Threatened fishes of the world: <Emphasis Type="Italic">Chalcalburnus tarichi</Emphasis> (Pallas 1811) (Cyprinidae) living in the highly alkaline Lake Van,Turkey

A member of the Cyprinidae family, the Chalcalburnus tarichi is a fish species that only inhabits the Lake Van Basin. The Lake Van represents an interesting ecosystem in the world, known as the biggest soda lake in the world, in that its water is highly alkaline with a pH of 9.8. C. tarichi has bright-silver color, its back is grayish green, and the abdominal region is silver. Its body is covered with small scales, and its eyes are large. It feeds on phyto and zooplanktons. Its average life span is 7 years, and the fish reaches reproductive maturity at 3 years old. C. tarichi is an diadrom fish that lives in the lake, but during the reproduction period it immigrates to the surrounding freshwater rivers returning after the reproduction period of April–July. In the past, pearl mullet was an attractive fresh fish for the local people and was easily caught during the spawning migration, resulting in over-fishing. The species was one of the highly endangered animals of Turkey before conservation studies, some 10 years ago, have started. At present, illegal fishing activities declined, although some locals are continuing to fish during the spawning season.     

Integration of acoustic telemetry and GIS to identify potential spawning areas for lake trout (Salvelinus namaycush)

Locations of potential spawning areas for lake trout (Salvelinus namaycush) were predicted in Lake Opeongo, Ontario, Canada using information gained via acoustic telemetry and geographic information system (GIS) technologies. From 1998 to 2000, 18 adult lake trout (mean fork length 553 mm) implanted with acoustic transmitters (battery life 2 years) were manually tracked. For evening fall locations within the erosive zone of the lake (determined using an existing sedimentation model), habitat variables (slope, depth, and effective fetch) were summarised using GIS. Sites selected by lake trout during the spawning window were in areas of mean fetch equal to 1.5 km and mean slope of 10.6% (n = 50 fixes). We used GIS to identify areas that matched the mean habitat criteria and thus locate potential spawning areas. This model correctly identified 19 of 21 known spawning sites, as well as additional sites used by spawning females in an earlier telemetry study. Depths of traditional fall netting sites are shallow compared to areas in which telemetered lake trout were found during evenings of the spawning period (means 3.1 vs. 5.1 m, respectively). Through the use of information on spawning habitat selection gained through telemetry and knowledge of the physical characteristics of the lake, we provide an alternative means of identifying potential spawning habitat for lake trout.     

Mercury accumulation in five species of freshwater fish in Lake Tyrifjorden,south-east Norway,with emphasis on their suitability as test organisms

Synopsis Mercury concentration in axial muscle of brown trout, Salmo trutta, whitefish, Coregonus lavaretus, Arctic charr, Salvelinus alpinus, smelt, Osmerus eperlanus and pike, Esox lucius, were studied in Lake Tyrifjorden during 1978–1982. Our data demonstrate that older and bigger fish on an average have higher mercury concentration than smaller and younger. Further, complex life histories as in brown trout influence the pattern of mercury accumulation. During young stages accumulation in brown trout is moderate, while accumulation in older stages is highly correlated to lake residency time. Based on our data we suggest the following requirements for a test organism and the collecting procedure; (1) life history should be simple with small sexual differences, (2) ageing should be easy and reliable, and (3) large representative samples should be easily obtained during (4) a fixed biological period i.e. the spawning period. We consider smelt as an appropriate test organism based on these criteria.     

Minimal gene flow from introduced brown trout (Salmo trutta L.) after 30 years of stocking

Lake Tinnsjø, Norway, has been heavily stocked over three decades with two different brown trout stocks. A population genetic survey based on 13 allozyme coding loci was conducted to investigate the genetic composition of the present trout population and the genetic impact of the two donors. Contrary to expectations, highly significant differences in allelic frequencies between Lake Tinnsjø trout and the two donor stocks were found at several loci, suggesting minimal gene flow from stocked trout to the wild populations. Pairwise genetic distance values were several times higher between donor stocks and Lake Tinnsjø samples than between the various spawning inlets to Lake Tinnsjø. F_st increased from 0.024 to 0.090, when samples from the donor stocks were included in the material. There were no deviations from expected Hardy–Weinberg distribution of genotypes in the spawning inlets. It was concluded that the donor stocks contributed little to the trout gene pool in the lake. A possible exception is the trout population below the lake outlet.     

Evaluating Stocking Efficacy in an Ecosystem Undergoing Oligotrophication

Oligotrophication has negatively affected fisheries production in many freshwater ecosystems and could conceivably reduce the efficacy of stockings used to enhance fisheries. In Lake Michigan, offshore oligotrophication has occurred since the 1970s, owing to reductions in total phosphorus (TP) inputs and nearshore sequestration of TP by nonindigenous dreissenid mussels. We evaluated simultaneous effects of stock enhancement and oligotrophication on salmonine species (Chinook salmon Oncorhynchus tshawytscha, lake trout Salvelinus namaycush, and steelhead O. mykiss) that support valuable recreational fisheries. We employed a novel application of an Ecopath with Ecosim model by conducting a full factorial simulation experiment. Our design included multiple levels of salmonine stocking, consumption by invasive quagga mussels (Dreissena bugensis), and TP that were informed by manager interests. Under all levels of TP and quagga mussel consumption, our results showed that stock enhancement could still increase salmonine biomass, but positive responses were stronger for lake trout and steelhead than Chinook salmon. Simulations showed that quagga mussel consumption has deleterious effects on pelagic-oriented prey fishes and Chinook salmon, which feed almost exclusively on the pelagic-oriented alewife (Alosa pseudoharengus). In summary, results from our simulation experiment suggested that lake trout and steelhead are better suited to the current ecosystem than Chinook salmon, and therefore, stock enhancement provides the highest gains for these two species. Furthermore, simulated biomass of all recreational salmonine species increased with increasing TP, indicating the need for managers to consider how potential future oligotrophication will limit the carrying capacity of salmonine biomass in Lake Michigan.     

Temporal constraints on the potential role of fry odors as cues of past reproductive success for spawning lake trout

Deciding where to reproduce is a major challenge for most animals. Many select habitats based upon cues of successful reproduction by conspecifics, such as the presence of offspring from past reproductive events. For example, some fishes select spawning habitat following odors released by juveniles whose rearing habitat overlaps with spawning habitat. However, juveniles may emigrate before adults begin to search for spawning habitat; hence, the efficacy of juvenile cues could be constrained by degradation or dissipation rates. In lake trout (Salvelinus namaycush), odors deposited by the previous year's offspring have been hypothesized to guide adults to spawning reefs. However, in most extant populations, lake trout fry emigrate from spawning reefs during the spring and adults spawn during the fall. Therefore, we postulated that the role of fry odors in guiding habitat selection might be constrained by the time between fry emigration and adult spawning. Time course chemical, physiological, and behavioral assays indicated that the odors deposited by fry likely degrade or dissipate before adults select spawning habitats. Furthermore, fry feces did not attract wild lake trout to constructed spawning reefs in Lake Huron. Taken together, our results indicate fry odors are unlikely to act as cues for lake trout searching for spawning reefs in populations whose juveniles emigrate before the spawning season, and underscore the importance of environmental constraints on social cues.     

Use of cover habitat by bull trout, <Emphasis Type="Italic">Salvelinus confluentus</Emphasis>, and lake trout, <Emphasis Type="Italic">Salvelinus namaycush</Emphasis>, in a laboratory environment

Lacustrine-adfluvial bull trout, Salvelinus confluentus, migrate from spawning and rearing streams to lacustrine environments as early as age 0. Within lacustrine environments, cover habitat provides refuge from potential predators and is a resource that is competed for if limiting. Competitive interactions between bull trout and other species could result in bull trout being displaced from cover habitat, and bull trout may lack evolutionary adaptations to compete with introduced species, such as lake trout, Salvelinus namaycush. A laboratory experiment was performed to examine habitat use and interactions for cover by juvenile (i.e., <80 mm total length) bull trout and lake trout. Differences were observed between bull trout and lake trout in the proportion of time using cover (F _1,22.6 = 20.08, P < 0.001) and bottom (F _1,23.7 = 37.01, P < 0.001) habitat, with bull trout using cover and bottom habitats more than lake trout. Habitat selection ratios indicated that bull trout avoided water column habitat in the presence of lake trout and that lake trout avoided bottom habitat. Intraspecific and interspecific agonistic interactions were infrequent, but approximately 10 times greater for intraspecific interactions between lake trout. Results from this study provide little evidence that juvenile bull trout and lake trout compete for cover, and that species-specific differences in habitat use and selection likely result in habitat partitioning between these species.     

Spawning behaviour of taimen (<Emphasis Type="Italic">Hucho taimen</Emphasis>) from the Uur River,Northern Mongolia

This study presents the first observations of Hucho taimen spawning in the wild based on underwater video recordings. One pair of taimen was monitored during a 19 h period, supplemented with visual observations from two other spawning pairs. We recorded two full spawning events performed in two different locations separated by approximately 30 m. The absence of an established male hierarchy along a nesting female was the most important difference between taimen and other salmonine breeding biology. Taimen spawning, based on our observations, is a single pair event. The male prevented the approach of other males by launching intense attacks that extended for several meters away from the redd. Our data suggests that taimen females, differently from other salmonines, do not cover their eggs immediately after having spawned but rest for a variable number of minutes before covering them.     

Salmonid predator–prey dynamics in Lake Pend Oreille,Idaho, USA

Our objective was to evaluate the long-term sustainability of lake trout Salvelinus namaycush and rainbow trout Oncorhynchus mykiss populations subjected to a range of fishing mortality (F) in Lake Pend Oreille, Idaho, USA, while providing for bull trout Salvelinus confluentus and kokanee Oncorhynchus nerka recovery. In order to achieve our objective, we developed a density-dependent stochastic predator–prey simulation model for the three major predators (lake trout, rainbow trout, and bull trout) on kokanee in Lake Pend Oreille. As F increased from 0.0 to 1.0, lake trout numbers in 2015 declined 90% for gillnetting, 76% for angling, and 48% for trap netting. At fishing mortality rates observed in Lake Pend Oreille during 2006, all methods combined and angling alone suppressed the lake trout population, but not gillnetting or trap netting alone. As F increased from 0.0 to 0.3, rainbow trout numbers in 2015 declined by 38%. Abundance of adult bull trout increased by 5.8% per year during 1996–2006, after implementation of no-kill regulations, which met the Federal Recovery Plan criterion of a stable or increasing trend in abundance. By 2010, total consumption of kokanee by lake trout, rainbow trout, and bull trout would increase by 20% if fishing mortality on lake trout and rainbow trout declined by 30% from 1996 levels, and would decrease by 14% if fishing mortality on lake trout and rainbow trout increased by 30% from 1996 levels. At rates of fishing mortality exerted on lake trout and rainbow trout in 2006, the likelihood of kokanee collapse was 65% within the next decade. Therefore, fishing mortality would need to be at least 6% higher on both lake trout and rainbow trout to reduce the likelihood of kokanee collapse to 50%. We conclude that kokanee biomass is presently out of balance with predation in Lake Pend Oreille, because kokanee production cannot compensate for all predation loss. Our findings suggest that a combination of unusually high kokanee production and unusually low predation are likely needed for kokanee to survive the next decade in Lake Pend Oreille.     

Effects of a Whole-lake,Experimental Fertilization on Lake Trout in a Small Oligotrophic Arctic Lake

We tested whether increased phosphorus and nitrogen concentrations would affect a lake trout (Salvelinus namaycush) population in a small oligotrophic lake with a benthically dominated food web. From 1990 to 1994, nitrogen and phosphorus were added to Lake N1 (4.4 ha) at the arctic Long-Term Ecological Research site in Alaska. We used mark/recapture methods to determine the lake trout population size, size structure, recruitment, and individual growth from 1987 to 1999. Data were also collected on water chemistry and food availability. Fertilization resulted in increased pelagic primary productivity, chlorophyll a, turbidity, snail density, and hypoxia in summer and winter. Lake trout density was not affected by the manipulation however growth and average size increased. Recruitment was high initially, but declined throughout the fertilization. These results suggest that lake trout were affected through increased food availability and changes to the physical characteristics of the lake. During fertilization, hypoxia near the sediments may have killed over-wintering embryos and decreased habitat availability. Although lake trout responded strongly to increased nutrients, loss of recruitment might jeopardize lake trout persistence if arctic lakes undergo eutrophication.     

Field activity of lake trout during the reproductive period monitored by electromyogram radiotelemetry

Many lake trout Salvelinus namaycush spawn in shallow areas along windswept shores. However, precise determination of time and location of spawning is limited by its nocturnal occurrence, and possibly by some postulated, but unproven, spawning in deep water by certain populations. In White Pine Lake, Ontario, a well-studied experimental lake, lake trout spawn in two shallow locations where some direct observation is possible. Radiotelemetry apparatus was used in 1991 to obtain records of the electromyograms (EMGs) produced by muscle activity in one adult male and one adult female lake trout, captured from the lake before their reproductive period and returned to the lake at the beginning of the spawning period. Both fish survived, though only the male was detected as active over the main spawning site, where it showed evening EMG activity patterns indicating considerable activity. At other times of day, when not located over the spawning site, but present elsewhere in the lake, the male's EMG (i.e. muscle) values were considerably reduced. At the cessation of the spawning period, the transmitter-equipped male's EMG record showed no further pattern indicative of high activity during what had formerly been the daily spawning period. The female was tracked as she moved around the lake, but based on both her location and level of EMG activity, is thought not to have spawned. In 1992, a second male was captured, equipped with a transmitter and released again. This fish failed to show spawning activity but, as with the 1991 female, moved fairly considerable distances in the lake. In one instance, in a swim of short duration (6 min), well away from the spawning grounds, this fish displayed EMG activity levels resembling those of the high activity of spawning. The results obtained appear to demonstrate that EMG telemetry could be used to assess the level of muscular activity occurring in lake trout, especially in relation to reproductive behaviour, and when they cannot be directly observed.     

Spatial and Temporal Relations between Fluvial and Allacustrine Yellowstone Cutthroat Trout, Oncorhynchus clarki bouvieri, Spawning in the Yellowstone River, Outlet Stream of Yellowstone Lake

Yellowstone cutthroat trout (YCT), Oncorhynchus clarki bouvieri, that spawn in the outlet of Yellowstone Lake show two potamodromous migration patterns, fluvial and allacustrine. The main purpose of this study was to determine whether those fluvial and allacustrine YCT represent reproductively isolated stocks. Redd surveys indicated spawning occurred during about 5 consecutive weeks between late May and mid-July 1993–1995. Lake fish (N=6), defined as radiotagged YCT that entered Yellowstone Lake after the spawning period (i.e. allacustrine pattern), were found in the river between the lake outlet (river kilometer [Rkm] 0) and Rkm 20.0 during spawning. Probable lake fish (N=28; tagged YCT that were last detected near the lake outlet) were found between Rkm 0 and Rkm 22.5 during spawning. River fish (N=4; tagged YCT that remained in the river when annual tracking concluded in fall, i.e. fluvial pattern) were found between Rkm 1.1 and Rkm 18.0 during spawning. Fidelity to spawning areas used between consecutive years was suggested by one of five lake fish and the single river fish for which data were available. Spatial overlap in spawning and a lack of temporal separation between the life-history types during spawning suggested that fluvial and allacustrine YCT were not reproductively isolated. Radiotagging, as well as visual observations made annually from boats during April and May, indicated fluvial YCT overwintered downstream from Rkm 14 and were few, probably on the order of 10% of all YCT that spawned in the Yellowstone River.     

Comparison of brook trout reproductive success and recruitment in an acidic adirondack lake following whole lake liming and watershed liming

Limestone applications to the catchment of one tributary to Woods Lake were highly effective in reducing stream acidity and stabilizing seasonal fluctuations in pH. The resulting improvement in stream water quality also led to a dramatic shift in reproductive strategy of the Woods Lake brook trout population. Prior to catchment liming, brook trout in Woods Lake were restricted to spawning on poor quality near shore substrate with limited ground water seepage. Reproductive success was limited by high mortality of eggs and larvae and recruitment from in lake spawning was not successful. Spawning brook trout did not utilize the tributary for spawning prior to watershed liming. Mitigation of acidity in the tributary, by catchment liming, effectively extended the spawning habitat available to the Woods Lake brook trout population and one year following treatment brook trout spawned successfully in the tributary for the first time in 6 years of observation. Significant recruitment of young trout into the lake population occurred from 1991 through 1993, although the absolute number of fish captured was relatively small. In the fall of 1993, four year classes of naturally spawned brook trout were present in the lake. Although reproductive success was enhanced by improving tributary spawning habitat in the Woods Lake basin, self maintenance of the population may be limited by low recruitment rates of young trout, due to high levels of summer mortality resulting from predation. Mitigation of this constraint would require substantially higher levels of fry production than were observed in Woods Lake and/or enhanced refugia for young trout. The results of this experiment suggest that re-establishment of tributary spawning populations of brook trout may be possible, with future reductions in acidic deposition, in acidic Adirondack lakes with limited in-lake spawning habitat.     

Elevated summer temperatures delay spawning and reduce redd construction for resident brook trout (Salvelinus fontinalis)

Redd (nest) surveys for resident brook trout (Salvelinus fontinalis) were conducted annually in a mountain lake in northern New York for 11 years with multiple surveys conducted during the spawning season in eight of those years. Repeated surveys throughout the spawning season allowed us to fit an individually based parametric model and estimate the day of year on which spawning was initiated, reached its midpoint, and ended during each year. Spawning phenology was then assessed relative to (1) mean of maximum daily air temperature and (2) mean of maximum daily water temperature at the lake bottom during summer in each year using a linear model. Elevated temperatures in summer were correlated with a delay in spawning and a reduction in the total number of redds constructed. Increasing the summer mean of maximum daily air temperatures by 1 °C delayed spawning by approximately 1 week and decreased the total number of redds constructed by nearly 65. Lake spawning brook trout select redd sites based on the presence of discharging groundwater that is relatively constant in temperature within and across years, leading to relatively consistent egg incubation times. Therefore, delayed spawning is likely to delay fry emergence, which could influence emergence synchrony with prey items. This work highlights non‐lethal and sub‐lethal effects of elevated summer temperatures on native resident salmonids in aquatic environments with limited thermal refugia.     

Cost of predation avoidance in young-of-year lake trout (Salvelinus namaycush): growth differential in sub-optimal habitats

Selection of habitat to avoid predation may affect the diet of young-of-year (YOY) lake trout (Salvelinus namaycush). YOY lake trout may use inshore habitat to avoid predation; this habitat may be sub-optimal for growth. To test this, YOY lake trout were penned in nearshore and offshore pelagic areas of two arctic lakes. Toolik Lake had a lake trout population, the other lake, S6, did not. YOY lake trout in Toolik Lake lost weight, but those offshore lost less weight. The YOY lake trout in Lake S6 gained weight and those offshore gained more weight. The primary diet item of the YOY lake trout in both lakes during this experiment was the zooplankter Diaptomis probilofensis; it was also one of the most abundant species. However, its density inshore in Lake S6 was similar to inshore and offshore densities in Toolik Lake. The increased availability of alternative zooplankton prey in Lake S6 may account for the growth differential of YOY lake trout in Lake S6 relative to Toolik Lake. Bioenergetic modeling of YOY lake trout suggests that growth similar to that in the offshore of Lake S6 would be necessary for successful recruitment. If the reduced zooplankton availability in Toolik Lake leads to the reduced growth of YOY in the inshore and offshore pelagic areas, then these fish will be more susceptable to winter predation/starvation. For YOY lake trout to survive in Toolik Lake they most likely shift to feeding on benthic prey before the end of their first summer. Dept. of Chemical Engineering     

Variability of kokanee and rainbow trout food habits,distribution, and population dynamics,in an ultraoligotrophic lake with no manipulative management

Crater Lake is a unique environment to evaluate the ecology of introduced kokanee and rainbow trout because of its otherwise pristine state, low productivity, absence of manipulative management, and lack of lotic systems for fish spawning. Between 1986 and 2004, kokanee displayed a great deal of variation in population demographics with a pattern that reoccurred in about 10 years. We believe that the reoccurring pattern resulted from density dependent growth, and associated changes in reproduction and abundance, driven by prey resource limitation that resulted from low lake productivity exacerbated by prey consumption when kokanee were abundant. Kokanee fed primarily on small-bodied prey from the mid-water column; whereas rainbow trout fed on large-bodied prey from the benthos and lake surface. Cladoceran zooplankton abundance may be regulated by kokanee. And kokanee growth and reproductive success may be influenced by the availability of Daphnia pulicaria, which was absent in zooplankton samples collected annually from 1990 to 1995, and after 1999. Distribution and diel migration of kokanee varied over the duration of the study and appeared to be most closely associated with prey availability, maximization of bioenergetic efficiency, and fish density. Rainbow trout were less abundant than were kokanee and exhibited less variation in population demographics, distribution, and food habits. There is some evidence that the population dynamics of rainbow trout were in-part related to the availability of kokanee as prey.     

Effects of an Introduced Piscivore on Native Trout: Insights from a Demographic Model

Introductions of exotic species pose a significant threat to the persistence of many native populations, including many inland fishes. In 1994, piscivorous lake trout (Salvelinus namaycush) were discovered in Yellowstone Lake, Yellowstone National Park, Wyoming, USA, one of the last strongholds of the native Yellowstone cutthroat trout (Oncorhynchus clarki bouvieri). Predation by lake trout is expected to lead to a substantial decline in the native cutthroat trout population, which may have significant negative consequences for terrestrial predators that depend on cutthroat trout for prey and for the recreational fishery of the Park. We developed a matrix demographic model for the cutthroat trout population in Yellowstone Lake to identify the life stages that are most critical for understanding population dynamics. Parameter estimates (vital rates) were manipulated to explore the possible consequences of lake trout invasion. Comparisons of our results with current estimates of population trend and age structure suggested that our model reflected current conditions of the system. Elasticity analysis of the model revealed that population growth was most sensitive to annual survival of young trout, the group that is expected to be most vulnerable to lake trout predation. Projection of our deterministic model suggested that, in addition to a decline in abundance of cutthroat trout, the effects of lake trout may be manifest as changes in age and breeding structure of the population. Simulations of a stochastic version of the model indicated that a 60% or greater decline in the cutthroat trout population could be expected within 100 years if the lake trout population were permitted to grow uncontrolled. However, an effective control strategy that prevented the establishment of a large population of lake trout substantially reduced population decline, although the reduction in the availability of adult trout to terrestrial predators and anglers may be still be substantial (20–40%). In addition to current control activities in place in the Park, we recommend a renewed emphasis on understanding and monitoring juvenile life stages of cutthroat trout. Our results demonstrate the value of existing data sets for developing models to estimate the potential impact of biological invasions on the management and conservation of native populations, especially when opportunities and resources for additional empirical studies are limited.     

Effectiveness of lake trout (Salvelinus namaycush) suppression in Lake Pend Oreille,Idaho: 2006–2016

The nonnative lake trout (Salvelinus namaycush Walbaum, 1792) population in Lake Pend Oreille, Idaho increased exponentially during 1999–2006. This led to an unsustainable level of predation mortality on kokanee (Oncorhynchus nerka Walbaum, 1792), increased the conservation threat to native bull trout (Salvelinus confluentus Suckley, 1859), and jeopardized the popular recreational fishery for kokanee and rainbow trout (Oncorhynchus mykiss Walbaum, 1792). In response, lake trout were suppressed since 2006 using incentivized angling, gill netting, and trap netting. From 2006 through 2016, 193,982 lake trout were removed (50% by gill netting; 44% by angling; 6% by trap netting). During this period, age-8 + (adult) lake trout abundance declined by 64%, age-3 (recruit) abundance declined by 56%, and mean total annual mortality (A) was 31.1%. Lake trout did not show evidence of a density-dependent response. Kokanee did not collapse and rebounded to abundances not observed since before lake trout expansion. Bull trout abundance declined during suppression, but the population was sustained. Lake trout suppression allowed a harvest fishery for kokanee and trophy fishery for rainbow trout to be restored. We conclude that suppression can be an effective management action for mitigating effects of nonnative lake trout in a large, deep lake.

     

Long-term captive breeding does not necessarily prevent reestablishment: lessons learned from Eagle Lake rainbow trout

Captive breeding of animals is often cited as an important tool in conservation, especially for fishes, but there are few reports of long-term (<50 years) success of captive breeding programs, even in salmonid fishes. Here we describe the captive breeding program for Eagle Lake rainbow trout, Oncorhynchus mykiss aquilarum, which is endemic to the Eagle Lake watershed of northeastern California. The population in Eagle Lake has been dependent on captive breeding for more than 60 years and supports a trophy fishery in the lake. Nevertheless, the basic life history, ecological, and genetic traits of the subspecies still seem to be mostly intact. Although management has apparently minimized negative effects of hatchery rearing, reestablishing a wild population would ensure maintenance of its distinctive life history and its value for future use as a hatchery fish. An important factor that makes reestablishment possible is that the habitat in Eagle Lake is still intact and that Pine Creek, its major spawning stream, is recovering as habitat. With the exception of an abundant alien brook trout (Salvelinus fontinalis) population in Pine Creek, the habitat factors that led to the presumed near-extinction of Eagle Lake rainbow trout in the early twentieth century have been ameliorated, although the final stages of reestablishment (eradication of brook trout, unequivocal demonstration of successful spawning migration) have still not been completed. The Eagle Lake rainbow trout story shows that long-term captive breeding of migratory salmonid fishes does not necessarily prevent reestablishment of wild populations, provided effort is made to counter the effects of hatchery selection and that natural habitats are restored for reintroduction. Long-term success, however, ultimately depends upon eliminating hatchery influences on wild-spawning populations. Extinction of Eagle Lake rainbow trout as a wild species becomes increasingly likely if we fail to act boldly to protect it and the Eagle Lake watershed.