提高《生物工程设备》课程教学质量的探索与实践

《生物工程设备》是生物工程专业本科生的学科专业课程。针对《生物工程设备》课程的教学现状,本着调整课程教学体系、优化教学内容、革新教学方法和手段、重视实践教学基地建设和师资队伍建设、注重培养学生创新能力等形式和原则, 对课程进行了教学改革和实践。     

提高《生物工程设备》课程教学质量的探索与实践

《生物工程设备》是生物工程专业本科生的学科专业课程。针对《生物工程设备》课程的教学现状,本着调整课程教学体系、优化教学内容、革新教学方法和手段、重视实践教学基地建设和师资队伍建设、注重培养学生创新能力等形式和原则,对课程进行了教学改革和实践。     

“生物工程设备”国家一流本科课程建设与实践

生物技术产业是我国战略新兴产业,近年来发展极为迅速,对实践创新型人才需求极大。作为生物工程专业核心课程,“生物工程设备”在生物技术产业实践创新型人才培养中具有重要意义。本课程针对当前课程教学中的设备可及性低、工程实训机会少和学生学习缺乏主动性等痛点,以设备资源和素材库建设、虚实结合工程实训、学生学习积极性调动为抓手,构建“育德、育智、育能(实践创新能力)、对接社会发展新需求、融入学科前沿新进展、引入科研实践新成果(三育三新)”教学内容,创新教学方式和优化考核评价,通过课内外联动-校内外联动、引入前沿进展及科研成果、多样化资源集成、线上线下结合等手段,提高学生的主动性和创新能力,以达到“三会一能”。通过教学探索和实践,提高了课程教学的有效性,学生实践创新能力显著提升。     

热烈庆祝镇江东方生物工程设备技术公司成立10周年！

东方生物工程公司经过十年的不断发展,已经取得了相当的成就,在这里我们感谢各位用户对我们的信任和支持!祝愿我们的朋友们鹏程万里,与我们携手走向更美好的明天。     

生物工程专业酶工程网络课程运行模式的实践与探索

酶工程网络课程建设是生物工程专业教学模式改革的重要部分,通过网络平台可将学生与授课教师有机结合,同时能更好地整合教学资源,提高学习效果,优化学习结构,为学生多媒体教学后的复习与知识拓展、疑难问题的及时解决创造了良好的条件。因此,为了进一步提高网络课程的建设质量,需优化课程设计体系,突出网络学习优点,并紧密结合学科发展前沿和本院教师科研方向,以促进网络课程的应用及实践。     

生物工程专业课程群的改革探索

在对本校生物工程专业人才培养过程中发现的问题入手,对专业课程群建设的现状进行分析,对于优化课程体系以及相关教学内容的有效衔接、强化学生工程实践能力培养等方面提出了相应的改革思路,以期提高本专业的办学水平。     

生物工程专业微生物学教学改革与实践

针对生物工程专业微生物学理论教学与实验教学如何围绕学生工程能力和创新能力的培养组织教学进行探讨,从教材选定,教学内容重组与更新,培养学生的科学精神和综合思考能力,注重理论联系实际以及教学方法、教学手段、实验考核制度的改革等进行了一些有益的尝试,取得了较为满意的效果。     

生物工程专业发酵课程群建设探索

针对生物工程专业的培养方案和就业方向,提出了以课程群建设推动专业发展的教学改革思路。以\"生物工艺学\"和\"生物反应工程\"为主干课,还包括\"生物工程设备\"、\"代谢控制与发酵\"、\"生物过程检测与控制\"等相关课程,同时结合\"发酵工艺实验\"、\"生产实习\"和\"毕业设计\"等实践教学环节,构建了发酵课程群。介绍了发酵课程群对教学内容的整合,分析了课堂教学与实践环节有机结合的教学模式。实践证实,发酵课程群的建设对学生的就业以及后续工作能力的提升起到了显著的促进作用。     

生物工程专业实验教学改革与实践

从生物工程专业实验的课程设置、实验内容、教学方式、考核方式以及为学生提供实验平台等方面进行改革及实践,提升了实验教学质量,提高了学生的整体素质,培养了学生的创新精神,为学生进一步深造或就业奠定了坚实基础。     

SPR@CEW教学模式在本科生物工程专业英语教学中的探索和实践

大学本科专业英语的教学目的是提升学生的专业英语阅读理解、语言表达和写作能力,拓展学生的专业视野。同时,专业英语学习对于提升学生毕业论文的写作能力、研究生入学英语考试成绩也具有非常重要的作用。面对专业英语的重要性,授课教师多年来在专业英语教学中一直积极探索并进行教学改革。基于本科学情,在教学中把科学引文索引(science citation index,SCI)英语科技论文阅读、大学实验英语小论文写作与专业英语的教和学相结合,建立SPR@CEW(SCI paper reading and college experiment writing)教学模式,促进了课堂教学与科研进展、理论学习与实际训练的有机融合。教学实践发现,SPR@CEW教学模式有效提升了专业英语的课程质量和吸引力,并取得了良好的教学效果。     

发展我国大规模细胞培养生物反应器装备制造业

生物反应器在生物工程装备制造业的产业化方面具有极其重要的意义。在分析了生物反应器产业的产品特点后,对我国生物反应器产业的现状和今后发展作了讨论。对以代谢流分析为核心的生物反应器、带pH测量与补料控制的摇床、动物细胞大规模培养生物反应器研制、生物反应器中试系统设计、大型生物反应器设计与制造技术研究等几个重要开发内容进行深入讨论,最后对细胞过程的系统生物学研究与生物反应器作了展望。     

Bioprocess intensification: a potential aqueous two-phase process for the primary recovery of B-phycoerythrin from Porphyridium cruentum

A process for the primary recovery of B-phycoerythrin from Porphyridium cruentum exploiting aqueous two-phase systems (ATPS) was developed in order to reduce the number of unit operations and benefit from an increased yield of the protein product. The evaluation of system parameters such as poly(ethylene glycol) (PEG) molecular mass, concentration of PEG as well as salt, system pH and volume ratio was carried out to determine under which conditions the B-phycoerythrin and contaminants concentrate to opposite phases. PEG 1450-phosphate ATPS proved to be suitable for the recovery of B-phycoerythrin because the target protein concentrated to the top phase whilst the protein contaminants and cell debris concentrated in the bottom phase. An extraction ATPS stage comprising volume ratio (Vr) equal to 1.0, PEG 1450 24.9% (w/w), phosphate 12.6% (w/w) and system pH of 8.0 allowed B-phycoerythrin recovery with a purity of 2.9 (estimated as the relation of the 545-280 nm absorbances). The use of ATPS resulted in a primary recovery process that produced a protein purity of 2.9 +/- 0.2 and an overall product yield of 77.0% (w/w). The results reported demonstrated the practical implementation of ATPS for the design of a primary recovery process as a first step for the commercial purification of B-phycoerythrin produced by P. cruentum.     

Addressing a whole bioprocess in real-time using an optical biosensor-formation,recovery and purification of antibody fragments from a recombinant<Emphasis Type="Italic"> E. coli</Emphasis> host

The use of biosensor technology is described to address in real-time the production and subsequent purification of a bioactive recombinant protein product. The product, D1.3 Fv antibody fragment, was expressed in Escherichia coli and purified via two process routes, one for extracellular and one for intracellular product material. The cells were harvested by centrifugation in a solid bowl CARR Powerfuge and stored at –70°C. Clarification of the supernatant was performed by depth filtration, followed by affinity chromatography for final purification of the extracellular product. To purify the intracellular product the harvested cells were resuspended and homogenised. Removal of debris in the CARR Powerfuge was followed by depth filtration and affinity chromatography. In this work we have shown the rapid determination of bioactive product levels, and the impact this has on improved accountability and confidence is demonstrated in process mass balances on the product using the data acquired during process operation.     

Asymmetric Biosynthesis of Key Aromatic Intermediates in the Synthesis of an Endothelin Receptor Antagonist

The feasibility of five potential biocatalytic routes were investigated for the chiral synthesis of key intermediates of an experimental endothelin receptor antagonist. Two asymmetric bioreductions of a ketoester and a chlorinated ketone to their corresponding chiral alcohol yielded very encouraging leads. Pichia delftensis (strain MY 1569) and Rhodotorula piliminae (ATCC 32762) were found to respectively bioreduce the esterified ketone and chlorinated substrate to their corresponding (S) alcohol with enantiomeric excesses > 98% and > 99% respectively. When scaled up in laboratory bioreactors (23-liter scale), both processes produced the desired (S) alcohol intermediate with elevated yield, about 88% and 97% for the ketoester and chloroketone respectively. Investigative chemical syntheses employing the (S) ester alcohol showed that unfavorable racemization occurred during the subsequent synthetic steps. However, the use of the (S) chloroalcohol as chiral synthon for the production of the endothelium receptor antagonist was successfully demonstrated at a preparative scale.