共查询到20条相似文献,搜索用时 15 毫秒
1.
A. Cadiere B. Couturaud J. Boismard P. Le Cann A. Gérard A. Mas C. Faye L. Garrelly B. Roig 《Journal of applied microbiology》2013,115(1):290-297
Aims
Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly‐l ‐lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real‐time PCR quantification.Methods and Results
This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0·99 ± 0·04 and Y intercept when x = ?0·02 ± 0·28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16 600‐fold 1 l of sample and to detect and quantify down to 750 GC l?1 and 7500 GC l?1, respectively).Conclusions
To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water.Significance and Impact of the Study
This study gives valuable advance in the methods of concentration and diagnosis of virus in water. 相似文献2.
Elke EM Brouwers Alwin DR Huitema Jos H Beijnen Jan HM Schellens 《BMC clinical pharmacology》2008,8(1):7
Background
The aim of this study was to evaluate long-term platinum retention in patients treated with cisplatin and oxaliplatin. 相似文献3.
Karen F Chambers Joanna F Pearson Davide Pellacani Naveed Aziz Miodrag Gužvić Christoph A Klein Shona H Lang 《Journal of biomedical science》2011,18(1):45
Background
Stromal signalling increases the lateral cell adhesions of prostate epithelial cells grown in 3D culture. The aim of this study was to use microarray analysis to identify significant epithelial signalling pathways and genes in this process. 相似文献4.
Marco Camanni Luca Bonino Elena Maria Delpiano Paola Berchialla Giuseppe Migliaretti Alberto Revelli Francesco Deltetto 《Reproductive biology and endocrinology : RB&E》2009,7(1):109-7
Background
this study aims to evaluate the effectiveness and safety of laparoscopic conservative management of ureteral endometriosis. 相似文献5.
Garcia-Campayo J Magdalena J Magallón R Fernández-García E Salas M Andrés E 《Arthritis research & therapy》2008,10(4):R81
Introduction
The aim of this paper was to compare the efficacy of the treatments for fibromyalgia currently available in both primary care and specialised settings. 相似文献6.
Background
In this paper a novel method for prostate segmentation in transrectal ultrasound images is presented. 相似文献7.
Ueli Braun Charlotte Schnetzler Matteo Previtali Christian Gerspach Tanja Schmid 《BMC veterinary research》2011,7(1):2
Background
The aim of this report was to describe duodenal obstruction caused by a rubber foreign body in a cow. 相似文献8.
Y.‐H. Hsu S.R. Cook T.W. Alexander C.L. Klima Y.D. Niu L.B. Selinger T.A. McAllister 《Journal of applied microbiology》2013,114(6):1592-1603
Aims
This study aimed to characterize the impact of lytic and temperate bacteriophages on the genetic and phenotypic diversity of Mannheimia haemolytica from feedlot cattle.Methods and Results
Strictly lytic phages were not detected from bovine nasopharyngeal (n = 689) or water trough (n = 30) samples, but Myoviridae‐ or Siphoviridae‐like phages were induced from 54 of 72 M. haemolytica strains by mitomycin C, occasionally from the same strain. Phages with similar restriction fragment length polymorphism profiles (RFLP ≥70% relatedness) shared common host serotypes 1 or 2 (P < 0·000 1). Likewise, phages with similar RFLP tended to occur in genetically related host bacteria (70–79% similarity). Host range assays showed that seven phages from host serotypes 1, 2 and 6 lysed representative strains of serotypes 1, 2 or 8. The genome of vB_MhM_1152AP from serotype 6 was found to be collinear with P2‐like phage φMhaA1‐PHL101.Conclusions
Prophages are a significant component of the genome of M. haemolytica and contribute significantly to host diversity. Further characterization of the role of prophage in virulence and persistence of M. haemolytica in cattle could provide insight into approaches to control this potential respiratory pathogen.Significance and Impact of the Study
This study demonstrated that prophages are widespread within the genome of M. haemolytica isolates and emphasized the challenge of isolating lytic phage as a therapeutic against this pathogen. 相似文献9.
Andrés Cerezo L Mann H Pecha O Pleštilová L Pavelka K Vencovský J Senolt L 《Arthritis research & therapy》2011,13(4):R122
Introduction
The aim of this study was to examine the serum levels of S100 proteins and to evaluate their role in patients with recent-onset rheumatoid arthritis (RA). 相似文献10.
Jia-Lin Wang Xiao-Juan Jiang Qian Wang Li-Jing Hou Da-Wei Xu Jin-Xing Wang Xiao-Fan Zhao 《BMC developmental biology》2007,7(1):76
Background
The midgut undergoes histolysis and remodeling during the larval to adult transition in holometabolous insects, but the molecular mechanisms underlying this process are not well understood. 相似文献11.
Background
The purpose of this study was to investigate the coordination strategy of maximal-effort horizontal jumping in comparison with vertical jumping, using the methodology of computer simulation. 相似文献12.
Misaki K Morinobu A Saegusa J Kasagi S Fujita M Miyamoto Y Matsuki F Kumagai S 《Arthritis research & therapy》2011,13(3):R77
Introduction
The purpose of this study was to elucidate the effects of histone deacetylase inhibition on the phenotype and function of dendritic cells and on arthritis in SKG mice. 相似文献13.
Steven D Fleming Asgerally T Fazleabas Stephen C Bell 《Reproductive biology and endocrinology : RB&E》2008,6(1):6
Background
The purpose of this study was to examine the effect of insulin on expression and synthesis of IGFBP-1 and IGFBP-2 in the baboon endometrium in vitro. 相似文献14.
Erin Henry Luca Santini Mark A. J. Huijbregts Ana Benítez-López 《Global Ecology and Biogeography》2023,32(2):267-280
Aim
Whether intraspecific spatial patterns in body size are generalizable across species remains contentious, as well as the mechanisms underlying these patterns. Here we test several hypotheses explaining within-species body size variation in terrestrial vertebrates including the heat balance, seasonality, resource availability and water conservation hypotheses for ectotherms, and the heat conservation, heat dissipation, starvation resistance and resource availability hypotheses for endotherms.Location
Global.Time period
1970–2016.Major taxa studied
Amphibians, reptiles, birds and mammals.Methods
We collected 235,905 body size records for 2,229 species (amphibians = 36; reptiles = 81; birds = 1,545; mammals = 567) and performed a phylogenetic meta-analysis of intraspecific correlations between body size and environmental variables. We further tested whether correlations differ between migratory and non-migratory bird and mammal species, and between thermoregulating and thermoconforming ectotherms.Results
For bird species, smaller intraspecific body size was associated with higher mean and maximum temperatures and lower resource seasonality. Size–environment relationships followed a similar pattern in resident and migratory birds, but the effect of resource availability on body size was slightly positive only for non-migratory birds. For mammals, we found that intraspecific body size was smaller with lower resource availability and seasonality, with this pattern being more evident in sedentary than migratory species. No clear size–environment relationships were found for reptiles and amphibians.Main conclusions
Within-species body size variation across endotherms is explained by disparate underlying mechanisms for birds and mammals. Heat conservation (Bergmann's rule) and heat dissipation are the dominant processes explaining biogeographic intraspecific body size variation in birds, whereas in mammals, body size clines are mostly explained by the starvation resistance and resource availability hypotheses. Our findings contribute to a better understanding of the mechanisms behind species adaptations to the environment across their geographic distributions. 相似文献15.
Background
Ethnic differences have been reported with regard to several medical therapies. The aim of this study was to investigate the relation between ethnicity and thrombolysis in stroke patients. 相似文献16.
Introduction
The objective of this study was to investigate the effects of moderate-to-high intensity Nordic walking (NW) on functional capacity and pain in fibromyalgia (FM). 相似文献17.
Yi Tang Bo Wen Ying-ying Wu Tao Yu Wen-tao Wang Yong-yue Wang 《Central European Journal of Biology》2012,7(1):54-62
Background
The aim of this study was to investigate the effect of nanocrystalline hydroxyapatite (HA) coatings on the activity of osteoblasts. 相似文献18.
Objective
The aim of this study was to compare a theoretical neural net model with MEG data from epileptic patients and normal individuals. 相似文献19.
Hashimoto T Matsumoto MM Li JF Lawton MT Young WL;University of California San Francisco BAVM Study Group 《BMC neurology》2005,5(1):1
Background
The primary aim of this study is to demonstrate the feasibility of utilizing doxycycline to suppress matrix metalloproteinase-9 (MMP-9) in brain arteriovenous malformations (AVMs). 相似文献20.
Superoxide dismutase from Helicobacter pylori suppresses the production of pro‐inflammatory cytokines during in vivo infection
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