliability of ion temperature measurements performed by means of the developed CXRS diagnostics are analyzed.     

Distribution of the coulomb microfield inside an ion cluster

The microfield distribution function in clusters is investigated numerically by the molecular dynamics and Monte Carlo methods. The results obtained are compared with the microfield distribution in an unbounded plasma. The calculated distributions are shown to have the same asymptotic forms. The position of the maximum and the existence of additional extrema depend on the size and type of cluster. The dependence of the probabilistic average and dispersion of the microfield distribution function on the number of particles in the cluster is studied.     

Evaluation of the ion temperature profile from measurements of the DD fusion products in the T-10 tokamak

Results are presented from measurements of the energy spectra and fluxes of the escaping charged fusion products and fluxes of fusion neutrons in ohmic regimes of the T-10 tokamak. The central temperature of the plasma ions is determined from the broadening of the energy spectra of thermonuclear protons and tritons. The ion temperature profile is evaluated from the dependence of the fluxes of charged fusion products on the radial plasma shift. It is proposed to use a single spectrometric detector with several collimators and slowing-down foils to measure the distribution of charged fusion products over pitch angles, which permits the determination of the ion temperature profile in a single shot. The feasibility of the method proposed is proved experimentally.     

Determination of the plasma density in a current sheet from the profiles of the HeII 6560-Å and Hα spectral lines

The electron density in the midplane of a current sheet and far from it is determined from an analysis of the profiles of the HeII 6560-Å and H_α spectral lines. A new approach to calculating the Stark broadening of the HeII 6560-Å line is developed. The results obtained can be used to determine the plasma density in other experimental devices.     

Suprathermal plasma flows in current sheets formed in two- and three-dimensional magnetic configurations

Dynamics of the thermal and directed motions of argon plasma ions in current sheets formed in various magnetic configurations was investigated experimentally Measurements in three-dimensional magnetic configurations with an X line were carried out for the first time. The results of these measurements were compared with the data obtained in experiments with two-dimensional magnetic configurations. The ion temperature and the energies and velocities of directed plasma flows within the current sheet were determined by analyzing the shapes of argon ion spectral lines broadened due to the Doppler effect. It is found that, under the given experimental conditions, the axial magnetic field does not affect the ion temperature and plasma acceleration in the sheet.     

Microfield model of quasi-independent particles and nonideal plasma

An original model of a plasma microfield constructed from first principles is much more advantageous than the earlier ones. The validity of the model is confirmed by optical measurements. The thermodynamics of a gaseous plasma is qualitatively described by a new ionization equilibrium model in which the interaction between charged particles is accounted for by means of a self-consistent plasma microfield. Numerical simulations show that, in this new model, nonideal effects are insignificant even at high plasma densities, and there are no phase transitions in a gaseous plasma, a conclusion that agrees with experimental data. The model is tested against the popular models of nonideal plasma and the SESAM database.     

Diagnostics of a current-sheet plasma with the use of helium spectral lines with forbidden components

Results are presented from the first measurements of the profiles of the HeI 447.1-nm and HeI 492.2-nm neutral helium spectral lines emitted by the plasma of a current sheet formed in the CS-3D experimental device. A theoretical analysis of these profiles is performed with the model microfield method. A comparison of the theoretical and experimental profiles shows that the electron density in the peripheral regions of the current sheets amounts to (1.0–2.0)×10¹⁵ cm^?3.     

Manifestation of the structural phase transitions of lipid bilayers in the ESR spectrum of s-ions in the electron state

The mechanism of S-ion line width for the tetrahonal cubic complex which was formed by lipids of the lipid bilayer at phase transition is considered. Within the framework of the one-dimensional model of domain walls or kinks created in the phase separation regions of the bilayer the spectral density of crystal field fluctuation of S-ion by radial distortion of the paramagnetic complex is calculated. If the modulation frequencies of an axial component of the crystal field are near the two-fold frequency of ESR, it is possible to reveal the characteristic band of narrowing and broadening of ESR line width for binding S-ions in the bilayer. The proposed theoretical model can be useful in the explanation of experimental values of ESR line width of S-ions binding in plankton. Characteristic parameters of kinks are estimated.     

Monoanion inhibition and 35Cl nuclear magnetic resonance studies of renal dipeptidase.

Kinetic analyses of monoanion inhibition and 15Cl nuclear magnetic resonance at 5.88 MHz were employed to study monoanion interactions with the zinc metalloenzyme, renal dipeptidase. The enzyme-catalyzed hydrolysis of glycyldehydrophenylalanine exhibited competitive inhibition when the reaction rate was determined in the presence of the monovalent anions fluoride, chloride, bromide, iodide, azide, nitrate, or thiocyanate or upon the addition of the divalent anion, sulfate. Competitive inhibition was produced by these anions. One anion was bound per enzyme molecule, and except in the case of fluoride all of the anions appeared to bind at the same site. Cyanide ion produced a much more effective inhibition of renal dipeptidase than the other monoanions, and it was shown that two cyanide ions were bound per enzyme molecule. An investigation of the effect of pH upon monoanion inhibition suggested that the anion inhibitors bind to the group with a pK of approximately 7.8. Complete dissociation of this group (approximately pH 8.4) eliminates the inhibitory effect of anions. The 35Cl line broadening produced by renal dipeptidase in 0.5 M NaCl solutions was 100 times more effective than that produced by equivalent concentrations of aquozinc(II). The line broadening was dependent upon the concentration of the metalloenzyme and independent of the frequency of the exciting radiation. When zinc ion was removed from the metalloenzyme by dialysis or when chloride was titrated from the metalloenzyme by cyanide, line broadening was decreased. Treatment of renal dipeptidase with saturating concentrations of the competitive inhibitor, guanosine triphosphate, in the presence of 0.5 M NaCl also produced a significant decrease in the 35Cl line width. The 35Cl line broadening produced by renal dipeptidase was shown to decrease with increasing pH through the range pH 5.8-10.8. This line-width variation with pH appeared to result from the titration of a site on the metalloprotein with an approximate pK of 7.4. Temperature studies of 35Cl line broadening by the metalloenzyme in the presence of chloride and cyanide inhibitors suggest that the fast exchange process pertains and that the dominant relaxation mechanism is quadrupolar in nature.     

Spectroscopic measurements of the electron and ion temperatures and effective ion charge in current sheets formed in two- and three-dimensional magnetic configurations

The spatial distributions of the electron temperature and density, the effective and average ion charges, and the thermal and directed ion velocities in current sheets formed in two-dimensional magnetic fields and three-dimensional magnetic configurations with an X line were studied using spectroscopic and interference holographic methods. The main attention was paid to studying the time evolution of the intensities of spectral lines of the working-gas (argon) and impurity ions under different conditions. Using these data, the electron temperature was calculated with the help of an original mathematical code based on a collisional-radiative plasma model incorporating the processes of ionization and excitation, as well as MHD plasma flows generated in the stage of the current-sheet formation. It is shown that the electron temperature depends on the longitudinal magnetic field, whereas the ion temperature is independent of it. The effective ion charge of the current-sheet plasma was determined for the first time.     

NMR studies on solution structure of single-stranded oligonucleotides causing line broadening.

Unusual line broadening of 1H-NMR lines attributable to the proton (8H) of guanine residues was observed for all tetradeoxyoligonucleotides tested here which have a specific base sequence of dGXXG (X = A or T). For the same samples, line broadening was also obtained in the 31P-NMR spectra. These broadened signals did not become sharp up to 60 degrees C. This unusual spectral phenomenon has been attributed by 2D-NMR and differential NOE to the compact solution structure of the oligonucleotides.