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1.
Haogang Zhang Ruichun Jia Chunjing Wang Tianming Hu Fujing Wang 《Biochemical and biophysical research communications》2014
Piceatannol, a naturally occurring analog of resveratrol, has been confirmed as an antitumor agent by inhibiting proliferation, migration, and metastasis in diverse cancer. However, the effect and mechanisms of piceatannol on colorectal cancer (CRC) have not been well understood. This study aimed to test whether piceatannol could inhibit growth of CRC cells and reveal its underlying molecular mechanism. 相似文献
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为探讨microRNA-152和microRNA-602检测在肝癌诊断及手术疗效评估中的应用价值。采用实时荧光定量PCR检测佳木斯市中心医院2012年3月~2015年3月的19例肝癌血清标本中microRNA-152和microRNA-602的表达水平,分析microRNA-152和microRNA-602在乙型肝炎病毒(HBV)阳性组、HBV阴性组和健康对照组血清样本中的表达差异。结果发现HBV阳性血清样本中microRNA-152的表达水平(0.65±0.29)明显低于健康组(1.21±0.32),microRNA-602在HBV阳性血清样本中的表达(0.63±0.31)明显高于健康组(0.44±0.15),且水平表达的差异具有统计学意义(p0.05)。可见血清样本中的microRNA-152和microRNA-602可以用于HBV阳性肝癌诊断的血清标记物,microRNA-152可以用于HBV阳性肝癌术后效果评价的血清标记物。 相似文献
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Background
Arachis hypogaea (peanut) is an important crop worldwide, being mostly used for edible oil production, direct consumption and animal feed. Cultivated peanut is an allotetraploid species with two different genome components, A and B. Genetic linkage maps can greatly assist molecular breeding and genomic studies. However, the development of linkage maps for A. hypogaea is difficult because it has very low levels of polymorphism. This can be overcome by the utilization of wild species of Arachis, which present the A- and B-genomes in the diploid state, and show high levels of genetic variability.Results
In this work, we constructed a B-genome linkage map, which will complement the previously published map for the A-genome of Arachis, and produced an entire framework for the tetraploid genome. This map is based on an F2 population of 93 individuals obtained from the cross between the diploid A. ipaënsis (K30076) and the closely related A. magna (K30097), the former species being the most probable B genome donor to cultivated peanut. In spite of being classified as different species, the parents showed high crossability and relatively low polymorphism (22.3%), compared to other interspecific crosses. The map has 10 linkage groups, with 149 loci spanning a total map distance of 1,294 cM. The microsatellite markers utilized, developed for other Arachis species, showed high transferability (81.7%). Segregation distortion was 21.5%. This B-genome map was compared to the A-genome map using 51 common markers, revealing a high degree of synteny between both genomes.Conclusion
The development of genetic maps for Arachis diploid wild species with A- and B-genomes effectively provides a genetic map for the tetraploid cultivated peanut in two separate diploid components and is a significant advance towards the construction of a transferable reference map for Arachis. Additionally, we were able to identify affinities of some Arachis linkage groups with Medicago truncatula, which will allow the transfer of information from the nearly-complete genome sequences of this model legume to the peanut crop. 相似文献5.
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Cong-Jun Wang Lin Tang Dong-Wei Shen Chao Wang Qiong-Ying Yuan Wei Gao Yong-Kun Wang Rong-Hua Xu Hui Zhang 《Molecular biology reports》2013,40(12):6525-6531
Hepatocellular carcinoma is a primary malignancy of hepatocytes which accounts for 80 % of all primary liver cancers. DFNA5 has been identified as a tumor suppressor gene with an important role in several frequent forms of cancers, while little is known about its role in hepatocellular carcinoma. Through comparison of the DFNA5 protein expression in hepatocellular carcinoma cells (HepG2) with human fetal lung fibroblast cells (MRC5), we found that the DFNA5 protein expression in hepatocellular carcinoma cells was significantly lower than that in normal cells. The transfection of DFNA5 gene into HepG2 cells could increase DFNA5 protein expression, which subsequently led to inhibition of cell proliferation. Underlying mechanism study revealed that decreased proliferation was due to increased apoptosis and cell cycle arrest. In view of the important role of DFNA5 gene in carcinogenesis, these findings are expected to provide new understanding on development and treatment of human hepatocellular carcinoma. 相似文献
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Cheng-Shyuan Rau Johnson Chia-Shen Yang Shao-Chun Wu Yi-Chun Chen Tsu-Hsiang Lu Ming-Wei Lin Yi-Chan Wu Siou-Ling Tzeng Chia-Jung Wu Ching-Hua Hsieh 《Journal of biomedical science》2013,20(1):64
Background
The lack of noninvasive biomarkers of rejection remains a challenge in the accurate monitoring of deeply buried nerve allografts and precludes optimization of therapeutic intervention. This study aimed to establish the expression profile of circulating microRNAs (miRNAs) during nerve allotransplantation with or without immunosuppression.Results
Balb/c mice were randomized into 3 experimental groups, that is, (1) untreated isograft (Balb/c → Balb/c), (2) untreated allograft (C57BL/6 → Balb/c), and (3) allograft (C57BL/6 → Balb/c) with FK506 immunosuppression. A 1-cm Balb/c or C57BL/6 donor sciatic nerve graft was transplanted into sciatic nerve gaps created in recipient mice. At 1, 3, 7, 10, and 14 d after nerve transplantation, nerve grafts, whole blood, and sera were obtained for miRNA expression analysis with an miRNA array and subsequent validation with quantitative real-time PCR (qRT-PCR). Three circulating miRNAs (miR-320, miR-762, and miR-423-5p) were identified in the whole blood and serum of the mice receiving an allograft with FK506 immunosuppression, within 2 weeks after nerve allotransplantation. However, these 3 circulating miRNAs were not expressed in the nerve grafts. The expression of all these 3 upregulated circulating miRNAs significantly decreased at 2, 4, and 6 d after discontinuation of FK506 immunosuppression. In the nerve graft, miR-125-3b and miR-672 were significantly upregulated in the mice that received an allograft with FK506 only at 7 d after nerve allotransplantation.Conclusions
We identified the circulating miR-320, miR-762, and miR-423-5p as potential biomarkers for monitoring the immunosuppression status of the nerve allograft. However, further research is required to investigate the mechanism behind the dysregulation of these markers and to evaluate their prognostic value in nerve allotransplantation. 相似文献8.
Yuwu Liu ;Chang Wu ;Ying Wang ;Sailan Wen ;Junpu Wang ;Zhihong Chen ;Qiongqiong He ;Deyun Feng 《Acta biochimica et biophysica Sinica》2014,(8):720-722
MicroRNAs (miRNAs) have been demonstrated to involve in multiple biological processes, including apoptosis, prolif- eration, differentiation, and metastasis [1-3]. In addition, several groups have reported that abnormalities of specific miRNAs are implicated in cancer pathogenesis [4,5]. The roles of miR-224 and miR-145 in tumorigenesis have attracted the attention of many researchers. The protein A disintegrin and metalloproteinase 17 (ADAM17) is over- expressed in many types of cancers, including hepatocellular carcinoma (HCC). ADAM17 expression is regulated by mul- tiple miRNAs, which have been identified in several types of cancers [6,7]. In silico analysis suggested that ADAM17 is a common target of both miR-224 and miR-145. In the present study, we investigated the expression and distribution ofmiR- 224, miR-145, and ADAM17 in HCC samples and analyzed the relationship between both miRNAs and ADAM17. 相似文献
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Iris Barshack Eti Meiri Shai Rosenwald Danit Lebanony Meital Bronfeld Sarit Aviel-Ronen Kinneret Rosenblatt Sylvie Polak-Charcon Ilit Leizerman Meital Ezagouri Merav Zepeniuk Norberto Shabes Lahav Cohen Sarit Tabak Dalia Cohen Zvi Bentwich Nitzan Rosenfeld 《The international journal of biochemistry & cell biology》2010,42(8):1355-1362
Distinguishing hepatocellular carcinoma from metastatic tumors in the liver is of great practical importance, with significant therapeutic and prognostic implications. This differential diagnosis can be difficult because metastatic cancers in the liver, especially adenocarcinomas, may mimic the morphology and immunoexpression of hepatocellular carcinoma. Biomarkers that are specifically expressed in either hepatocellular carcinoma or metastatic adenocarcinoma can therefore be useful diagnostic tools. To find such biomarkers, we studied microRNA expression in 144 tumor samples using custom microarrays. Hsa-miR-141 and hsa-miR-200c, microRNAs that promote epithelial phenotypes, had significantly higher levels in non-hepatic epithelial tumors. In contrast, endothelial-associated hsa-miR-126 showed higher expression levels in hepatocellular carcinomas. Combinations of these microRNAs accurately identified primary hepatocellular carcinoma from metastatic adenocarcinoma in the liver. These findings were validated using quantitative real-time PCR to measure microRNA expression in additional samples. Thus, the tissue-specific expression patterns of microRNAs make them useful biomarkers for the diagnosis of liver malignancies. 相似文献
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Ann K.Y. ToGeorge G. Chen Ursula P.F. ChanCaiguo Ye Jing P. YunRocky L.K. Ho Art TessierJuanita L. Merchant Paul B.S. Lai 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2011,1813(1):222-230
ZBP-89 can enhance tumor cells to death stimuli. However, the molecular mechanism leading to the inhibitory effect of ZBP-89 is unknown. In this study, 4 liver cell lines were used to screen for the target of ZBP-89 on cell death pathway. The identified Bak was further analyzed for its role in ZBP-89-mediated apoptosis. The result showed that ZBP-89 significantly and time-dependently induced apoptosis. It significantly upregulated the level of pro-apoptotic Bak. ZBP-89 targeted a region between -457 and -407 of human Bak promoter to stimulate Bak expression based on the findings of Bak promoter luciferase report gene assay and electrophoretic mobility shift assay. ZBP-89-induced Bak increase and ZBP-89-mediated apoptosis were markedly suppressed by Bak siRNA, confirming that Bak was specifically targeted by ZBP-89 to facilitate apoptosis. In conclusion, this study demonstrated that ZBP-89 significantly induced apoptosis of HCC cells via promoting Bak level. 相似文献
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Liu S Li Y Chen W Zheng P Liu T He W Zhang J Zeng X 《Biochemical and biophysical research communications》2012,419(4):656-661
Hepatocellular carcinoma (HCC) is one of the most common internal malignant tumors. Glypican-3 (GPC3) is involved in the biological and molecular events in the tumorigenesis of HCC. We used RNA interference to evaluate the molecular effects of GPC3 suppression at the translational level and demonstrated for the first time that GPC3 silencing results in a significant elevation of the Bax/Bcl-2 ratio, the release of cytochrome c from mitochondria and the activation of caspase-3. The results suggest that GPC3 regulates cell proliferation by enhancing the resistance to apoptosis through the dysfunction of the Bax/Bcl-2/cytochrome c/caspase-3 signaling pathway and therefore plays a critical role in the tumorigenesis of HCC. Thus, the knockdown of GPC3 should be further investigated as an attractive novel approach for the targeted gene therapy of HCC. 相似文献
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Background
MicroRNAs are non-coding small RNAs of ~22 nucleotides that regulate the gene expression by base-paring with target mRNAs, leading to mRNA cleavage or translational repression. It is currently estimated that microRNAs account for ~ 1% of predicted genes in higher eukaryotic genomes and that up to 30% of genes might be regulated by microRNAs. However, only very few microRNAs have been functionally characterized and the general functions of microRNAs are not globally studied. 相似文献14.
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Bihrer V Waidmann O Friedrich-Rust M Forestier N Susser S Haupenthal J Welker M Shi Y Peveling-Oberhag J Polta A von Wagner M Radeke HH Sarrazin C Trojan J Zeuzem S Kronenberger B Piiper A 《PloS one》2011,6(10):e26971
Background
MicroRNA-21 (miR-21) is up-regulated in tumor tissue of patients with malignant diseases, including hepatocellular carcinoma (HCC). Elevated concentrations of miR-21 have also been found in sera or plasma from patients with malignancies, rendering it an interesting candidate as serum/plasma marker for malignancies. Here we correlated serum miR-21 levels with clinical parameters in patients with different stages of chronic hepatitis C virus infection (CHC) and CHC-associated HCC.Methodology/Principal Findings
62 CHC patients, 29 patients with CHC and HCC and 19 healthy controls were prospectively enrolled. RNA was extracted from the sera and miR-21 as well as miR-16 levels were analyzed by quantitative real-time PCR; miR-21 levels (normalized by miR-16) were correlated with standard liver parameters, histological grading and staging of CHC. The data show that serum levels of miR-21 were elevated in patients with CHC compared to healthy controls (P<0.001); there was no difference between serum miR-21 in patients with CHC and CHC-associated HCC. Serum miR-21 levels correlated with histological activity index (HAI) in the liver (r = −0.494, P = 0.00002), alanine aminotransferase (ALT) (r = −0.309, P = 0.007), aspartate aminotransferase (r = −0.495, P = 0.000007), bilirubin (r = −0.362, P = 0.002), international normalized ratio (r = −0.338, P = 0.034) and γ-glutamyltransferase (r = −0.244, P = 0.034). Multivariate analysis revealed that ALT and miR-21 serum levels were independently associated with HAI. At a cut-off dCT of 1.96, miR-21 discriminated between minimal and mild-severe necroinflammation (AUC = 0.758) with a sensitivity of 53.3% and a specificity of 95.2%.Conclusions/Significance
The serum miR-21 level is a marker for necroinflammatory activity, but does not differ between patients with HCV and HCV-induced HCC. 相似文献17.
Pinger Wang Rui Dong Baoli Wang Zhaohuan Lou Jun Ying Chenjie Xia Songfeng Hu Weidong Wang Qi Sun Peng Zhang Qinwen Ge Luwei Xiao Di Chen Peijian Tong Ju Li Hongting Jin 《Journal of cellular physiology》2019,234(12):21877-21888
Emerging evidence suggests that microRNAs (miRNAs) may be pathologically involved in osteoarthritis (OA). Subchondral bone (SCB) sclerosis is accounted for the knee osteoarthritis (KOA) development and progression. In this study, we aimed to screen the miRNA biomarkers of KOA and investigated whether these miRNAs regulate the differentiation potential of mesenchymal stem cells (MSCs) and thus contributing to SCB. We identified 48 miRNAs in the blood samples in KOA patients (n = 5) through microarray expression profiling detection. After validation with larger sample number, we confirmed hsa-miR-582-5p and hsa-miR-424-5p were associated with the pathology of SCB sclerosis. Target genes prediction and pathway analysis were implemented with online databases, indicating these two candidate miRNAs were closely related to the pathways of pluripotency of stem cells and pathology of OA. Surprisingly, mmu-miR-582-5p (homology of hsa-miR-582-5p) was downregulated in osteogenic differentiation and upregulated in adipogenic differentiation of mesenchymal progenitor C3H10T1/2 cells, whereas mmu-mir-322-5p (homology of hsa-miR-424-5p) showed no change through the in vitro study. Supplementing mmu-miR-582-5p mimics blocked osteogenic and induced adipogenic differentiation of C3H10T1/2 cells, whereas silencing of the endogenous mmu-miR-582-5p enhanced osteogenic and repressed adipogenic differentiation. Further mechanism studies showed that mmu-miR-582-5p was directly targeted to Runx2. Mutation of putative mmu-miR-582-5p binding sites in Runx2 3′ untranslated region (3′UTR) could abolish the response of the 3′UTR-luciferase construct to mmu-miR-582-5p supplementation. Generally speaking, our data suggest that miR-582-5p is an important biomarker of KOA and is able to regulate osteogenic and adipogenic differentiation of MSCs via targeting Runx2. The study also suggests that miR-582-5p may play a crucial role in SCB sclerosis of human KOA. 相似文献
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El-Shal Amal S. Shalaby Sally M. Abouhashem Safwat E. Elbary Eman H. Abd Azazy Samir Rashad Nearmeen M. Sarhan Walaa 《Molecular biology reports》2021,48(5):4361-4371
Molecular Biology Reports - Because of low sensitivity and specificity of the currently available urine biomarkers of bladder cancer (BC) detection and painful cystoscopy procedure. Our study aimed... 相似文献
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