共查询到20条相似文献,搜索用时 31 毫秒
1.
K. H. Schneider 《Applied microbiology and biotechnology》1994,41(5):578-583
Mannitol dehydrogenase (MDH) from Rhodobacter sphaeroides Si4 was overproduced by constructing a strain that overexpresses the MDH gene and by producing high cell concentrations via fed-batch cultivation in a bioreactor. With the gene of mannitol dehydrogenase (mtlK) cloned into the expression vector pKK223-3 expression of MDH in Escherichia coli was obtained, but the specific enzyme activity was lower than in R. sphaeroides Si4. In order to overexpress mtlK in R. sphaeroides, plasmid pAK82 was constructed by cloning a DNA fragment carrying mtlK into the broad-host-range expression vector pRK415. When pAK82 was introduced into R. sphaeroides Si4 the specific mannitol dehydrogenase activity in the strain obtained was 0.48 unit (U)mg–1, 3.4-fold higher thain in the wild type. In this way the enzyme yield from cultivation in a bioreactor could be improved from 110 Ul–1 to 350 Ul–1. A further increase in productivity was obtained by fed-batch cultivation of R. sphaeroides Si4 [pAK82]. Using this cultivation method can optical density of 27.6 was reached in the bioreactor, corresponding to a dry mass of 16.6 g l–1. Since MDH formation correlated with biomass production, the MDH yield could be raised to 918 Ul–1, an 8.3-fold increase in comparison to batch cultivation of the wild-type strain.Dedicated to Prof. Fritz Wagner on the occasion of his 65th birthday. 相似文献
2.
5-Aminolevulinate production with recombinant <Emphasis Type="Italic">Escherichia coli</Emphasis> using a rare codon optimizer host strain 总被引:1,自引:0,他引:1
The 5-aminolevulinate (ALA) synthase gene (hemA) containing several codons rarely used by Escherichia coli was cloned from the genome of Rhodobacter sphaeroides and optimized in two strains of Escherichia coli: BL21(DE3) and Rosetta(DE3), which is a rare codon optimizer strain. The effects of initial isopropyl-β-d-thiogalactopyranoside (IPTG) concentration, induction time, and temperature on enzyme activity were studied and compared
for two strains. The results indicated that the ALA synthase expressed by Rosetta(DE3)/pET-28a(+)-hemA was higher than that by BL21(DE3)/pET-28a(+)-hemA. The initial precursors, glycine and succinate, and initial glucose, which is an inhibitor for both ALA synthase and dehydratase,
were observed to be the key factors affecting ALA production. ALA synthase activity was generally higher with Rosetta(DE3)
than with BL21(DE3), so was ALA biosynthesis. Based on the optimal culture system using Rosetta(DE3), the yield of ALA achieved
3.8 g/l (29 mM) under the appropriate conditions in fermenter. 相似文献
3.
C. J. Park J. H. Lee S.-S. Hong H.-S. Lee S. C. Kim 《Applied microbiology and biotechnology》1998,50(1):71-76
To produce a large quantity of the angiotensin-converting-enzyme(ACE)-inhibiting peptide YG-1, which consists of ten amino
acids derived from yeast glyceraldehyde-3-phosphate dehydrogenase, a high-level expression was explored with tandem multimers
of the YG-1 gene in Escherichia coli. The genes encoding YG-1 were tandemly multimerized to 9-mers, 18-mers and 27-mers, in which each of the repeating units
in the tandem multimers was connected to the neighboring genes by a DNA linker encoding Pro-Gly-Arg for the cleavage of multimers
by clostripain. The multimers were cloned into the expression vector pET-21b, and expressed in E. coli BL21(DE3) with isopropyl β-d-thiogalactopyranoside induction. The expressed multimeric peptides encoded by the 9-mer, 18-mer and 27-mer accumulated intracellularly
as inclusion bodies and comprised about 67%, 25% and 15% of the total proteins in E. coli respectively. The multimeric peptides expressed as inclusion bodies were cleaved with clostripain, and active monomers were
purified to homogeneity by reversed-phase high-performance liquid chromatography. In total, 105 mg pure recombinant YG-1 was
obtained from 1 l E. coli culture harboring pETYG9, which contained the 9-mer of the YG-1 gene. The recombinant YG-1 was identical to the natural YG-1
in molecular mass, amino acid sequence and ACE-inhibiting activity.
Received: 6 January 1998 / Received revision: 23 February 1998 / Accepted: 24 February 1998 相似文献
4.
The mercury transporter, merT, from Cupriavidus metallidurans was cloned into pRSET-C and expressed in various E. coli hosts. Expression of merT gene failed in common expression hosts like E. coli BL21(DE3), E. coli BL21(DE3)pLysS and E. coli GJ1158 due to expression induced toxicity. The protein was successfully expressed in E. coli C43(DE3) as inclusion bodies. The inclusion bodies were solubilized with Triton X-100 detergent. The detergent solubilized
protein with N-terminal His-tag was purified in a single-step by immobilized metal affinity chromatography with a yield of 8 mg l−1. 相似文献
5.
Deletion of both iclR and arcA in E. coli profoundly alters the central metabolic fluxes and decreases acetate excretion by 70%. In this study we investigate the metabolic
consequences of both deletions in E. coli BL21 (DE3). No significant differences in biomass yields, acetate yields, CO2 yields and metabolic fluxes could be observed between the wild type strain E. coli BL21 (DE3) and the double-knockout strain E. coli BL21 (DE3) ΔarcAΔiclR. This proves that arcA and iclR are poorly active in the BL21 wild type strain. Noteworthy, both strains co-assimilate glucose and acetate at high glucose
concentrations (10–15 g l−1), while this was never observed in K12 strains. This implies that catabolite repression is less intense in BL21 strains compared
to in E. coli K12. 相似文献
6.
Guerrero SA Lopez JA Steinert P Montemartini M Kalisz HM Colli W Singh M Alves MJ Flohé L 《Applied microbiology and biotechnology》2000,53(4):410-414
Tryparedoxin peroxidase has recently been identified as a constituent of the complex peroxidase system in the trypanosomatid
Crithidia fasciculata [Nogoceke E, Gommel DU, Kiess M, Kalisz HM, Flohé L (1997) Biol Chem 378: 827–836]. In trypanosomatids, hydroperoxides are
reduced at the expense of NADPH by means of a cascade of three oxidoreductases: the flavoprotein trypanothione reductase,
tryparedoxin and tryparedoxin peroxidase. Inhibitors of these enzymes are presumed to be trypanocidal drugs. Here, we present
the heterologous expression of a putative tryparedoxin peroxidase gene of Trypanosoma cruzi (accession no AJ012101) as an N-terminally His-tagged protein (TcH6TXNPx). The product was purified with a high yield (8.75 mg
from 1 l fermentation broth of A
600 2.1) from the cytosolic fraction of sonified Escherichia coli BL21(DE3)[pET22b(+)/TcH6TXNPx] by metal-chelating chromatography. TcH6TXNPx proved to be fully active when tested with heterologous
tryparedoxins of C. fasciculata (His-tagged TXN1H6 and TXN2H6). TcH6TXNPx displayed ping-pong kinetics with a k
cat of 1.7 s−1 and limiting K
m values of 51.8 μM and 1.7 μM for t-butyl hydroperoxide and CfTXN2H6, respectively.
Received: 3 September 1999 / Accepted: 1 November 1999 相似文献
7.
Artificial amplification of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) under glycolytic conditions enables Escherichia coli to maintain a greater intracellular ATP concentration during its growth phase. To demonstrate the biotechnological benefit
of E. coli harboring a high intracellular ATP concentration, we compared the recombinant protein synthesis of a soluble protein (enhanced
green fluorescence protein, GFP) with that of a secretory protein (alkaline protease, AP), under control of the T7 promoter
in E. coli BL21(DE3) overexpressing PCK. According to the batch fermentations, the strain overexpressing PCK produced more GFP and AP
with a lower increase in biomass than the control strain. In a chemostat culture (D = 0.7 h−1), the GFP production in the PCK overexpressing strain was 99.0 ± 4.31 mg/g cell, with a biomass of 0.22 g/L, while that of
the control strain was 53.5 ± 3.07 mg/g cell, with a biomass of 0.35 g/L. These results indicate that the PCK overexpressing
E. coli strain harboring high intracellular levels of ATP can be useful as a protein-synthesizing host. The potential uses of the
strain and associated rationale are discussed. 相似文献
8.
To improve the plasmid stability during the production of antihuman ovarian carcinoma × antihuman CD3 single-chain bispecific
antibody (AhOC×AhCD3), the Escherichia coli BL21(DE3) host cell was optimized serially. Firstly, an isogenic recombination-deficient (recA
−) derivative of BL21(DE3), namely BLR(DE3), was used as host instead of BL21(DE3). Although the segregational plasmid stability
was greatly improved, AhOC×AhCD3 yield was not improved due to the severe growth inhibition of plasmid-bearing BLR(DE3) cells
and the competitive plasmid instability after induction. Secondly, a mutant BLR(DE3), namely BLRM(DE3), was screened by using
LB agar plates plus ampicillin and isopropyl-β-d-thiogalactopyranoside. Using this new host, growth inhibition of recombinant cells after induction was eliminated, and plasmids
could be stably maintained even after long-time induction in a nonselective medium. At last, about 1.2 g/l AhOC×AhCD3, which
was about thrice as much as those of recombinant BL21(DE3) and BLR(DE3) strains, was yielded. 相似文献
9.
To understand how Mycobacterium tuberculosis (M. tuberculosis) could survive in human lung, Genomic expression library of M. tuberculosis in Escherichia coli (E. coli) had been prepared. Taking advantage of the genetic simplicity of E. coli and the functional conservation of some prokaryote proteins, a surfactant stress resistant gene Rv0621 was identified, which
encodes a 37 kDa putative membrane protein. The E. coli colony with the partial Rv0621 gene insert, named S1, was able to grow in medium containing 0.4% sodium dodecyl sulfate,
while the strain carried empty vector was unable to grow. The full length of the Rv0621 gene was then cloned into plasmid
pET32a (+) expressed in E. coli BL21 (DE3). Using gas chromatographic–mass spectrometric (GC–MS), the fatty acid composition of the E. coli BL21 (DE3) carrying Rv0621–pET32a (+) and the E. coli BL21 (DE3) carrying empty vector pET32a (+) were compared. E. coli BL21 (DE3) carrying Rv0621–pET32a (+) contained more oleic acid. This suggests the gene may be involved in regulation of
fatty acid synthesis and M. tuberculosis resistance to the surfactant defense of its host. 相似文献
10.
Zhang M Jiang X Su Z Lin J Xiang Q Yang Z Huang Y Li X 《Applied microbiology and biotechnology》2012,93(2):613-621
As a novel important regulator of glucose and lipid metabolism homeostasis, human fibroblast growth factor 21 (hFGF21) has
become a potential drug candidate for the treatment of metabolic diseases including obesity, and type 2 diabetes, as well
as non-alcoholic fatty liver disease. To improve the production of recombinant hFGF21 to meet the increasing demand in clinical
applications, an artificial gene encoding its mature peptide sequence was constructed, cloned into vector pET-3c and then
expressed in Escherichia coli Origami B (DE3). Under optimal conditions in a 50-L fermentor, the average bacterial yield and the soluble expression level
of recombinant hFGF21 of six batches attained 1750 ± 185 g and 32 ± 1.5%, respectively. The target protein was purified by
the combination of nickel-nitrilotriacetic acid affinity chromatography and Sephadex S-100 resin. 5% (w/v) trehalose solution was able to prevent rhFGF21 from degradation effectively. The purity of rhFGF21 was higher than 97%,
and the yield was 213 ± 17 mg/L. The preliminary biochemical characterization of rhFGF21 was confirmed using Western blot
and peptide map finger analysis. Based on the glucose oxidase–peroxidase assay, the EC50 of glucose uptake activity of the
purified rhFGF21 was 22.1 nM. 相似文献
11.
The isoeugenol monooxygenase gene of Pseudomonas putida IE27 was inserted into an expression vector, pET21a, under the control of the T7 promoter. The recombinant plasmid was introduced
into Escherichia coli BL21(DE3) cells, containing no vanillin-degrading activity. The transformed E. coli BL21(DE3) cells produced 28.3 g vanillin/l from 230 mM isoeugenol, with a molar conversion yield of 81% at 20°C after 6 h.
In the reaction system, no accumulation of undesired by-products, such as vanillic acid or acetaldehyde, was observed. 相似文献
12.
Pengjun Shi Guoyu Yao Peilong Yang Ning Li Huiying Luo Yingguo Bai Yaru Wang Bin Yao 《Applied microbiology and biotechnology》2010,85(5):1483-1490
An endo-1,3-β-d-glucanase gene, designated as bglS27, was cloned from Streptomyces sp. S27 and successfully expressed in Escherichia coli BL21 (DE3). The full-length gene contains 1,362 bp and encodes a protein of 453 amino acids with a calculated molecular mass
of 42.7 kDa. The encoded protein comprises a catalytic module of glycosyl hydrolase family 16, a short glycine linker region,
and a family 13 carbohydrate-binding module. The purified recombinant enzyme (BglS27) showed optimal activity at 65°C and
pH 5.5 and preferentially catalyzed the hydrolysis of glucans with a β-1,3-linkage using an endolytic mode of action. The
specific activity and K
m value of BglS27 for laminarin were 236.0 U mg–1 and 1.89 mg ml–1, respectively. In antifungal assay, BglS27 had the ability to inhibit the growth of phytopathogenic fungi Rhizoctonic solani and Fusarium oxysporum and some mycotoxin-producing fungi Fusarium crookwellense and Paecilomyces variotii. These favorable properties make BglS27 a good candidate for utilization in biotechnological applications such as plant protection,
feed, and food preservation. 相似文献
13.
Byun SG Kim MD Lee WH Lee KJ Han NS Seo JH 《Applied microbiology and biotechnology》2007,74(4):768-775
A recombinant Escherichia coli strain was developed to produce guanosine 5′-diphosphate (GDP)-l-fucose, donor of l-fucose, which is an essential substrate for the synthesis of fucosyloligosaccharides. GDP-d-mannose-4, 6-dehydratase (GMD) and GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase (WcaG), the two crucial enzymes for
the de novo GDP-l-fucose biosynthesis, were overexpressed in recombinant E. coli by constructing inducible overexpression vectors. Optimum expression conditions for GMD and WcaG in recombinant E. coli BL21(DE3) were 25°C and 0.1 mM isopropyl-β-d-thioglucopyranoside. Maximum GDP-l-fucose concentration of 38.9 ± 0.6 mg l−1 was obtained in a glucose-limited fed-batch cultivation, and it was enhanced further by co-expression of NADPH-regenerating
glucose-6-phosphate dehydrogenase encoded by the zwf gene to achieve 55.2 ± 0.5 mg l−1 GDP-l-fucose under the same cultivation condition. 相似文献
14.
Hee-Jung Moon Manish Tiwari Marimuthu Jeya Jung-Kul Lee 《Applied microbiology and biotechnology》2010,87(1):205-214
Ribitol dehydrogenase (RDH) catalyzes the conversion of ribitol to d-ribulose. A novel RDH gene was cloned from Zymomonas mobilis subsp. mobilis ZM4 and overexpressed in Escherichia coli BL21(DE3). DNA sequence analysis revealed an open reading frame of 795 bp, capable of encoding a polypeptide of 266 amino
acid residues with a calculated molecular mass of 28,426 Da. The gene was overexpressed in E. coli BL21(DE3) and the protein was purified as an active soluble form using glutathione S-transferase affinity chromatography. The molecular mass of the purified enzyme was estimated to be ∼28 kDa by sodium dodecyl
sulfate-polyacrylamide gel and ∼58 KDa with gel filtration chromatography, suggesting that the enzyme is a homodimer. The
enzyme had an optimal pH and temperature of 9.5 and 65°C, respectively. Unlike previously characterized RDHs, Z. mobilis RDH (ZmRDH) showed an unusual dual coenzyme specificity, with a k
cat of 4.83 s−1 for NADH (k
cat/K
m = 27.3 s−1 mM−1) and k
cat of 2.79 s−1 for NADPH (k
cat/K
m = 10.8 s−1 mM−1). Homology modeling and docking studies of NAD+ and NADP+ into the active site of ZmRDH shed light on the dual coenzyme specificity of ZmRDH. 相似文献
15.
M. L. Rúa H. Atomi C. Schmidt-Dannert R. D. Schmid 《Applied microbiology and biotechnology》1998,49(4):405-410
An efficient expression system for the previously only weakly expressed thermophilic lipase BTL2 (Bacillus thermocatenulatus lipase 2) was developed for the production of large amounts of lipase in Escherichia coli. Therefore, the gene was subcloned in the pCYTEXP1 (pT1) expression vector downstream of the temperature-inducible λ promoter
PL. Three different expression vectors were constructed: (i) pT1-BTL2 containing the mature lipase gene, (ii) pT1-preBTL2 containing the prelipase gene and (iii) pT1-OmpABTL2 containing the mature lipase gene fused to the signal peptide of the OmpA protein, the major outer membrane
protein of E. coli. With pT1-BTL2 and pT1-preBTL2, comparable expression levels of 7000–9000 U/g cells were obtained independently of the E. coli host. In contrast, with E. coli JM105 harbouring pT1-OmpABTL2, 660 000 soluble lipase U/g cells was produced, whereas, with E. coli DH5α and BL321, production levels of 30 000 U/g cells were achieved. However, most of the lipase remained insoluble but active
after cell breakage because of the unprocessed OmpA signal peptide. A simple cholate extraction followed by proteinase K cleavage
and ultrafiltration allowed the isolation of 1.15 × 106 units of 90% pure mature lipase/wet cells.
Received: 29 August 1997 / Received revision: 17 November 1997 / Accepted: 18 November 1997 相似文献
16.
B. Jongsareejit R. N. Z. A. Rahman S. Fujiwara T. Imanaka 《Molecular & general genetics : MGG》1997,254(6):635-642
The gltA gene encoding a glutamate synthase (GOGAT) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 was cloned as a 6.6 kb HindIII-BamHI fragment. Sequence analysis indicates that gltA encodes a 481- amino acid protein (53 269 Da). The deduced amino acid sequence of KOD1-GltA includes conserved regions that
are found in the small subunits of bacterial GOGAT: two cysteine clusters, an adenylate-binding consensus sequence and an
FAD-binding consensus sequence. However, no sequences homologous to the large subunit of bacterial GOGAT were found in the
upstream or downstream regions. In order to examine whether GltA alone can act as a functional GOGAT, GltA was overexpressed
in Escherichia coli BL21 (DE3) cells using an expression plasmid. GltA was purified to homogeneity and shown to be functional as a homotetramer
of approximately 205 kDa, which is equivalent to the molecular weight of the native GOGAT from KOD1, thus indicating that
KOD1-GOGAT is the smallest known active GOGAT. GltA is capable of both glutamine-dependent and ammonia-dependent synthesis
of glutamate. Synthesis of glutamate by KOD1-GltA required NADPH, indicating that this enzyme is an NADPH-GOGAT (EC 1.4.1.13).
The optimum pH for both activities was 6.5. However, GltA exhibited different optimum temperatures for activity depending
on the reaction assayed (glutamine-dependent reaction, 80° C; ammonia-dependent reaction, 90° C).
Received: 30 October 1996 / Accepted: 13 January 1997 相似文献
17.
O. Pines S. Shemesh E. Battat I. Goldberg 《Applied microbiology and biotechnology》1997,48(2):248-255
Saccharomyces cerevisiae accumulates l-malic acid through a cytosolic pathway starting from pyruvic acid and involving the enzymes pyruvate carboxylase and malate
dehydrogenase. In the present study, the role of malate dehydrogenase in the cytosolic pathway was studied. Overexpression
of cytosolic malate dehydrogenase (MDH2) under either the strong inducible GAL10 or the constitutive PGK promoter causes a
6- to 16-fold increase in cytosolic MDH activity in growth and production media and up to 3.7-fold increase in l-malic acid accumulation in the production medium. The high apparent K
m of MDH2 for l-malic acid (11.8 mM) indicates a low affinity of the enzyme for this acid, which is consistent with the cytosolic function
of the enzyme and differs from the previously published K
m of the mitochondrial enzyme (MDH1, 0.28 mM). Under conditions of MDH2 overexpression, pyruvate carboxylase appears to be
a limiting factor, thus providing a system for further metabolic engineering of l-malic acid production. The overexpression of MDH2 activity also causes an elevation in the accumulation of fumaric acid and
citric acid. Accumulation of fumaric acid is presumably caused by high intracellular l-malic acid concentrations and the activity of the cytosolic fumarase. The accumulation of citric acid may suggest the intriguing
possibility that cytosolic l-malic acid is a direct precursor of citric acid in yeast.
Received: 22 January 1997 / Received revision: 14 April 1997 / Accepted: 19 April 1997 相似文献
18.
To efficiently produce xylobiose from xylan, high-level expression of an α-l-arabinofuranosidase gene from Thermotoga maritima was carried out in Escherichia coli. A 1.5-kb DNA fragment, coding for an α-l-arabinofuranosidase of T. maritima, was inserted into plasmid pET-20b without the pelB signal sequence leader, and produced pET-20b-araA1 with 8 nt spacing between ATG and Shine–Dalgarno sequence. A maximum activity of 12 U mg−1 was obtained from cellular extract of E. coli BL21-CodonPlus (DE3)-RIL harboring pET-20b-araA1. The over-expressed α-l-arabinofuranosidase was purified 13-fold with a 94% yield from the cellular extract of E. coli by a simple heat treatment. Production of xylooligosaccharides from corncob xylan by endoxylanase and α-l-arabinofuranosidase was examined by TLC and HPLC: xylobiose was the major product from xylan at 90 °C and its proportion
in the xylan hydrolyzates increased with the reaction time. Hydrolysis with in the xylanase absence of α-l-arabinofuranosidase gave only half this yield.
Revisions requested 27 October 2005; Revisions received 5 September 2005 相似文献
19.
Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmids 总被引:1,自引:0,他引:1
Carotenoids are of great commercial interest and attempts are made to produce different carotenoids in transgenic bacteria
and yeasts. Development of appropriate systems and optimization of carotenoid yield involves transformation with several new
genes on suitable plasmids. Therefore, the non-carotenogenic bacterium Escherichia coli JM101 was transformed in our study with several genes that mediated the biosynthetic production of the carotenoid zeaxanthin
in this host. Selection of plasmids for the introduction of five essential genes for zeaxanthin formation showed that a pACYC-derived
plasmid was the best. Multiplasmid transformation generally decreased production of zeaxanthin. By cotransformation with different
plasmids, limitations in the biosynthetic pathway were found at the level of geranylgeranyl-pyrophosphate synthase and β-carotene
hydroxylase. In our study a maximum zeaxanthin content of 289 μg/g dry weight was obtained. This involved the construction
of a plasmid that mediated high-level expression of β-carotene hydroxylase. The level of expression was demonstrated on protein
gels and solubilization by the mild detergent Brij 78 revealed that a significant portion of the expressed enzyme is located
in the E. coli membranes where it can exert its catalytic function. Based on the results obtained, new strategies for vector construction
and strain selection were proposed which could increase the present concentrations drastically. Optimal growth conditions
of the transfomed E. coli strains for carotenoid formation were found at a temperature of 28 °C and a cultivation period of 2 days.
Received: 28 November 1996 / Received revision: 24 March 1997 / Accepted: 27 April 1997 相似文献
20.
Hendrik Schewe Dirk Holtmann Jens Schrader 《Applied microbiology and biotechnology》2009,83(5):849-857
A recombinant Escherichia coli BL21 (DE3) strain overexpressing a variant of P450BM-3 (V26T/R47F/A74G/F87V/L188K; abbreviated: BL21 (P450BM-3 QM)) oxyfunctionalizes the bicyclic monoterpene α-pinene to α-pinene oxide, verbenol, and myrtenol. To address the low water
solubility and the toxicity of terpenoids, an aqueous–organic two-phase bioprocess was developed. Diisononyl phthalate was
selected as a biocompatible organic carrier solvent capable of masking the toxic effects mediated by α-pinene and of efficiently
extracting the products enabling scale-up to the bioreactor. With an aqueous to organic phase ratio of 3:2 and 30% (v/v) of α-pinene in the organic phase, a biocatalytic product formation period of more than 4 h was achieved. A comparison of
the biotransformation performance of BL21 (P450BM-3 QM) and a strain with an additional heterologous NADPH regeneration system comprising glucose facilitator and dehydrogenase,
but only expressing half the amount of P450BM-3 QM, shows comparable product concentrations of 1,020 ± 144 and 800 ± 61 mg lAq−1, respectively. The total product yields Y
P/P450 (μmol μmolP450−1) were 80% higher when the strain with the cofactor regeneration system was used. A total product concentration of over 1 g
lAq−1, corresponding to the highest value reported for microbial α-pinene oxyfunctionalization so far, marks a promising step forward
toward a future application of recombinant microorganisms for the selective oxidation of terpenoids to value-added products. 相似文献