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1.
Measurements of gut contents and changes in the volume and colour of bile in the gall bladder of juvenile Atlantic salmon are described for a variety of feeding regimes. The gall bladders of actively feeding fish are virtually empty of bile. When deprived of food, the weight of the gall bladder increases within 6 h and reaches a value equivalent to approximately 20% of the liver weight within 36 h. Bile is pale straw, green and blue in colour after 1, 4 and 6 days of starvation respectively. Stored bile is released from the gall bladder in response to food entering the anterior hind gut. 相似文献
2.
Solomon MB Foster MT Bartness TJ Huhman KL 《American journal of physiology. Regulatory, integrative and comparative physiology》2007,292(1):R283-R290
Obesity is a world-wide epidemic, and many factors, including stress, have been linked to this growing trend. After social stress (i.e., defeat), subordinate laboratory rats and most laboratory mice become hypophagic and, subsequently, lose body mass; the opposite is true of subordinate Syrian hamsters. After social defeat, Syrian hamsters become hyperphagic and gain body mass compared with nonstressed controls. It is unknown whether this increase in body mass and food intake is limited to subordinate hamsters. In experiment 1, we asked, do dominant hamsters increase food intake, body mass, and adiposity after an agonistic encounter? Subordinate hamsters increased food intake and body mass compared with nonstressed controls. Although there was no difference in food intake or absolute body mass between dominant and nonstressed control animals, cumulative body mass gain was significantly higher in dominant than in nonstressed control animals. Total carcass lipid and white adipose tissue (WAT) (i.e., retroperitoneal and epididymal WAT) masses were significantly increased in subordinate, but not dominant, hamsters compared with nonstressed controls. In experiment 2, we asked, does footshock stress increase food intake, body mass, and adiposity. Hamsters exposed to defeat, but not footshock stress, increased food intake relative to nonstressed controls. In animals exposed to defeat or footshock stress, body mass, as well as mesenteric WAT mass, increased compared with nonstressed controls. Collectively, these data demonstrate that social and nonsocial stressors increase body and lipid mass in male hamsters, suggesting that this species may prove useful for studying the physiology of stress-induced obesity in some humans. 相似文献
3.
Social interactions in small groups of juvenile rainbow trout(Oncorhynchus mykiss) lead to the formation of dominance hierarchies.Dominant fish hold better positions in the environment, gaina larger share of the available food and exhibit aggressiontowards fish lower in the hierarchy. By contrast, subordinatefish exhibit behavioural inhibition, including reduced activityand feeding. The behavioural characteristics associated withsocial status are likely the result of changes in brain monoaminesresulting from social interactions. Whereas substantial physiologicalbenefits, including higher growth rates and condition factor,are experienced by dominant trout, low social status appearsto be a chronic stress, as indicated by sustained elevationof circulating cortisol concentrations in subordinate fish.High cortisol levels, in turn, may be responsible for many ofthe deleterious physiological consequences of low social status,including lower growth rates and condition factor, immunosuppressionand increased mortality. Circulating cortisol levels may alsobe a factor in determining the outcome of social interactionsin pairs of rainbow trout, and hence in determining social status.Rainbow trout treated with cortisol were significantly morelikely to become subordinate in paired encounters with smalleruntreated conspecifics. 相似文献
4.
Summary Adrenergic innervation of the human gall bladder was studied using two specific fluorescence histochemical methods. Blue-green fluorescing varicose nerves were scarce and mostly followed the course of blood vessels as typical perivascular plexuses. However, some adrenergic nerves not associated with the vessels were occasionally seen, as well as structures suggestive of a pericellular arrangement of varicose adrenergic nerve terminals on non-fluorescing ganglion cells. A few enterochromaffin cells were seen in the epithelial lining, also in the deep invaginations obviously representing the Aschoff-Rokitansky sinuses. Occasionally, small rounded cells with a rounded, relatively large nucleus, and exhibiting a weak yellow-green to blue-green granular cytoplasmic fluorescence, were observed in the wall of the gall bladder. The possible functional and evolutionary significance of these neural and endocrine elements was discussed against the data on physiological and pharmacological studies obtained from the literature. It was concluded that their significance is, in all probability, secondary to the influence of the intestinal polypeptide hormones, vagal innervation and circulating catecholamines upon the normal function of the gall bladder. The glyoxylic acid-induced fluorescence histochemical method was found to be superior to the conventional formaldehyde technique in studies on human tissue. 相似文献
5.
DiBattista JD Levesque HM Moon TW Gilmour KM 《Physiological and biochemical zoology : PBZ》2006,79(4):675-687
To assess the effects of subordinate social status on digestive function, metabolism, and enzyme activity in salmonid fish, juvenile rainbow trout Oncorhynchus mykiss were paired with size-matched conspecifics (<1.5% difference in fork length) for 5 d. Fish that were fasted for 5 d and fish sampled directly from the holding tank were used as control groups. Both subordinate and fasted fish experienced significant decreases in intestine mass (P = 0.043), and the gall bladder showed marked and significant changes in both size (P = 0.004) and appearance. These findings suggest that the negative effect of social subordination on digestive function reflects in large part a lack of feeding. Hepatic phosphoenolpyruvate carboxykinase activity was significantly higher in subordinate fish relative to dominants, whereas subordinate hepatic pyruvate kinase activity was significantly lower; activities of both enzymes were significantly correlated with plasma cortisol concentrations and behavior scores. Dominant-subordinate differences in the activities of these enzymes were eliminated by administration of the glucocorticoid receptor antagonist RU486, underlining a role for circulating cortisol in eliciting the differences. Significant increases relative to control fish were also detected in red and white muscles from subordinate fish in the activities of protein catabolic enzymes (aspartate aminotransferase, alanine aminotransferase, glutamate dehydrogenase). These differences occurred in the absence of any change in plasma free amino acid or ammonia concentrations, supporting an enhanced turnover of amino acids in muscle in subordinate fish. The results support the hypothesis that changes in metabolism, beyond those elicited by low food consumption, may be responsible at least in part for the low growth rates typical of subordinate fish and that these changes may be related specifically to circulating cortisol levels in subordinate fish. 相似文献
6.
Adrenergic innervation of the human gall bladder was studied using two specific fluorescence histochemical methods. Blue-green fluorescing varicose nerves were scarce and mostly followed the course of blood vessels as typical perivascular plexuses. However, some adrenergic nerves not associated with the vessels were occasionally seen, as well as structures suggestive of a pericellular arrangement of varicose adrenergic nerve terminals on non-fluorescing ganglion cells. A few enterochromaffin cells were seen in the epithelial lining, also in the deep invaginations obviously representing the Aschoff-Rokitansky sinuses. Occasionally, small rounded cells with a rounded, relatively large nucleus, and exhibiting a weak yellow-green to blue-green granular cytoplasmic fluorescence, were observed in the wall of the gall bladder. The possible functional and evolutionary significance of these neural and endocrine elements was discussed against the data on physiological and pharmacological studies obtained from the literature. It was concluded that their significance is, in all probability, secondary to the influence of the intestinal polypeptide hormones, vagal innervation and circulating catecholamines upon the normal function of the gall bladder. The glyoxylic acid-induced fluorescence histochemical method was found to be superior to the conventional formaldehyde technique in studies on human tissue. 相似文献
7.
ABSTRACTWe investigated the histological structure of the graylag goose (Anser anser) gall bladder. Sections of the gall bladder were stained with hematoxylin and eosin (H & E), Alcian blue (pH 2.5) for acid mucopolysaccharides, Gomori’s method for reticular fibers, Masson’s trichrome, periodic acid-Schiff (PAS) and Verhoeff’s elastin stain. The goose gall bladder was composed of a tunica mucosa, tunica muscularis and tunica adventitia or tunica serosa. The tunica mucosa formed regularly distributed simple isometric folds plus larger, less numerous, branched folds. The luminal surface was lined by tall columnar epithelial cells that stained for both acid and neutral mucopolysaccharides. The epithelial cells formed a discontinuous striated border of interdigitating microvilli on the luminal surface. Neither a lamina muscularis nor goblet cells were observed in the tunica mucosa. Unusual findings included branched mucosal folds, discontinuous microvilli and absence of an outer longitudinal layer in the tunica muscularis. No marked sex-associated differences were found. The general histochemical and histological structures of the graylag goose gall bladder are similar to those of birds such as chukar partridge and quail, but with some unique elements that may reflect differences in organ function. 相似文献
8.
The factors influencing the migration of gall stones are ill understood. Altogether 331 patients undergoing cholecystectomy were studied prospectively. The diameters of the cystic and common bile ducts and of stones in the gall bladder and bile ducts were measured. Increasing pressure was applied to the freshly excised gall bladder in an attempt to evacuate stones through the cystic duct. Stones passed in 33 (60.0%) of patients with choledocholithiasis, 45 (67.2%) of patients with pancreatitis, and 7 (3.2%) of patients without either pancreatitis or choledocholithiasis. Stones migrated in 6 (3.0%) who had a normal cystic duct diameter (less than or equal to 4 mm) and in 46 (32.5%) with a duct over 4 mm diameter. Common bile duct stones were often larger than the diameter of the cystic duct and when reintroduced into the gall bladder would not migrate. The passage of debris (less than or equal to 1 mm) through the cystic duct bore no relation to the presence or absence of choledocholithiasis or a dilated cystic duct. Small stones (1-4 mm diameter) must migrate to initiate and facilitate further migration; some must increase in size in the common bile duct. Increased biliary pressure consequently dilates the duct system retrogradely, allowing larger stones to follow. Patients at risk of stone migration and thereby pancreatitis and jaundice have large ducts that can be detected by ultrasound assessment. 相似文献
9.
The gall bladder of eight dogs 3 months old were extracted and studied by the Holme's and Gross-Bielschowsky techniques. The nerves were found to form an extensive network within the wall of the gall bladder. However, 5 plexuses were identified and their arrangement was similar to that in the wall of the intestine. Nerve cells were only found in relation with the myenteric plexus. Moreover, knob-like terminals and circular type of nerve endings were noticed on the muscularis. The findings of the intrinsic innervation of the gall bladder of the dog were compared with that of man, monkey and guinea pig and the significance of that innervation was discussed. 相似文献
10.
A glutamine dependent carbamyl phosphate synthetase has been detected in the extra mitochondrial fraction of gall bladder mucosal cells obtained from rabbits and cattle. This enzyme is inhibited by azaserine. Thus, the enzyme appears to be carbamyl phosphate synthetase II. The activity of the preparation obtained from rabbits is four to seven times that of the bovine gall bladder. 相似文献
11.
Dr. A. F. Hayward 《Cell and tissue research》1965,65(3):331-339
Summary The electron microscopy of the gall bladder epithelium in the sheep shows that the cells are secretory. They have an extensive Golgi apparatus and sparse endoplasmic reticulum with secretory droplets localised in the apical region and have been referred to the group known as mucoid cells. The intercellular spaces which are elaborately developed in those species whose gall bladder is primarily absorptive are poorly developed. Pinocytosis is not a prominent feature. It is believed that the features noted are correlated with the reported absence of absorption in the ungulate gall bladder. 相似文献
12.
A volumetric method has been developed which permits continuous registration of volume flows across epithelial tissues. The method was applied to volume flow measurements across rabbit gall bladder epithelium. The rate of fluid reabsorption measured in this way was twice as high as previously observed in sac preparations of the gall bladder. This is probably due to better aeration and stirring of the mucosal solution. It was demonstrated that electrical gradients across the gall bladder induced volume flows towards the negative electrode. In non-transporting bladders volume flows were linearly related with current between 300 and 900 μA in both directions. However, volume flow rates were three times higher from mucosa to serosa than in the opposite direction. From the magnitude of polarization potentials, observed after switching off the current, the conclusion was reached that all of the current-induced volume flow is an osmotic flow due to salt polarization in the unstirred layers of the tissue. By implication, so-called streaming potentials observed during osmotic flows reflect solely polarization effects. In actively transporting gall bladders a 200 μA current increased or decreased the flow rate twice as much as expected from polarization effects alone. Therefore passage of current interfered directly with the active transport mechanism of gall bladder epithelium. 相似文献
13.
Male rhesus monkeys unfamiliar with each other were paired in a cage, and blood samples were collected before and a few hours after pair formation. Adrenocorticotrophic hormone (ACTH) and cortisol levels in each blood sample were measured. Dominant-subordinate status was ascertained through two rank tests, the food competition test and the agonistic behavior test, which were performed immediately after pair formation. As a result, the dominance relationship was determined in seven pairs formed from five animals, and the differences in ACTH and cortisol values between the dominant and subordinate animal in these pairs were compared statistically. The day after the first encounter, a second encounter was conducted in randomly selected pairs of monkeys. In the first encounters, higher levels of both ACTH and cortisol were detected in dominant animals in comparison to subordinate animals. Changing the animal's partner altered the stress responses whenever the animal's dominant-subordinate status changed. The elevated levels of ACTH and cortisol in dominant animals disappeared on the day after the first encounter. In dominant animals, the pituitary-adrenocortical stress response reacts sharply to situational demands, whereas subordinate animals have a weaker response. This acute stress response is different from a chronic stress response. When the subordinate animal cannot escape, its hypothalamic-pituitary-adrenocortical axis appears to be suppressed. 相似文献
14.
The gall bladder from 6 Psammophis sibilans, 10 Bufo regularis and 10 Albino mice were extracted and prepared for microscopic examination. It was found that the mucosa of Psammophis sibilans consisted of ovoid and polygonal cells which were occasionally binucleated cells with darkly stained nuclei and occasionally pear-shaped cells with vesicular nuclei and fine processes. These cells were arranged in three layers. Apossible explanation for the different types of cells encountered and their arrangement was given. The gall bladder mucosa of Bufo regularis and Albino mouse were thrown into folds covered with simple columnar epithelium. However, the epithelium of the frog was higher than that of the mouse, with the nuclei situated midway between basement membrane and the lumen. Vacuolated cells were detected in the gall bladder mucosa of the mouse. The significance of the mucosal folds was discussed. 相似文献
15.
Plasma cortisol concentrations before and after social stress in rainbow trout and brown trout 总被引:1,自引:0,他引:1
Sloman KA Metcalfe NB Taylor AC Gilmour KM 《Physiological and biochemical zoology : PBZ》2001,74(3):383-389
Two related experiments examined the relationship between plasma cortisol concentrations and the development of social hierarchies in fish. In the first, rainbow trout, Oncorhynchus mykiss, and brown trout, Salmo trutta, were observed for dominance interactions when confined within single-species pairs for 4, 48, or 168 h. Subordinate members of a pair exhibited significantly higher cortisol concentrations than dominant and single fish, but the pattern of cortisol elevation differed between the two species, being quicker to rise and increasing to a higher level in rainbow trout. Cortisol concentrations were correlated with behavioural measurements; the more subordinate the behaviour exhibited by a fish, the higher its cortisol concentration. Social stress was a chronic stressor, and no acclimation to social status occurred during the week. In the second experiment, measurements of plasma cortisol were made before pairing of rainbow trout and then after 48 h of confinement in pairs. Subordinate fish demonstrated significantly higher concentrations of plasma cortisol both before and after social stress. It therefore appears that in addition to cortisol being elevated during periods of social stress, an association may exist between initial cortisol levels and the likelihood of a fish becoming subordinate. 相似文献
16.
《Animal : an international journal of animal bioscience》2013,7(3):357-377
Behavioural adaptation of farm animals to environmental changes contributes to high levels of production under a wide range of farming conditions, from highly controlled indoor systems to harsh outdoor systems. The genetic variation in livestock behaviour is considerable. Animals and genotypes with a larger behavioural capacity for adaptation may cope more readily with varying farming conditions than those with a lower capacity for adaptation. This capacity should be exploited when the aim is to use a limited number of species extensively across the world. The genetics of behavioural traits is understood to some extent, but it is seldom accounted for in breeding programmes. This review summarizes the estimates of genetic parameters for behavioural traits in cattle, pigs, poultry and fish. On the basis of the major studies performed in the last two decades, we focus the review on traits of common interest in the four species. These concern the behavioural responses to both acute and chronic stressors in the physical environment (feed, temperature, etc.) and those in the social environment (other group members, progeny, humans). The genetic strategies used to improve the behavioural capacity for adaptation of animals differ between species. There is a greater emphasis on responses to acute environmental stress in fish and birds, and on responses to chronic social stress in mammals. 相似文献
17.
This study was carried out to investigate the occurrence of ciliatocholic acid in bovine gall bladder bile. Ciliatocholic acid was synthesized according to the method described by Bergstr?m and Norman for the synthesis of taurocholic acid. Elemental analysis, melting point, and the infrared spectrum of this substance were determined. An isolation procedure for ciliatocholic acid was established by stepwise elution with an HCl-ethanol solvent system using a Dowex-1 anion exchange resin column chromatographic technique. Ciliatocholic acid amounting to 158 mug (as ciliatine) per 100 ml of gall bladder bile was found in the fraction eluted with 0.01 N HCl in 50% ethanol. This coumpound was purified by preparative thin-layer chromatography and confirmed to be ciliatocholic acid from the hydrolytic stability, phosphorus determination, and chromatographic behavior. Thus, bovine gall bladder bile contains a small amount of ciliatocholic acid. 相似文献
18.
Yoshihisa Abe 《Oecologia》1992,89(2):166-167
Summary Gall clusters of Andricus symbioticus secrete a sweet and sticky food attractive to ants. An ant exclusion experiment demonstrated the selective advantage of attending ants and gall aggregation for A. symbioticus. This gall wasp interacts with the gall-attending ants only through the host plant. Evolution of this symbiotic relationship seems to be associated with gall aggregation.Contribution from Laboratory of Entomology Kyoto Prefectural University, No. 249 相似文献
19.
P. Knipschild 《BMJ (Clinical research ed.)》1988,297(6663):1578-1581
In alternative health care iridology is used as a diagnostic aid. The diagnosis of gall bladder disease was used to study its validity and interperformer consistency. The presence of an inflamed gall bladder containing gall stones is said to be easily recognised by certain signs in the lower lateral part of the iris of the right eye. Stereo colour slides were made of the right eye. Stereo colour slides were made of the right eye of 39 patients with this disease and 39 control subjects of the same sex and age. The slides were presented in a random order to five leading iridologists without supplementary information. The prevalence of the disease was estimated at 56%. The median validity was 51% with 54% sensitivity and 52% specificity. These results were close to chance validity (iota = 0.03). None of the iridologists reached a high validity. The median interperformer consistency was 60%. This was only slightly higher than chance consistency (kappa = 0.18). This study showed that iridology is not a useful diagnostic aid. 相似文献
20.
Rodlet cells within the epithelial mucosa of the gall bladder of the estuarine killifish Fundulus heteroclitus (L.) obtained from a highly contaminated creek adjacent to a landfill, were arranged within the constraints of the epithelium. Furthermore, the rodlet cells established a close intimate association with electron dense epithelioid cells. A comparison with fish from a non impacted estuary revealed a significantly greater number of rodlet cells in the 'contaminated' group. The abundance of rodlet cells within the gall bladder of the fish exposed to contaminants further strengthens the hypothesis that these cells participate in the fish's immune system. 相似文献