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1.
Candida albicans is a polymorphic organism that grows under certain conditions as blastospores, hyphae or pseudohyphae. The potentials of FTIR spectroscopy for assessing structural differences in C. albicans blastospores and hyphae were investigated. The main observed differences were localised in the polysaccharide (950–1,185 cm−1), protein (1,480–1,720 cm−1), and the fatty acids (2,840–3,000 cm−1) regions. Quantitative evaluation of differences between hyphae and blastospores by curve-fitting of these regions indicate that these modifications could be due to both changes in structure and content of components of the cell wall such as β-glucans, mannoproteins, and lipids. Furthermore, glycogen consumption could be involved during hyphae elongation. Thus, FTIR spectroscopy can be an interesting tool to investigate differences in structure and in content between blastospores and hyphae. We also demonstrate through this study that differentiation of C. albicans clinical strains using hyphae is feasible, as this has been previously shown with blastospores. This preliminary work on identification of C. albicans using hyphae is a prelude to a larger clinical study for early typing within 7 h from a pure culture. 相似文献
2.
Marc Swidergall Norma V. Solis Nicolas Millet Manning Y. Huang Jianfeng Lin Quynh T. Phan Michael D. Lazarus Zeping Wang Michael R. Yeaman Aaron P. Mitchell Scott G. Filler 《PLoS pathogens》2021,17(1)
During oropharyngeal candidiasis (OPC), Candida albicans invades and damages oral epithelial cells, which respond by producing proinflammatory mediators that recruit phagocytes to foci of infection. The ephrin type-A receptor 2 (EphA2) detects β-glucan and plays a central role in stimulating epithelial cells to release proinflammatory mediators during OPC. The epidermal growth factor receptor (EGFR) also interacts with C. albicans and is known to be activated by the Als3 adhesin/invasin and the candidalysin pore-forming toxin. Here, we investigated the interactions among EphA2, EGFR, Als3 and candidalysin during OPC. We found that EGFR and EphA2 constitutively associate with each other as part of a heteromeric physical complex and are mutually dependent for C. albicans-induced activation. Als3-mediated endocytosis of a C. albicans hypha leads to the formation of an endocytic vacuole where candidalysin accumulates at high concentration. Thus, Als3 potentiates targeting of candidalysin, and both Als3 and candidalysin are required for C. albicans to cause maximal damage to oral epithelial cells, sustain activation of EphA2 and EGFR, and stimulate pro-inflammatory cytokine and chemokine secretion. In the mouse model of OPC, C. albicans-induced production of CXCL1/KC and CCL20 is dependent on the presence of candidalysin and EGFR, but independent of Als3. The production of IL-1α and IL-17A also requires candidalysin but is independent of Als3 and EGFR. The production of TNFα requires Als1, Als3, and candidalysin. Collectively, these results delineate the complex interplay among host cell receptors EphA2 and EGFR and C. albicans virulence factors Als1, Als3 and candidalysin during the induction of OPC and the resulting oral inflammatory response. 相似文献
3.
Yusuke Nakanishi Akira Hosono Yasuhiro Hiramatsu Teiji Kimura Ryo Nakamura Shuichi Kaminogawa 《Cytotechnology》2005,47(1-3):69-77
We demonstrate immunomodulatory effects, especially those involving murine intestinal IgA secretion, in Peyer's patch cells
following oral administration of Bifidobacterium immunomodulator (BIM) derived from sonicated B. pseudocatenulatum 7041. BALB/c mice were administered BIM orally for 7 consecutive days. The PP cells demonstrated upregulated secretion of
total IgA including BIM-specific IgA following BIM administration. In observing the response of PP cells co-cultured with
BIM, we found enhanced secretion of interferon-γ (IFN-γ) and interleukin (IL)-6 in the CD4+ T cells. In contrast, IL-12 secretion by Thy1.2− PP cells was enhanced, but secretion of IFN-γ, IL-5, and IL-6 was not significantly affected. Furthermore, the population
of CD4+ CD45RBhigh T cells in PP increased following oral administration of BIM. These data suggest that CD4+ T cells were affected by BIM administration. Overall, the results show that oral administration of BIM induced CD4+ PP cells to change their expression of cell surface antigen and cytokine production. 相似文献
4.
Carolina Rodrigues Costa Rosália Santos Amorim Jesuíno Janine de Aquino Lemos Orionalda de Fátima Lisboa Fernandes Lúcia Kioko Hasimoto e Souza Xisto Sena Passos Maria do Rosário Rodrigues Silva 《Mycopathologia》2010,169(2):91-98
Some antifungal agents have shown to exert effects on expression of virulent factors of Candida as the production of secretory aspartyl proteinase (Sap). In this study, we sought to determine and to compare the influence
of fluconazole and voriconazole in proteinase activity of this microorganism. Thirty-one isolates obtained from oral mucosa
of human immunodeficiency virus positive (HIV+) patients were used in this study. The minimal inhibitory concentrations (MIC) of fluconazole and voriconazole were determined
using the broth microdilution method with RPMI 1640 medium and with yeast carbon base–bovine serum albumin (YCB–BSA) medium.
The Sap activity following by digestion of BSA as substrate was determined for four Candida albicans strains arbitrarily chosen according to susceptibility (susceptible or resistant) to fluconazole or voriconazole. Besides,
the SAP1 to SAP7 genes were screened by PCR for the same isolates that were determined by the Sap activity. In vitro susceptibility testing
using the two media presented similar MIC values. Increased Sap activity was observed in resistant isolates on presence of
drugs, but the Sap activity by susceptible isolates to azoles showed different behavior on the presence of drug. We detected
the presence of SAP1 to SAP7 genes from all susceptible or resistant C. albicans isolates. The present study provides important data about the proteinase activity and the presence of genes of SAP family in fluconazole and voriconazole susceptible or resistant C. albicans isolates. 相似文献
5.
An indirect immunofluorescence assay was performed to detect antibodies toCandida albicans blastospores and germ tubes. Serum specimens were obtained from 82 patients with neoplastic diseases in the orofacial region
and thrush of the oral mucosa.C. albicans was identified in the oral cavity of 63 patients investigated but serum anti-Candida antibodies were detected in only 23 of them. Serological examination showed that titers of antibodies toC. albicans blastospores ranged from 1∶20 to 1∶1280. High titers from 1∶640 to 1∶1280 were detected in patients without antibiotic, cytostatic,
or radiotherapeutic treatment. The titers of antibodies toC. albicans germ tubes ranged from 1∶20 to 1∶640. Our results indicate that titers of antibodies to theC. albicans germ tubes were lower and were detected in a smaller number of patients. 相似文献
6.
Lipopolysaccharide of Escherichia coli, polyamines, and acetic acid stimulate cell proliferation in intestinal epithelial cells 总被引:1,自引:0,他引:1
Jaime Olaya Vadim Neopikhanov Andrés Uribe 《In vitro cellular & developmental biology. Animal》1999,35(1):43-48
Summary Our aim was to examine whether lipopolysaccharide of Escherichia coli, polyamines of dietetic and/or bacterial origin, and products of the bacterial metabolism influence cell proliferation in
epithelial cells from the colon and small intestine. Lipopolysaccharide of Escherichia coli 0111:B4 was incubated with cultures from human colonic mucosa. The mitoses were arrested with Vincristine and the total number
of metaphases per crypt was counted. In addition, lipopolysaccharide was incubated with a human colonic epithelial cell line
from adenocarcinoma (LS-123 cells) and with a nontransformed small intestinal cell line from germ-free rats (IEC-6 cells)
for 24 h. In the last 4 h, the cells were labeled with tritiated thymidine. The cells were incubated with putrescine, cadaverine,
and spermidine at 10−11–10−3
M and with acetic acid (10−5–10−1
M), acetaldehyde (10−10–10−4
M) and ammonium chloride (1–20 mM). Lipopolysaccharide of Escherichia coli increased the number of arrested metaphases in human colonic crypts and DNA synthesis in L-123 and IEC-6 cells (P<0.001). All polyamines increased DNA synthesis in the colonic and small intestinal cell lines, the effects being more marked
for putrescine (P<0.001). The higher concentrations of acetic acid increased DNA synthesis in both epithelial cell lines (P<0.001). Acetaldehyde slightly decreased DNA synthesis in LS-123 cells at cytotoxic concentrations. Ammonium chloride did
not significantly affect DNA synthesis. The final concentration of nonionized ammonia was less than 3%. It is concluded that
lipopolysaccharides of Escherichia coli and intraluminal factors derived from microorganisms increase cell proliferation in human colonic crypts and intestinal epithelial
cell lines. 相似文献
7.
The conversion of a cellulose-producing cell (Cel
+) fromGluconacetobacter hansenii PJK (KCTC 10505 BP) to a non-cellulose-producing cell (Cel
−) was investigated by measuring the colony forming unit (CFU). This was achieved in a shaking flask with three slanted baffles,
which exerted a strong shear stress. The addition of organic acid, such as glutamic acid and acetic acid, induced the conversion
of microbial cells from a wild type toCel
− mutants in a flask culture. The supplementation of 1% ethanol to the medium containing an organic acid depressed the conversion
of the microbial cells toCel
− mutants in a conventional flask without slanted baffles. The addition of ethanol to the medium containing an organic acid;
however, accelerated the conversion of microbial cells in the flask with slanted baffles. TheCel
+ cells from the agitated culture were not easily converted intoCel
−, mutants on the additions of organic acid and ethanol to a flask without slanted baffles, but some portion of theCel
+ cells were converted toCel
− mutants in a flask with slanted baffles. The conversion ratio ofCel
+ cells toCel
− mutants was strongly related to the production of bacterial cellulose independently from the cell growth. 相似文献
8.
Orlov SN Dulin NO Gagnon F Gekle M Douglas JG Schwartz JH Hamet P 《The Journal of membrane biology》1999,172(3):225-234
We demonstrated recently that in renal epithelial cells from collecting ducts of Madin-Darby canine kidneys (MDCK), Na+,K+,Cl− cotransport is inhibited up to 50% by ATP via its interaction with P2Y purinoceptors (Biochim. Biophys. Acta 1998. 1369:233–239). In the present study we examined which type of renal epithelial cells possesses the highest sensitivity of Na+,K+,Cl− cotransport to purinergic regulation. We did not observe any effect of ATP on Na+,K+,Cl− cotransport in renal epithelial cells from proximal and distal tubules, whereas in renal epithelial cells from rabbit and
rat collecting ducts ATP decreased the carrier's activity by ∼30%. ATP did not affect Na+,K+,Cl− cotransport in C7 subtype MDCK cells possessing the properties of principal cells but led to ∼85% inhibition of this carrier
in C11-MDCK cells in which intercalated cells are highly abundant. Both C7- and C11-MDCK exhibited ATP-induced IP3 and cAMP production and transient elevation of [Ca2+]
i
. In contrast to the above-listed signaling systems, ATP-induced phosphorylation of ERK and JNK MAP kinases was observed in
C11-MDCK only. Thus, our results reveal that regulation of renal Na+,K+,Cl− cotransport by P2Y receptors is limited to intercalated cells from collecting ducts and indicate the involvement of the MAP kinase cascade in
purinergic control of this ion carrier's activity.
Received: 10 June 1999/Revised: 23 August 1999 相似文献
9.
Susana Johann Daniele L. Silva Cleide V. B. Martins Carlos L. Zani Moacir G. Pizzolatti Maria A. Resende 《World journal of microbiology & biotechnology》2008,24(11):2459-2464
Plants are known to produce a plethora of secondary metabolites which are recognized as a useful source of new drugs or drug
leads. Extracts and fractions of Schinus terebinthifolius Raddi (Anacardiaceae), Piper regnellii C.D.C. (Piperaceae), Rumex acetosa L. (Polygonaceae), and Punica granatum L. (Punicaceae) were assessed for their antifungal activity against eight clinical isolates of C. albicans. They were also evaluated for their effect on the adhesion of these C. albicans isolates to buccal epithelial cells (BECs). The ethyl acetate fraction from the leaves of S. terebinthifolius showed promising activity, inhibiting the growth of three C. albicans isolates at 7.8 μg ml−1 and significantly inhibiting their adhesion to BEC at 15 μg ml−1
. In addition, this fraction did not show cytotoxic activity against murine macrophages. The results show the potential of
the plant extracts studied as a source of new antifungal compounds. Further studies are necessary for isolation and characterization
of the active compounds of these plants. 相似文献
10.
We investigated the toxicity of synthetic antimicrobial decapeptide KSL-W on normal human gingival epithelial cell cultures, its effect on Candida albicans adhesion and growth, and the activation of epithelial cell innate immunity. Our results indicate that KSL-W had no toxic effect on cell adhesion or growth, suggesting its safe use with human cells. Pre-treating C. albicans with KSL-W attenuated the yeast's virulence as demonstrated by its reduced adhesion and growth on engineered human oral mucosa epithelium and the subsequent decreased expression of some innate defense molecules by targeted epithelial cells. Indeed, the expression of Toll-like receptors and human β-defensins was reduced in tissues infected with KSL-W-treated Candida. Proinflammtory cytokine secretion (IL-1β and IL-6) by the epithelial cells was also regulated by KSL-W in a manner similar to that of antifungal molecule amphotericin B. These findings therefore show that KSL-W is safe for use with human cells and is able to attenuate Candida virulence by modulating its effects on host innate immunity. This study proposes the potential application of KSL-W peptide as an alternative antifungal agent. 相似文献
11.
Takao Nohmi Shigeru Abe Kazuyoshi Dobashi Shigeru Tansho Hideyo Yamaguchi 《Microbiology and immunology》1995,39(6):405-409
Sex steroid hormones were examined for their effect on mycelial growth of Candida albicans, and the inhibitory activity of casein-induced murine peritoneal neutrophils against mycelial growth of C. albicans was examined in vitro using a crystal violet staining method or a [3H]glucose incorporation method. Four steroid hormones, danazol, estradiol, estriol and testosterone had no effect on mycelial growth of C. albicans, but progesterone appeared to convert the growth form of C. albicans from hyphal to yeast. Danazol (10–6 m ) and progesterone (10–5 m ) suppressed anti-Candida activity of neutrophils of non-treated mice, while testosterone, estradiol, and estriol did not. The anti-Candida activity of neutrophils of estradiol-pretreated mice was clearly suppressed by progesterone even at 10–6 m which corresponded to its plasma concentration in pregnant women in the third trimester. The physiological significance of this suppressive effect of progesterone was discussed in relation to the vulnerability of pregnant women to vaginal candidiasis. 相似文献
12.
Anti-Candida activity of murine neutrophils and its regulation by immunomodulators were studied in vitro. Murine neutrophils which were prepared from peritoneal-exudated cells inhibited the growth of Candida albicans at an effector: target (E/T) ratio of 30/1 or above. This anti-Candida activity of neutrophils was augmented by lipopolysaccharide from Escherichia coli, murine tumor necrosis factor (TNF), murine interferon-γ (IFN-γ) and murine granulocyte macrophage colony-stimulating factor (GM-CSF) but not by granulocyte colony-stimulating factor (G-CSF) added to the incubation medium. Greater extent of augmentation was obtained when TNF plus GM-CSF or INF-γ plus GM-CSF were used in combination. These results indicate that anti-Candida activity of murine neutrophils is regulated similarly to that of the human neutrophils reported previously. Therefore murine peritoneal neutrophils can be used as a favorable substitute for human neutrophils in studies on protective machinery against C. albicans infection. 相似文献
13.
Kerstin Trautwein-Weidner André Gladiator Florian R. Kirchner Simone Becattini Thomas Rülicke Federica Sallusto Salomé LeibundGut-Landmann 《PLoS pathogens》2015,11(10)
Candida spp. can cause severe and chronic mucocutaneous and systemic infections in immunocompromised individuals. Protection from mucocutaneous candidiasis depends on T helper cells, in particular those secreting IL-17. The events regulating T cell activation and differentiation toward effector fates in response to fungal invasion in different tissues are poorly understood. Here we generated a Candida-specific TCR transgenic mouse reactive to a novel endogenous antigen that is conserved in multiple distant species of Candida, including the clinically highly relevant C. albicans and C. glabrata. Using TCR transgenic T cells in combination with an experimental model of oropharyngeal candidiasis (OPC) we investigated antigen presentation and Th17 priming by different subsets of dendritic cells (DCs) present in the infected oral mucosa. Candida-derived endogenous antigen accesses the draining lymph nodes and is directly presented by migratory DCs. Tissue-resident Flt3L-dependent DCs and CCR2-dependent monocyte-derived DCs collaborate in antigen presentation and T cell priming during OPC. In contrast, Langerhans cells, which are also present in the oral mucosa and have been shown to prime Th17 cells in the skin, are not required for induction of the Candida-specific T cell response upon oral challenge. This highlights the functional compartmentalization of specific DC subsets in different tissues. These data provide important new insights to our understanding of tissue-specific antifungal immunity. 相似文献
14.
The identification and separation of small intestinal epithelial stem cells are still on the preliminary stage. In this study,
we planned to utilize immunohistochemistry, fluorescence-activated cell sorting (FACS) and RT-PCR to investigate the possibility
of CD133 and CD44 as markers of human small intestinal epithelial stem cells. The expressions of CD133, CD44 and Lgr5 were
studied by immunohistochemistry. Four subgroups of CD133+CD44+, CD133+CD44−, CD133−CD44+, CD133−CD44− were sorted out through FACS and the expression level of Lgr5 gene was measured by RT-PCR and polyacrylamide gel electropheresis (PAGE) with sliver stained. Ten cases of samples were
available for analyzing. By immunohistochemical staining, few cells with positive expressions of CD133, CD44 and Lgr5 were
distributed in the bottom of crypts with the expression locations somewhat overlapped. The average percentage of CD133+CD44+ cells was 0.0580 ± 0.0403%, while the corresponding contents of CD133+CD44− cells, CD133−CD44+ cells and CD133−CD44− cells were 0.4000 ± 0.1225%, 0.7000 ± 0.2646% and 76.5600 ± 3.5529% respectively. Ten times of positive expressions of Lgr5 were detected in the CD133+CD44+ groups, while 9/10, 8/10 and 4/10 times for CD133+CD44−, CD133−CD44+ and CD133−CD44− subgroups respectively. With the help of Quantityone 4.62 software, the densities of corresponding place to Lgr5 and reference gene were obtained. The density ratios of corresponding place to Lgr5 to reference gene were significant difference between subgroups (P < 0.001). By means of LSD method, the density ratios in CD133+CD44+ subgroups had statistical differences from the other subgroups (P < 0.05). We concluded CD133+CD44+ cells may be human small intestinal epithelial stem cells, which need further researches to confirm. 相似文献
15.
This study compared the effects of the human 70-kDa stress protein (Hsp70) peptide, TKDNNLLGRFELSG (TKD), proinflammatory
cytokines, or a combination of both on the repertoire of receptors expressed by human natural killer (NK) cells and their
capacity to kill human CX colon carcinoma cells, K562 erythroleukemic cells, and leukemic blasts from two patients with acute
myelogenous leukemia. Low-dose interleukin (IL) 2/IL-15 and TKD increase the expression density of activatory (NKG2D, NKp30,
NKp44, NKp46, CD94/NKG2C) and inhibitory (CD94/NKG2A) receptors on NK cells. Concomitantly, IL-2/TKD treatment enhances the
cytotoxicity of NK cells (as reflected by their secretion of granzyme B) against Hsp70 membrane-positive and human leukocyte
antigen (HLA)-E membrane-negative (Hsp70+/HLA-E−) CX+ and K562 cells. However, it had no effect on the responsiveness to Hsp70−/HLA-E− CX− cells over that induced by IL-2 alone. The cytotoxicity of IL-2/TKD-activated, purified NK cells and peripheral blood mononuclear
cells against Hsp70+/HLA-E+ leukemic blasts was weaker than that against Hsp70+/HLA-E− K562 cells. Hsp70-blocking and HLA-E transfection experiments confirmed membrane-bound Hsp70 as being a recognition/activatory
ligand for NK cells, as cytotoxicity was reduced by the presence of the anti-Hsp70 monoclonal antibody cmHsp70.2 and by inhibiting
Hsp70 synthesis using short interference ribonucleic acid. HLA-E was confirmed as an inhibitory ligand, as the extent of NK
cell-mediated lysis of K562 cell populations that had been transfected with HLA-ER or HLA-EG alleles was dependent on the proportion of HLA-E-expressing cells. These findings indicate that Hsp70 (as an activatory molecule)
and HLA-E (as an inhibitory ligand) expression influence the susceptibility of leukemic cells to the cytolytic activities
of cytokine/TKD-activated NK cells. 相似文献
16.
Cell pH regulation was investigated in the T84 cell line derived from epithelial colon cancer. Cell pH was measured by ratiometric
fluorescence microscopy using the fluorescent probe BCECF. Basal pH was 7.17 ± 0.023 (n= 48) in HEPES Ringer. After acidification by an ammonium pulse, cell pH recovered toward normal at a rate of 0.13 ± 0.011
pH units/min in the presence of Na+, but in the absence of this ion or after treatment with 0.1 mm hexamethylene amiloride (HMA) no significant recovery was observed, indicating absence of Na+ independent H+ transport mechanisms in HEPES Ringer. In CO2/HCO−
3 Ringer, basal cell pH was 7.21 ± 0.020 (n= 35). Changing to HEPES Ringer, a marked alkalinization was observed due to loss of CO2, followed by return to the initial pH at a rate of −0.14 ± 0.012 (n= 8) pH/min; this return was retarded or abolished in the absence of Cl− or after addition of 0.2 mm DIDS, suggesting extrusion of bicarbonate by Cl−/HCO−
3 exchange. This exchange was not Na+ dependent. When Na+ was added to cells incubated in 0 Na+ Ringer while blocking Na+/H+ exchange by HMA, cell alkalinization by 0.19 ± 0.04 (n= 11) pH units was observed, suggesting the presence of Na+/HCO−
3 cotransport carrying HCO−
3 into these cells, which was abolished by DIDS. These experiments, thus, show that Na+/H+ and Cl−/HCO−
3 exchange and Na+/HCO−
3 cotransport participate in cell pH regulation in T84 cells.
Received: 3 April 2000/Revised: 22 June 2000 相似文献
17.
Anti-Candida antibodies were determined in a group of preterm neonates from a neonatal intensive care unit with serious diseases including
candidemia. Antibodies toC. albicans blastospores,i.e. antibodies toC. albicans surface mannan and toC. albicans germ tubes were detected. Higher titers of antibodies to blastospores (1:320) occurred in all patients examined while antibodies
toC. albicans germ tubes (with the highest titer of 1:160) were present in 32 out of 66 neonates examined. The highest titers of both anti-C. albicans blastospore antibodies and anti-C. albicans germ tube antibodies were detected in neonates with candidemia and disorders of saccharide metabolism. 相似文献
18.
K. V. Shmagel L. B. Korolevskaya E. V. Saidakova N. G. Shmagel V. A. Chereshnev L. Margolis D. Anthony M. Lederman 《Doklady biological sciences》2017,477(1):244-247
The level of proinflammatory markers was assessed in HIV-infected patients that were coinfected with hepatitis C virus (HCV) and had failed to restore the CD4+ T cell counts (immunological nonresponders, INR) during the antiretroviral therapy (ART). Among four patient groups (HIV+HCV– and HIV+HCV+ subjects with the concordant response to ART; HIV+HCV– and HIV+HCV+ subjects that were INR), the greatest systemic inflammation was in the latter group. The maximum difference was between the subjects HIV+HCV–INR and HIV+HCV+ INR: the blood of coinfected patients contained significantly higher concentrations of the IP-10, sCD163, sTNF-RI, and sTNF-RII and of bacterial lipopolysaccharide. Systemic inflammation in HIV/HCV coinfected patients with the discordant response to ART is probably caused by a breach of hepatic barrier for the intestine products. 相似文献
19.
K. Kunzelmann D. C. Lei K. Eng L. C. Escobar T. Koslowsky D. C. Gruenert 《In vitro cellular & developmental biology. Animal》1995,31(8):617-624
Summary Analysis of vectorial ion transport and protein trafficking in transformed cystic fibrosis (CF) epithelial cells has been
limited because the cells tend to lose their tight junctions with multiple subcultures. To elucidate ion transport and protein
trafficking in CF epithelial cells, a polar cell line with apical and basolateral compartments will facilitate analysis of
the efficacy of different gene therapy strategies in a “tight epithelium”in vitro. This study investigates the genotypic and phenotypic properties of a CF nasal polyp epithelial, ΔF508 homozygote, cell line
that has tight junctions pre-crisis. The cells (ΣCFNPE14o-) were transformed with an origin-of-replication defective SV40
plasmid. They develop transepithelial resistance in Ussing chambers and are defective in cAMP-dependent Cl− transport as measured by efflux of radioactive Cl−, short circuit current (Isc), or whole-cell patch clamp. Stimulation of the cells by bradykinin, histamine, or ATP seems to activate both K+- and Ca+2-dependent Cl− transport. Measurement of36Cl− efflux following stimulation with A23187 and ionomycin indicate a Ca+2-dependent Cl− transport. Volume regulatory capacity of the cells is indicated by cell swelling conductance. Expression of the CF transmembrane
conductance regulator mRNA was indicated by RT-PCR amplification. When cells are grown at 26° C for 48 h there is no indication
of cAMP-dependent Cl− as has been previously indicated in heterologous expression systems. Antibodies specific for secretory cell antigens indicate
the presence of antigens found in goblet, serous, and mucous cells; in goblet and serous cells; or in goblet and mucous cells;
but not antigens found exclusively in mucous or serous cells. Gene complementation studies with an episomal vector containing
wild-type CF transmembrane conductance regulator cDNA showed correction of the cAMP-dependent Cl− transport defect. This cell line contributes unique phenotypic features to the store of transformed CF epithelial cells already
available. 相似文献
20.
Lucie Colineau Angeline Rouers Takuya Yamamoto Yin Xu Alejandra Urrutia Hang-Phuong Pham Sylvain Cardinaud Assia Samri Karim Dorgham Pierre-Grégoire Coulon Rémi Cheynier Anne Hosmalin Eric Oksenhendler Adrien Six Anthony D. Kelleher John Zaunders Richard A. Koup Brigitte Autran Arnaud Moris Stéphanie Graff-Dubois 《PloS one》2015,10(10)