共查询到20条相似文献,搜索用时 15 毫秒
1.
C.H. Spilman D.C. Beuving A.D. Forbes F.A. Kimball 《Prostaglandins & other lipid mediators》1977,14(3):477-488
The effects of prostaglandin (PG)F2α and PGF2α, 1–15 lactone were compared in luteal phase, non-pregnant and in early pregnant rhesus monkeys. Animals treated with either PG after pretreatment with human chorionic gonadotropin (hCG) had peripheral plasma progesterone concentrations that were not statistically different from those in animals treated with hCG and vehicle. However, menstrual cycle lengths in monkeys treated with PGF2α, 1–15 lactone were significantly (P <0.02) shorter than those in vehicle treated animals. In the absence of hCG pretreatment, plasma progesterone concentrations were significantly (P <0.008) lower by the second day after the initial treatment with either PGF2α or PGF2α, 1–15 lactone than in vehicle treated monkeys. Menstrual cycle lengths in monkeys treated with either PG were significantly (P <0.04) shorter than those in animals treated with vehicle. There were no changes in plasma progesterone concentrations in early pregnant monkeys treated with PGF2α, and pregnancy was not interrupted. In contrast, plasma progesterone declined and pregnancy was terminated in 5 of 6 early pregnant monkeys treated with PGF2α, 1–15 lactone. These data indicate that PGF2α, 1–15 lactone decreases menstrual cycle lengths in non-pregnant rhesus monkeys. More importantly, PGF2α, 1–15 lactone terminates early pregnancy in the monkey at a dose which is less than an ineffective dose of PGF2α. 相似文献
2.
J.C. Cornette K.T. Kirton W.P. Schneider F.F. Sun R.A. Johnson E.G. Nidy 《Prostaglandins & other lipid mediators》1975,9(2):323-338
Radioimmunoassay systems are described which have been developed to quantitate two principle urinary metabolites of PGF2α; 9α,11α-dihydroxy-15-oxo-2,3,4,5-tetranorprostanoic acid (I) and 9α-11α-dihydroxy-15-oxo-2,3,4,5-tetranorprosta-1,20-dioic acid (II). Preparation of the required metabolites was achieved by total synthesis (I) or by bioconversion (isolation from urine of animals treated with 15-keto-PGF2α*, II). These metabolites were used to prepare conjugates for immunization. Labeled metabolites, suitable as binding markers, were prepared by metabolism of 3H-PGF2α
(I) or
(II). Specificity of the resulting antibodies was compared to an antibody to PGF2α and to 13,14-dihydro-15-keto PGF2α. Antisera of II had little or no affinity for 20-carbon precursors (PGF2α or 13,14-dihydro-15-keto PGF2α), but had nearly equal affinity for metabolite I. Antisera of I, however, had little or no affinity for antigen of II. Therefore, analysis of samples by both assay systems enables quantitation of these excretion products of PGF2α. Other assay parameters (binding, affinity, recovery, precision and the repeatability of the assays) were similar to those previously described for other RIA systems, and were considered satisfactory for quanitation of compounds in biological fluids.Quantitation of 24 hour urinary excretion of di-acid metabolite in humans was in close agreement with previously published values determined by physical-chemical means. Greater quantity of di-acid metabolite was excreted by human males (42.0 μg/24 hr) than by females sampled either during the follicular (20.0) or luteal phase (21.2) of the menstrual cycle. The total quantity of C-16 metabolites (as approximated by system II) excreted/kg body weight by the rhesus monkey was similar to that excreted by the human. However, the ratio of di-acid to mono-acid was much nearer unity in the monkey than the human. 相似文献
3.
Shiro Ohki Yoshimasa Nishigaki Katsuhiro Imaki Masayasu Kurono Fumio Hirata Toshio Hanyu Nobuhiko Nakazawa 《Prostaglandins & other lipid mediators》1976,12(2):181-186
Radioimmunoassay technique for measuring 5α,7α-dihydroxy-11-keto-tetranorprosta-1,16-dioic acid, the main urinary metabolite of PGF1α and PGF2α (PGF2α-MUM), was further improved.It was postulated based on some experimental data that the PGF2α-MUM exists in the urine mostly as dioic acid form, not as δ-lactone formThe daily excretion of PGF2α-MUM in men ranged from 14.43 μg to 36.14 μg and in women from 5.21 μg to 14.25 μg. 相似文献
4.
Prostaglandin F2α (PGF2α) was measured by immunoassay in plasma and milk of four cows (six experiments). After 30 mg PGF2α im, plasma PGF2α peaked at 15 minutes (2.4 ± 0.7 ng/ml) and declined toward basal values by 3 hours; maximum milk PGF2α (0.91 ± 0.12 ng/ml) occurred at 1 hour. The average excretion rate in milk was 2.9 μg/day 0.9 μg (0.003%) of which was due to the 30 mg PGF2α injected. In six non-pregnant control cows, daily changes of milk PGF2α and progesterone were not consistently related. 相似文献
5.
John W. Wilks 《Prostaglandins & other lipid mediators》1977,13(1):161-170
The naturally-occurring metabolite of prostaglandin F2α, 15-keto prostaglandin F2α (15-keto PGF2α), elicited rapid and sustained declines in serum progesterone concentrations when administered to rhesus monkeys beginning on day 22 of normal menstrual cycles. Evidence for luteolysis of a more convincing nature was obtained in studies where a single dose of 15-keto PGF2α was given on day 20 of ovulatory menstrual cycles in which intramuscular injections of hCG were also given on days 18–20; serum progesterone concentrations fell precipitously in monkeys within 24 hours following intramuscular administration of 15-keto PGF2α. However, corpus luteum function was impaired in only 4 of 11 early pregnant monkeys when 15-keto PGF2α was administered on days 30 and 31 from the last menses, a time when the ovary is essential for the maintenance of pregnancy. Gestation failed in 2 additional monkeys 32 and 60 days after treatment with 15-keto PGF2α, but progressed in an apparently normal manner in the remaining 5 animals. Two pregnant monkeys treated with 15-keto PGF2α on day 42 from the last menstrual period, a time when the ovary is no longer required for gestation, continued their pregnancies uneventfully. Corpus luteum function was not impaired in 9 control monkeys which received injections of vehicle or hCG at appropriate times during the menstrual cycle or pregnancy. 相似文献
6.
Five guinea-pigs actively immunised against a PGF2α-bovine serum albumin conjugate showed elongated cycles, whereas 5 control animals injected with a mixture of PGF2α and bovine serum albumin showed no change in cycle length. These results are compatible with the hypothesis that the uterine luteolytic hormone in the guinea-pig is PGF2α. 相似文献
7.
Shiro Ohki Katsuhiro Imaki Fumio Hirata Toshio Hanyu Nobuhiko Nakazawa 《Prostaglandins & other lipid mediators》1974,6(2)
Radioimmunoassays for measuring prostaglandin F2α (PGF2α) and 5α, 7α-dihydroxy-11-keto tetranorprosta-1,16-dioic acid, PGF2α-main urinary metabolite (PGF2α-MUM), with 125I-tyrosine methylester amide (TMA) of PGF2α and PGF2α-MUM were developed.Antibody to PGF2α was produced in rabbits immunized with conjugates of PGF2α coupled to bovine serum albumine. Antibody to PGF2α-MUM was also produced in rabbits immunized with conjugates of PGF2α-MUM coupled to bovine serum albumin.PGF2α-125I-TMA had an affinity to antiserum to PGF2α. PGF2α-MUM-125I-TMA also responded to antiserum to PGF2α-MUM. 相似文献
8.
Martin A. Wasserman 《Prostaglandins & other lipid mediators》1975,9(6):959-973
The airway and lung dynamics of prostaglandin F2α (PGF2α) and three of its metabolites were examined in the spontaneously-ventilated, pentobarbital anesthetized dog. Changes in expiratory flow rate, tidal volume, respiration rate, lung resistance and dynamic lung compliance were evaluated and compared quantitatively. In a dose range of 0.3–3.0 μg/kg i.v., PGF2α and its 13,14-dihydro metabolite were found to be exceptionally potent agents. This metabolite was approximately twice as potent as PGF2α on most parameters studied. Two other metabolites, 15-keto-PGF2α and 15-keto-13,14-dihydro-PGF2α, were only slightly effective, even in a dose range of 1.0–30.0 μg/kg i.v. These latter two metabolites produced dose-response curves with significantly shallower slopes than PGF2α and were shown to be at least thirty-five times less potent than the parent compound. Therefore, oxidation of PGF2α at the carbon-15 position by 15-hydroxy prostaglandin dehydrogenase appears to produce compounds with minimal
bronchopulmonary activity. 相似文献
9.
3H5,6-Prostaglandin F1α is largely eliminated from the circulation during a single passage through the pulmonary vascular bed. Its elimination requires cellular uptake. The volume of distribution and the mean transit time of the radioactivity are greater than those of an intravascular marker, blue dextran. The lungs retain 25–30% of the radioactivity, most in the form of a metabolite less polar than PGF1α itself. The pulmonary venous effluent contains the same metabolite along with some unmetabolized PGF1α. The metabolite can be distinguished from prostaglandins of the A, B, D, E and F series. It is reduced on reaction with borohydride, but reduction does not yield PGF1α. Na0H has no effect on the metabolite, a finding that rules out the presence of a β-hydroxy-ketone configuration of the ring structure. The chromatographic behavior of the metabolite and its reaction with borohydride are those expected of 9,11-hydroxy-15-ketoprostanoic acid. 相似文献
10.
3H5,6-Prostaglandin F1α is largely eliminated from the circulation during a single passage through the pulmonary vascular bed. Its elimination requires cellular uptake. The volume of distribution and the mean transit time of the radioactivity are greater than those of an intravascular marker, blue dextran. The lungs retain 25–30% of the radioactivity, most in the form of a metabolite less polar than PGF1α itself. The pulmonary venous effluent contains the same metabolite along with some unmetabolized PGF1α. The metabolite can be distinguished from prostaglandins of the A, B, D, E and F series. It is reduced on reaction with borohydride, but reduction does not yield PGF1α. NaOH has no effect on the metabolite, a finding that rules out the presence of a β-hydroxy-ketone configuration of the ring structure. The chromatographic behavior of the metabolite and its reaction with borohydride are those expected of 9,11-hydroxy-15-ketoprostanoic acid. 相似文献
11.
The effect of prostaglandin PGF2α on the hCG stimulated and basal progesterone production by human corpora lutea was examined
. hCG (40 i.u./ml) stimulated progesterone formation in corpora lutea of early (days 16–19 of a normal 28 day cycle), mid (days 20–22) and late (days 23–27) luteal phases. This stimulation was inhibited by PGF2α (10 μg/ml) in corpora lutea of mid and late luteal phases. PGF2α alone did not show a consistent effect on basal progesterone production. The inhibition of hCG stimulated progesterone production by PGF2α at times corresponding to luteolysis indicates a role for that prostaglandin in the process of luteolysis in the human corpus luteum. 相似文献
12.
Jerome M. Feldman James W. Plonk James C. Cornette 《Prostaglandins & other lipid mediators》1974,7(6):501-506
Using specific radioimmunoassay procedures we measured prostaglandin F2α (PGF2α) and 13, 14-dihydro-15-keto prostaglandin F2α (PGF2α metabolite) in 12 patients with carcinoid tumors. Although PGF2α and PGF2α metabolite were each modestly elevated in 17% of the patients the magnitude of the elevation did not correlate with the symptoms of the carcinoid syndrome. The 24 hour urinary 5-hydroxyindoleacetic acid excretion showed a good correlation with carcinoid symptoms while the serum serotonin concentration showed a fair correlation with carcinoid symptoms. We conclude that serum elevation of PGF2α is not a frequent occurrence in patients with the carcinoid syndrome. 相似文献
13.
Prostaglandin F2α (PGF2α) at 14, 30 or 50 μg/hamster/day from Days 3–5 of pseudopregnancy (PSP) shortens PSP from a mean length of 9.1 to 5.6–7.9 days, depending on the dose of PGF2α administered. Bilateral intrauterine device (BIUD) bearing hamsters exhibit a mean length of PSP of 9.2 days, which is comparable to that in normal saline controls. Combination of PGF2α (14 μg/day on Days 3–5 of PSP) and BIUD also resulted in shortening of PSP although the mean length of PSP resulted from the combined treatment was not significantly different from those PSP animals treated with 14 μg/day of PGF2α alone. It is concluded that the antifertility effect of intrauterine device possibly is attributed to a small and continuous release of PGF which interferes with the normal implantation processes but does not curtail PSP. 相似文献
14.
Lars-Eric Edqvist Hans Kindahl George Stabenfeldt 《Prostaglandins & other lipid mediators》1978,16(1):111-119
Progesterone, estrone and 15-keto-13,14-dihydro-PGF2α levels were determined in the peripheral blood circulation during the peripartal period in 12 cows. Plasma concentrations of progesterone showed a gradual and continuous decrease during the last 60 days before parturition. This gradual decrease was followed by an abrupt decline in the progesterone concentration occurring 24–48 hours before delivery. The plasma levels of estrone started to increase about 30 days prior to parturition with high concentrations attained during the last days of pregnancy. After delivery the estrone content decreased to baseline levels. Increased levels of the PGF2α metabolite were recorded 24–48 hours before parturition. These increased PGF2α metabolite levels occurred before or in conjunction with prepartum luteolysis. Prostaglandin metabolite levels remained high during parturition and returned to baseline 10–20 days after delivery. 相似文献
15.
Shiro Ohki Katsuhiro Imaki Fumio Hirata Toshio Hanyu Nobuhiro Nakazawa 《Prostaglandins & other lipid mediators》1975,10(6):549-555
Radioimmunoassay of 5α,7α-dihydroxy-11-keto-tetranorprosta-1,16-dioic acid, main urinary metabolite of prostaglandin F2α (PGF2α), was performed using an antiserum produced in the rabbit.The antibody in 100 μ1 of 1,600-fold diluted antiserum binds with 60 picograms of metabolite.The main urinary metabolite level fell when flufenamic acid, a prostaglandin synthetase inhibitor, was given to rats. In contrast, it was significantly elevated when PGF2α was administered. 相似文献
16.
Burkhard Scherer Sven Fischer Wolfgang Siess Peter C. Weber 《Prostaglandins & other lipid mediators》1982,23(1):41-52
A radioimmunoassay (RIA) for the estimation of 6-keto-PGF1α in human urine is described in detail. The RIA method was validated by direct comparison to gas chromatography-mass spectrometry. In adults and in one year old children basal excretion of 6-keto-PGF1α was found to be lower than that reported for PGE2 or PGF2α. However, during the first week of life, significantly more 6-keto-PGF1α was excreted. The very high levels of 6-keto-PGF1α in urine seen on the third day of life seemed already to decrease during the first week of life. It is concluded that prostacyclin may have a major role for kidney function in the newborn, possibly by protecting the immature kidney from high levels of angiotensin II. 相似文献
17.
In humans eicosapentaenoic acid can be converted to 3-series prostaglandins (PGF3α, PGI3, and PGE3). Whether 3-series prostaglandins can protect the gastric mucosa from injury as effectively as their 2-series analogs is unknown. Therefore, we compared the protective effects of PGF3α and PGF2α against gross and microscopic gastric mucosal injury in rats. Animals received a subcutaneous injection of either PGF3α or PGF2α in doses raning from 0 (vehicle) to 16.8 μmol/kg and 30 min later they received intragastric administration of 1 ml of absolute ethanol. Whether mucosal injury was assessed 60 min or 5 min after ethanol, PGF3α was significantly less protective against ethanol-induced damage than PGF2α. These findings indicate that the presence of a third double bond in the prostaglandin F molecule between carbons 17 and 18 markedly reduces the protective effects of this prostaglandin on the gastric mucosa. 相似文献
18.
Francisco Gonzlez-Menci Bruce D. Murphy Jack Manns 《Prostaglandins & other lipid mediators》1977,14(3):535-542
Henderson and McNatty (Prostaglandins 9:779, 1975) proposed that LH from the preovulatory LH surge attached to receptors on luteal cells and that this attachment might protect the early corpus luteum from PGF2α induced luteolysis. To test this hypothesis, experiments were performed on heifers at day 10–12 of the cycle. Both jugular veins were catheterized and infusions of either saline (0.64 ml/min) or LH-NIH-B9 (10 μg/min; 0.64 ml/min) were given. Saline infusions were from 0–12 h; LH infusions were for 10 h and were preceded by a 2 h saline infusion. All animals were given 25 mg PGF2α im at 6 h (6 h into the saline infusion and 4 h into the LH infusion). Blood samples were taken at 0.5 h, 1 h and 4 h intervals from 0–12, 13–18 h and 22–24 h respectively. Serum was assayed for LH and progesterone by radioimmunoassay methods. Two animals received saline and two received LH in each experiment. Eact treatment was replicated 6 times. LH infusion resulted in a mean serum LH of 57 ng/ml compared to 0.90 ng/ml in saline infused animals. This elevation of LH did not alter PGF2α induced luteolysis as indicated by decline in serum progesterone. This experiment does not support the hypothesis that the newly formed corpus luteum is resistant to PGF2α because of protection afforded by the protestrus LH surge. 相似文献
19.
H. D. Hafs T. M. Louis R. J. Waters J. N. Stellflug N. B. Haynes 《Prostaglandins & other lipid mediators》1974,8(5):417-422
Seven rabbits were ejaculated four times once weekly, and saline or 2.5 mg PGF2α tromethamine salt was injected sc at 4 and 2 hours or at 8 and 4 hours before ejaculation. First ejacula taken at 2 hours after the second injection of PGF2α contained 150% more (P.07) sperm than those after injections of saline. The comparable difference (60%) at 4 hours after PGF2α was not significant. PGF2α did not influence sperm output in second, third or fourth ejacula. After 28 daily sc injections of 5 mg PGF2α in another experiment, the fertility of four treated rabbits was as high as that for four controls. Without sexual preparation in seven bulls, im injections of 40 or 80 mg PGF2α 30 minutes before ejaculation resulted in 33% greater (P<.05) sperm output than that after injection of 0, 7 or 20 mg PGF2α, but the highest sperm output after PGF2α was 30% less (P<.05) than that after sexual preparation in the same bulls. We conclude that injections of PGF2α result in increased sperm output in ejacula taken without sexual preparation within 2 hours in rabbits and in bulls. 相似文献
20.
Ray V. Haning Jr. Leslie Choi Amber J. Kiggens Donna L. Kuzma John W. Summerville 《Prostaglandins & other lipid mediators》1982,23(1):29-40
Explants from term human placentas were maintained in culture with daily changes of medium. Daily output of PGF2α and PGFM1 decreased during the course of the incubation. Addition of 4 μg/ml DHEAS or 67 μg/ml LDL cholesterol had no effect on output of PGF2α or PGFM. Addition of 1.6, 3.2, or 6.4 μg/ml of LHRH to the culture plates had no effect on output of PGFM or PGF2α, but LHRH increased hCG output. Dibutyryl cAMP (1mM, 2mM, and 4mM) increased output of PGF2α, PGFM, and hCG. Aromatase inhibitor decreased hCG output, but it was without effect on output of PGF2α, or PGFM. Significant correlations were demonstrated between progesterone, PGFM, PGF2α, and hCG, suggesting that PGF2α originates in the syncytiotrophoblast cell. The ability of LHRH to stimulate output of hCG but not PGF2α while dbcAMP stimulated both suggests that either PGF2α and hCG arise in different cells or that LHRH does not act through cAMP. 相似文献