共查询到20条相似文献,搜索用时 15 毫秒
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M. Garayoa A. C. Villaro U. Klein B. Zimmermann L. M. Montuenga P. Sesma 《Cell and tissue research》1995,282(2):343-350
The presence of a vacuolar-type ATPase in Malpighian tubules of the ant Formica polyctena was investigated immunocytochemically, using antibodies to vacuolar ATPases of Manduca sexta midgut and bovine kidney. Specific labelling was observed at the brush border of the epithelium extending along the entire length of the tubules. These findings agree with the current view that a vacuolar ATPase is situated at the apical membrane of Malpighian tubule cells and other insect epithelial cells, being the energizing element of an electrogenic potassium pump. When antibodies were tested on tubules in different secretion conditions prior to fixation, no differences were observed in the distribution of the vacuolar ATPase.This work was supported by grants from the European Community (SCI-CT90-0480), from the Ministerio de Educación y Ciencia DGICYT, Spain (CE 91-0002), and from the Deutsche Forschungsgemeinschaft (Wi 698-3). 相似文献
3.
Electrophoretic variation characterized by the presence (ES-5B+) or absence (ES-5B–) of esterase-5B in the plasma of the house mouse has been observed. It is suggested that the expression of esterase-5B is controlled by an autosomal locus, Esr, linked to Ldr-1 on chromosome 6, in addition to the presumptive structural locus Es-5, which is located on chromosome 8. A gene order of Lyt-3-Esr-Ldr-1 was determined by two crosses.Supported by the Deutsche Forschungsgemeinschaft (SFB 46).This is communication No. 33 of a research program devoted to the investigation of cellular distribution and genetics of nonspecific esterases. 相似文献
4.
The heredity and linkage of gene loci were established for two different enzymes with esterproteolytic activity from mouse submandibular gland: protease A and protease E. Based upon strain distribution and biochemical properties of the two esterproteases, the existence of two corresponding structural loci is proposed: Prt-4 (protease A) and Prt-5 (protease E). Prt-4 and Prt-5 proved to be different from Tam-1. From a four-point-cross, the gene order Gpi-1-(Tam-1, Prt-4, Prt-5)-c is suggested. Thus a gene cluster was shown to exist on chromosome 7 coding for esterproteases, all of which are controlled by testosterone.This work was supported by grants from the Deutsche Forschungsgemeinschaft, Bonn (SFB 46). 相似文献
5.
Scholten J Hartmann K Gerbaulet A Krieg T Müller W Testa G Roers A 《Transgenic research》2008,17(2):307-315
Mast cells are important effectors of type I allergy but also essential regulators of innate and adaptive immune responses.
The aim of this study was to develop a Cre recombinase-expressing mouse line that allows mast cell-specific inactivation of
genes in vivo. Following a BAC transgenic approach, Cre was expressed under the control of the mast cell protease (Mcpt) 5 promoter. Mcpt5-Cre transgenic mice were crossed to the ROSA26-EYFP Cre excision reporter strain. Efficient Cre-mediated recombination was observed
in mast cells from the peritoneal cavity and the skin while only minimal reporter gene expression was detected outside the
mast cell compartment. Our results show that the Mcpt5 promoter can drive Cre expression in a mast cell-specific fashion. We expect that our Mcpt5-Cre mice will be a useful tool for the investigation of mast cell biology.
Julia Scholten and Karin Hartmann contributed equally to this work.
Supported by grants from the German Research Counsil (Deutsche Forschungsgemeinschaft, RO 2133/2-2) to A.R. and K.H. and the
Koeln Fortune Program/Faculty of Medicine, University of Cologne, to A.R. and K.H.. The authors have no conflict of interest 相似文献
6.
Populations of the tunicate species Ciona intestinalis and Ascidiella aspersa were screened for electrophoretic polymorphism at various enzyme loci. Average heterozygosities are clearly related to population size as predicted by the neutral mutation hypothesis.This work was supported by the Deutsche Forschungsgemeinschaft. 相似文献
7.
The genetics of esterase-28, the major esterase of cauda epididymidis of the house mouse, has been studied after separation by polyacrylamide gel electrophoresis and isoelectric focusing. Four phenotypes are distinguished. Segregation ofEs-28 in two backcross series indicated linkage to Es-1, Es-9, and Es-22. The Es-28 locus was placed into esterase cluster 1 on chromosome 8.This work was supported by the Deutsche Forschungsgemeinschaft.This is communication No. 69 of a research program devoted to the cellular distribution, genetics, and regulation of nonspecific esterases. 相似文献
8.
Summary Partial hepatectomy was carried out on Xenopus laevis to investigate its influence on the endocrine system. In addition to other endocrine effects, a marked hypertrophy and stimulation of the interrenal gland was observed. Activated cells contain mitochondria with extended and irregularly coiled tubules embedded in a low electron dense matrix. Hepatectomy induces two phases of proliferation [3 and 35 days postoperative (p.o.)]. After 106 days p.o. giant mitochondria possessing narrow and closely packed, parallel tubules surrounded by an electron dense matrix indicate a phase of inactivation. The smooth endoplasmic reticulum and the Golgi apparatus proliferate after hepatectomy.During activation the high lipid content seen in controls is decreased significantly.This investigation was supported by the Deutsche Forschungsgemeinschaft 相似文献
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Genetic variation of a new codominantly inherited esterase, designated ES-17, has been discovered in the house mouse using
isoelectric focusing in polyacrylamide gels. The ES-17 A phenotype (three bands; isoelectric points, betweenpH 5.55 andpH 5.90) was found in C57 BL/10Sn. LP/J possessed the Es-17B phenotype (three bands; isoelectric points,pH 5.05–5.55). ES-17 was present in all tissues examined, except for hemolysate and serum, and was most clearly expressed in
the small intestine. Because of its reaction toward various substrates and inhibitors, ES-17 has tentatively been classified
as acetyl esterase (EC 3.1.1.6). ES-17 was shown to be controlled by the structural locusEs-17, located on chromosome 9. From test-cross data, a gene order ofEs-17-8.7±2.5 map units-Mpi-1-10.2±2.7 map units-Mod-1 was established.
This work was supported by the Deutsche Forschungsgemeinschaft (SFB 46). This is communication No. 35 of a research program
devoted to the cellular distribution and genetics of nonspecific esterases. 相似文献
11.
Muscular dysgenesis (mdg) in the mouse is a loss-of-function mutation of the skeletal muscle isoform of the voltage-sensor Ca2+ channel of skeletal muscle (DHP receptor alpha1 subunit, Cchl1a3, Chr1), which is essential for excitation-contraction coupling. Affected individuals (genotype mdg/mdg, phenotype MDG) are unable to breathe and die perinatally. We introduce here extraocular muscles in the study of MDG myopathy and show that, despite their developmental origin from head placodes, they are affected like trunk and limb muscles. MDG myotubes in situ are eliminated by necrosis, not apoptosis.The study was supported by the Deutsche Forschungsgemeinschaft, SFB 223 C03, E02 相似文献
12.
Summary The ontogeny of the chemoreceptive sensilla in the labial palp-pit organ was studied in Pieris rapae by examining twelve successive stages between pupation and emergence of the imago, which takes a period of 160 h under the experimental conditions. Mitoses occur until 20 h after pupation. They lead to anlagen of sensilla, 91% of which are comprised of three sensory cells. However, two sensory cells degenerate in each sensillum during a period of 28 h. The same process occurs in anlagen with four sensory cells resulting in bicellular sensilla. Axons grow out only after the number of sensory cells has been reduced. Further consecutive steps in sensory cell differentiation are: (a) outgrowth of dendritic outer segment and dendrite sheath; (b) outgrowth of trichogen process and change in structure of elongating dendrite sheath; (c) deposition of cuticle and pore tubules in the pegs; (d) retraction of trichogen process; (e) increase in diameter of dendritic outer segment accompanied by increase of microtubule number and appearance of regularly spaced electron-dense bodies at tubular doublets; (f) branching of dendritic outer segment; and (g) transformation of the dendritic branches into curled lamellae and partial destruction of the dendrite sheath. The unique process of sensory cell degeneration is interpreted as an event that revokes a step towards a possible functional improvement of the labial palp-pit organ during further evolutionSupported by the Deutsche Forschungsgemeinschaft (SFB 4/G1) 相似文献
13.
Summary (1) The basiconic sensilla on the antennae of Calliphora resemble other insect epidermal sensilla; one or several bipolar sense cells are surrounded by three non-neural cells. (2) The apical cell membrane of the tormogen cell(one of the three accessory cells) forms microvilli coated internally with particles. (3) In the (extracellular) outer receptor-lymph space hyaluronic acid can be demonstrated histochemically. (4) Demonstration of non-specific alkaline phosphatase, Mg2+-activated ATPase, and the presence of mitochondria in the apical part of the tormogen cell suggest active transport processes through these cells into the outer receptor-lymph space.Supported by the Deutsche Forschungsgemeinschaft 相似文献
14.
A further polymorphic rat esterase with broad tissue expression and restricted substrate specificity is described and tentatively called Es-6. Inbred rat strains have either fixed allele Es-6F or fixed allele Es-6S. Es-6 is not linked to the established esterase cluster consisting of the eight esterase loci Es-1, Es-2, Es-3M, Es-4M, Es-4W, Es-5 (=Es-3W), Es-7, and Es-8 in LG V of the rat or to RT1, Gc, c, a, and h. Esterases with apparently identical biochemical and genetical characteristics are Es-17 of the mouse and Es-A4 of humans.Supported by the Deutsche Forschungsgemeinschaft (Be 352/13 and Gu 105). 相似文献
15.
Growth phase and low pH affect the thermal regulation of the Yersinia enterocolitica inv gene 总被引:19,自引:3,他引:19
The inv gene encodes the protein invasin, which is the primary invasion factor for Yersinia enterocolitica in vitro and in vivo. Previous studies of Yersinia species have shown that inv expression and entry into mammalian cells are temperature regulated. Invasin production is reduced at the host temperature of 37°C as compared to production at ambient temperature; consequently, this study was initiated to determine whether other host environmental signals might induce inv expression at 37°C. An inv::phoA translational fusion was recombined on to the Y. enterocolitica chromosome by allelic exchange to monitor inv expression. Molecular characterization of expression of the wild-type inv gene and the inv phoA fusion showed that invasin is not produced until early stationary phase in bacteria grown at 23°C. Y. enterocolitica grown at 37°C and pH 5.5 showed levels of inv expression comparable to those observed in bacteria grown at 23 C. An increase in Na+ ions caused a slight increase in expression at 37 C. However, expression at 37°C was unaffected by anaerobiosis, growth’medium, calcium levels, or iron levels. Additionally, Y. enterocolitica expressed invasin in Peyer's patches two days after being introduced intragastrically into BALB/c mice. These results suggest that invasin expression in K enterocolitica may remain elevated eariy during interaction with the intestinal epithelium, a site at which invasin was shown to be necessary. 相似文献
16.
HSP68 — a DnaK-like heat-stress protein of plant mitochondria 总被引:1,自引:0,他引:1
D. Neumann M. Emmermann J.-M. Thierfelder U. zur Nieden M. Clericus H.-P. Braun L. Nover U. K. Schmitz 《Planta》1993,190(1):32-43
A 68-kDa heat-stress protein (HSP68) has been purified from cell-suspension cultures of tomato (Lycopersicon peruvianum L.). Antibodies raised against HSP68 cross-react with the Escherichia coli heat-stress protein DnaK. HSP68 was found to be a hydrophilic, ATP-binding protein. Immunological analysis of subcellular fractions and immunogold-labelling of ultrathin sections showed consistently that HSP68 is localized in the mitochondrial matrix. In-vitro translation experiments indicated that HSP68 is synthesized as a precursor protein. Immunoscreening of cDNA libraries from tomato and potato (Solanum tuberosum L.) led to the isolation of corresponding cDNA clones. The deduced amino-acid sequences show strong relationships to the DnaK-like proteins from bacteria and organelles of eukaryotic cells. The protein HSP68 is constitutively expressed, but its synthesis is increased during heat stress in all cells of higher plantes investigated so far.Abbreviations HSP
heat-stress protein
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
This work was supported by the Deutsche Forschungsgemeinschaft. 相似文献
17.
M. D. Sacristán F. Hoffmann 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1979,54(3):129-132
Summary A direct infection of cultured tissue with resting spores of the obligate endoparasitePlasmodiophora brassicae has been made possible by using stem embryo cultures of haploid rape. The fungus develops and completes its lifecycle in the cultured cells. The results are discussed in connection with the possibility of using this system to select for resistance to the pathogen.Supported by the Deutsche Forschungsgemeinschaft 相似文献
18.
In the population “Herrenmühle” ofChironomus plumosus 11% of the individuals contain one supernumerary chromosome. This B-chromosome is present both in germ-line and somatic cells.
— InChironomus melanotus 6% of the larvae of the population “Falkau” carry supernumerary chromosomes. These B-chromosomes cannot be found in all nuclei
of testis and soma, their number varies between cells within the individual. In both species the B-chromosomes represent centromeric
fragments of chromosome IV as can be shown by their structure and pairing behaviour. — The polytene B-chromosome ofCh. plumosus exhibits a banding pattern in the salivary gland nuclei. Furthermore it is able to form an additional nucleolus in the nuclei
of the malpighian tubules. InCh. melanotus band structures can be seen only in the B-chromosome of malpighian tubules. The larvae ofCh. melanotus, carrying B-chromosomes, show heterochromatic bodies in the salivary gland nuclei, varying in number and size in the nuclei
of the same gland. These bodies are interpreted to be polytenic B-chromosomes divided into subunits.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
19.
The major light-harvesting complexes from Mantoniella squamata (Prasinophyceae) and from Chlorella fusca (Chlorophyceae) were analyzed with respect to polypeptide composition and pigmentation. It was found that the polypeptides of Mantoniella are smaller than those of Chlorella and bind twice the amount of pigment. We assume that the amount of pigment per polypeptide is of ecological as well as of taxonomical importance.Abbreviations Chl
chlorophyll
- LHC
light-harvesting complex
- Xan
xanthophyll
We thank the support by the Deutsche Forschungsgemeinschaft. 相似文献
20.
B. Neumcke 《European biophysics journal : EBJ》1978,4(3):179-199
The present situation of 1/f noise in the passage of ions across membranes is examined. A survey of biological and synthetic membranes is given at which
a l/f frequency dependence has been observed in the spectrum of voltage or current fluctuations. Empirical relations and theories
of 1/f noise in membranes are critically discussed.
Supported by Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 38 “Membranforschung” 相似文献