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<Emphasis Type="Italic">Arabidopsis rd29A::DREB1A</Emphasis> enhances freezing tolerance in transgenic potato 总被引:2,自引:0,他引:2
Behnam B Kikuchi A Celebi-Toprak F Kasuga M Yamaguchi-Shinozaki K Watanabe KN 《Plant cell reports》2007,26(8):1275-1282
The freezing tolerance of 38 independent transgenic potato lines derived from the cultivar Desiree was tested in vitro using
plantlets. The lines were transgenic for the DREB1A gene under control of the rd29A promoter, both of which were derived from Arabidopsis thaliana. The level of damage caused by freezing varied significantly among the transgenic clones and a non-transgenic control (cv.
Desiree). Phenotypic evaluation indicated that the variable responses to freezing were attributable to genotypic variation,
but freezing tolerance was not dependent on the number of insertions. Northern blot analysis using a DREB1A cDNA probe revealed high levels of DREB1A expression among the transgenic clones during the initial cold exposure at 4°C (after 2 h) and in the early stages of freezing
(−20°C, 1–10 min). Furthermore, a linear correlation was detected between the level of expression and the phenotypic response
for all lines except D138. Thus, in the case of potato, a significant increase in freezing tolerance was observed in vitro
on a small scale following the introduction of rd29A::DREB1A. Additional testing will show whether this strategy can be used for tolerance breeding in potato and to increase the freezing
tolerance of other agriculturally important crops.
Babak Behnam and Akira Kikuchi equally contributed for this work. 相似文献
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Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants
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Madoka Kudo Satoshi Kidokoro Takuya Yoshida Junya Mizoi Daisuke Todaka Alisdair R. Fernie Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《Plant biotechnology journal》2017,15(4):458-471
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HONG Bo TONG Zheng MA Nan LI Jianke KASUGA Mie YAMAGUCHI-SHINOZAKI Kazuko GAO Junping 《中国科学C辑(英文版)》2006,49(5):436-445
DNA cassette containing an AtDREB1A cDNA and a nos terminator, driven by a cauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into the ground cover chrysanthemum (Dendranthema grandiflorum) ‘Fall Color’ genome. Compared with wild type plants, severe growth retardation was observed in 35S:DREB1A plants, but not in rd29A:DREB1A plants. RT-PCR analysis revealed that, under stress conditions, the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants, but was over-expressed inductively in rd29A:DREB1A plants. The transgenic plants exhibited tolerance to drought and salt stress, and the tolerance was significantly stronger
in rd29A:DREB1A plants than in 35S:DREB1A plants. Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions. These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum, and improvement of the stress tolerance may be related
to enhancement of proline content and SOD activity. 相似文献
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Bo Hong Zheng Tong Nan Ma Jianke Li Mie Kasuga Kazuko Yamaguchi-Shinozaki Junping Gao 《中国科学:生命科学英文版》2006,49(5):436-445
DNA cassette containing an AtDREB1A cDNA and a nos terminator, driven by a cauliflower mosaic 35S promoter, or a stress-inducible rd29A promoter, was transformed into the ground cover chrysanthemum (Dendranthema grandiflorum) ‘Fall Color’ genome. Compared with wild type plants, severe growth retardation was observed in 35S:DREB1A plants, but not in rd29A:DREB1A plants. RT-PCR analysis revealed that, under stress conditions, the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants, but was over-expressed inductively in rd29A:DREB1A plants. The transgenic plants exhibited tolerance to drought and salt stress, and the tolerance was significantly stronger in rd29A:DREB1A plants than in 35S:DREB1A plants. Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions. These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum, and improvement of the stress tolerance may be related to enhancement of proline content and SOD activity. 相似文献
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通过花粉管通道技术,以该实验室自育陆地棉品系TH1和TH2为材料,将诸葛菜(Orychophragmus vidaceus)抗逆转录因子OvDREB2B基因构建到植物表达载体后,导入棉花基因组,经卡那霉素筛选和分子鉴定表明目的基因已整合到棉花基因组中并表达。将T1代转基因植株和受体对照在温室中栽培,待植株生长至四叶一心时,用不同渗透势的PEG-6000水溶液进行渗透胁迫处理,分析探讨转基因植株的抗旱效果及其抗旱机理。结果显示:当渗透势为0和0.5 MPa处理时,转基因植株和对照无明显差异;当渗透势为0.8 MPa和1.1 MPa处理时转基因植株较对照抗旱性明显提高。当渗透势为1.1 MPa处理96 h时,对照植株Fv/Fm降至0.2左右,而转基因植株仍正常生长,Fv/Fm值约为0.51,而且初始荧光(F0)值、净光合速率(Pn)、胞间CO2浓度(Ci)、蒸腾速率(Tr)等一系列参数转基因植株都明显优于对照,表明DREB2B基因能够提高棉花对水分胁迫的耐受性。 相似文献
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Simultaneous expression of regulatory genes associated with specific drought‐adaptive traits improves drought adaptation in peanut
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Vemanna S. Ramu Thavarekere N. Swetha Shekarappa H. Sheela Chandrashekar K. Babitha Sreevathsa Rohini Malireddy K. Reddy Narendra Tuteja Chandrashekar P. Reddy Trichi Ganesh Prasad Makarla Udayakumar 《Plant biotechnology journal》2016,14(3):1008-1020
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将克隆于羽衣甘蓝的胁迫应答基因BoRS1连入中间载体p35S-2300::gus::noster相应位点,成功地构建了含BoRS1基因的植物双元表达载体p35S-2300::BoRS1::noster,并通过农杆菌介导法对烟草进行了遗传转化。PCR检测结果表明目的基因BoRS1已成功地导入并整合到烟草基因组中。RT-PCR分析显示,在不同的转基因烟草植株中BoRS1表达量存在差异。转BoRS1烟草的耐干性和甘露醇胁迫研究表明,BoRS1基因的表达对提高植物抗干旱胁迫能力有一定的作用。 相似文献