中国复殖吸虫凸腹科两新记录属并重新描述

在中国南海的矛尾翻车鲀（Masturus lanceolatus）中发现了两种复殖吸虫,Accacoelium contortum（Ruldolphi,1819）和Accacladocoelium petasiporum（Odhner,1928）,它们隶属于凸腹科（Accacoeliidae Odhner,1911）,这两种复殖吸虫的子宫走向和以前的描述不同,对它们的形态进行了重新描述。另外,研究中的A.contortum是在宿主肠道中发现,而不是如从前记录的发现在宿主的鳃上。这两种虫及其所在的属都是首次在中国发现和报道。     

The long-term survival of Escherichia coli in river water

Escherichia coli introduced into autoclaved filtered river water survived for up to 260 d at temperatures from 4° to 25°C with no loss of viability. Survival times were less in water which was only filtered through either a Whatman filter paper or a 0.45 μm Millipore filter or in untreated water, suggesting that competition with the natural microbial flora of the water was the primary factor in the disappearance of the introduced bacteria. Survival was also dependent upon temperature with survival at 4°C > 15°C > 25°C > 37°C for any water sample. Direct counts showed that bacterial cells did not disappear as the viable count decreased. The possession of the antibiotic resistance plasmids, R 1 drd -19 or R144-3, did not enhance survival nor cause a faster rate of decay, indicating that the metabolic burden imposed by a plasmid was not a factor in survival under starvation conditions. There was no evidence of transfer of either plasmid at 15°C or of loss of plasmid function during starvation.     

Effect of water temperature and size of females on the timing of spawning of perch Perca fluviatilis L. in Lake Geneva from 1984 to 2003

The effects of water temperature on the timing of spawning of perch Perca fluviatilis in Lake Geneva were assessed in a 20 year survey (1984–2003). Spawning started at the end of April or at the beginning of May, when the water temperature rose to >10° C, but most of the spawns were laid when water temperature exceeded 12° C. This led to a positive correlation between the date of the mid-spawning period and the date when the water temperature rose to >12° C. Large perch had a tendency to spawn later than the small ones, which led to a positive correlation between the date of mid-spawning period and the yearly mean width of perch egg-ribbons. The coefficient of multiple determination between the date of mid-spawning period and the mean width of the ribbon of spawn, combined with the date when the water temperature rose to >12° C was r ²= 0·77. The perch generally chose deeper spawning places (12 m) at the end of the spawning period than at the beginning (4 m), which was related to water temperature.     

Laboratory studies on the effects of thermal change on the behaviour and distribution of juvenile chum salmon in sea water

Schooling chum salmon Oncorhynchus keta were biased towards the water surface (median position <1 m) under isothermal conditions (10° C) in a water column simulator (WCS). Thermal stratification (24/10° C) inhibited upward movement with fish congregating at the thermocline and displaying a clear avoidance of potentially lethal surface waters. A tri-phase model based on piece-wise nonlinear regression was used to describe the distribution shifts of chum salmon during a change from isothermal to thermally stratified conditions. Fish distribution was consistent with thermoregulatory behaviour and exhibited 'attraction', 'preference' and 'avoidance' phases. The thermal preference of 50% of the fish lay between 12·2 and 20·2° C, however, >83·5% of the fish occupied a 'preferred' temperature range of 13·7–17·9° C. The mean temperature at which 50% of chum salmon avoided rising temperature by shifting deeper in the water column and using the cooler thermocline was 20·2° C, and 90% avoidance occurred at 22·9° C. Behavioural responses to thermal stratification were consistent amongst underyearling fish of differing size and age.     

The effect of temperature on the ability of Tilapia mossambica Peters to enter deep water

The ability of adult Tilapia mossambica Peters to enter deep water was determined at 15, 22 and 30°C. At 30°C adults compensate to about 20m depth but at 15°C to only 7 m. Compensation is more rapid at high than at low temperatures. T. mossambica haemoglobin has a marked Root effect which is the same at 22 and 30°C. The oxygen affinity of the haemoglobin is higher at 15°C than at 30°C. There was no measurable difference in the rate of passive oxygen diffusion across the swimbladder wall in the temperature range 15–30°C. It is concluded that the ability to enter deeper water at higher temperatures is related to decreased oxygen affinity of the haemoglobin and higher rates of oxygen secretion and blood circulation.     

Behavioural thermoregulation by subyearling fall (autumn) Chinook salmon Oncorhynchus tshawytscha in a reservoir

This study investigated behavioural thermoregulation by subyearling fall (autumn) Chinook salmon Oncorhynchus tshawytscha in a reservoir on the Snake River, Washington, U.S.A. During the summer, temperatures in the reservoir varied from 23° C on the surface to 11° C at 14 m depth. Subyearlings implanted with temperature-sensing radio transmitters were released at the surface at temperatures >20° C during three blocks of time in summer 2004. Vertical profiles were taken to measure temperature and depth use as the fish moved downstream over an average of 5·6–7·2 h and 6·0–13·8 km. The majority of the subyearlings maintained average body temperatures that differed from average vertical profile temperatures during most of the time they were tracked. The mean proportion of the time subyearlings tracked within the 16–20° C temperature range was larger than the proportion of time this range was available, which confirmed temperature selection opposed to random use. The subyearlings selected a depth and temperature combination that allowed them to increase their exposure to temperatures of 16–20° C when temperatures <16 and >20° C were available at lower and higher positions in the water column. A portion of the subyearlings that selected a temperature c. 17·0° C during the day, moved into warmer water at night coincident with an increase in downstream movement rate. Though subyearlings used temperatures outside of the 16–20° C range part of the time, behavioural thermoregulation probably reduced the effects of intermittent exposure to suboptimal temperatures. By doing so, it might enhance growth opportunity and life-history diversity in the population of subyearlings studied.     

Ceratium in Lake Erken: vertical distribution, migration and form variation

External morphological features and growth characteristics of the dinoflagellates Ceratium hirundinella and C. furcoides were investigated in relation to the environmental conditions in Lake Erken. Three distinct population maxima were distinguished, one in June, one in July, and one in September. C. hirundinella dominated the spring and summer populations. C. furcoides was rare in June, but it continuously increased and composed ca. 18% of the Ceratium-cetts in the autumn. The size of a C. hirundinella decreased during the investigation period and a form with a long horn disappeared in June. Ceratium was most numerous during the warmer periods in summer when a distinct thermocline appeared and before the turnover period in September. The alga preferred depths down to 3 m where the blue light dominated and the following parametric magnitudes could be measured: oxygen contents >5 mg 1^-1, pH >7, HCO₃, >1.7 meq. · 1^-1, and a specific conductivity of ca. 250 mS · cm^-1. The algal growth periods displayed doubling times of 2.63 and 4.04 days at surface temperatures of 19°C and 15°C, respectively. The water transparency decreased 3.8 m when Ceratium reached cell densities of 13 individuals · ml^-1 (0–10 m depth). Its vertical migration demonstrated a dial rhythm with accumulation of cells in the surface waters during the day. Complicated patterns of surface avoidance occurred in clear weather and under wind stress.     

The hygienic efficiency of conventional and inverted lamb dressing systems

R.G. BELL AND S.C. HATHAWAY. 1996. Aerobic plate counts (APC 37°C and APC 25°C) and Escherichia coli enumerations (Petrifilm) were used to determine sources of bacterial contamination during sheep dressing, determine the hygienic efficacy of hand wash and knife 'sterilization'procedures and compare the hygiene efficiency of conventional and inverted sheep dressing systems. The major slaughterline sources of microbial contamination were: fleece > workers' hands > faecal pellets > knife blades. Aerobic plate counts (APC 37°C) exceeding log 4.4 cfu cm^-2 were considered indicative of direct fleece contact, whereas E. coli numbers exceeding log 3.3 cfu cm^-2 were considered indicative of direct faecal contact. A 44°C water hand rinse removed 90% of the microbial contamination from workers' hands, but rinsed hands, particularly those contacting the fleece, still carried a microbial population exceeding log 4.0 cfu cm^-2. A 44°C rinse followed by an 82°C water dip reduced the contamination on knife blades to less than log 3.0 cfu cm^-2. Inverted dressing systems produced carcasses with a lower contamination level than conventional systems. With both systems little increase in contamination occurred after pelt removal. The areas of highest contamination were the forequarter region with inverted dressing and the hindquarter with conventional dressing. In both cases these regions are the sites where cuts are made through the skin. With both systems contamination around these cuts was entirely consistent with direct fleece contact resulting from 'rollback'.     

Light and pressure in two freshwater lakes and their influence on the growth, morphology and depth limits of Hippuris vulgaris

SUMMARY. Hippuris vulgaris was found growing down to a depth of 6 m in two clear-water lakes. Mean summer water temperatures of the epilimnia were 15–18°C. Midsummer photosynthetically available radiation, (PAR 400–700 nm) at 6 m was 100 μeinsteins m⁻²s⁻¹. In the laboratory, shoots of H. vulgaris continued to elongate at temperatures of 15 and 20°C and at pressures of 1.0, 1.8 and 2.3 atm. (corresponding to 0 m, 8.0 m and 13.3 m depths of water, respectively) providing PAR was kept above 100 °E m⁻²s⁻¹. Leaf primordia were initiated further from the apex in shoots grown under a pressure of 2.3 atm., but the site of initiation is not critical for the subsequent growth of leaves in H. vulgaris . Lacunae in shoots grown under a pressure of 2.3 atm. were also larger than those in control shoots, implying that pressure does not constrict air spaces. Root growth is not inhibited by a pressure of 2.3 atm. Field measurements and laboratory experiments indicate that in warm water, PAR is the environmental factor most likely to control the depth limits of H. vulgaris in the field.     

Changes in movement, range and habitat preferences of adult grayling from late summer to early winter

Radio‐tagged adult grayling Thymallus thymallus ( n  = 22), monitored from mid August to mid December 1999 in the River Kuusinkijoki, Finland, shifted by the end of September (water temperature 10·0–14·5° C) from riffle sites to deeper and slower pool sites 0·7–1·6 km up‐ or downstream. In early winter ( c . 0° C water temperature), eight of 13 fish still under study made a further shift into new pool sites, possibly triggered by ice formation. The summer range of grayling in the riffles was smaller (mean ±  s . d . length: 75 ± 146 m) than the autumn range (99 ± 46 m) in the pools, but gross daily movements were equally short in both the seasons (18 ± 34 m and 15 ± 7 m, respectively). In late summer, adult grayling preferred water depths 80–120 cm and mean velocities >40 cm s⁻¹. In autumn, the preferred ranges were 100–240 cm and <30 cm s⁻¹, respectively. Substratum was mainly boulders in the summer sites, and gravel or pebbles in the autumn sites.     

Temperature-related fluctuations in the timing of emergence and pupation of Windermere alder-flies over 30 years

1. From 1966 to 1995, dates were recorded when adult alder-flies, Sialis lutaria L., were first seen (30-year range: 23 April – 25 May), 50% of the maximum density occurred (4 May – 4 June), and maximum density occurred (11 May – 17 June) along 200 m of Windermere shore. These emergence dates occurred at similar temperatures, estimated by mean values for both the emergence date and the week prior to emergence. The latter was the least variable at 10.1 °C (95% CL ± 0.37) for start of emergence, 11.2 °C (± 0.49) for 50% maximum density, 14.2 °C (± 0.51) for maximum density.
2. Final-instar larvae pupated in damp soil just above the water line. As laboratory temperatures were increased slowly from an initial 5 °C, the cumulative number of larvae leaving the water to pupate increased. A quadratic equation described this relationship from a threshold temperature of 7.2 °C to completion at 14.0 °C (50% point, 9.3 °C). The relationship between successful pupations and constant temperatures in the laboratory was well described by a quadratic equation with an optimum 14.9 °C (over 90% success) and no success outside the range 7–23 °C. A negative power-function described the relationship between days required for pupation and temperature, ranging from c . 28 days at 8.2 °C to c . 4 days at 22.1 °C.
3. Dates for larvae leaving the lake to pupate were back-calculated from dates for adult emergence, using the power-function for pupation time. Mean temperatures for estimated dates on which larvae left the lake to pupate were less variable than those for adult emergence, being 7.5 °C (± 0.20) for the start of pupation, 9.4 °C (± 0.16) for 50% maximum density, 13.7 °C (± 0.16) for maximum density. These values are similar to those obtained in the laboratory and can be used to predict pupation and adult emergence for different temperature regimes.     

Short-Term Changes in Heat Tolerance in the Alpine Cushion Plant Silene acaulis ssp. excapa [All.] J. Braun at Different Altitudes

Abstract: The habit of cushion growth positively affects plant temperature but at the same may increase the risk of occasional overheating. In order to determine the adaptive response to short-term heat stress, we exposed S. acaulis cushions at field sites to controlled heat treatments using infrared lamps. Natural diurnal changes in heat tolerance were monitored at alpine sites and at a site distinctly below the natural distribution boundary, where higher temperatures were expected. The range of heat tolerance limits in summer, 45.5 - 54.5 °C (9 K), exceeded that reported for other alpine species (0.1 - 5 K) and even that for total seasonal changes (5 - 8 K). Heat tolerance either increased or decreased on most days (80 %). The maximum diurnal increase was + 4.7 K. Under the experimental conditions heat hardening started at leaf temperatures around 30 °C and proceeded at mean rates of 1.0 ± 0.5 K/h. The onset of functional disturbances in photosystem II also occurred at 30 °C. Heating rates exceeding those naturally found above 30 °C (> 10 K/h) appeared to retard heat hardening. During summer average leaf temperature maxima were 12.4 K (600 m) and 13.0 K (1945 m) higher than air temperature which corroborates the heat trapping nature of cushion plants. At 600 m, as compared to 1945 m, cushions experienced significantly higher leaf temperature maxima (+ 8.8 K) and exceeded 30 °C on most days (80 %). This resulted in a significantly higher heat tolerance (LT₅₀) at 600 m (51.7 ± 0.2 °C) than at 1945 m (49.8 ± 0.2 °C). The fast short-term changes of heat tolerance in summer help S. acaulis to cope with the occasional diurnal short-term heat stress associated with cushion growth.     

Laboratory studies of egg development and diapause in Isoperla obscura (Plecoptera) from a mountain stream in Norway

SUMMARY 1. A laboratory study of egg development of the stonefly Isoperla obscura (Zetterstedt) collected from the stream Flybekken (southern Norway, 61°25'N, 8°48'E, 1373 m a.s.l.) showed a short diapause followed by a prolonged period of postdiapause quiescence.
2. Diapause occurred over a wide range of temperatures (−20°C to +8°C), but 0–1°C was the most favourable for fast diapause development and successful hatching. Diapause development required temperatures below 12°C, but sub-zero diapause temperatures increased mortality during the postdiapause phase, as well as reducing hatching success.
3. The threshold for initiation of postdiapausal development was about 1–2°C. Development rate increased and variation in development time decreased at higher water temperatures. These may be important characteristics to ensure seasonal and geographical synchrony of development in individual populations. Low postdiapausal temperatures were unfavourable, despite a subsequent increase in water temperature. No response to postdiapausal photoperiod was detected.
4. The results agreed well with conditions noted in the natural environment, and with the abundance of this species at high altitudes and latitudes in Fennoscandia. Nevertheless, Isoperla obscura also occurs in streams in the maritime parts of western Norway. The results of the present study suggest that egg development in these populations will prove to be different.     

Primary and secondary induction requirements for flowering of Festuca rubra

Root and shoot temperatures were varied independently to determine the importance of root temperature during cold acclimation. Spinach ( Spinacia oleracea L. cvs Harbin and Bloomsdale) plants were subjected to 20/20°C. 20/5°C, 5/20°C, and 5/5°C (shoot/root) temperature treatments. Leaf freezing tolerance, water potential, stomatal resistance, osmotic potential, and water content were measured at 0.25. 1.25, 3.25, and 7.25 days of treatment. There was no change in freezing tolerance or the water relations of the 20/20°C treated plants during the course of the experiment. Freezing tolerance was increased by the 5°C shoot temperature treatments, but was not enhanced by water stress induced by the low root temperature. Leaf water potential and water content decreased and stomatal resistance increased within 6 h in the 20/5°C plants. By day 3, osmotic potential began decreasing in the 20/5°C plants. Leaf water content, osmotic potential, and water potential decreased more gradually in plants grown with 5°C shoot temperature, irrespective of root temperature. Decreased water content and osmotic potential were not correlated with increased freezing tolerance as reported for other herbaceous crop plants.     

Incubation of eggs of tuatara, Sphenodon punctatus

Eggs of the tuatara, Sphenodon punctatus , were incubated either buried or half buried in vermiculite at constant temperatures of 15, 18, 20, 22 and 25 °C and constant water potentials between —90 and —400 kPa. Many clutches failed completely, possibly because they had been taken from females prior to proper shell development. Failed eggs were significantly smaller than successful eggs. Incubation is unsuccessful at 15 °C. Hatching success is high between 18 and 22 °C but low at 25 °C, but equally successful between 18 and 22°C. Incubation is strongly influenced by temperature, with mean incubation periods of 328 days at 18 °C, 259 days at 20 °C, 169 days at 22 °C and 150 days at 25 °C. Water potential generally has little influence on incubation time at a given temperature. Buried eggs hatch sooner than partially buried eggs at 20 °C but the large range makes significance dubious.
Eggs on the driest substrata at 18 and 20 °C lose water initially but then gain water through the rest of incubation. Eggs in all other conditions gain water throughout incubation, with the rate of i water absorption being maintained or increasing late in incubation. The suggestion that increasing rate of water absorption late in incubation facilitates explosive hatching is not supported. Egg mass at the time of hatching varies from 132 to 398% of initial values, depending on incubation conditions. Final egg mass is not affected significantly by incubation temperature. Hence, rates of absorption increase with temperature.
Water potential has no influence on hatchling size. However, hatchlings from buried eggs generally are significantly larger than those from partially buried eggs.     

The effect of a self-regulating trace heating element on legionella within a shower

A self-regulating trace heating element was assessed for its ability to maintain a temperature of 50°C in the mixer valve and dead-legs of a shower, and for its effect on legionellas colonizing the shower. The trace heating element maintained a temperature of 50°C ± 1·5°C in dead-legs when the circulating hot water supply remained above 45°C. Legionellas appeared in a trace heated dead-leg when the temperature of the dead-leg reached 45°C and the hot water supply dropped below this temperature. Legionellas were eradicated or significantly reduced in sections of the shower where a temperature of 50°C was consistently achieved. The mixer valve which was trace heated but not insulated remained colonized with Legionellas. Legionellas were found in shower water throughout the study.     

Influence of temperature on life-table parameters of Stethorus gilvifrons (Mulsant) (Coleoptera: Coccinellidae) fed on Tetranychus urticae Koch

The influence of temperature on life-table parameters, fecundity and survivorship of the predatory ladybird, Stethorus gilvifrons , fed on Tetranychus urticae was determined at seven constant temperatures of 15°C, 20°C, 25°C, 28°C, 30°C, 35°C and 40°C. No development was observed at 40°C, thus being regarded as the threshold for the development of S. gilvifrons . The results indicate a significant decrease in male and female longevity with increasing temperature from 15°C to 35°C. The longest and shortest longevity were 18.40 and 12.75 days for males and 17.40 and 8.80 days for females, respectively. The intrinsic rate of natural increase ( r _m ) and the net reproductive rate ( R ₀) of S. gilvifrons linearly increased with increasing temperatures from 15°C to 35°C, while the mean generation time ( T ) and doubling time (DT) decreased linearly within this temperature range. The highest values of r _m (0.240 females/female/day) and R ₀ (59.27 females/female) and the lowest mean generation time (17.01 days) and DT (2.88 days) were recorded at 35°C. The maximum (185.50 eggs) and minimum (25.50 eggs) measurement of total fecundity was also recorded at 35°C and 15°C, respectively. The results indicate that temperature greatly affected fecundity, survivorship and life-table parameters of S. gilvifrons , and that 35°C is a suitable temperature for population growth of this predator.     

Rafting in Antarctic Collembola

Darwin was an early exponent of the importance of 'occasional means of dispersal' in accounting for the present-day distribution of plants and animals. This study examined the implications of capture on the water surface of meltwater and seawater for the local and long-range dispersal of Antarctic springtails. Individuals of the maritime Antarctic collembolan Cryptopygus antarcticus , were floated on tap water and seawater at 0, 5 and 10°C. LT₅₀s on seawater were 34 (10°C), 65 (5°C) and 75 (0°C) days. On tap water, LT₅₀s were 69 (10°C), 126 (5°C) and 239 (0°C) days. Less than 20% escaped from the water surface. A significantly greater proportion of springtails moulted on tap water and viable offspring were produced on both tap water and seawater. Comparison across treatments of survival of moulting and non-moulting individuals found significantly greater survival in moulting animals for three of the treatment combinations. It is suggested that moult exuviae facilitate survival on the water film through the simultaneous provision of a flotation aid and a source of nourishment – that is, an 'edible raft'. A separate experiment measuring changes in haemolymph osmolality over time on tap water and seawater at 2 and 5°C found significant differences in all treatments. Causes of mortality are discussed in relation to osmoregulatory failure and starvation.     

Melosira distans var. tenella and Eunotia zasuminensis, two poorly known planktonic diatoms in Finnish lakes

The occurrence and ecology of two poorly known planktonic diatoms, Melosira distans var. tenella (Nyg.) M.-B. Florin and Eunotia zasuminensis (Cabejsz.) Körner in central Finland is discussed. Both species have their maximum in late summer when water temperature is > 15°C and they prefer slightly acid water (pH < 7), low conductivity (less than 4 mS/m) and a moderate degree of dystrophy with C.O.D. 10–20 mg O₂/l and water colour 60–120 mg Pt/1. Melosira distans var. tenella was found in ca. 80% of the central Finnish lakes studied, but it is not reported in previously published papers. Eunotia zasuminensis is rarer, but is, however, recorded in at least 22 lakes in central Finland.     

Protein, lipid and caloric contents of bluntnose minnow, Pimephales notatus Rafinesque, during growth at different temperatures

Changes in the relative proportions of protein, lipid, water and caloric contents of bluntnose minnow growing at various temperatures (15, 25, 30° C) were investigated by application of the allometry equation, y=ax^b . Fish grew significantly faster at 25° C (closest to optimum), more slowly at 30° C and most slowly at 15° C. Protein, as a percentage of body wet weight, tended to increase with fish size at all temperatures ( b > 1.000), whereas in juveniles (<0.7 g) it decreased ( b < 1.000). However, with the exception of the 15° C group, protein as a percentage of body dry weight, decreased in all groups ( b < 1.000). Temperature appeared to modify the body composition of bluntnose minnows, e.g. decreasing temperature led to significantly enhanced protein content during growth. Lipid (%) and caloric content (cal g⁻¹) increased with increasing fish weight ( b > 1). The slower growing fish (15°, 30° C) deposited significantly more lipid (and had higher caloric contents) than those growing most rapidly (at 25° C). Water content (%) decreased with increasing body weight in all groups. Despite intergroup growth rate differences, all groups showed evidence of a tendency to follow similar trends in b values for body constituents and caloric content (except for protein v. body dry weight for the 15° C group). This suggests a general conservativeness of body composition in bluntnose minnow. The correlations between body constituents, caloric content and body weight were high ( r ²>0.9) so that estimates of body composition can be obtained from body weight for all temperature groups.