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1.
When a Euglena, in a medium containing ATP, is microinjected with 7 × 10−14 l of 0.02 M EDTA, which binds Ca2+ and Mg2+, flagellar motility stops. Flagellar arrest in Chlamydomonas occurs with the injection of 2 × 10−14 l of 0.02 M EDTA. The injection of similar amounts (7 × 10−14 l in Euglena and 3 × 10−14 l in Chlamydomonas) of 0.02 M EGTA, which preferentially binds Ca2+, did not significantly alter flagellar motility. This suggests that a decrease in the internal Ca2+ concentration in Euglena or Chlamydomonas did not stimulate flagellar beating. Further, flagellar motility decreased when internal Mg2+ was chelated. The microinjection of Zn2+ into these cells caused a decrease in flagellar frequency analogous to the decrease in frequency caused by the injection of Ca2+ and EDTA. The microinjection of 7 × 10−14 l of 0.2 M Mn2+ caused an approx. 1.5-fold increase in Euglena flagellar motility. Chlamydomonas flagella, which cease to beat upon impalement in an Mg2+-free medium, resume a flagellar frequency of 18 Hz when injected with 3 × 10−14 l of 0.2 M Mn2+. In the experiments reported here, Mn2+ acts as an analog of Mg2+. 相似文献
2.
Summary A short cylindrical pocket arises as an infolding from the ventral surface of the reservoir near the canal in several species ofEuglena (E. mutabilis, E. gracilis strain T,E. spec.). The structure is linked to a band of microtubules which is shown to be identical to the ventral flagellar root of the euglenoid flagellar root system. An absolute configuration analysis of the flagellar root system inE. mutabilis and a comparison with the flagellar apparatus of colourlessEuglenophyceae and the bodonids (Kinetoplastida) reveals structural and positional homology between the reservoir pocket ofEuglena and the cytostome of these organisms and strongly supports the phylogenetic derivation of theEuglenophyceae from theKinetoplastida and the evolution of greenEuglenophyceae from phagotrophic colourless taxa. The functional significance of the cryptic cytostome ofEuglena is discussed in relation to the occurrence of intracellular endosymbiotic bacteria. 相似文献
3.
《The Journal of cell biology》1980,84(2):355-363
The flagellar frequency and waveform of Euglena were analyzed under full illumination (420-700 nm) and in a restricted wavelength band (530- 700 nm) when the cells were in a medium containing Mg2+ or had been microinjected with Mg2+, Mn2+, or Ca2+ in solution. Magnesium abolished the change in flagellar frequency and the reversal in waveform that cells exhibit when illuminated by a 530-700 nm wavelength band. Under this restricted illumination, Ca2+ caused an increase in flagellar waveform reversal and a decrease in beating frequency. The flagellar motility of cells impaled on a microelectrode was examined in cells illuminated with various wavelengths. 相似文献
4.
When a Euglena, in a medium containing ATP, is microinjected with 7 × 10?14 l of 0.02 M EDTA, which binds Ca2+ and Mg2+, flagellar motility stops. Flagellar arrest in Chlamydomonas occurs with the injection of 2 × 10?14 l of 0.02 M EDTA. The injection of similar amounts (7 × 10?14 l in Euglena and 3 × 10?14 l in Chlamydomonas) of 0.02 M EGTA, which preferentially binds Ca2+, did not significantly alter flagellar motility. This suggests that a decrease in the internal Ca2+ concentration in Euglena or Chlamydomonas did not stimulate flagellar beating. Further, flagellar motility decreased when internal Mg2+ was chelated. The microinjection of Zn2+ into these cells caused a decrease in flagellar frequency analogous to the decrease in frequency caused by the injection of Ca2+ and EDTA. The microinjection of 7 × 10?14 l of 0.2 M Mn2+ caused an approx. 1.5-fold increase in Euglena flagellar motility. Chlamydomonas flagella, which cease to beat upon impalement in an Mg2+-free medium, resume a flagellar frequency of 18 Hz when injected with 3 × 10?14 l of 0.2 M Mn2+. In the experiments reported here, Mn2+ acts as an analog of Mg2+. 相似文献
5.
The organization of two types of nontubular mastigonemes associated with the anterior flagellar surface of the phagotrophic biflagellate Peranema trichophorum (Ehrenberg) Stein is described from studies of thin sections, negative-stained and shadow-cast preparations of both intact and isolated, detergent-treated flagella. Long mastigonemes form a unilateral, spiral array of tufts which curve toward the distal end of the flagellum, while two short mastigoneme ribbons form unequal halves of a bilateral array parallel to the flagellar long axis. Each ribbon is composed of individual overlapping fan-shaped tiers of short mastigonemes interlinked by fine fibrils. A model proposed for Peranema mastigonemes is similar to recent models of mastigoneme organization in Euglena. 相似文献
6.
Tubulin Genes in the Algal Protist Euglena gracilis 总被引:1,自引:0,他引:1
PIERRE J. LEVASSEUR QI MENG G. BENJAMIN BOUCK 《The Journal of eukaryotic microbiology》1994,41(5):468-477
ABSTRACT Alpha- and beta-tubulin cDNA were selected from a Euglenaλgt11 expression library, recloned and either sequenced (α-tubulin cDNA) or hybridized to Euglena RNA and DNA (α- and β-tubulin cDNA). RNA for hybridization was extracted at 30 minute intervals after flagellar amputation and quantitated for cDNA binding. Unlike previous reports on most other flagellates, no net increase in either α- or β-tubulin RNA could be detected during regeneration—suggesting steady state or constitutive tubulin RNA synthesis. Incubation of the cDNA with genomic DNA after restriction digestion produced patterns of hybridization consistent with the presence of one to two kinds each of the α- and β-tubulin genes. The deduced amino acid sequence of the α-tubulin cDNA was more than 90% identical to the α-tubulins of Trypanosoma, Chlamydomonas, Naegleria, Tetrahymena and higher plants. The carboxy terminus of the α-tubulin cDNA and the previously sequenced β-tubulin of Euglena showed greatest identity to the carboxy terminus of the tubulins from Trypanosoma brucei. The sequence data for α and β-tubulins of Euglena provides direct evidence for the similarity of two gene products from euglenas and trypanosomes and adds support to earlier suggestions that these organisms are phylogenetically related. 相似文献
7.
The pigments associated with the flagellum of the phytoflagellateEuglena gracllis were characterized by HPLC. The pigment pattern of the wild-type strain was compared with a set of white mutants which did
not display phototaxis and photoaccumulation in response to blue light. Flagella of the wild type contained FMN and FAD. Two
mutants which lacked the stigma but retained a small paraxonemal body (PAB) contained less flavins. The whiteEuglena mutant FB, which retained a residual stigma and also a PAB, and the white phytoflagellateAstasia longa, a close relative ofEuglena, had normal amounts of flagellar flavins. Cells and flagella ofEuglena wild type contained an unldentified pterin-like pigment, called Pt16, which was substantially reduced inAstasia and theEuglena mutants. A third pigment, designated P528 with major absorption at 528 nm and fluorescence emission at 550 nm was present mainly in flagella. The association of the
three pigment types with flagella and their respective alterations in the white strains indicates their possible role in photoreception.
Dedicated to Pill-Soon Song on the occasion of his 60th birthday. 相似文献
8.
Flagellar activity in the biflagellate chlorophyte Chlamydomonas reinhardtii is selectively inhibited by Ni2+ or by treatment with Ca2+-chelating agents. Inhibitions of swimming speed, geotaxis, phototaxis, and pattern swimming result from qualitative and quantitative losses in the activity of individual flagella and in the coordination of activity beween the 2 flagella of each cell. Addition of Ca2+ (a) prevents inhibition and (b) restores normal flagellar activity in inhibited cells. Mg2+ is partially effective in reversal of inhibition. Other ions do not cause similar inhibition or reversal of nickel inhibition. The characteristics of inhibition and reversal suggest that the prmary target for nickel is a component of the flagellar apparatus, and that this component uses Ca2+ to perform its normal function in the regulation of flagellar activity. A 2nd target for nickel is a Carequiring process specific to phototaxis (and not involved in the photophobic response). 相似文献
9.
《Micron and Microscopica Acta》1992,23(1-2):37-44
euglena is a mobile photosynthetic and photosensitive cell which dwells in fresh water. Euglena has an important evolutionary role, therefore its morphology has been and still is a subject of interest. The structures in Euglena which are involved in photoreception and signal transduction and transmission have been well studied. In this paper morphological and ultrastructural evidence of a single stable structure formed by the photoreceptor (paraflagellar swelling) and the other specialized flagellar structures of Euglena gracilis (paraxial rod) is provided using conventional and non-conventional SEM and TEM, on detergent treated cells, and on isolated flagella retaining their photoreceptor. 相似文献
10.
The flagella of populations of three protozoan species (Ochromonas, Euglena, and Astasia) were amputated and allowed to regenerate. The kinetics of regeneration in all species were characterized by a lag phase during which there was no apparent flagellar elongation; this phase was followed by elongation at a rate which constantly decelerated as the original length was regained. Inhibition by cycloheximide applied at the time of flagellar amputation showed that flagellar regeneration was dependent upon de novo protein synthesis. This was supported by evidence showing that a greater amount of leucine was incorporated into the proteins of regenerating than nonregenerating flagella. The degree of inhibition of flagellar elongation observed with cycloheximide depended on how soon after flagellar amputation it was applied: when applied to cells immediately following amputation, elongation was almost completely inhibited, but its application at various times thereafter permitted considerable elongation to occur prior to complete inhibition of flagellar elongation. Hence, a sufficient number of precursors were synthesized and accumulated prior to addition of cycloheximide so that their assembly (elongation) could occur for a time under conditions in which protein synthesis had been inhibited. Evidence that the site of this assembly may be at the tip of the elongating flagellum was obtained from radioautographic studies in which the flagella of Ochromonas were permitted to regenerate part way in the absence of labeled leucine and to complete their regeneration in the presence of the isotope. Possible mechanisms which may be operating to control flagellar regeneration are discussed in light of these and other observations. 相似文献
11.
Summary The role of tubular mastigonemes in the reversal of thrust of the anterior flagellum ofPhytophthora cinnamomi was analysed using mastigoneme-specific monoclonal antibodies and immunoflu-orescence and video microscopy. Exposure of live zoospores ofP. cinnamomi to the mastigoneme-specific Zg antibodies caused alterations in the arrangement of mastigonemes on the flagellar surface and at Zg concentrations above 0.3 /ml, mastigonemes became detached from the flagellum. As a consequence of antibody binding to the mastigonemes there were concentration-dependent perturbations in zoospore swimming behaviour and anterior flagellum beat pattern. With increasing antibody concentration zoospores swam more slowly and other parameters of their swimming pattern, such as the wavelength of the swimming helix and the frequency of rotation, were also reduced. The effects of Zg antibodies were specific at two levels: control immunoglobulins or antibodies that bound to other flagellar surface components did not have an effect on motility, and Zg antibodies did not interfere with the motility of zoospores of oomycete species to which they did not bind. The effects of antibody-induced disruption of mastigoneme arrangement strongly support previous hypotheses that tubular mastigonemes are responsible for thrust reversal by the anterior flagellum, enabling it to pull the cell through the surrounding medium. 相似文献
12.
Euglena viridis (subgenus Euglena) serves as the type species for the genus Euglena. In this study, molecular phylogenetic analyses using a small subunit (SSU) and a combined SSU–partial large subunit rDNA data set for members of the genus Euglena showed that strains identified as E. viridis on the basis of morphology are distributed between two separate nonsister clades. Although all the E. viridis strains examined were morphologically indistinguishable and possessed spherical mucocysts and stellate chloroplasts with one paramylon center, there was a high degree of sequence divergence between the E. viridis strains in different clades, making this a cryptic species. Like E. viridis, all taxa from the subgenus Euglena are characterized by having one or more stellate chloroplasts with paramylon grains clustered around the center of the chloroplast. These additional taxa were divided into four clades in all the molecular analyses. Strains of Euglena stellata formed two nonsister clades whose members had a single aggregate chloroplast with paramylon center and spindle‐shaped mucocysts. A geniculata clade included species with one or two stellate chloroplasts with paramylon centers and spherical mucocysts, and the cantabrica clade had members with one stellate chloroplast with paramylon center and spherical mucocysts often arranged in spiral rows. Interspersed among these were three additional clades bearing taxa from the subgenus Calliglena that contains members with discoid plastids and pyrenoids that may or may not be capped with paramylon. These taxa formed a laciniata clade, mutabilis clade, and gracilis clade. This study demonstrates that E. viridis and E. stellata are cryptic species that can only be distinguished at the molecular level. Because E. viridis is the designated type species for the genus Euglena, we designated an epitype for E. viridis. 相似文献
13.
The freshwater green euglenoid Euglena anabaena var. minor has a pellicle with groove‐ridge articulation, a chloroplast with pyrenoids doubly sheathed by two paramylon caps, and a nucleus with permanently condensed chromosomes and nucleolus. The flagellar apparatus basically resembles that of Euglena. The dorsal root (DR) originates at the dorsal basal body of the emergent flagellum, while both the intermediate root (IR) and ventral root (VR) originate at the ventral basal body of the non‐emergent flagellum. The cytoplasmic pocket is associated with the ventral root/ reinforcing microtubular band. However, ultrastructural characterization of E. anabaena var. minor shows the pocket to consist of five to seven microtubules, and flagellar roots with microtubule configuration of 3–4–6 in the DR‐IR‐VR. The dorsal band microtubules pair at the reservoir‐canal transition level. The doublet microtubules are formed into triplets and doublets at the lower canal level and then make pellicular microtubules at the upper canal level. 相似文献
14.
《Journal of microbiological methods》1988,8(6):327-332
Practical criteria for the usefulness of an algal separation process for laboratory routine are effectiveness and time consumption. We tested the feasibility of a flocculation procedure for harvesting a large volume of Euglena gracilis culture. This procedure turned out to be a technically viable system for avoiding tedious centrifugation and preserving Euglena flagellar apparatus integrity.Euglena cultures were treated with chitosan, a byproduct derived from chitin, the major component of the exoskeleton of crustaceans. Since chitosan carries a positive chrge, it functions as a polycationic coagulating agent by adsorbing onto particles in suspension and by bridging into agglomerates or flocs. A 96–98% reduction in the number of suspended cells was obtained in the cultures with 200 mg/l of chitosan at pH 7.5. 相似文献
15.
Summary Five peaks of RNA from bleached Euglena gracilis are resolved by polyacrylamide-gel electrophoresis. The extraction of these RNA's and their subsequent resolution on gels is dependent upon pH and the presence of an RNase inhibitor (e.g., macaloid). Careful control of ionic strength also appears necessary. Inorganic phosphate is incorporated first by low molecular weight RNA, then by a high molecular weight RNA (hRNA) and a peak with a sedimentation coefficient of 13S, and then by rRNA.The electrophoretic pattern of RNA from Astasia longa is similar to that of bleached Euglena whereas that from wild-type Euglena is more complex and presumably reflects the presence of chloroplast RNA's in these latter cells. 相似文献
16.
Summary Low temperature spectra are described for whole Euglena cells. Euglena growing in synchronous culture with lactate medium show a cyclic variation of cytochrome 556 content during each cellular generation. The greatest quantity of cytochrome 556 seems to coincide with the non-dividing phase of the cells, the phase in which the mitochondrial network is observed. On the other hand after treatment of the Euglena with antimycin A, a correlation exists between the formation of giant mitochondria and an increase in the quantity of cytochrome 556.These results demonstrate the existence of a cyclic variation of cytochrome 556 synthesis in Euglena during synchronous growth on lactate medium. 相似文献
17.
Dolly K. Khona Venkatramanan G. Rao Mustafa J. Motiwalla P. C. Sreekrishna Varma Anisha R. Kashyap Koyel Das Seema M. Shirolikar Lalit Borde Jayashree A. Dharmadhikari Aditya K. Dharmadhikari Siuli Mukhopadhyay Deepak Mathur Jacinta S. D’Souza 《Journal of biological physics》2013,39(1):1-14
Chlamydomonas reinhardtii has long been used as a model organism in studies of cell motility and flagellar dynamics. The motility of the well-conserved ‘9+2’ axoneme in its flagella remains a subject of immense curiosity. Using high-speed videography and morphological analyses, we have characterized long-flagella mutants (lf1, lf2-1, lf2-5, lf3-2, and lf4) of C. reinhardtii for biophysical parameters such as swimming velocities, waveforms, beat frequencies, and swimming trajectories. These mutants are aberrant in proteins involved in the regulation of flagellar length and bring about a phenotypic increase in this length. Our results reveal that the flagellar beat frequency and swimming velocity are negatively correlated with the length of the flagella. When compared to the wild-type, any increase in the flagellar length reduces both the swimming velocities (by 26–57%) and beat frequencies (by 8–16%). We demonstrate that with no apparent aberrations/ultrastructural deformities in the mutant axonemes, it is this increased length that has a critical role to play in the motion dynamics of C. reinhardtii cells, and, provided there are no significant changes in their flagellar proteome, any increase in this length compromises the swimming velocity either by reduction of the beat frequency or by an alteration in the waveform of the flagella. 相似文献
18.
IC138 is a WD-repeat dynein intermediate chain required for light chain assembly and regulation of flagellar bending
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Hendrickson TW Perrone CA Griffin P Wuichet K Mueller J Yang P Porter ME Sale WS 《Molecular biology of the cell》2004,15(12):5431-5442
Increased phosphorylation of dynein IC IC138 correlates with decreases in flagellar microtubule sliding and phototaxis defects. To test the hypothesis that regulation of IC138 phosphorylation controls flagellar bending, we cloned the IC138 gene. IC138 encodes a novel protein with a calculated mass of 111 kDa and is predicted to form seven WD-repeats at the C terminus. IC138 maps near the BOP5 locus, and bop5-1 contains a point mutation resulting in a truncated IC138 lacking the C terminus, including the seventh WD-repeat. bop5-1 cells display wild-type flagellar beat frequency but swim slower than wild-type cells, suggesting that bop5-1 is altered in its ability to control flagellar waveform. Swimming speed is rescued in bop5-1 transformants containing the wild-type IC138, confirming that BOP5 encodes IC138. With the exception of the roadblock-related light chain, LC7b, all the other known components of the I1 complex, including the truncated IC138, are assembled in bop5-1 axonemes. Thus, the bop5-1 motility phenotype reveals a role for IC138 and LC7b in the control of flagellar bending. IC138 is hyperphosphorylated in paralyzed flagellar mutants lacking radial spoke and central pair components, further indicating a role for the radial spokes and central pair apparatus in control of IC138 phosphorylation and regulation of flagellar waveform. 相似文献
19.
Steering their swimming direction toward the light is crucial for the viability of Volvox colonies, the larger members of the volvocine algae. While it is known that this phototactic steering is achieved by a difference in behavior of the flagella on the illuminated and shaded sides, conflicting reports suggest that this asymmetry arises either from a change in beating direction or a change in beating frequency. Here, we report direct observations of the flagellar behavior of various Volvox species with different phyletic origin in response to light intensity changes and thereby resolve this controversy: Volvox barberi W. Shaw from the section Volvox sensu Nozaki (2003) changes the direction of the flagellar beating plane, while species encompassed in the group Eudorina (Volvox carteri F. Stein, Volvox aureus Ehrenb., and Volvox tertius Art. Mey.) decrease the flagellar beating frequency, sometimes down to flagellar arrest. 相似文献
20.
Symmetry/asymmetry conversion of eukaryotic flagellar waveform is caused by the changes in intracellular Ca2+. Animal sperm flagella show symmetric or asymmetric waveform at lower or higher concentration of intracellular Ca2+, respectively. In Chlamydomonas, high Ca2+ induces conversion of flagellar waveform from asymmetric to symmetry, resulting in the backward movement. This mirror image relationship between animal sperm and Chlamydomonas could be explained by the distinct calcium sensors used to regulate the outer arm dyneins (Inaba 2015). Here we analyze the flagellar Ca2+-response of the prasinophyte Pterosperma cristatum, which shows backward movement by undulating four flagella, the appearance similar to animal sperm. The moving path of Pterosperma shows relatively straight in artificial seawater (ASW) or ASW in the presence of a Ca2+ ionophore A23187, whereas it becomes circular in a low Ca2+ solution. Analysis of flagellar waveform reveals symmetric or asymmetric waveform propagation in ASW or a low Ca2+ solution, respectively. These patterns of flagellar responses are completely opposite to those in sperm flagella of the sea urchin Anthocidaris crassispina, supporting the idea previously proposed that the difference in flagellar response to Ca2+ attributes to the evolutional innovation of calcium sensors of outer arm dynein in opisthokont or bikont lineage. 相似文献