共查询到20条相似文献,搜索用时 15 毫秒
1.
To screen stimulators from Chinese medicinal insects for mycelial growth and polysaccharides production of Ganoderma lucidum, G. lucidum was inoculated into the media with and without supplementation of medicinal insect extracts. The ethyl acetate extract of
Eupolyphaga sinensis at 55 mg l−1 lead to significant increase in both biomass and intracellular polysaccharides (IPS) concentration from 8.53 ± 0.41 to 14.16 ± 0.43
and 1.28 ± 0.09 to 2.13 ± 0.11 g l−1, respectively. In addition, the ethyl acetate extract of Catharsius molossus at 55 mg l−1 significantly enhanced extracellular polysaccharides (EPS) production; the EPS yield increased from 350.9 ± 14.1 to 475.1 ± 15.3 mg
l−1. There were no new components in the two types of polysaccharides obtained by the addition of the insect extracts. 相似文献
2.
Response surface methodology (RSM) was applied to optimize the critical medium ingredients of Agaricus blazei. A three-level Box–Behnken factorial design was employed to determine the maximum biomass and extracellular polysaccharide
(EPS) yields at optimum levels for glucose, yeast extract (YE), and peptone. A mathematical model was then developed to show
the effect of each medium composition and its interactions on the production of mycelial biomass and EPS. The model predicted
the maximum biomass yield of 10.86 g/l that appeared at glucose, YE, peptone of 26.3, 6.84, and 6.62 g/l, respectively, while
a maximum EPS yield of 348.4 mg/l appeared at glucose, YE, peptone of 28.4, 4.96, 5.60 g/l, respectively. These predicted
values were also verified by validation experiments. The excellent correlation between predicted and measured values of each
model justifies the validity of both the response models. The results of bioreactor fermentation also show that the optimized
culture medium enhanced both biomass (13.91 ± 0.71 g/l) and EPS (363 ± 4.1 mg/l) production by Agaricus blazei in a large-scale fermentation process. 相似文献
3.
Maple sap, an abundant natural product especially in Canada, is rich in sucrose and thus may represent an ideal renewable
feedstock for the production of a wide variety of value-added products. In the present study, maple sap or sucrose was employed
as a carbon source to Alcaligenes latus for the production of poly-β-hydroxybutyrate (PHB). In shake flasks, the biomass obtained from both the sap and sucrose were
4.4 ± 0.5 and 2.9 ± 0.3 g/L, and the PHB contents were 77.6 ± 1.5 and 74.1 ± 2.0%, respectively. Subsequent batch fermentation
(10 L sap) resulted in the formation of 4.2 ± 0.3 g/L biomass and a PHB content of 77.0 ± 2.6%. The number average molecular
weights of the PHB produced by A. latus from maple sap and pure sucrose media were 300 ± 66 × 103 and 313 ± 104 × 103 g/mol, respectively. Near-infrared, 1H magnetic resonance imaging (MRI), and 13C-MRI spectra of the microbially produced PHB completely matched those obtained with a reference material of poly[(R)-3-hydroxybutyric
acid]. The polymer was found to be optically active with [α]25
D equaled to −7.87 in chloroform. The melting point (177.0°C) and enthalpy of fusion (77.2 J/g) of the polymer were also in
line with those reported, i.e., 177°C and 81 J/g, respectively. 相似文献
4.
Hui Chen Mingchao Yan Jinwei Zhu Xiangqun Xu 《Journal of industrial microbiology & biotechnology》2011,38(2):291-298
We reported that lignocellulose decomposition can be used to facilitate the production of bioactive polysaccharides from submerged
culture of Inonotus obliquus. Exo-polysaccharide (EPS) production and antioxidant activity by Inonotus obliquus was enhanced by employing lignocellulose decomposition in a corn straw-containing submerged fermentation. A significant increase
in the EPS production and hydroxyl radical scavenging activity from 1.09 ± 0.01 g/l and 72.3 ± 1.9% in a basal medium to 1.38 ± 0.02 g/l
and 82.7 ± 0.5% in a corn straw-containing medium was obtained. A synchronized effect between lignocellulose decomposition
and malondialdehyde presenting hydroxyl radical concentration in the fermentation broth was identified. The adding of thiourea,
a hydroxyl radical-scavenging reagent, suppressed malondialdehyde generation and lowered the lignocellulose decomposition
rate. Correspondingly, the EPS production and hydroxyl radical scavenging activity decreased to 1.26 g/l and 74%. The EPS
obtained from the corn straw-containing medium also presented the strongest superoxide radical scavenging activity. The monosaccharide
components of the EPS from the corn straw-containing medium are rhamnose, arabinose, xylose, mannose, glucose, and galactose
with molar proportions at 3.0, 3.0, 0.9, 46.6, 11.4, and 35.1%, respectively, which are largely different from the molar proportions
of the EPS from the basal medium. 相似文献
5.
Qiao-Ning Liu Rui-Sang Liu Yi-Hua Wang Zhi-Yuan Mi Dong-Sheng Li Jian-Jiang Zhong Ya-Jie Tang 《Bioresource technology》2009,100(14):3644-3649
For the first time, a fed-batch fermentation process of Tuber melanosporum was developed for the efficient production of bioactive mycelia and Tuber polysaccharides. Each 1.67 g/L of peptone and 8.33 g/L of yeast extract were added on day 3, 6, and 9, respectively, and sucrose was fed to maintain its concentration around 35–5 g/L when its residual level decreased to 10–5 g/L. Then, the maximal biomass, the production of extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) reached 53.72 ± 2.57 g DW/L, 7.09 ± 0.62 and 4.43 ± 0.21 g/L, respectively. Compared with the batch culture conducted in the enriched medium, the biomass, the production of EPS and IPS were enhanced by 55.8%, 222.3% and 103.2%, respectively. Not only the cell density but also the production of EPS and IPS were the highest ever reported in truffle fermentation, and the biomass was also the highest as ever reported in mushroom fermentation. 相似文献
6.
Effects of Coix lacryma-jobi oil (CLO) addition on the mycelia growth and production of bioactive metabolites, such as triterpenoids, exopolysaccharide (EPS), and intracellular polysaccharide (IPS) in the submerged culture of Ganoderma lucidum were studied. The results showed that when a level of 2% CLO was added at the beginning of culture, the biomass, triterpenoids, EPS, and IPS productions reached a maximum of 10.71 g/L, 92.94 mg/L, 0.33 g/L, and 0.389 g/L, respectively, that were 3.34-fold, 2.76-fold, 2.2-fold, and 2.23-fold compared to that of control. Analysis of fermentation kinetics of G. lucidum suggested that glucose concentration in the culture of CLO-added group decreased more quickly as compared to the control group from day 2 to day 7 of fermentation process, while the triterpenoids and polysaccharides biosynthesis were promoted at the same culture period. However, the culture pH profile was not affected by the addition of CLO. There were no new components in the two types of polysaccharides obtained by the addition of CLO. Enzyme activities analysis indicated CLO or its fatty acids affected the synthesis level of phosphoglucose isomerase and α-phosphoglucomutase at different stage. 相似文献
7.
The mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (−) was systematically optimized in shake flasks. The ratio of the (+) to (−) strains, the lycopene
cyclase inhibitors piperidine and creatinine, the trisporic acid structural analog abscisic acid, the 3-hydroxy-3-methylglutaryl-coenzyme
A (HMG-CoA) precursor leucine, and the mevalonate kinase enhancer penicillin were all identified as key factors affecting
lycopene biosynthesis. With an optimal ratio of 5:1 for the (+) to (−) strains and the addition of 6 g/L creatinine on day
3, the highest lycopene production was 98.1 ± 15.5 mg/L. Based on the above result, the addition of 0.1 g/L penicillin on
day 4, 150 μmol/L abscisic acid on day 3 or 0.5 g/L leucine on day 4 enhanced lycopene production to 119.7 ± 17.2, 120.6 ± 12.3
and 135.2 ± 7.0 mg/L, respectively. Finally, an integrated strategy by combining the above key factors was developed, and
the highest lycopene production of 156.2 ± 15.4 mg/L was obtained, which was enhanced by 134.9% comparing with its production
of 66.5 ± 3.6 mg/L before the optimization process of this work. The results obtained in this study may be useful for large-scale
industrial lycopene production. 相似文献
8.
Hwang HS Lee SH Baek YM Kim SW Jeong YK Yun JW 《Applied microbiology and biotechnology》2008,78(3):419-429
In the present study, optimum culture conditions for the production of extracellular polysaccharides (EPS) in submerged culture
of an edible mushroom, Laetiporus sulphureus var. miniatus and their stimulatory effects on insulinoma cell (RINm5F) proliferation and insulin secretion were investigated. The maximum
mycelial growth (4.1 g l−1) and EPS production (0.6 g l−1) in submerged flask culture were achieved in a medium containing 30 g l−1 maltose, 2 g l−1 soy peptone, and 2 mM MnSO4·5H2O at an initial pH 2.0 and temperature 25°C. In the stirred-tank fermenter under optimized medium, the concentrations of mycelial
biomass and EPS reached a maximum level of 8.1 and 3.9 g l−1, respectively. Interestingly, supplementation of deep sea water (DSW) into the culture medium significantly increased both
mycelial biomass and EPS production by 4- and 6.7-fold at 70% (v/v) DSW medium, respectively. The EPS were proved to be glucose-rich polysaccharides and were able to increase proliferation
and insulin secretary function of rat insulinoma RINm5F cells, in a dose-dependent manner. In addition, EPS also strikingly
reduced the streptozotocin-induced apoptosis in RINm5F cells indicating the mode of the cytoprotective role of EPS on RINm5F
cells. 相似文献
9.
T Roukas 《Journal of industrial microbiology & biotechnology》1999,22(6):617-621
Aureobasidium pullulans P56 was investigated using an adaptation technique and a mixed culture system. The adaptation of A. pullulans and the mixed cultures of A. pullulans and/or Lactobacillus brevisX20, Debaryomyces hansenii 194 and Aspergillus niger did not increase the production of polysaccharide. Enzymic hydrolysis of lactose in deproteinized whey gave a higher polysaccharide
concentration and polysaccharide yield than acidic hydrolysed lactose. Maximum polysaccharide concentration (11.0 ± 0.5 g L−1), biomass dry weight (10.5 ± 0.4 g L−1), polysaccharide yield (47.2 ± 1.8%) and sugar utilization (93.2 ± 2.8%) were achieved using enzyme-hydrolysed whey (pH 6.5)
containing 25 g L−1 lactose and supplemented with K2HPO4 0.5%, L-glutamic acid 1%, olive oil 2.5%, and Tween 80 0.5%. In this case the pullulan content of the crude polysaccharide was 40%.
Received 16 December 1997/ Accepted in revised form 12 March 1999 相似文献
10.
Exopolysaccharide (EPS) production was compared among three strains of lactobacilli. Lactobacillus rhamnosus strain 9595M can be classified among the highest EPS-producing strains of lactic acid bacteria reported to date with a maximum
EPS production of 1275 mg L−1. Under controlled pH, no significant differences in the quantity of EPS produced could be detected between carbon source
(glucose or lactose) or fermentation temperature (32 or 37°C). In milk, strains ATCC 9595M and R produced more than 280 mg L−1 EPS whereas strain Type V produced less than 80 mg L−1 EPS. Journal of Industrial Microbiology & Biotechnology (2000) 24, 251–255.
Received 10 September 1999/ Accepted in revised form 22 December 1999 相似文献
11.
Cui J Goh KK Archer R Singh H 《Journal of industrial microbiology & biotechnology》2007,34(5):393-402
The protein-bound polysaccharides of Coriolus versicolor (CPS) have been reported to stimulate overall immune functions against cancers and various infectious diseases by activating
specific cell functions. A New Zealand isolate (Wr-74) and a patented strain (ATCC-20545) of C. versicolor were compared in this study. The fruit bodies of both strains were grown for visual verification. Both strains were grown
in submerged-culture using an airlift fermentor with milk permeate as the base medium supplemented with glucose, yeast extract
and salt. Metabolic profiles of both strains obtained over 7-day fermentation showed very similar trends in terms of biomass
production (8.9–10.6 mg/ml), amounts of extracellular polysaccharide (EPS) from the culture medium (1150–1132 μg/ml), and
intracellular polysaccharide (IPS) from the mycelium (80–100 μg/ml). Glucose was the dominant sugar in both EPS and IPS, and
the polymers each consisted of three molecular weight fractions ranging from 2 × 106 to 3 × 103 Da. Both the EPS and IPS were able to significantly induce cytokine production (interleukin 12 and γ interferon) in murine
splenocytes in vitro. Highest levels of interleukin 12 (291 pg/ml) and γ interferon (6,159 pg/ml) were obtained from samples
containing Wr-74 IPS (0.06 μg/ml) and ATCC 20545 IPS (0.1 μg/ml), respectively. The results indicated that lower levels of
EPS and IPS generally resulted in higher immune responses than did higher polymer concentrations. 相似文献
12.
Ethanol production by recombinant Escherichia coli strain FBR5 from dilute acid pretreated wheat straw (WS) by separate hydrolysis and fermentation (SHF) and simultaneous saccharification
and fermentation (SSF) was studied. The yield of total sugars from dilute acid (0.5% H2SO4) pretreated (160 °C, 10 min) and enzymatically saccharified (pH 5.0, 45 °C, 72 h) WS (86 g/l) was 50.0 ± 1.4 g/l. The hydrolyzate
contained 1,184 ± 19 mg furfural and 161 ± 1 mg hydroxymethyl furfural per liter. The recombinant E. coli FBR5 could not grow at all at pH controlled at 4.5 to 6.5 in the non-abated wheat straw hydrolyzate (WSH) at 35 °C. However,
it produced 21.9 ± 0.3 g ethanol from non-abated WSH (total sugars, 44.1 ± 0.4 g/l) in 90 h including the lag time of 24 h
at controlled pH 7.0 and 35 °C. The bioabatement of WS was performed by growing Coniochaeta ligniaria NRRL 30616 in the liquid portion of the pretreated WS aerobically at pH 6.5 and 30 °C for 15 h. The bacterium produced 21.6 ± 0.5 g
ethanol per liter in 40 h from the bioabated enzymatically saccharified WSH (total sugars, 44.1 ± 0.4 g) at pH 6.0. It produced
24.9 ± 0.3 g ethanol in 96 h and 26.7 ± 0.0 g ethanol in 72 h per liter from bioabated WSH by batch SSF and fed-batch SSF,
respectively. SSF offered a distinct advantage over SHF with respect to reducing total time required to produce ethanol from
the bioabated WS. Also, fed-batch SSF performed better than the batch SSF with respect to shortening the time requirement
and increase in ethanol yield. 相似文献
13.
Eliza Malinowska Wojciech Krzyczkowski Grzegorz Łapienis Franciszek Herold 《Journal of industrial microbiology & biotechnology》2009,36(12):1513-1527
The aim of this study was to optimize the culture medium used for the mycelial growth and production of intracellular polysaccharides
(IPS) and exopolysaccharides (EPS) in a submerged culture of Hericium erinaceum. Of the various factors examined, including carbon and nitrogen sources, vitamins, mineral elements, and initial pH, those
that proved to have a significant effect were then tested using a 24 central composite rotatable design (CCRD). Under the optimal culture conditions, the maximal yield of biomass reached 14.24 ± 0.45 g l−1 and was 1.85-fold higher than in the basal medium. The kinetics of EPS biosynthesis in a bioreactor showed that although
the highest yield of EPS (2.75 ± 0.27 g l−1) could be obtained on day 8, the process of biosynthesizing high molecular weight polysaccharides proceeded until the depletion
of the carbon source in the medium (after 14 days of cultivation). Our results could be very helpful in the large-scale production
of bioactive polysaccharides from H. erinaceum. 相似文献
14.
Daisuke Sugimori 《Applied microbiology and biotechnology》2009,82(2):351-357
To develop a microbial treatment of edible oil-contaminated wastewater, microorganisms capable of rapidly degrading edible
oil were screened. The screening study yielded a yeast coculture comprising Rhodotorula pacifica strain ST3411 and Cryptococcus laurentii strain ST3412. The coculture was able to degrade efficiently even at low contents of nitrogen ([NH4–N] = 240 mg/L) and phosphorus sources ([PO4–P] = 90 mg/L). The 24-h degradation rate of 3,000 ppm mixed oils (salad oil/lard/beef tallow, 1:1 w/w) at 20°C was 39.8% ± 9.9% (means ± standard deviations of eight replicates). The highest degradation rate was observed at
20°C and pH 8. In a scaled-up experiment, the salad oil was rapidly degraded by the coculture from 671 ± 52.0 to 143 ± 96.7 ppm
in 24 h, and the degradation rate was 79.4% ± 13.8% (means ± standard deviations of three replicates). In addition, a repetitive
degradation was observed with the cell growth by only pH adjustment without addition of the cells. 相似文献
15.
Adewale Adewuyi Rotimi A Oderinde B. V. S. K. Rao R. B. N. Prasad B. Anjaneyulu 《Bioenergy Research》2012,5(3):713-718
Self-sufficiency in energy requirement is critical to the success of any developing economy. Apart from the search for alternatives, there is a need to achieve energy independence, directing much focus on biofuels. Biodiesel is simple to use, biodegradable, nontoxic, and essentially free of sulfur and aromatics. Oil was extracted from the seeds of Blighia unijugata and Luffa cylindrica, subjected to chemical characterization and biodiesel production. The oil yield from the seed of B. unijugata was 50.82 ± 1.20% while that of L. cylindrica was 39.10 ± 0.20%. The biodiesel produced had ester content above 98%. The flash point of the biodiesel from B. unijugata and L. cylindrica was above 120°C while the phosphorus content was also below 1 ppm in both cases. The oxidative stability of B. unijugata was 44.30 ± 0.30 h, while that of L. cylindrica was lower than this value due to its high unsaturation. The copper strip corrosion value of the biodiesel was also found to be 1A. This study showed that the high free fatty acid content of B. unijugata and L. cylindrica seed oil can be reduced in a one-step pretreatment of esterification reaction using H2SO4 as catalyst thus reducing the problem of soap formation encountered when using oil with high free fatty acid for the production of biodiesel. 相似文献
16.
The fungal species ofRhizopus oryzae 2062 has the capacity to carry out a single stage fermentation process for lactic acid production from potato starch wastewater.
Starch hydrolysis, reducing sugar accumulation, biomass formation, and lactic acid production were affected with variations
in pH, temperature, and starch source and concentration. A growth condition with starch concentration approximately 20 g/L
at pH 6.0 and 30°C was favourable for starch fermentation, resulting in a lactic acid yield of 78.3%–85.5% associated with
1.5–2.0 g/L fungal biomass produced in 36 h of fermentation. 相似文献
17.
Churairat Moukamnerd Masahiro Kino-oka Minetaka Sugiyama Yoshinobu Kaneko Chuenchit Boonchird Satoshi Harashima Hideo Noda Kazuaki Ninomiya Suteaki Shioya Yoshio Katakura 《Applied microbiology and biotechnology》2010,88(1):87-94
To save cost and input energy for bioethanol production, a consolidated continuous solid-state fermentation system composed
of a rotating drum reactor, a humidifier, and a condenser was developed. Biomass, saccharifying enzymes, yeast, and a minimum
amount of water are introduced into the system. Ethanol produced by simultaneous saccharification and fermentation is continuously
recovered as vapor from the headspace of the reactor, while the humidifier compensates for the water loss. From raw corn starch
as a biomass model, 95 ± 3, 226 ± 9, 458 ± 26, and 509 ± 64 g l−1 of ethanol solutions were recovered continuously when the ethanol content in reactor was controlled at 10–20, 30–50, 50–70
and 75–85 g kg-mixture−1, respectively. The residue showed a lesser volume and higher solid content than that obtained by conventional liquid fermentation.
The cost and energy for intensive waste water treatment are decreased, and the continuous fermentation enabled the sustainability
of enzyme activity and yeast in the system. 相似文献
18.
Deficiencies in Cu, Se, and Zn impair one or more biochemical functions, and excess are associated with toxicity. Baseline
studies on the Ghanaian population are scanty. The study was undertaken to determine whether significant rural/urban differences
in the serum levels of Cu, Se, and Zn did exist. Forty males/60 females from rural and 50 males/50 females from urban Ghanaian
communities were sampled. Serum Cu, Se, and Zn were determined using flame atomic absorption spectrometry. Cu level for rural
and urban subjects was 997 ± 333 and 979 ± 290 μg/L, respectively (p = 0.68). However, Cu levels were significantly higher in the rural females (1,063 ± 367 μg/L) than the rural males (898 ± 249 μg/L;
p = 0.0085). Se levels for rural/urban subjects were 97 ± 36 and 87 ± 31 μg/L, respectively (p = 0.03). Zn levels in the rural/urban subjects were 312 ± 218 and 150 ± 102 μg/L, respectively (p = 0.002). Additionally, Zn was significantly higher in rural females (428 ± 204 μg/L) than the urban females (166 ± 103 μg/L;
p = 0.0002). Finally, Zn was significantly higher in rural females (428 ± 204 μg/L) than males (172 ± 116 μg/L; p = 0.0028). In conclusion, Cu, Se, and Zn were higher in the rural group compared to the urban group, and the generally low
Zn levels were confirmed in another cohort follow-up study. 相似文献
19.
Analysis of an exopolysaccharide of Sphingomonas paucimobilis GS-1 (EPS/GS-1) with respect to its rheological properties, cross-linking ability with chrome alum and performance test at
75 ± 5°C revealed its strong suspending ability, shear thinning property, and thixotrophic nature which are required to impart
desirable rheology to drilling mud. The organism fulfilled all the specified requirements and its properties were superior
to those of currently-used XC polymer (a xanthan product) for oil drilling applications. However, EPS/GS-1 was unstable in
the presence of bentonite at 100 ± 5°C during performance tests, in contrast to XC polymer.
Received 14 April 1999/ Accepted in revised form 26 July 1999 相似文献
20.
S. Guillouet J. H. Choi C. K. Rha A. J. Sinskey 《Applied microbiology and biotechnology》1999,51(2):235-240
Although many studies have examined the influence of culture conditions on the production and composition of polysaccharides,
little is known about the factors influencing the quality of exopolysaccharides (EPS). In this work we studied the effect
of yeast extract on the production, composition and molecular weight of the EPS zooglan produced by Zoogloea ramigera 115SLR. This bacterium was grown on a new completely defined synthetic medium and on a medium containing yeast extract. Growth
and polysaccharide production performances were comparable on the two media with a glucose to exopolysaccharide conversion
yield of 35% (g/g). The polysaccharides produced on these two media have an identical composition but a different molecular
weight and molecular weight distribution. The yeast extract medium leads to a more homogeneous polysaccharide solution.
Received: 12 June 1998 / Received revision: 19 September 1998 / Accepted: 11 October 1998 相似文献