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利用转基因植物生产口服疫苗的研究进展 总被引:2,自引:0,他引:2
人和动物的许多疾病是通过病原微生物与消化道、呼吸道和生殖道的表面粘膜接触而引起的〔1〕,如通过消化道传染的疾病有乙型肝炎、伤寒、霍乱、痢疾和胃肠炎等。在发展中国家 ,因为缺少资金购买或生产昂贵的疫苗和医疗设备 ,患病情况尤为严重。因此 ,急需生产价格低廉且不需要复杂医疗设备的口服疫苗。目前 ,利用转基因植物生产口服疫苗越来越受到重视〔1~ 12〕。这种技术已较成熟 ,主要涉及到口服疫苗抗原的选择、目标植物的选择、表达载体的构建、转化和检测及动物和人体试验。1 口服疫苗抗原的选择利用转基因植物生产的口服疫苗抗原一… 相似文献
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利用转基因植物生产乙肝口服疫苗 总被引:11,自引:0,他引:11
乙型肝炎危害全球大部分地区,由于没有切实可行的治疗方法,因此,控制和预防乙直炎便极其重要。转植物可表达外源乙肝表面抗原蛋白并保持自然状态的免疫一,产生的疫苗不需纯化就可供口服使用,所以,本文提出,利用转基因的生产廉价疫苗系统有巨大的前景,可代替微生物疫苗生产系统。 相似文献
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利用转基因植物作为生物反应器表达重组蛋白,生产外源蛋白质作为动物疫苗是一个很有吸引力的廉价生产系统,它有可能代替生产成本较高的传统疫苗的发酵生产系统。通过口蹄疫病毒VPI结构蛋白基因在转基因植物中的表达,口蹄疫疫苗已在植物中产生。在植物中生产的抗原能够保持其自身的免疫原性。本文简要综述了近十年来用转基因植物作为生物反应器生产口蹄疫疫苗的研究进展、特点及其应用前景。 相似文献
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本文概述了利用转基因植物生产口服疫苗的研究现状。分别对转基因植物生产口服疫苗的优点、作用原理、研究方法、已研究的口服疫苗及问题和前景进行了介绍。 相似文献
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利用转基因植物生产口服疫苗的研究现状 总被引:15,自引:0,他引:15
本文概述了利用转基因植物生产口服疫苗的研究现状,分别对转基因植物生产口服疫苗的优点、作用原理,研究方法,已研究的口服疫苗及问题和前景进行了介绍。 相似文献
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植物口服疫苗是通过转基因植物生产,通过口服的方式预防疾病的生物制品.作为一种新型疫苗,其研究开始于三十几年前.由于植物口服疫苗可以最大程度地降低传统疫苗的潜在风险,在疫苗生产中具有优势,因此拥有良好的商业生产前景.植物疫苗价格低廉,生产过程安全,可产生与注射疫苗相似效价效果,无论是在控制养殖业抗生素滥用的情况下作为替代... 相似文献
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转基因植物生产药用蛋白的研究进展 总被引:5,自引:0,他引:5
利用转基因植物作为生物反应器生产具有重要价值的多肽和蛋白质,包括抗体、疫苗、药用蛋白等较之其他生产系统具有很多优越性,已成为当前植物医药基因工程和药物生物技术领域中的研究热点,本文着重就这一领域近年采国内外的研究现状、发展趋势以及目前存在的问题及对策进行综述。 相似文献
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Plants have been identified as promising expression systems for commercial production of vaccine antigens. In phase I clinical trials several plant-derived vaccine antigens have been found to be safe and induce sufficiently high immune response. Thus, transgenic plants, including edible plant parts are suggested as excellent alternatives for the production of vaccines and economic scale-up through cultivation. Improved understanding of plant molecular biology and consequent refinement in the genetic engineering techniques have led to designing approaches for high level expression of vaccine antigens in plants. During the last decade, several efficient plant-based expression systems have been examined and more than 100 recombinant proteins including plant-derived vaccine antigens have been expressed in different plant tissues. Estimates suggest that it may become possible to obtain antigen sufficient for vaccinating millions of individuals from one acre crop by expressing the antigen in seeds of an edible legume, like peanut or soybean. In the near future, a plethora of protein products, developed through ‘naturalized bioreactors’ may reach market. Efforts for further improvements in these technologies need to be directed mainly towards validation and applicability of plant-based standardized mucosal and edible vaccines, regulatory pharmacology, formulations and the development of commercially viable GLP protocols. This article reviews the current status of developments in the area of use of plants for the development of vaccine antigens. 相似文献
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转基因植物作为生物反应器在疫苗生产中的应用 总被引:8,自引:0,他引:8
对转基因植物生产口服疫苗的发展、优点与作用机理、方法原理进行介绍,概述当前各种转基因植物疫苗的研究现状,指出了转基因植物疫苗研究面临的问题与前景 。 相似文献
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转基因植物疫苗研究策略 总被引:4,自引:0,他引:4
利用转基因植物作为生物反应器生产疫苗受到越来越多的关注。对转基因植物疫苗的研究、生产策略进行了介绍 ,重点对提高植物疫苗表达所采用的生物技术策略进行综述 ,探讨转基因植物疫苗的发展及面临的问题。 相似文献
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新一代转基因植物研究进展 总被引:12,自引:0,他引:12
转基因植物具有抗病、抗虫、抗逆、高产、营养成分改善等优良性状,但其安全性引起了人们的关注。新一代植物转基因技术,如叶绿体基因工程、基因约束、多基因共转、去除抗性标记基因、对外源基因进行实时监控、抗性管理策略、最小程度地改变基因等技术的发展,将使未来的转基因植物更好地适应人们的需求,更有利于消费者食用安全和生态环境的可持续发展 。 相似文献
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转基因植物表达植酸酶研究进展 总被引:6,自引:0,他引:6
植酸是植物体内磷的主要存在形式,其绝大部分不能被单胃动物消化吸收,而随粪便排出体外造成环境污染;同时,植酸又是一种抗营养因子,它通过络合植物体内的一些营养成分而降低植物的营养价值。通过植物转基因方法使植物自身表达足量的植酸酶,以减小植酸带来的不利影响,是提高植物性饲料营养价值和控制环境磷污染的一种经济有效的措施。就转基因植物植酸酶的优势、研究现状、存在的问题及其发展前景进行了综述。 相似文献
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Felipe Guadalupe Carrillo Landell Guillermina García Rivera Teresa de Jesús Olivera Flores Máximo B. Martínez Esther Orozco Orozco Miguel Angel Gómez Lim 《Plant Cell, Tissue and Organ Culture》2008,94(1):1-9
Several antigens from Entamoeba histolytica the agent causing human amoebiasis have been identified as potential vaccine candidates. One of them is the EhADH112 adhesin,
which plays an important role during trophozoite adherence to and phagocytosis of target cells. We report here the expression
of the EhADH112 carboxy-terminus of the gene (Adh240) in tomato plants. A 28 kDa band is recognized by specific antibodies in total proteins from transformed tomatoes. The plant-based
adhesin conserves the ability to inhibit trophozoite adherence and phagocytosis of target cells by an average of 32.8 and
44.75% respectively. 相似文献
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Expression of the rabies virus nucleoprotein in plants at high-levels and evaluation of immune responses in mice 总被引:1,自引:0,他引:1
Perea Arango I Loza Rubio E Rojas Anaya E Olivera Flores T Gonzalez de la Vara L Gómez Lim MA 《Plant cell reports》2008,27(4):677-685
Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins
including antibodies, antigens and hormones. Here, we report expression of a full-length nucleoprotein gene of rabies virus
in transgenic tomato plants. The nucleoprotein was also transiently expressed in Nicotiana benthamiana plants by agroinfiltration. In both cases, the nucleoprotein was expressed at high levels, 1–5% of total soluble protein
in tomato and 45% in N. benthamiana. Previously, only epitopes of the nucleoprotein had been expressed in plants. The presence and expression of the transgene
was verified by PCR, Southern, northern and western blots. Mice were immunized both intraperitoneally (i.p.) and orally with
tomato protein extracts containing the N protein induced the production of antibodies. The antibody titer of mice immunized
i.p., was at least four times higher than that of mice immunized orally. These results were reflected in the challenge experiments
where i.p.-immunized mice were partially protected against a peripheral virus challenge whereas orally immunized mice were
not. This protection was comparable to that obtained in previous experiments employing different expression systems. Work
is in progress to express both G and N proteins in transgenic plants and evaluate protection in mice. 相似文献