INVESTIGATIONS REGARDING THE NATURE OF THE INTERACTION BETWEEN IRON AND MOLYBDENUM OR VANADIUM IN NUTRIENT SOLUTIONS WITH AND WITHOUT A GROWING PLANT

Iron offset the toxicity of molybdenum or vanadium in nutrient solutions more effectively when it was supplied at the same time as the molybdenum or vanadium than when it was given separately in alternate 3-day periods.
Allowing nutrient solutions of pH 4.6 containing high concentrations of iron, with or without vanadium, to stand for 4 days before use did not delay the restoration of colour to chlorotic plants, but even z days' standing reduced the iron content of their roots and the vanadium content of both shoot and root. The presence of vanadium had little effect on iron uptake.
In parallel experiments with molybdenum, standing the solutions for 7–9 days before use delayed colour recovery, but shorter periods had no effect. Standing for z days or longer greatly reduced the iron content of the root, but the molybdenum content was unaffected or increased. High molybdenum greatly increased the iron in the root, but had little effect on that in the shoot.
Precipitation of iron in the nutrient solution was delayed by high concentrations of either ammonium or sodium molybdate if the initial pH was 4.6, but not if it was 6.6. Vanadium had no influence on the precipitation of iron at pH 4.6.
At least part of the compensating action of iron on molybdenum or vanadium toxicity would appear to take place outside the plant.     

SOME OBSERVATION ON AIR-ION-ENHANCED IRON CHLOROSIS IN BARLEY (HORDEUM VULGARIS) SEEDLINGS

The air ion effects on "active" and "residual" iron distributionin barley seedlings were studied in the course of the developmentof ironchlorosis in an iron-free culture medium. "Active" or"acid soluble" iron plays an important role for chlorophyllbiosynthesis and is extractable with 1.0 N HCl from dried tissues,and "residual" or "acid insoluble" iron does not participatein this chlorophyll formation process and is not extracted with1.0 N HCl. Ions of either charge induced a significant decrease in activeiron content which was associated with a decrease in chlorophyllcontent. Concomitantly, there occurred an increase in both theresidual iron and the cytochrome c fractions. The increase inresidual iron content may involve not only cytochrome c butalso other cytochromes and ironcontaining enzymes as well. Theauthors have proposed a hypothesis that the site of air ionaction in the experiments reported may be the regulatory systemscontrolling iron metabolism in the seed and young seedling.Through this action air ions apparently divert more endogenousfree-state iron to residual iron (consisting of cytochromesand Fe-containing enzymes) than to active iron. Tracer experiments showed that air ions enhanced the uptakeof exogenous iron by early germinating barley seeds. The increasedincorporation of iron was not influenced by light. (Received December 10, 1964; )     

Pelagic growth and colony division of Gloeotrichia echinulata in Lake Erken

Gloeotrichia echinulata colony development was monitored inLake Erken, Sweden and studied in enclosure experiments. Significantcolonial division did not occur in mesh bags, although the abundanceof the pelagic population in the lake increased during the experimentalperiods. On the basis of these findings, it is suggested thatcirculation of G. echinulata to deeper nutrient rich water supportspelagic growth. In support of this, a large part of the buoyantG. echinulata colonies in Lake Erken was found at several metersdepth. In an experiment with nutrient additions, the only treatmentthat favoured G. echinulata development was additions of phosphate,nitrate and iron. Trace element additions had a negative effecton the development of G. echinulata. On the basis of these findings,the nutritional requirements of G. echinulata are discussed.     

Breaking of Seed Dormancy by Nitrate as a Gap Detection Mechanism

Germination of Planlago lanceolata seeds buried in a chalk grasslandwas higher in bare soil than in vegetated soil, and measurementof soil nitrate concentrations showed that they were high enoughto account for this stimulation. When seeds of P. lanceolatawere sown in pots of soil with or without plants, and wateredwith nutrient solution containing either no nitrate, or 14 mMnitrate (sufficiently high that not all nitrate was absorbedby the plants), the presence of plants inhibited germinationonly when the nutrient solution contained no nitrate. It wasconcluded that breaking of seed dormancy by nitrate can functionas a gap detection mechanism if nitrate concentrations in baresoil are high enough to break seed dormancy, but are too lowto break dormancy when vegetation is present Plantago lanceolata, seed, dormancy, germination, nitrate, gap detection     

Influence of leaf age, light, dark, and iron deficiency on polyribosome levels in maize leaves

The relations between leaf age and polyribosome levels werestudied with dark-and light-grown maize (Zea mays L.) seedlings.In general, polyribosome levels decline with the period of growthin darkness. Light induces an increase in the polyribosome levelin dark-grown seedlings. The response can be detected after30 min exposure to light. Seven or eight-day-old dark-growncorn seedlings, used in the present study, have high levelsof polyribosomes when greened in the light. This is indicativeof healthy seedlings, competent in protein synthesis. The polyribosomelevels in iron deficient maize plants were significantly differentfrom plants grown under complete nutrient solution, while thereis no significant difference among plants suffering differentdegrees of iron deficiency. (Received November 18, 1977; )     

Active Iron in Plant Leaves

Tomato plants were grown with limited or luxury water supplyand given nitrogen either as the ammonium ion or the nitrateion. The ‘active’ iron fractions of the leaves asextracted by etherized tenth molar hydrochloric acid showedno relationship with the total iron but a very significant linearrelationship was found between the active iron fraction andthe ratio of total phosphorus to total iron. Recalculation of results presented by other investigators formaize plants grown in soil with various levels of moisture,phosphorus and iron also showed a highly significant linearrelationship between the active iron fraction and the ratioof total phosphorus to total iron. Lycopersicon esculentum L., tomato, ‘active iron’, phosphorus, Zea mays     

Translocation of iron to the N2-fixing stem nodules of Sesbania rostrata (Brem)

Labelled iron (applied as ⁵⁵FeCl₃) moves rapidly from the soilto the shoots and stem nodules of S. rostrata. Time-course experimentsare described that investigate the movement of iron throughdifferent plant parts, detailing the route of iron translocation.The results show that iron moves first to the mature leavesand is subsequently translocated to the vegetative buds andstem nodules. Phloem girdling the stem nodules almost completelystops the movement of labelled iron into the nodules. We concludethat iron is supplied to stem nodules in the phloem; these resultssupport other work suggesting there is no inward xylem flowinto legume nodules, which probably receive almost all nutrientsand most of their water via phloemmediated transport. Key words: Translocation, nutrient, Fe, iron, stem nodules, legume, nitrogen fixation     

The Effects of Mechanically-induced Stress on the Growth of Water and Nutrient Deficient Lettuce and Cauliflower Seedlings

Mechanically-induced stress (MIS) applied by brushing the shootsof lettuce and cauliflower seedings with paper for 90 s eachday retarded the growth of water deficient and nutrient deficientseedlings as effectively as it did the growth of those wateredregularly or fed regularly with nutrient. The results are discussedboth in relation to how MIS might effect plant growth in thefield and to the possible use of stress treatments applied duringthe raising of transplants. Lactuca sativa L., lettuce, Brassica oleracea var, botrytis DC, cauliflower, mechanically-induced stress (MIS), water deficiency, nutrient deficiency, water potential     

Growth Consequences of Soil Nutrient Heterogeneity for two Old-field Herbs,Ambrosia artemisiifoliaandPhytolacca americana, Grown Individually and in Combination

Plant species can respond to small scale soil nutrient heterogeneityby proliferating roots or increasing nutrient uptake kineticsin nutrient-rich patches. Because root response to heterogeneitydiffers among species, it has been suggested that the distributionof soil resources could influence the outcome of interspecificcompetition. However, studies testing how plants respond toheterogeneity in the presence of neighbours are lacking. Inthis study, individuals of two species,Phytolacca americanaL.andAmbrosia artemisiifoliaL. were grown individually and incombination in soils with either a homogeneous or heterogeneousnutrient distribution. Above-ground biomass of individuallygrown plants of both species was greater when fertilizer waslocated in a single patch than when the same amount of fertilizerwas distributed evenly throughout the soil. Additionally, bothspecies proliferated roots in high-nutrient patches.A. artemisiifoliaexhibitedlarger root:shoot ratios, increased nitrogen depletion fromnutrient patches, and a higher growth rate thanP. americana,suggestingA. artemisiifoliais better suited to find and rapidlyexploit nutrient patches. In contrast to individually grownplants, soil nutrient distribution had no effect on final above-groundplant biomass for either species when grown with neighbours,even though roots were still concentrated in high nutrient patches.This study demonstrates that increased growth of isolated plantsas a consequence of localized soil nutrients is not necessarilyan indication that heterogeneity will affect interspecific encounters.In fact, despite a significant below-ground response, soil nutrientheterogeneity was inconsequential to above-ground performancewhen plants were grown with neighbours.Copyright 1999 Annalsof Botany Company Phytolacca americana, pokeweed,Ambrosia artemisiifolia, ragweed, nutrient heterogeneity, root proliferation, plasticity, foraging, nutrient patches.     

Interaction Between Mineral Nutrients, Cytokinin and Gibberellic Acid During Growth of Sorghum at High NaCI Salinity

Sorghum bicolor (L.) Moench, cv. 610, adapted to high salinitywas able to grow at 300 mol m^–3 NaCl only when half-strengthHoagland's solution was enriched with mineral nutrients. Theoptimal growth rate was observed in full strength Hoagland'ssolution; at higher or lower concentrations growth rates werelower. In contrast, growth rate of plants exposed to 150 molm^–3 NaCl was not affected by similar modification of theHoagland solution concentration. At high salinity, additionof cytokinin (CK) or gibberellic acid (GA), or a mixture ofboth, can induce the same effect on growth as the increasedmineral nutrient concentration. Phytohormones and increasedmineral concentration have similar effects, possibly becausean imbalance in phytohormones, rather than a mineral deficiency,limits growth at 300 mol m^–3 NaCl in the presence of half-strengthHoagland solution. The change in mineral concentration in thenutrient medium, in addition to its nutritional effect, alsoapparently acts as a signal involved in hormonal balance whichallows growth at high salinity. Exposure of Sorghum to 300 molm^–3 NaCl causes a decrease in the range of nutrient concentrationswhich can sustain growth. Adjustment of the nutrient concentrationmay induce the synthesis of endogenous CK and GA concentrationsrequired for growth. In contrast, addition of CK or GA at similarconcentrations during the adaptation (pretreatment) period inhibitsgrowth and prevents the adaptation process. The response tothe exogenous phytohormone treatments depends on the time elapsedfrom the beginning of salinization. Key words: Adaptation to salinity, cytokinin, gibberellic acid, mineral nutrition, growth, Sorghum, NaCl     

Sensing Iron--A Whole Plant Approach

Regulatory mechanisms leading to cellular Fe homeostasis wereinvestigated inPlantago (Plantago lanceolata L.) plants grownhydroponically at different temperature regimes either in thepresence or absence of iron. During the experimental periodof 6 d, growth was not affected by Fe availability, but wasdecreased by lowering the root zone temperature (RZT) from 24to 12°C. Cultivating plants at low RZT decreased the reductionactivity for ferric chelates in Fe-deficient plants. In thepresence of iron, the temperature regime did not affect Fe accumulationby root cells, but decreased translocation of Fe to the shoot,and chlorosis of young leaves was observed at suboptimal RZT.Under these conditions root-mediated reduction of ferric chelateswas increased. In cold-treated plants this effect was specificto Fe and could not be evoked by Mn²⁺and Zn ^{+ 2}additions. Supplementingthe medium with the ferrous scavenger ferrozine caused a furtherenhancement in reduction rates, probably due to mobilizationof apoplastic Fe. These results can be explained plausibly ifdifferent sites of Fe sensing are postulated and if it is assumedthat both the absence and presence of iron could be a signalincreasing root reduction activity. Copyright 2000 Annals ofBotany Company Adaptation, iron uptake regulation, ferric reduction, Plantago lanceolata, root zone temperature, whole plant signalling     

Nutrient Relations of Groundsel (Senecio vulgaris) Infected by Rust (Puccinia lagenophorae) at a Range of Nutrient Concentrations I. Concentrations, Contents and Distribution of N, P and K

Groundsel (Senecio vulgaris L.), healthy or infected with therust fungus Puccinia lagenophorae was grown in sand and fedwith a complete nutrient medium diluted to give a range of concentrations.Analysis of bulked, dried tissues of the plant showed that undernutrient-rich conditions rust infection resulted in increasedconcentrations of total (Kjeldahl) nitrogen and potassium buthad little effect on phosphorus concentration. Thus, despitereduced dry weight growth, total plant nitrogen contents wereno less in rusted than control plants. Although total contentsof phosphorus and potassium were reduced by rust, effects wereprobably related to loss of these nutrients in fungal spores. Interactions between rust infection and nutrient supply weresignificant but differed between nutrients: rust caused increasednitrogen concentrations only under nutrient-rich conditionsbut increased phosphorus concentrations only when nutrient supplywas limited. Increased concentrations were not confined to infectedtissues. Mechanisms underlying rust-nutrient interactions appearto be complex and to depend inter alia on the partitioning andrecycling of the particular nutrient within the plant. Rust-inducedincreases in potassium concentration occurred under both highand low nutrient conditions but were confined to infected tissues.Potassium accumulation in nutrient deficient conditions wasprobably due to increased transpirational flux into infectedtissues, but under nutrient-rich conditions reduced potassiumexport appeared to assume greater significance. The possible implications of the changed nutrient relationsfor the wider interactions of rust-infected plants in naturalvegetation are discussed. Senecio vulgaris, Puccinia lagenophorae, rust infection, nutrient deficiency, nutrient content, nutrient concentration, nutrient distribution     

Cluster Roots in Casuarinaceae: Role and Relationship to Soil Nutrient Factors

N₂-fixing actinorhizal trees in the family Casuarinaceae areeconomically of great interest in tropical and sub-tropicalzones because they are used for many purposes including protectionagainst wind, stabilization of sand dunes and the productionof firewood and charcoal. They are usually able to grow in sandysoils with low fertility by virtue of their ability to fix N₂.The objective of this review is to discuss briefly the roleof mycorrhizas and, more extensively, that of cluster (proteoid)roots, developed by a number of species of Casuarinaceae toimprove the absorption of nutrients other than N from soil,especially those needed for N₂fixation and growth. After evaluatingthe actual relationships between mycorrhizas and the Casuarinaceae,we highlight the possible role of cluster roots as an effectivealternative to mycorrhizas, and as a means of improvement ofgrowth of the trees in nutrient-deficient soils. This raisesthe question of what triggers the formation of cluster rootsin the Casuarinaceae. In addition to phosphorus deficiency,iron deficiency seems to be a major factor inducing the formationof cluster roots in Casuarina glauca and C. cunninghamiana.The number of cluster roots and the precocity of their formationare directly related to plant chlorosis due to Fe deficiency,as expressed by the critical concentration of chlorophyll inthe shoot (0.60 mg g ^{- 1}shoot f.wt). The effect of the nitrogensource on cluster root formation is discussed in relation topH values in the plant culture solution. The number of clusterroots formed in nitrate-fed plants increases with pH in therange of 5 to 9. Experiments carried out with alkaline and acidicsoils show that cluster roots are only produced when they areneeded to overcome soil nutrient deficiency due to the immobilizationof nutrient elements (P and Fe) by soil alkalinity. The possibleinvolvement of ethylene in the initiation and/or the morphogenesisof cluster roots is discussed. Copyright 2000 Annals of BotanyCompany Casuarinaceae, Casuarina cunninghamiana, Casuarina glauca, cluster roots, ethylene, iron deficiency, phosphorus deficiency, proteoid roots     

Effect of Some Chelating Agents on Growth and Antheridial Production in Male Clones of Three Mosses Grown In Vitro

The present work deals with the effect of the chelating agentsethylenediamine-di(o-hydroxyphenylacetic acid) [EDDHA] ethylenediamine tetra acetic acid [EDTA] and their iron salts, salicylicacid and ferric citrate on growth and antheridial productionin male clones of three dioecious mosses: Barbula gregaria,Bryum coronatum and Philonotis turneriana., Barbula and Bryumdevelop antheridia in ordinary cultural conditions on basalmedium, whereas Philonotis remains sterile. In Barbula and BryumEDDHA and EDTA enhance the percentage of fertile gametophytes.Fe-EDDHA and Fe-EDTA increase antheridial production as wellas vegetative growth and the former effect is more striking.In this respect Fe-EDDHA is better than Fe-EDTA for Barbula,whereas the reverse is true for Bryum. Salicylic acid inhibitsantheridial production and vegetative growth. Ferric citrateenhances vegetative growth in all three mosses. In Barbula andBryum it also stimulates antheridial production, and this effectis more marked than that on vegetative growth. None of the chelatestested induces antheridia in Philonotis. antheridial production, Barbula gregaria (Mitt.) Jaeg., Bryum coronatum Schwaegr., Philonotis turneriana (Schwaegr.) Mitt., mosses, chelating agents, bryophyta     

Floral Induction in Wolffia microscopica by Salicylic Acid and Related Compounds under Non-inductive Long Days

Flowering in Wolffia microscopica, a short-day plant, couldbe induced with salicylic acid (SA), under long days. Aspirin,benzoic acid and salicylaldoxime were also effective for inductionof flowering in this duckweed. Amonsgt these, SA is the mosteffective compound, as it could induce flowering even at 10^–7M. Flowering was further enhanced when Wolffia fronds were subjectedto short days, in the presence of SA. However, SA neither showedany effect on flowering ofW. microscopica in the absence ofEDTA in the nutrient medium, nor could it, by itself, supporteven the vegetative growth. The probable mechanism of actionof SA has also been discussed. It appears that the effect cannotbe due simply to chelation of metal ions and perhaps the salicylmoiety itself exerts a specific effect. (Received March 15, 1983; Accepted May 6, 1983)     

Influence of two Daphnia species on summer phytoplankton assemblages from eutrophic lakes

We conducted grazing experiments to test whether larger-bodiedDaphnia pulicaria have a different effect from smaller-bodiedDaphnia galeata mendotae on the composition of summer algalassemblages in eutrophic lakes. Three separate cubitainer experimentswere run for 5 days in a replicated factorial design utilizingtwo algal community types and the two Daphnia species. Inorganicphosphorus and nitrogen were added to prevent nutrient limitationof the algae. Both edible and inedible size fractions of chlorophylla increased in cubitainers without Daphnia spp. Grazer additionusually resulted in a reduction in edible chlorophyll; reductionswere greater in D.pulicaria cubitainers. Grazing by Daphniaspp. on presumed inedible chlorophyll was variable. Algal sizewas not always a good predictor of grazeability. The resultsof this study indicate that D.pulicaria, because of its greaterfiltration potential and ability to ingest larger particles,provides a stronger control on inedible-sized algae when comparedto equal numerical densities of D.g.mendotae. However, Aphanizomenonincreased as a response to heavy grazing pressure by D.pulicariaon other algal species. This suggests that biomanipulation effortsthat promote large-bodied Daphnia may not produce desirableresults if nutrient inputs remain high.     

The Effect of Boron on the Breaking, and Possible Control of Dormancy of Seed of Themeda triandra Forsk

Dormancy of the caryopsis of Themeda triandra Forsk. was shownto be broken by the addition of boron to the germinating media.Further boron was found to stimulate the germination processes.This effect was specific for boron, as no response was observedwith zinc, copper, manganese, iron, cobalt, and molybdenum inthe germinating medium. Evidence is put forward suggesting thatdormancy is regulated by the state of the endogenous boron inthe caryopsis. Gibberellic acid was also found to break dormancy,but the effect of gibberellic acid was never as great as thatobserved with boron. Maximum effect was observed in the presenceof boron and gibberellic acid together in the medium. The relationships between boron and gibberellic acid are discussed.     

Protonemal Morphogenesis of the Moss Tetraphis pellucida Hedw. in Culture and in the Wild

In axenic culture, the protonemal filaments of Tetraphis pellucidaderived from either spores and gemmae, or excised stems andleaves, share a mixture of attributes of chloronemata and caulonemata.Indirect immunofluorescence reveals that the tip cells containcortical and endoplasmic arrays of microtubules at interphase,and phragmoplasts associated with cell plate formation, butpre-prophase bands are absent. Protonemal plates originate fromthe same sites as filamentous protonemal side branches or directlyfrom young gemmae or excised stem fragments. These plates havea cylindrical base, the latter producing a single gametophorebud, and a unistratose lamina. The gametophores produce gemmacups in culture with the vegetative life cycle taking approximately28 d. Gibberellic acid (GA₃) has no visible effect on protonemal morphogenesiswhereas the auxin naphthalene acetic acid (NAA) suppresses plateand side branch formation. In the presence of kinetin the platesare callus-like and produce supernumerary buds. Abscisic acid(ABA) induces malformed plates and filaments with swollen cells,similar to those found in ageing cultures. Rhizoids are produced in abundance from gametophores and protonemalplates in nature but were never seen in culture. In the wild,rhizoids produce numerous protonemal plates and occasional gametophorebuds. The former are the main source of new shoots. The filamentousprotonemal phase in nature mainly comprises upright filamentscontaining one or more abscission cells. The protonemal plates in Tetraphis are homologous with thosein the allied genus Tetrodontium but are very different fromthose in Diphyscium and Sphagnum. Differences between cultureand nature are attributed to lower nutrient levels and irradiancesin the wild. Tetraphis pellucida, protonema, moss, morphogenesis, immunocytochemistry, gemmae, tip growth, vegetative reproduction     

On the Physiological Significance of Seminal Roots in Perennial Grasses

Measurements of yield and composition of plants of timothy (PhleumparatenseL.) show that nutrient uptake and growth are restricted if nutrientsare supplied only to the seminal roots. Plants whose nutrientswere supplied entirely through their adventitious roots didnot differ significantly in any of the characters studied fromthose in which all the roots had a nutrient supply available. It is therefore concluded that seminal roots have no specialphysiological significance.