共查询到20条相似文献,搜索用时 15 毫秒
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Cho HJ Park J Lee HW Lee YS Kim JB 《Biochemical and biophysical research communications》2004,321(4):942-948
Here, we demonstrated that inhibition of mTOR with rapamycin has negative effects on adipocyte differentiation and insulin signaling. Rapamycin significantly reduced expression of most adipocyte marker genes including PPARgamma, adipsin, aP2, ADD1/SREBP1c, and FAS, and decreased intracellular lipid accumulation in 3T3-L1 and 3T3-F442A cells, suggesting that rapamycin would affect both lipogenesis and adipogenesis. Contrary to the previous report that suppressive effect of rapamycin on adipogenesis is limited to the clonal expansion, we revealed that its inhibitory effect persisted throughout the process of adipocyte differentiation. Thus, it is likely that constitutive activation of mTOR might be required for the execution of adipogenic programming. In differentiated 3T3-L1 adipocytes, chronic treatment of rapamycin blunted the phosphorylation of AKT and GSK, which is stimulated by insulin, and reduced insulin-dependent glucose uptake activity. Taken together, these results suggest that rapamycin not only prevents adipocyte differentiation by decrease of adipogenesis and lipogenesis but also downregulates insulin action in adipocytes, implying that mTOR would play important roles in adipogenesis and insulin action. 相似文献
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Li FQ Singh AM Mofunanya A Love D Terada N Moon RT Takemaru K 《Molecular and cellular biology》2007,27(12):4347-4354
The canonical Wnt/beta-catenin signaling pathway plays diverse roles in embryonic development and disease. Activation of this pathway, likely by Wnt-10b, has been shown to inhibit adipogenesis in cultured 3T3-L1 preadipocytes and in mice. Here, we report that the beta-catenin antagonist Chibby (Cby) is required for adipocyte differentiation. Cby is expressed in adipose tissue in mice, and Cby protein levels increase during adipogenic differentiation of 3T3-L1 cells. Ectopic expression of Cby induces spontaneous differentiation of these cells into mature adipocytes to an extent similar to that of dominant-negative Tcf-4. In contrast, depletion of Cby by RNA interference potently blocks adipogenesis of 3T3-L1 and mouse embryonic stem cells. In support of this, embryonic fibroblasts obtained from Cby-deficient embryos display attenuated differentiation to the adipogenic lineage. Mechanistically, Cby promotes adipocyte differentiation, in part by inhibiting beta-catenin, since gain or loss of function of Cby influences beta-catenin signaling in 3T3-L1 cells. Our results therefore establish Cby as a novel proadipogenic factor required for adipocyte differentiation. 相似文献
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Global mapping of cell type-specific open chromatin by FAIRE-seq reveals the regulatory role of the NFI family in adipocyte differentiation 总被引:1,自引:0,他引:1
Waki H Nakamura M Yamauchi T Wakabayashi K Yu J Hirose-Yotsuya L Take K Sun W Iwabu M Okada-Iwabu M Fujita T Aoyama T Tsutsumi S Ueki K Kodama T Sakai J Aburatani H Kadowaki T 《PLoS genetics》2011,7(10):e1002311
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Microarray analyses during adipogenesis: understanding the effects of Wnt signaling on adipogenesis and the roles of liver X receptor alpha in adipocyte metabolism 总被引:2,自引:0,他引:2
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Ross SE Erickson RL Gerin I DeRose PM Bajnok L Longo KA Misek DE Kuick R Hanash SM Atkins KB Andresen SM Nebb HI Madsen L Kristiansen K MacDougald OA 《Molecular and cellular biology》2002,22(16):5989-5999