小麦品种冀麦24抗麦红吸浆虫QTL分析

麦红吸浆虫是影响小麦产量和品质的重要害虫,研究小麦对吸浆虫抗性的遗传及其连锁分子标记对于提高抗虫品种的选择效率具有重要意义。本研究以小麦感虫品系6218与抗虫品种冀麦24产生的重组近交系(RIL)群体为材料,利用SSR标记和人工虫圃对冀麦24的抗虫性遗传进行了研究。结果表明:6218与冀麦24的抗性差异显著,RIL群体在2年2点的鉴定中抗性稳定;所构建的遗传连锁图谱包含112个SSR位点,形成26个连锁群,图谱全长835.7 cM,标记间平均距离为7.5 cM。利用QTL IciMapping的完备区间作图法,在4A染色体上检测到1个加性效应位点(QSm.hbau-4A),该位点在2个鉴定年度的贡献率分别为9.67%、10.57%。该抗性QTL及其连锁SSR标记的发掘,将有助于提高小麦抗吸浆虫育种的选择效率。     

南瓜遗传连锁图谱的构建及粒宽性状的QTL定位

以印度南瓜纯系大粒材料‘0515-1’和小粒材料‘0460-1-1’为亲本,获得193个南瓜F2单株群体,应用AFLP和SSR分子标记技术进行多态性筛选,构建了含84个标记位点的遗传连锁图谱。结果表明,整个图谱包含12个连锁群,全长683.50cM,标记平均间距为8.13cM。采用复合区间定位分析,共检测到控制南瓜籽粒宽度的4个数量性状位点(QTL),分别位于3个连锁群上,各QTL的贡献率在2.87%～29.68%之间。     

巴西橡胶树SSR遗传图谱的构建

以热研88-13×IAN873的94个F1群体为试材, 利用简单序列重复(Simple sequence repeat, SSR)标记, 采用FsLinkageMAP 1.0软件, 构建了巴西橡胶树热研88-13×IAN873的遗传连锁图谱。从441对SSR引物中筛选出160对具有多态信息的引物, 在分离群体中共检测到206个多态性位点, 176个位点用于遗传图谱的构建; χ²检验结果显示, 有147个位点符合1:1分离比例, 有12个符合1:2:1分离比例, 有17个符合1:1:1:1的分离比例, 共有13个偏分离位点, 偏分离率低(7.38%); 91个SSR位点被分为18个连锁群, 覆盖橡胶树基因组1 937.06 cM, 每个连锁群包含2～16个位点, 标记间的平均距离为21.29 cM。     

白桦RAPD遗传连锁图谱的构建

以80个来自欧洲白桦(Betula pendula Roth)×中国白桦(Betula platyphylla Suk)的F1个体为作图群体。利用2个亲本和10个F1个体对1,200个10 bp的随机寡核苷酸引物进行筛选, 确定了208个多态性引物。利用RAPD标记, 按照拟测交的作图策略, 分别构建了欧洲白桦和中国白桦的分子标记连锁图谱。对2个亲本和80个F1代作图群体进行随机扩增, 共获得了364个多态性位点。χ2检验结果表明有307个位点符合1∶1分离的拟测交分离, 26个位点符合3∶1分离, 31个位点属偏分离位点。拟测交位点中有145个位点来自欧洲白桦, 有162个位点来自中国白桦。利用2点连锁分析, 欧洲白桦中的145个连锁标记构成了14个不同的连锁群(4个以上标记), 6个三连体和6个连锁对, 37个为非连锁位点, 连锁标记覆盖的总图距为955.6 cM (centimorgan), 平均图距14.9 cM。而来自中国白桦的162个标记构成了15个连锁群(4个以上标记), 4个三连体和6个连锁对, 21个为非连锁位点, 连锁标记覆盖的总图距为1,545.8 cM (centimorgan), 平均图距15.2 cM。该图谱的建立为进一步将两个图谱整合为一个高密度图谱及重要基因的定位奠定了基础。     

梨分子遗传图谱构建及生长性状的QTL分析

利用鸭梨和京白梨杂交得到的F1(145株)实生苗为作图群体,通过对AFLP和SSR两种分子标记的遗传连锁分析,应用Joinmap 3.0作图软件,368个AFLP标记、34个SSR标记构建了分属18个连锁群的梨分子遗传连锁图谱,各连锁群的LOD值在4.0～7.0范围之间,图谱总长度覆盖梨基因组1395.9cM,平均图距为3.8cM.采用区间作图法,对该群体与生长性状相关的调查数据进行QTL分析,检测到与新梢生长量、新梢茎粗、节间长度、节间数量、树干径、树高及皮孔密度7个农艺性状连锁的QTL位点35个,其中主效QTL位点11个(LOD≥3.5).与生长性状相关的农艺性状QTL位点多集中在LG16连锁群上.     

小麦农家品种赤壳中一个主效抗条锈病基因的微卫星标记和定位

小麦农家品种赤壳(苏1900)对当前我国小麦条锈菌(Puccinia striiformis Westend.f.sp.tritici)多个流行小种均有较好抗性。遗传分析表明,该品种对条中32号小种的抗性是由一对显性基因控制。本文采用分离群体分析法(bulked segregant analysis,BSA)和微卫星多态性分析方法,对该基因进行了分子标记和定位研究。用Taichung29×赤壳的F2代分离群体建立抗、感DNA池,共筛选了400多对SSR引物,发现5个标记Xwmc44、Xgwm259、Xwmc367、Xcfa2292、Xbarc80在抗、感DNA池间与在抗、感亲本间同样具有多态性,它们均位于1BL染色体臂上。经用具有140株抗病株、60株感病株共200株植株的F2代分离群体进行的遗传连锁性检测,上述5个标记均与目的基因相连锁,遗传距离分别为8.3cM、9.1cM、17.2cM、20.6cM和31.6cM。用全套21个中国春缺-四体材料进行的检测进一步证实了这5个SSR标记均位于小麦1B染色体上。综合上述结果,将赤壳中的主效抗条锈病基因YrChk定位在1BL染色体臂上。与以前已定位于1B染色体上的抗条锈病基因的比较研究表明,YrChk基因可能是一个新的抗条锈病基因。小麦农家品种中抗病基因资源的发掘和利用将有助于提高我国小麦生产品种中的抗病基因丰富度,有助于改善长期以来小麦生产品种中抗病基因单一化的局面。     

拔节期与抽穗期玉米抗纹枯病相关QTL的初步定位

以玉米自交系R15(抗)×478(感)的F_2分离群体为作图群体,构建了包含146个SSR标记位点的遗传连锁图谱,覆盖玉米基因组1666 cM,平均图距11．4 cM。通过麦粒嵌入法对229个F_(2：4)家系进行人工接种纹枯病菌,于玉米拔节期和抽穗期进行纹枯病的抗性鉴定。应用复合区间作图法分析两个时期的抗病QTL及遗传效应。结果共检测到17个抗性QTL,其中以拔节期病情指数为指标共检测到9个QTL,分别位于第1、2、3、4、5、6、和10染色体上,可解释的表型变异为3．72%-9．26%;以抽穗期的病情指数为指标共在7条染色体上检测到10个抗玉米纹枯病的QTL,分布于第2、3、4、5、6、8和9染色体上。单个QTL可解释的表型变异为4．27%-9．27%。两个时期共检测出2个共同QTL,它们分别位于第2染色体的bnlgl662-bnlg1940区间和第6染色体的umc1006-umc1723区间。定位结果表明两个时期检测出的抗性QTL的差异表达与玉米不同发育时期基因的时空表达有密切关系,从而反映在纹枯病的抗性位点差异性上．这为玉米抗病选育提供新的信息。     

黄瓜SRAP遗传连锁图的构建及侧枝基因定位

在由黄瓜的两个自交系S06与S52杂交产生的F₂群体中, 应用SRAP(sequence-related amplified polymorphism)标记构建遗传连锁图谱, 检测控制黄瓜侧枝数(lbn)和侧枝平均长度(lbl)的数量性状座位(QTL). 使用筛选出的64个多态性引物组合对F2群体进行分析, 得到108个多态性位点. 经MAPMAKER/EXP3.0分析(LOD≥3.0), 获得由92个标记座位组成、覆盖7个连锁群的遗传图谱, 总长1164.2 cM, 标记平均间距12.6 cM. 应用QTLMapper1.6, 各检测到4个控制lbn和lbl的QTL, 其中, 对lbn贡献率最大的QTL位于第二连锁群的ME11SA4B-ME5EM5区间, 其S06基因型具有增效作用; 对lbl贡献率最大的QTL位于第二连锁群的DC1OD3-DC1EM14区间, 其S06基因型具有增效作用.     

利用重组自交系群体检测水稻条纹叶枯病抗性基因及QTL分析

利用81个株系组成的Kinmaze(japonica)／DV85(indica)重组自交系(recombinant inbred lines,RIL)群体,采用苗期强迫饲毒的鉴定方法,以病情指数作为条纹叶枯病的表型值,鉴定亲本及81个RILs对水稻抗条纹叶枯病毒(rice stripe virus,RSV)的抗性。利用QTL Cartographer软件,对水稻条纹叶枯病抗性基因进行检测分析。检测到3个QTL位点：qStv1、qStv7、qStv11分别位于第1、7、11染色体上,各QTL的LOD值为2．44～3．83,贡献率为19．8％～30．9％。根据抗性基因加性效应的方向,在qStv7、qStv11位点上,亲本DV85存在抗条纹叶枯病增效基因,Kinmaze具有抗条纹叶枯病减效基因,而qStv1位点抗性基因效应来源正好相反。     

白桦AFLP遗传连锁图谱的构建

以80个中国白桦(Betula platyphylla Suk)×欧洲白桦(Betula pendula Roth)的F1个体为作图群体, 利用扩增片段长度多态性(Amplified fragment length polymorphism, AFLP)标记, 按照拟测交作图策略, 分别构建了中国白桦和欧洲白桦的分子标记遗传连锁图谱。从64对AFLP引物组合中筛选出34对多态性丰富的引物组合, 这些入选的引物组合在分离群体中共检测到451个多态性位点。χ2检验结果表明, 有362个位点符合1∶1分离(拟测交分离位点), 41个位点符合3∶1分离, 20个位点符合1∶3分离, 28个位点属偏分离位点。在符合拟测交分离的位点中, 201个位点来自中国白桦, 161个位点来自欧洲白桦。利用2点连锁分析, 来自中国白桦的201个标记构成了14个连锁群(4个以上标记), 10个三连体和14个连锁对, 45个为非连锁位点, 连锁标记覆盖的总图距为1 296.1 cM, 平均图距15.5 cM。而来自欧洲白桦的161个标记构成了17个不同的连锁群(4个以上标记), 8个三连体和4个连锁对, 15个为非连锁位点, 连锁标记覆盖的总图距为1 035.8 cM, 平均图距12 cM。     

Comparative analysis of multiple disease resistance in ryegrass and cereal crops

Ryegrass (Lolium spp.) is among the most important forage crops in Europe and Australia and is also a popular turfgrass in North America. Previous genetic analysis based on a three-generation interspecific (L. perenne x L. multiflorum) ryegrass population identified four quantitative trait loci (QTLs) for resistance to gray leaf spot (Magneporthe grisea) and four QTLs for resistance to crown rust (Puccinia coronata). The current analysis based on the same mapping population detected seven QTLs for resistance to leaf spot (Bipolaris sorokiniana) and one QTL for resistance to stem rust (Puccinia graminis) in ryegrass for the first time. Three QTLs for leaf spot resistance on linkage groups (LGs) 2 and 4 were in regions of conserved synteny to the positions of resistance to net blotch (Drechslera teres) in barley (Hordeum vulgare). One ryegrass genomic region spanning 19 cM on LG 4, which contained three QTLs for resistance to leaf spot, gray leaf spot, and stem rust, had a syntenic relationship with a segment of rice chromosome 3, which contained QTLs for resistance to multiple diseases. However, at the genome-wide comparison based on 72 common RFLP markers between ryegrass and cereals, coincidence of QTLs for disease resistance to similar fungal pathogens was not statistically significant.     

SSR-based genetic linkage map construction in pistachio using an interspecific F<Subscript>1</Subscript> population and QTL analysis for leaf and shoot traits

Pistachio is one of the most commercially important nut trees in the world. To characterize the genetic controls of horticultural traits and facilitate marker-assisted breeding in pistachio, we constructed an SSR-based linkage map using an interspecific F₁ population derived from a cross between the cultivar “Siirt” (Pistacia vera L.) and the monoecious Pa-18 genotype of Pistacia atlantica Desf. This population was also used for the first QTL analysis in pistachio on leaf and shoot characters. In total, 1312 SSR primers were screened, and 388 loci were successfully integrated into parental linkage maps. The Siirt maternal map contained 306 markers, while the “Pa-18” paternal map included 285 markers along the 15 linkage groups. The Siirt map spanned 1410.4 cM, with an average marker distance of 4.6 cM; the Pa-18 map covered 1362.5 cM with an average marker distance of 4.8 cM. Phenotypic data were collected during the growing seasons of 2015 and 2016 for four traits: leaf length (LL), leaf width (LW), leaf length/leaf width ratio (LWR), number of leaflet pairs (NLL), and young shoot color (YSC). A total of 17 QTLs were identified in the parental maps. Four QTLs for LL and LW were located on LG2 and LG4, while four QTLs for LWR ratio on LG13 and LG14, two QTLs for NLL and two QTLs for YSC were on LG7 and LG9, respectively, with similar positions in both parental maps. The SSR markers, linkage maps, and QTLs reported here will provide a valuable resource for future molecular and genetic studies in pistachio.     

Quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice (Oryza sativa).

Blast is an economically important disease of rice. To map genes controlling blast resistance, recombinant inbred lines (RIL) were developed from Khao Dawk Mali 105, an aromatic, blast-susceptible cultivar and the blast resistance donor, CT 9993-5-10-M (CT). A linkage map encompassing 2112 cM was constructed from 141 RILs using 90 restriction fragment length polymorphisms (RFLPs) and 31 simple sequence repeats (SSR). Virulent isolates of blast fungus were identified by screening differential host sets against 87 single-spore isolates collected from the north and northeast of Thailand. Fifteen virulent blast isolates were selected for leaf blast screening. Neck blast was evaluated both under natural conditions and controlled inoculations. Quantitative trait loci (QTLs) for broad resistance spectrum (BRS) to leaf blast were located on chromosomes 7 and 9. In particular, the QTL(ch9) was mapped near the Pi5(t) locus. The QTL(ch7) was located close to a previously mapped partial resistance QTL. Both loci showed significant allelic interaction. Genotypes having CT alleles at both QTL(ch7) and QTL(ch9) were the most resistant. Two neck-blast QTLs were mapped on chromosomes 5 and 6. The inconsistent map locations between the leaf and neck blast QTLs indicate the complexity of fixing both leaf and neck blast resistance. The coincidence of BRS and field resistance QTLs on chromosome 7 supports the idea that BRS may reflect the broad resistance spectrum to leaf blast in rice. These findings laid the foundation for the development of a marker-assisted scheme for improving Khoa Dawk Mali 105 and the majority of aromatic Thai rice varieties that are susceptible to blast.     

An integrative genetic linkage map of winter wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.)

We constructed a genetic linkage map based on a cross between two Swiss winter wheat (Triticum aestivum L.) varieties, Arina and Forno. Two-hundred and forty F₅ single-seed descent (SSD)-derived lines were analysed with 112 restriction fragment length polymorphism (RFLP) anonymous probes, 18 wheat cDNA clones coding for putative stress or defence-related proteins and 179 simple-sequence repeat (SSR) primer-pairs. The 309 markers revealed 396 segregating loci. Linkage analysis defined 27 linkage groups that could all be assigned to chromosomes or chromosome arms. The resulting genetic map comprises 380 loci and spans 3,086 cM with 1,131 cM for the A genome, 920 cM for the B genome and 1,036 cM for the D genome. Seventeen percent of the loci showed a significant (P < 0.05) deviation from a 1:1 ratio, most of them in favour of the Arina alleles. This map enabled the mapping of QTLs for resistance against several fungal diseases such as Stagonospora glume blotch, leaf rust and Fusarium head blight. It will also be very useful for wheat genetic mapping, as it combines RFLP and SSR markers that were previously located on separate maps. S. Paillard and T. Schnurbusch contributed equally to the work     

Microsatellite mapping of adult-plant leaf rust resistance gene <Emphasis Type="Italic">Lr22a</Emphasis> in wheat

This study was conducted to identify microsatellite markers (SSR) linked to the adult-plant leaf rust resistance gene Lr22a and examine their cross-applicability for marker-assisted selection in different genetic backgrounds. Lr22a was previously introgressed from Aegilops tauschii Coss. to wheat (Triticum aestivum L.) and located to chromosome 2DS. Comparing SSR alleles from the donor of Lr22a to two backcross lines and their recurrent parents showed that between two and five SSR markers were co-introgressed with Lr22a and the size range of the Ae. tauschii introgression was 9-20 cM. An F(2) population from the cross of 98B34-T4B x 98B26-N1C01 confirmed linkage between the introgressed markers and Lr22a on chromosome 2DS. The closest marker, GWM296, was 2.9 cM from Lr22a. One hundred and eighteen cultivars and breeding lines of different geographical origins were tested with GWM296. In total 14 alleles were amplified, however, only those lines predicted or known to carry Lr22a had the unique Ae. tauschii allele at GWM296 with fragments of 121 and 131 bp. Thus, GWM296 is useful for selecting Lr22a in diverse genetic backgrounds. Genotypes carrying Lr22a showed strong resistance to leaf rust in the field from 2002 to 2006. Lr22a is an ideal candidate to be included in a stack of leaf rust resistance genes because of its strong adult-plant resistance, low frequency of commercial deployment, and the availability of a unique marker.     

玉米SSR连锁图谱构建及叶面积的QTL定位

叶片是玉米进行光合作用的主要器官,叶面积的大小(尤其是穗三叶面积)对于玉米干物质的积累及产量形成起着至关重要的作用。研究玉米叶面积的遗传基础对于指导玉米高产育种具有理论意义。文章以两个叶面积差异显著的亲本478×武312为基础材料所构建的218个F8代的重组自交系为作图群体,构建了一张包含184个SSR标记的遗传连锁图谱,图谱总长度为2084.1cM,平均图距为11.3cM。通过两年的田间试验对玉米叶面积(穗三叶)进行了QTL定位分析。两年共定位到7个和叶面积相关的QTL位点,2006年定位到4个QTL位点;2007年定位到3个QTL位点。在第2染色体umc1542-umc1518标记区间发现一个主效QTL位点,该位点可以在两年同时检测到,两年分别解释12.5%和17.3%的表型变异。该位点能稳定地检测到且具有较大的贡献率,可能会在玉米叶面积分子标记辅助选择上有所应用。     

QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice

Field resistance is defined as the resistance that allows effective control of a parasite under natural field conditions and is durable when exposed to new races of that parasite. To identify the genes for field resistance to rice blast, quantitative trait loci (QTLs) conferring field resistance to rice blast in Japanese upland rice were detected and mapped using RFLP and SSR markers. QTL analysis was carried out in F₄ progeny lines from the cross between Nipponbare (moderately susceptible, lowland) and Owarihatamochi (resistant, upland). Two QTLs were detected on chromosome 4 and one QTL was detected on each of chromosomes 9 and 12. The phenotypic variation explained by each QTL ranged from 7.9 to 45.7% and the four QTLs explained 66.3% of the total phenotypic variation. Backcrossed progeny lines were developed to transfer the QTL with largest effect using the susceptible cultivar Aichiasahi as a recurrent parent. Among 82 F₃ lines derived from the backcross, resistance segregated in the expected ratio of resistant 1 : heterozygous 2 : susceptible 1. The average score for blast resistance measured in the field was 4.2 ± 0.67, 7.5 ± 0.51and 8.2 ± 0.66, for resistant, heterozygous and susceptible groups, respectively. The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively. The relationship to previously reported major genes and QTLs conferring resistance to blasts, and the significance of marker-assisted selection to improve field resistance, are discussed. Received: 8 June 2000 / Accepted: 24 November 2000     

Identification and introgression of QTLs implicated in resistance to sorghum downy mildew (Peronosclerospora sorghi (Weston and Uppal) C. G. Shaw) in maize through marker-assisted selection

Sorghum downy mildew caused by Peronosclerospora sorghi is a major disease of maize and resistance is under the control of polygenes which necessitated identification of quantitative-trait loci (QTLs) for initiating marker-assisted introgression of resistant QTLs in elite susceptible inbred lines. In the present study, QTLs for sorghum downy mildew (SDM) resistance in maize were identified based on cosegregation with linked simple sequence repeats in 185 F₂ progeny from a cross between susceptible (CM500-19) and resistant (MAI105) parents. F₃ families were screened in the National Sorghum Downy Mildew Screening Nursery during 2010 and 2011. High heritability was observed for the disease reaction. The final map generated using 87 SSR markers had 10 linkage groups, spanning a length of 1210.3 cM. Although, we used only 87 SSR markers for mapping, the per cent of genome within 20 cM to the nearest marker was 88.5. Three putative QTLs for SDM resistance were located on chromosomes 3 (bin 3.01), 6 (bin 6.01) and 2 (bin 2.02) using composite interval mapping. The locus on chromosome 3 had a major effect and explained up to 12.6% of the phenotypic variation. The other two QTLs on chromosomes 6 and 2 had minor effects with phenotypic variation of 7.1 and 2%. The three QTLs appeared to have additive effects on resistance. The QTLs on chromosomes 3 and 6 were successfully used in the marker-assisted selection programme for introgression of resistance to SDM in eight susceptible maize lines.     

Selecting a set of wild barley introgression lines and verification of QTL effects for resistance to powdery mildew and leaf rust

A set of 59 spring barley introgression lines (ILs) was developed from the advanced backcross population S42. The ILs were generated by three rounds of backcrossing, two to four subsequent selfings, and, in parallel, marker-assisted selection. Each line includes a single marker-defined chromosomal segment of the wild barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum), whereas the remaining part of the genome is derived from the elite barley cultivar Scarlett (H. vulgare ssp. vulgare). Based on a map containing 98 SSR markers, the IL set covers so far 86.6% (1041.5 cM) of the donor genome. Each single line contains an average exotic introgression of 39.2 cM, representing 3.2% of the exotic genome. The utility of the developed IL set is illustrated by verification of QTLs controlling resistance to powdery mildew (Blumeria graminis f. sp. hordei L.) and leaf rust (Puccinia hordei L.) which were previously identified in the advanced backcross population S42. Altogether 57.1 and 75.0% of QTLs conferring resistance to powdery mildew and leaf rust, respectively, were verified by ILs. The strongest favorable effects were mapped to regions 1H, 0–85 cM and 4H, 125–170 cM, where susceptibility to powdery mildew and leaf rust was decreased by 66.1 and 34.7%, respectively, compared to the recurrent parent. In addition, three and one new QTLs were localized, respectively. A co-localization of two favorable QTLs was identified for line S42IL-138, which holds an introgressed segment in region 7H, 166–181. Here, a reduction effect was revealed for powdery mildew as well as for leaf rust severity. This line might be a valuable resource for transferring new resistance alleles into elite cultivars. In future, we aim to cover the complete exotic genome by selecting additional ILs. We intend to conduct further phenotype studies with the IL set in regard to the trait complexes agronomic performance, malting quality, biotic stress, and abiotic stress.     

小麦“BS20×Fu3”DH群体SSR遗传图谱的构建及不育基因的QTL定位

以小麦光温敏核雄性不育系BS20×Fu3双单倍体(DH)群体的289个系为材料,从1112对SSR和EST-SSR引物中筛选出多态性引物243对,利用其中128个SSR和6个EST-SSR标记构建遗传连锁图谱,该图谱覆盖长度为2749.2 c M,分布在小麦的19个连锁群(除4D、6A),不同连锁群标记数为2~15个,长度在15.3~244.4 c M之间,平均长度为144.7 c M,标记之间平均遗传距离为17.4 c M。同时构建3个DNA池(包括恢复池、北京不育池和阜阳不育池),用分离群体分组分析法(BSA)对育性进行分析,筛选出的多态性引物为Wmc264、Wmc73、Xgwm350,分布在3A、5B、2A/7D染色体上。同时用混合线性复合区间作图法(MCIM)对育性进行QTL分析,当F7.5时,检测到6个主效QTL,用复合区间作图法(CIM)对育性进行QTL分析,当LOD值2.5时,共检测到13个主效QTL,两种方法检测到一致的QTL有3个,分别为1BL的Wmc365-cfa2129、2BS的Wmc602-Xgwm148和3AL的Wmc264a-cfa2262区间的QTL。综合BSA和QTL的结果,位于1BL、2BS和3AL上的小麦光温敏不育基因是真实的。