Recruitment of UBPY and ESCRT Exchange Drive HD-PTP-Dependent Sorting of EGFR to the MVB

1. Download : Download high-res image (201KB)
2. Download : Download full-size image

Highlights? HD-PTP is essential for sorting EGFR to the MVB lumen ? HD-PTP binds the ESCRT-0 subunit STAM2 at two sites ? CHMP4B and UBPY displace EGFR from ESCRT-0 and drive ESCRT-III association ? UBPY is important for sorting EGFR to the MVB lumen     

Protein model discrimination using mutational sensitivity derived from deep sequencing

1. Download : Download high-res image (318KB)
2. Download : Download full-size image

Highlights? Individual mutant phenotypes in a saturation library derived via deep sequencing ? Residue depth and active-site residue derivation from mutant phenotypes ? Mutant-phenotype-derived parameters used for protein model discrimination ? Derivation of sequence-structure-function relationships without protein isolation     

SIP1 Mediates Cell-Fate Decisions between Neuroectoderm and Mesendoderm in Human Pluripotent Stem Cells

1. Download : Download high-res image (184KB)
2. Download : Download full-size image

Highlights? Smad-interacting protein 1 (SIP1) regulates hESC differentiation ? SIP1 upregulation promotes neuroectodermal differentiation ? SIP1 inhibits mesendodermal and endodermal differentiation ? SMAD2/3 and NANOG/OCT4/SOX2 cooperatively regulate SIP1 expression     

Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation

1. Download : Download high-res image (232KB)
2. Download : Download full-size image

Highlights? The crystal structure of the aa:CP ligase in complex with carrier protein was solved ? The interaction relies on the idiosyncratic α-helix of the aa:CP ligase ? The complex represents a unique mode of carrier protein recognition and binding ? The mechanism of amino acid attachment to carrier protein is discussed     

Design of a Phosphorylatable PDZ Domain with Peptide-Specific Affinity Changes

1. Download : Download high-res image (234KB)
2. Download : Download full-size image

Highlights? Successful design of phosphorylation sites into a globular protein ? Redesigned PDZ domain phosphorylation is associated with local destabilization ? Phosphorylation switches peptide binding function ? Phosphorylation switches peptide recognition specificity     

The WW Domain Protein Kibra Acts Upstream of Hippo in Drosophila

1. Download : Download high-res image (238KB)
2. Download : Download full-size image

Highlights? The WW domain protein Kibra is a Hippo signalling component ? Kibra restricts organ size via Yorkie and acts upstream of Hippo and Merlin ? Kibra synergizes with Expanded and binds to Merlin     

Finding Protein Targets for Small Biologically Relevant Ligands across Fold Space Using Inverse Ligand Binding Predictions

1. Download : Download high-res image (269KB)
2. Download : Download full-size image

Highlights? Inverse ligand binding prediction is implemented with modern binding site predictors ? Use of just one protein-ligand complex allows accurate identification of other protein targets ? Inverse prediction is effective in finding structurally distant protein targets ? Consensus approach provides improvements     

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain

1. Download : Download high-res image (337KB)
2. Download : Download full-size image

Highlights? ESCRT-I subunit UBAP1 is essential for degradation of antiviral protein tetherin ? UBAP1 has a domain consisting of a solenoid of overlapping UBAs (SOUBA) ? Each of the three UBAs in the SOUBA binds monoubiquitin     

Microtubules Enable the Planar Cell Polarity of Airway Cilia

1. Download : Download high-res image (355KB)
2. Download : Download full-size image

Highlights? PCP proteins are asymmetric at apical junctions in the airways before ciliogenesis ? PCP protein asymmetry and cilium orientation emerge gradually during development ? Cilium misorientation correlates with loss of PCP protein asymmetry in PCP mutants ? Microtubules appear to organize cilium orientation and PCP protein asymmetry     

A Network Model of the Molecular Organization of Chromatin in Drosophila

1. Download : Download high-res image (434KB)
2. Download : Download full-size image

Highlights? DamID identifies 42 previously unknown chromatin proteins ? Binding maps of new and known chromatin components yield a chromatin network model ? The network model predicts functions of most of the 42 new chromatin proteins ? The chromatin protein network is functionally compartmentalized     

DOLORS: Versatile Strategy for Internal Labeling and Domain Localization in Electron Microscopy

1. Download : Download high-res image (249KB)
2. Download : Download full-size image

Highlights? DOLORS is a strategy for EM labeling using AviTag and streptavidin ? Internal domains within a large protein can be labeled and identified ? Higher accuracy and precision are achieved by sampling multiple RCT reconstructions ? Offers a powerful method for deciphering moderate-resolution EM structures     

A Matrix Protein Silences Transposons and Repeats through Interaction with Retinoblastoma-Associated Proteins

1. Download : Download high-res image (133KB)
2. Download : Download full-size image

Highlights? The Arabidopsis matrix protein TEK silences transposons and repeat-containing genes ? Binding of TEK on targets affects chromatin conformation and histone modifications ? TEK protein associates with FVE/MSI5-containing histone deacetylation complex ? TEK directs repressive modification as a key structural component in gene silencing     

Zinc Drives a Tertiary Fold in the Prion Protein with Familial Disease Mutation Sites at the Interface

1. Download : Download high-res image (72KB)
2. Download : Download full-size image

Highlights? Zinc binds to the prion protein octarepeat domain ? The zinc-bound octarepeat makes a tertiary contact to C-terminal helices 2 and 3 ? The encompassed surface carries a majority of the mutations in familial prion disease ? Familial mutations weaken this zinc-driven tertiary contact     

Efficient Retrograde Transport of Pseudorabies Virus within Neurons Requires Local Protein Synthesis in Axons

1. Download : Download high-res image (197KB)
2. Download : Download full-size image

Highlights? Pseudorabies virus (PRV) infection stimulates local protein synthesis in axons ? Infection induces the synthesis of LIS1, peripherin, and annexin A2 in axons ? Axonal inhibition of protein synthesis retards retrograde PRV capsid transport ? Prior damage in axons significantly reduces retrograde PRV capsid transport     

Redox-Dependent Control of FOXO/DAF-16 by Transportin-1

1. Download : Download high-res image (206KB)
2. Download : Download full-size image

Highlights? FOXO forms redox-sensitive, disulfide-dependent complexes with several proteins ? Transportin-1 binds to FOXO via a disulfide and regulates its nuclear localization ? Redox and insulin signaling govern FOXO nuclear localization via distinct pathways ? Redox control of longevity protein FOXO/DAF-16 is evolutionarily conserved     

Malaria Parasites Co-opt Human Factor H to Prevent Complement-Mediated Lysis in the Mosquito Midgut

1. Download : Download high-res image (182KB)
2. Download : Download full-size image

Highlights? Malaria gametes evade human complement by binding factor H ? The plasmodial protein PfGAP50 binds to factor H on the gamete surface ? Binding of factor H results in inactivation of the complement protein C3b ? Functional inhibition of factor H or PfGAP50 reduces parasite transmission to the mosquito     

Rapid Inactivation of Proteins by Rapamycin-Induced Rerouting to Mitochondria

1. Download : Download high-res image (355KB)
2. Download : Download full-size image

Highlights? We have developed a way of getting rid of proteins quickly (i.e., minutes not days) ? This approach provides new insights into the function of the coat protein AP-1     

Multiple Epigenetic Modifiers Induce Aggressive Viral Extinction in Extraembryonic Endoderm Stem Cells

1. Download : Download high-res image (699KB)
2. Download : Download full-size image

Highlights? Mouse ESCs, TSCs, and XEN cells have different capacities to suppress retroviral activity ? RNAi screens identified epigenetic factors that mediate silencing of retroviruses ? Candidates modulate integrated, as well as endogenous, retroviral element silencing ? An Argonaute protein binds integrated provirus and may mediate retroviral extinction