OPTIMIZATION OF THE ENUMERATION OF TOTAL AEROBIC BACTERIAL FLORA IN THE FLESH OF SEAFISH

Abstract The total aerobic flora of seafish flesh is weakly halophilic, and requires on average 1.38% NaCl according to statistical studies. Enumeration is optimal on tryptone soya agar or on NaCl supplemented plate count agar (-H₂S), incubated at 20 and 25C, respectively. Plate count agar (-H₂S) was selected because it can also be used for enumeration of hydrogen sulfide-producing bacteria by degradation of sulfur-containing proteins, which are abundant in fish The models employed are sigmoidal. The initial bioburden is too great for there to be a lag phase during storage in ice at 0C. The models show that when the total aerobic microflora count exceeds 100,000 cfu/g, whole or filleted fish stored on ice at 0C are unfit for consumption.     

乳酸杆菌体内外对阴道菌群及pH值生物学影响的研究

本研究利用从正常妇女阴道内分离出来的乳酸杆菌,从微生态学角度进行体内外生物拮抗试验,以探索其对阴道菌群及生境ｐＨ的生物学影响。结果表明：乳酸杆菌于体内外对阴道的葡萄球菌、肠杆菌、酵母菌有生物抑制作用;对生境的ｐＨ有生物学影响,主要表现为使生境ｐＨ下降。通过上述生物学影响调节生境菌群平衡及内环境的稳定。     

THE EFFECT OF FREEZING AND STORAGE ON THE BACTERIAL FLORA OF PASTEURIZED MILK

SUMMARY: Experiments were designed to determine the effect of freezing and storage on the bacterial population of pasteurized milk. Samples taken on separate occasions sometimes reacted differently to freezing treatment. Significant changes in bacterial numbers occurred after the frozen milk had been stored for 4–5 months at −12°, and this coincided with definite physical changes in the milk. Known numbers of coliaerogenes organisms were added to the pasteurized product in order to study their behaviour when frozen and stored at low temperatures. The counts were significantly lower after storage for 4 months.     

部分正常菌群分离菌株的超氧化歧化酶活性的测定

为了进一步研究部分正常菌群的生理和病理机制,我们测定了这些菌株的SOD活性和脂质过氧化物(LPO)变化,发现皮肤常住菌的分离株的混合培养时,SOD活性增加,说明它们具有协同作用。此外,对其它菌株SOD活性测定结果可知,类杆菌和双歧杆菌的SOD活性最低,其它菌株也有一定的SOD活性,如乳杆菌的SOD活性为47.47～98.98μg/ml,大肠杆菌的SOD活性为127.12μg/ml肠球菌的SOD活性为172.5μg/ml。     

淤积性皮炎与皮肤菌群关系的研究

目的：探讨淤积性皮炎与皮肤菌群之间的关系。方法：对21例小腿淤积性皮炎患者皮损，邻近皮肤及鼻孔的皮肤菌群进行检测。并将结果同20例正常人小腿皮肤及鼻孔菌群做了比较，其数据进行统计学处理。结果：金黄色葡萄球菌为淤积性皮炎患者皮肤的主要菌种，其分离率及密度均高于正常对照组。结论：金黄色葡萄球菌可能是淤积性皮炎发生的一个重要因素。     

广西植物区系的特有现象

本文统计了广西植物区系的特有属和特有种．分析了广西植物区系的特有现象．结果表明．广西植物区系有中国种子植物特有属６１个．占中国种子植物特有属总数的２３．７％;其中广西本地特有属１２个．特有程度仅次于有１５个省级特有属的云南。广西植物区系共有６９６个被子植物特有种．隶属于１０４科２８５属．它们有着强烈的热带亚热带性质．石灰岩山地的特有化发展则是广西植物区系的另一个显著特征,在６９６个特有种中．有２３０种为石灰岩山地专性特有种。这种石灰岩的特有化发展在苦苣苔科中表现得尤为显著。     

翘嘴鳜烂鳃病病原的研究

从患典型烂鳃病的翘嘴鳜（Siniperca chuatisi）鳃上分离到6个菌株,从中筛选出毒性最强的SC-3,SC-6两菌株。通过生物学特性观察、生理生化特性分析和致病力测试的结果,认为SC-3、SC-6为柱状屈挠杆菌（Flexibacter columnaris）,是翘嘴鳜烂鳃病的病原菌。药物敏感试验表明,SC-3对多种抗生素、呋喃类药物敏感而对磺胺噻唑不敏感。     

山西中条山植物区系的特征

中条山植物种类丰富,含种子植物１３２科,４７９属,９３５种。中条山植物区系起源古老。属的地理成分复杂,以温带成分占优势（２９５属,占６９．０９％）,反映出中条山植物区系的温带性质。中条山地处暖温带南部,成为热带、亚热带分布植物与温带分布植物的交汇地和由西南向东北分布的通道,说明中条山植物区系显著的过渡性。