水团花黄酮类成分及其体外抗病毒活性

从水团花乙醇提物的乙酸乙酯部位分离到6个黄酮类化合物,根据光谱数据分别鉴定为柚皮素（1）、圣草酚（2）、槲皮素（3）、柚皮素-7-O-β-D-葡萄糖苷（4）、圣草酚-7-O-β-D-葡萄糖苷（5）、槲皮素-3-O-β-D-葡萄糖苷（6）,化合物1、2,4和5为首次从该植物中分离得到。采用细胞病变抑制法（CPEr eductionassay）和MTT法测定化合物的体外抗病毒活性,结果显示,三个黄酮苷元1、2和3均具有不同程度的体外抑制呼吸道合胞病毒（RSV）和柯萨奇B3型病毒（CVB3）活性,反之,3个黄酮苷均不显示活性。     

不同化学杂交剂(CHA)对小麦花药同工酶影响的研究

在小麦叶枕距±2cm时,喷施4种不同化学杂交剂（CHA）后,分别取小孢子处于不同发育阶段的花药,进行过氧化酶（POD）、淀粉酶（Amy）和酯酶（Est）等同工酶的分析。研究表明：在小孢子母细胞减数分裂期,处理2、3和4三种CHA对POD的A1、A2;B2、B4和B5,C1、C2和C3同工酶带的活性均有明显的抑制作用;处理5除去对A1和A2表现抑制外,对其他酶带的活性均有增强作用。在单枚早期,处理2和3的A、B和C区POD同工酶活性均明显低于对照;处理4和5上述各区POD同工酶活性却明显高于对照。在上述两个发育时期,处理2对Amy1区酶活性有增强作用,而处理3、4和5对该区酶活性却表现了专一性的抑制。各处理对Est同工酶A区和B区的酶活性主要表现为抑制。实验结果表明,不同的CHA均通过干扰花药的物质和能量代谢而导致雄性生理性不育。     

相似蜂海绵相关真菌杂色曲霉F62活性代谢产物研究

研究了1株相似蜂海绵相关真菌F62的活性代谢产物,经分子系统学分析表明该真菌属于杂色曲霉。将F62菌株用大米固体培养基在室温（约25℃）下静置培养45d,经乙酸乙酯超声萃取得到粗提物。通过硅胶柱层析、Sephadex LH-20凝胶柱层析和HPLC等色谱手段,分离得到了6个化合物。通过核磁共振、质谱等波谱分析手段可鉴定出其结构分别为Alantrypinone（1）、洛伐他汀（2）、甲酯型莫纳克林K（3）、土曲霉酮（4）、土震素B（5）和麦角甾醇（6）。化合物1系首次从该属真菌中分离得到,化合物2－5为首次从该种真菌中分离得到。首次对化合物3的碳谱数据进行报道。初步的药理研究表明,化合物4具有体外抗炎活性。     

EB病毒潜伏膜蛋白1通过结合TRAFs调控NF-κB

为了探讨EB病毒潜伏膜蛋白1（LMP1）的致瘤机制,对鼻咽癌中LMP1激活重要的核转录因子NF－κB机制进行了研究,首先,采用免疫共沉淀－蛋白质印迹在稳定表达LMP1的鼻咽癌细胞系HNE2－LMP1中证实LMP1与TRAF1,2,3结合形成免疫共沉淀复合物,进一步以野生型LMP1及其三种突变体的鼻咽癌细胞系LMP1（野生型, wt),HNE2－LMP1 del187-351(CTAR1缺失型）,HNE2－LMP1（1－231）,（CTAR2缺失型）,HNE2－LMP1（1－187）（羰基端胞浆区缺失型）,HNE2－pSG5(空白载体型）为材料,结合NF－κB报道基因质粒（pG12-NF-B-luc)的荧光素酶活性表达分析NF－κB的活性,证实：较之母细胞,野生型LMP1活化NF－B达13．8倍,LMP1（1－187）几乎不活化NF－kb,LMP1(1-231)活化NF－kB 达4．9倍,LMP1（del187-351)活化NFκB达9．1倍,TRAF1过表达升高LMP1（ wt)及LMP1（1－231）介导的NF－κB活性,而对LMP1（del187-351)活化NFκB无影响,TRAF3过表达或TRAF3负显性突变体抑,制LMP1（wt)及LMP1（1－231）介导的NF－κB活性而不影响LMP1（del187－351）活化NF－κB,TRAF2过表达升高LMP1（wt),LMP1(1-231)及LMP1（del 187-351)介导的NF－kB活性,这些结果表明：鼻咽癌中LMP1通过TRAF1,TRAF2或TRAF3调控NF－kB,TRAF1和TRAF3主要通过CTAR1发挥作用,TRAF2的作用主要是通过CTAR1和CTAR2介导的。     

南海疏枝刺柳珊瑚中甾醇类化合物的研究

本文对采自海南三亚海域的疏枝刺柳珊瑚(Echinogorgia pseudossapo)化学成分进行研究,分离到11个甾醇类化合物。经波谱数据分析,分别鉴定为cholest-5-en-3β-ol(1),24-methylene-cholest-4-ene-3β,6β-diol(2),24-norcholesta-22-en-3β-ol(3),acanthovagasteroid A(4),calicoferol E(5),calicoferol F(6),6-hydroxy-cholest-4-ene-3-one(7),echinoflorasterol(8),echissaposterol(9),24-methylcholest-5-en-3β,7α-diol(10)和24-methylcholest-5,22(E)-dien-3β,7α-diol(11)。除化合物8外,其余化合物均首次从该种海洋动物中分离得到。     

川西北高原不同海拔高度披碱草与垂穗披碱草核型分析

目的：为研究不同海拔居群染色体核型变异及进化程度,为披碱草的分类及进化研究提供细胞学资料。方法：以川西北高原6个海拔梯度的披碱草居群和5个海拔梯度的垂穗披碱草居群为材料,采用滴片法制片对染色体进行核型分析。结果：披碱草居群D3057（海拔3 057 m）、D2682（海拔2 682 m）的核型公式为2n=6x=42=42m,为1B型;D2384（海拔2 384 m）核型公式为2n=6x=42=42m(4sat),为1A型;D2143（海拔2 143 m）、D1747（海拔1 747 m）的核型公式为2n=6x=42=40m+2sm,为1B型;D1589（海拔1 589 m）的核型公式为2n=6x=42=36m+6sm,为2B型。垂穗披碱草Y2126（海拔4 050 m）、Y2117（海拔3 800 m）、Y2291a(4 150 m)居群的核型公式为2n=6x=42m,ZY3034（海拔3 200 m）的居群核型公式为2n=6x=40m+2M,Y2122（海拔4 800 m）核型公式为2n=6x=40m+2sm,5个居群的核型类型均为1B型。基于核型对称性与进化指数,披碱草居群D158...     

长穗桑中的Diels-Alder型加合物

从长穗桑的茎皮中首次分离到9个Diels—Alder型加合物,通过NMR、MS等波谱分析手段分别鉴定为mulberrofuran K（1）,mulberrofuran G（2）,guangsangon L（3）,kuwanon J（4）,kuwanonx（5）,guangsangonG（6）,guangsangon B（7）,guangsangon D（8）,kuwanon P（9）。化合物1—9进行了抗氧化活性筛选。结果表明,在10^-5M浓度下,化合物1,2,5—7,9对Fe^2＋-半胱氨酸诱导的肝微粒体脂质过氧化产生的丙二醛（malondialdehyde,MDA）有抑制作用（抑制率大于50％）。     

植物内生真菌F4a次级代谢产物的分离鉴定以及降血糖、抗氧化活性的研究

研究植物内生真菌布雷青霉菌（Penicillium brefeldianum） F4a次级代谢产物的提取分离方法、结构鉴定及其降血糖和抗氧化活性。采用液体发酵培养,大孔吸附树脂HP20提取后,经硅胶柱色谱、Sephadex LH-20凝胶柱色谱、ODS反相开放柱色谱和高效液相色谱等手段进行分离,应用核磁共振等技术进行结构鉴定;采用紫外分光光度吸收法进行降血糖和抗氧化活性筛选。结果表明,分离得到6个化合物,分别鉴定为环（L-色氨酸-L-脯氨酸）（1）、3,3′-Methylenebis（4-hydroxy-6-methyl-2H-pyran-2-one）（2）、2-（2′S-Hydroxypropyl）-5-methyl-7-hydroxychromone（3）、染料木素（4）、大豆素（5）和苯酚（6）。化合物1和2具有一定的抗氧化活性,化合物4和5具有较强的抗氧化活性和降血糖活性。化合物2和3为首次从青霉属真菌中分离得到。化合物1和2的ABTS自由基清除活性为首次报道。     

一个短指症A型合并遗传性肾病综合征家系

1951年Bell将遗传性短指症(brachydact, ylia）分为5型（A, B, C, D, E）及若干亚 型,短指症A型主要是中指骨变短。Bell又把 A型分为A-1, A-2和A-3型。1975年 McKusick增加了A-4和A-5型。现在短指症 A型共分为5个亚型。     

拟康氏木霉菌YIM PH30002铁载体活性化学成分北大核心CSCD

通过双层琼脂平板法对拟康氏木霉菌YIM PH30002在三种培养基条件下产铁载体能进行了半定量分析检测,该菌株在PDB培养基中,产铁载体成分的量最多。利用多种色谱技术从菌株YIM PH30002的PDB发酵液的乙酸乙酯提取物中共获得了5个聚酮类代谢产物,经波谱数据分析与文献比对,化合物分别鉴定为：konginginin A（1）、konginginin B（2）、konginginin D（3）、konginginin F（4）、konginginin M（5）。化合物1~4表现有铁载体活性,其中化合物2的铁载体活性相对较强,MTC值约为300μg/m L。但化合物均无显著抗菌生物活性。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.