共查询到10条相似文献,搜索用时 20 毫秒
1.
Kou HP Li Y Song XX Ou XF Xing SC Ma J Von Wettstein D Liu B 《Journal of plant physiology》2011,168(14):1685-1693
Cytosine methylation is responsive to various biotic- and abiotic-stresses, which may produce heritable epialleles. Nitrogen (N)-deficiency is an abiotic stress being repeatedly experienced by plants. To address possible epigenetic consequences of N-deficiency-stress, we investigated the stability of cytosine methylation in rice (Oryza sativa L.) subsequent to a chronic (a whole-generation) N-deficiency at two levels, moderate (20 mg/L) and severe (10 mg/L), under hydroponic culture. MSAP analysis revealed that locus-specific methylation alteration occurred in leaf-tissue of the stressed plants (S0) experiencing either level of N-deficiency, which was validated by gel-blotting. Analysis on three non-stressed self-fed progenies (S1, S2 and S3) by gel-blotting indicated that ca. 50% of the altered methylation patterns in somatic cells (leaf) of the stressed S0 plants were recaptured in S1, which were then stably inherited to S2 and S3. Bisulfite sequencing of two variant MSAP loci with homology to low-copy retrotransposons on one stressed plant (S0) and its non-stressed progenies (S1 and S2) showed that whereas one locus exhibited limited and non-heritable CHH methylation alteration, the other locus manifested dramatic heritable hypermethylation at nearly all cytosine sites within the assayed region. Intriguingly, when two groups of S2 plants descended from the same N-deficiency-stressed S0 plant were re-subjected to the stress, the group inheriting the modified methylation patterns showed enhanced tolerance to the N-deficiency-stress compared with the group bearing the original patterns. Our results thus demonstrate heritability of an acquired adaptive trait in rice, which was accompanied by epigenetic inheritance of modified cytosine methylation patterns, implicating an epigenetic basis underlying the inheritance of an acquired trait in plants. 相似文献
2.
Pavel Merkulov Sofya Gvaramiya Maxim Dudnikov Roman Komakhin Murad Omarov Alina Kocheshkova Zakhar Konstantinov Alexander Soloviev Gennady Karlov Mikhail Divashuk Ilya Kirov 《植物学报(英文版)》2023,65(10):2242-2261
Transposable element insertions (TEIs) are an important source of genomic innovation by contributing to plant adaptation, speciation, and the production of new varieties. The often large, complex plant genomes make identifying TEIs from short reads difficult and expensive. Moreover, rare somatic insertions that reflect mobilome dynamics are difficult to track using short reads. To address these challenges, we combined Cas9-targeted Nanopore sequencing (CANS) with the novel pipeline NanoCasTE to trace both genetically inherited and somatic TEIs in plants. We performed CANS of the EVADÉ (EVD) retrotransposon in wild-type Arabidopsis thaliana and rapidly obtained up to 40× sequence coverage. Analysis of hemizygous T-DNA insertion sites and genetically inherited insertions of the EVD transposon in the ddm1 (decrease in DNA methylation 1) genome uncovered the crucial role of DNA methylation in shaping EVD insertion preference. We also investigated somatic transposition events of the ONSEN transposon family, finding that genes that are downregulated during heat stress are preferentially targeted by ONSENs. Finally, we detected hypomethylation of novel somatic insertions for two ONSENs. CANS and NanoCasTE are effective tools for detecting TEIs and exploring mobilome organization in plants in response to stress and in different genetic backgrounds, as well as screening T-DNA insertion mutants and transgenic plants. 相似文献
3.
DNA methylation changes detected by methylation-sensitive amplified polymorphism in two contrasting rice genotypes under salt stress 总被引:1,自引:0,他引:1
DNA methylation,one of the most important epigenetic phenomena,plays a vital role in tuning gene expression during plant development as well as in response to environmental stimuli.In the present study,a rnethylation-sensitive amplified polymorphism (MSAP) analysis was performed to profile DNA methylation changes in two contrasting rice genotypes under salt stress.Consistent with visibly different phenotypes in response to salt stress,epigenetic markers classified as stable inter-cultivar DNA methylation differences were determined between salttolerant FL478 and salt-sensitive IR29.In addition,most tissue-specific DNA methylation loci were conserved,while many of the growth stage-dependent DNA methylation loci were dynamic between the two genotypes.Strikingly,salt stress induced a decrease in DNA methylation specifically in roots at the seedling stage that was more profound in IR29 than in the FL478.This result may indicate that demethylation of genes is an active epigenetic response to salt stress in roots at the seedling stage,and helps to further elucidate the implications of DNA methylation in crop growth and development. 相似文献
4.
In contrast to activated human T cells, activated mouse T cells fail to express MHC class II molecules (MHC-II) at their cell
surface. This is because mouse T cells hardly produce mRNA encoding the MHC-II molecules I-A and I-E, due to severely impaired
expression levels upon T-cell activation of the mhc2ta gene, encoding the class II transactivator (CIITA). In humans, activated T cells express exclusively the CIITA promoter III
(CIITA-PIII) isoform, which results in cell surface expression of all MHC-II isotypes (HLA-DR, -DP and -DQ). In this study,
we demonstrate that methylation of CIITA-PIII contributes to the failure of mouse T cells to transcribe the mhc2ta and the resulting I-A/E genes, explaining the lack of I-A/E molecule expression at the cell surface following activation. 相似文献
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SHEN Sile WANG Zhenwei SHAN Xiaohui WANG Hua LI Ling LIN Xuyun LONG Likun WENG Kenan LIU Bao ZOU Guangtian 《中国科学C辑(英文版)》2006,49(2):97-104
By using high-pressure treatment, two mutant lines were obtained from a genetically stable japonica rice cultivar Bijing38. Genomic DNA of the mutant lines, together with the original line (Bijing38), was either undigested
or digested by Hpa II/Msp I, and then subjected to molecular analysis using two markers, ISSR and RAPD. Results indicated that changes in the PCR amplification
profiles of both markers are apparent in the two mutant lines compared with the original rice cultivar, suggesting that there
had been both sequence changes and DNA methylation modifications in the mutant lines. Southern blot analysis using diverse
sequences, including two cellular genes (S2 and S3), a set of retrotransposons (Osr7, Osr36, Tos19 and more), and a MITE transposon family (mPing and Pong), confirmed the results, and indicated that changes in DNA methylation pattern, genomic structure, and possible activation
of some transposons indeed occurred in the mutant lines. Moreover, these changes are stably maintained through selfed generations
and in different organs. Thus, our results indicate that it is possible to obtain stable mutants in rice by high pressure
treatments, and the molecular basis of the mutants may include both genetic and epigenetic changes. Therefore, high hydrostatic
pressure seems a promising approach for plant mutagenesis. 相似文献
7.
Alterations in DNA methylation and genome structure in two rice mutant lines induced by high pressure 总被引:2,自引:0,他引:2
Sile Shen Zhenwei Wang Xiaohui Shan Hua Wang Ling Li Xuyun Lin Likun Long Kenan Weng Bao Liu Guangtian Zou 《中国科学:生命科学英文版》2006,49(2):97-104
Pressure is expected to be an important parameter to affect characteristics of matters and control rate and equilibrium of chemical reactions. As a fundamental thermodynamic variable, it also has effects on bio-macromolecules and a lot of physiological an… 相似文献
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9.
Lanjuan Hu Ning Li Zhibin Zhang Xinchao Meng Qianli Dong Chunming Xu Lei Gong Bao Liu 《The Plant journal : for cell and molecular biology》2020,101(1):188-203
CG methylation (mCG) is essential for preserving genome stability in mammals, but this link remains obscure in plants. OsMET1‐2, a major rice DNA methyltransferase, plays critical roles in maintaining mCG in rice. Null mutation of OsMET1‐2 causes massive CG hypomethylation, rendering the mutant suitable to address the role of mCG in maintaining genome integrity in plants. Here, we analyzed mCG dynamics and genome stability in tissue cultures of OsMET1‐2 homozygous (?/?) and heterozygous (+/?) mutants, and isogenic wild‐type (WT). We found mCG levels in cultures of ?/? were substantially lower than in those of WT and +/?, as expected. Unexpectedly, mCG levels in 1‐ and 3‐year cultures of ?/? were 77.6% and 48.7% higher, respectively, than in shoot, from which the cultures were initiated, suggesting substantial regain of mCG in ?/? cultures, which contrasts to the general trend of mCG loss in all WT plant tissue cultures hitherto studied. Transpositional burst of diverse transposable elements (TEs) occurred only in ?/? cultures, although no elevation of genome‐wide mutation rate in the form of single nucleotide polymorphisms was detected. Altogether, our results establish an essential role of mCG in retaining TE immobility and hence genome stability in rice and likely in plants in general. 相似文献
10.
We have reported recently that tissue culture induced a high level of genetic variation at the primary nucleotide sequence
in regenerants of medicinal plant Codonopsis lanceolata. It is not known, however, whether epigenetic variation in the form of alteration in DNA methylation also occurred in these
plants. Here, we investigated possible alterations in level and pattern of cytosine methylation at the CCGG sites in the same
set of regenerants relative to the donor plant, by the MSAP method employing a pair of isoschizomers, HpaII and MspI, which recognize the same restriction site but are differentially sensitive to cytosine methylation at the CCGG sites. A
total of 1,674 MSAP profiles were resolved using 39 primer combinations. Of these, 177 (10.5%) profiles were polymorphic among
the regenerants and/or between the regenerant(s) and the donor plant, in EcoRI + HpaII or EcoRI + MspI digest but not in both, indicating alteration in cytosine methylation patterns of specific loci, though their estimated
total level of methylation remained more or less the same as the donor plant. Gel blot analysis validated most of the variant
MSAP profiles as bona fide alteration in methylation patterns. Correlation analysis between the MSAP data and the previously
reported ISSR and RAPD data revealed significant correlations, suggesting their possible intrinsic interrelatedness. Thirty-seven
typical variant MSAP profiles were isolated and sequenced, of which 5 showed significant homology to known-function genes,
2 to chloroplast sequences, whilst the rest 30 did not find a match in the database.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
W. L. Guo and R. Wu contributed equally to this work. 相似文献