丝状真菌SC—2产β—胡萝卜素的研究

利用改良的ＳＭＡ培养基富集培养从植物的落花上分离筛选出的产β－胡萝卜素的菌株ＳＣ－２。该菌株在以棉籽饼粉为主要原料的培养基上生长良好。最高生物量达５４ｍｇ干菌体／ｍｌ培养液。胡萝卜素含量１．８５ｍｇ／ｇ干菌体。ＨＰＬＣ测定β－胡萝卜素占总胡萝卜素的９０．５％。     

丝状真菌SC－2产β－胡萝卜素的研究

利用改良的ＳＭＡ培养基富集培养从植物的落花上分离筛选出的产β─胡萝卜素的菌株ＳＣ—２。该菌株在以棉籽饼粉为主要原料的培养基上生长良好。最高生物量达５４ｍｇ干菌体／ｍｌ培养液。胡萝卜素含量１．８５ｍｇ／ｇ干菌体。ＨＰＬＣ测定β─胡萝卜素占总胡萝卜素的９０．５％。     

丝状真菌SC－2产β－胡萝卜素的研究

利用改良的ＳＭＡ培养基富集培养从植物的落花上分离筛选出的产β─胡萝卜素的菌株ＳＣ—２。该菌株在以棉籽饼粉为主要原料的培养基上生长良好。最高生物量达５４ｍｇ干菌体／ｍｌ培养液。胡萝卜素含量１．８５ｍｇ／ｇ干菌体。ＨＰＬＣ测定β─胡萝卜素占总胡萝卜素的９０．５％。     

微生物生产弹性蛋白酶的研究

从１０个不同省区采集的５００多个土样中,分离得到了多株产弹性蛋白酶的菌株,主要集中在黄杆菌属和色杆菌属中。经过细胞工程技术处理后,黄杆菌属中的Ｆ－１２２菌在摇瓶发酵中的产酶能力达到２４０ｕ／ｍｌ,比出发菌株提高了１．５倍。该酶在Ｔｒｉｓ—ＨＣｌ缓冲液中最适ｐＨ为８．０,最适酶解温度４３℃。在ｐＨ６．０—９．５范围内稳定。ＣｕＳＯ４、ＺｎＳＯ４和ＦｅＳＯ４强烈抑制酶的活性,ＮａＣｌ、ＫＣｌ对酶活影响微弱。６０℃,３０ｍｉｎ酶活完全丧失。     

双抗体夹心ELISA检测人C蛋白的研究

本文建立了检测人Ｃ蛋白的双抗夹心ＥＬＩＳＡ方法。该方法的最佳实验条件为：－抗浓度５－８．５μｇ／ｍｌ;二抗密度为１：２００－１：１０００倍稀释。制作了Ｃ蛋白浓度与ＯＤ值的关系的工作曲线,指出了用内插法测定Ｃ蛋白的最佳浓度范围为０－１００ｍｇ／ｍｌ之间。     

茶儿茶素氧化产物体外清除·OH自由基作用的研究

本文采用２－脱氧－Ｄ－核糖（ＤＲ）法产生．ＯＨ,测定了不同浓度的茶儿素氧化产物Ａ及Ｃ对．ＯＨ的清除作用。结果表明,在一定的浓度范围内,氧化产物Ａ及Ｃ均有很强的清除．ＯＨ的作用且最佳清除浓度为２００μｇ／ｍｌ。在茶多酚、氧化产物Ａ及Ｃ三种物质中,以产物Ｃ的清除作用最强,其ＩＣ５０值为７．３μｇ／ｍｌ,其次是Ａ,ＩＣ５０为１０．１μｇ／ｍｌ,最后是茶多酚,ＩＣ５０值为７０μｇ／ｍｌ。     

血啉甲醚体外光敏效应观察

本文用化学发光法检测ＨＭＭＥ的’Ｏ２和ＲＯＳ产量,并与ＨｐＤ相比较,同时观察ＨＭＭＥ光敏效应与浓度、激光照射功率密度、照射时间、激光波长的关系。结果显示：ＨＭＭＥ浓度在２．５－４０μｇ／ｍｌ范围内,其光敏效应随浓度的增加而呈线形上升,以后便略下降;５７８．２ｎｍ激光以１０ｍＷ／ｃｍ＾２照射２．５μｇ／ｍｌ,ＨＭＭＥ的’Ｏ２和ＲＯＳ产量是ＨｐＤ的８倍;ＨＭＭＥ光敏效应与激光照射的功率密度密切相关。各     

天竺葵花清除自由基作用的研究

用化学发光法研究了天竺葵花粗担液对超氧阴离子自由基（Ｏ２）＾＝和羟自由基（ＯＨ）的清除作用,用分光不镀法研究了它对１,１－二苯基－２－苦肼基自由基（ＤＰＰＨ０的清除作用。结果表明,天竺葵花具有很强的清除自由基活性。其粗提物清除Ｏ２＾－分别为３．５μｇ／ｍｌ（红花）,３．０μｇ／ｍｌ（粉花）,１．６μｇ／ｍｌ（浅粉花）,与茶多酚（ＩＣ５０＝１．１μｇ／ｍｌ）相近,清除ＯＨ的ＩＣ５０分别为５．７μｇ／     

高密度发酵生产突变型重组人肿瘤坏死因子rhTNFα-DK2的研究

通过对发酵基质和发酵关键参数的优化,确定了发酵培养基的磷酸盐浓度为０．１５Ｍ,甘油浓度为１．２ｍｌ／Ｌ,补料中甘油浓度为２０ｍｌ／Ｌ,发酵过程中溶解氧控制在３０％～６０％,ｐＨ控制在６．８５左右。在５Ｌ在ＮＢＳ－Ｂｉｏｆｌｏ３０００型自动控制发酵罐中采取加速补料的补料分批培养,重组大肠杆菌ＹＫ５３７／ｐＳＢ－ＴＫ经１０ｈ３０°Ｃ培养和５ｈ４２°Ｃ诱导培养,最终密度达到６０ＯＤ６００,ｒｈＴＮＦα－ＤＫ２的表达量占菌体总蛋白的５０％以上,每升发酵液纯化可得到近２ｇ的ｒｈＴＮＦα－ＤＫ２。     

碱性淀粉酶产生菌的筛选和产酶条件的研究

对采自全国各地的５０份上样进行筛选,获得一株产酶活性较高的菌株Ｂ－９５４５,通过摇瓶培养确定了产酶的最适培养基Ⅱ（ｇ／１００ｍｌ）：复合蛋白胨１．０;玉米淀粉１．５;玉米浆１．５;ＮａＣ１０．５;Ｋ２ＨｐＯ４０．１;ＭｇＳＯ４０．０２;ＣａＣＯ３０．５;Ｔｗｅｅｎ８００．１;ｐＨｇ—１０,在该条件下,３０℃荡培养４８ｈ,酶活力可达５９６ｕ／ｍｌ。对菌株Ｂ—９５４５的产酶特性研究表明：该酶对高ｐＨ环境有较强的适应性,因此,可用于碱性洗涤用酶。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.