东北地区绿色发展水平时空动态演变与障碍因素

以"人地协调"发展理念和"过程+格局+机制"思维范式为切入点,构建了基于DPSIR模型的绿色发展水平指标体系,运用熵权TOPSIS模型、障碍度模型和GIS空间可视化等方法对2008-2017年东北地区各城市的绿色发展水平时空分异特征与障碍因素进行实证研究。结果表明：①研究期内东北地区各城市绿色发展水平总体呈现从"小差距低水平"逐渐向"大差距高水平"转变的时序特征,空间分布大致呈现自北向南阶梯式递减的格局特征,且空间集聚效应显著。②东北地区各城市经济发展水平与绿色发展水平之间并非完全呈正相关关系,即,经济发展水平越高的城市,绿色发展水平不一定越高;而经济发展水平较低的城市,绿色发展反而达到较高的水平。这可能与区域资源环境承载力的大小有关。③东北地区各城市绿色发展水平时空分异的作用机制不尽相同,其中,资源禀赋条件、经济发展状况、政府投资规模在绿色发展水平的变化中起着重要的作用,同时也是导致东北地区绿色发展水平不同阶段呈现不同进化特征的主要原因。     

Effect of between-category similarity on basic level superiority in pigeons

Children categorize stimuli at the basic level faster than at the superordinate level. We hypothesized that between-category similarity may affect this basic level superiority effect. Dissimilar categories may be easy to distinguish at the basic level but be difficult to group at the superordinate level, whereas similar categories may be easy to group at the superordinate level but be difficult to distinguish at the basic level. Consequently, similar basic level categories may produce a superordinate-before-basic learning trend, whereas dissimilar basic level categories may result in a basic-before-superordinate learning trend. We tested this hypothesis in pigeons by constructing superordinate level categories out of basic level categories with known similarity. In Experiment 1, we experimentally evaluated the between-category similarity of four basic level photographic categories using multiple fixed interval-extinction training (Astley and Wasserman, 1992). We used the resultant similarity matrices in Experiment 2 to construct two superordinate level categories from basic level categories with high between-category similarity (cars and persons; chairs and flowers). We then trained pigeons to concurrently classify those photographs into either the proper basic level category or the proper superordinate level category. Under these conditions, the pigeons learned the superordinate level discrimination faster than the basic level discrimination, confirming our hypothesis that basic level superiority is affected by between-category similarity.     

Prey dispersal and predator persistence

To understand how patchiness influences population dynamics of a tri-trophic interaction, a tractable model is formulated in terms of differential equations. Motivated by the structure of systems such as plants, phytophagous mites and predatory mites, the model takes dispersal into account at the middle trophic level. The effect of dispersal for the middle level in a tri-trophic system could be either stabilising or destabilising since the middle level acts both as prey and as predator. First a simple model with logistic growth for the lowest level is formulated. A model with logistic growth for the lowest level and instantaneous dispersal has a globally stable three-species equilibrium, if this equilibrium exists. Addition of a middle level dispersal phase of non-negligible duration influences equilibrium stability. In the absence of the top trophic level a limit cycle can occur, caused by the delay that exists in the reaction of the middle level to the changes in the lowest level. With low predator efficiency, it is also possible to have an unstable three-species equilibrium. So addition of a middle level dispersal phase of non-negligible duration can work destabilising. Next the persistence of the third trophic level is studied. Even when the three-species equilibrium exists, the third trophic level need not be persistent. A two-species limit cycle can keep its stability when a three-species equilibrium exists; the system is then bistable. It is argued that such a bistability can offer an alternative explanation for pesticide-induced outbreaks of spider mites and failure of predator introduction.     

Students' cellular and molecular explanations of genetic phenomena

Genetic concepts are assigned to three principal levels of organisation: macroscopic level, microscopic level, and submicroscopic level. In order to probe Israeli students' understanding at each level, and to gain a sense of their ability to connect ideas and concepts across different levels, we asked three different types of question. Two of the question types ask students to make a bridge between levels by asking them to explain a phenomenon at one level using concepts and processes from a different level. For example, to explain the appearance of phenotypic traits (macroscopic level) using concepts like genes or chromosomes (microscopic level). One question dealt with the molecular level only. We investigated three populations: 9^th graders, 12^th graders, and pre-service teachers. Based on our findings we suggest improvements both in terms of teaching methods and curriculum content.     

Cholinesterase Levels in Blood Plasma and Erythrocytes from Calves,Normal Delivering Cows and Cows Suffering from Parturient Paresis

Plasma cholinesterase (pChE) levels and erythrocyte acetylcholinesterase (eAChE) levels were studied in 6 cows before, during and after parturition (Group I), their calves (Group II), 38 cows suffering from parturient paresis (Group III) and 14 newly delivered non-paretic cows (Group IV). The mean of the pChE level in Group I was 1.5 μkat/1 ± 0.20 before parturition and decreased significantly (P ≦ 0.05) to 1.2 ukat/1 ± 0.16 after parturition. The eAChE level was before parturition ≅ 140 ukat/1 and decreased to ≅ 130 μkat/1 4–5 weeks after parturition. At birth the pChE level was 12.8 ukat/1 ± 5.9 in Group II. After 4 weeks the level had decreased to 2.3 ukat/1 ±0.3. In the bull calves the pChE level started to increase when they were 6 weeks old and reached a level of 5.7 μkat/1 ± 0.6 before slaughter at 6 months of age. The heifers did not show this increase. They had a level of around 2 μkat/1 throughout the investigation. The eAChE level at birth was 119 μkat/1 and increased slowly to a level of 145 μkat/1 at 6 months. No differences between the sexes were found. The cows suffering from parturient paresis had a pChE level of 1.80 μkat/1 ± 0.30 before treatment with calcium (Ca). The level decreased significantly (P ≦ 0.001) after Ca-infusion to a level of 1.67 ukat/1 ±0.29. Group IV had a pChE level of 1.65 μkat/1 ± 0.42 at parturition. Two to 4 months later the cows that had recovered from milk fever had a level of 1.61 μkat/1 ± 0.31 and the control cows 1.66 ukat/1 ± 0.48. No differences between the groups were found for the eAChE level. The findings show that parturition influences the pChE level in cows and that sex influences the pChE level in calves between 6 weeks to at least 6 months of age. Furthermore the elevated pChE level found in the cows suffering from parturient paresis before Ca infusion may be a further sign of a disturbance in the cholinergic system with a special preference to the neuromuscular junctions.     

Changes in the Serum Urate Level Can Predict the Development of Parkinsonism in the 6-Hydroxydopamine Animal Model

Epidemiological studies indicate that a higher plasma level of uric acid (UA) associates with the reduced risk of Parkinson’s disease (PD). To confirm the role of UA as a biomarker for PD, we evaluated changes in the serum UA level in the 6-hydroxydopamine (6-OHDA)-induced hemiparkinsonism in rat. For this purpose, 6-OHDA was administered in the medial forebrain bundle by stereotaxic surgery. According to the apomorphine-induced rotational test, the increased intensity of behavioral symptoms as a function of time was associated with the further reduction of UA level. On the other hand, the level of UA increased in the midbrain of the injured hemisphere. The level of reduction in the serum UA level of rats with severe and moderate symptoms was significantly higher than that of rats with mild symptoms. The immunohistofluorescence and biochemical analyses showed that the serum UA level was also correlated with the death of tyrosine hydroxylase (TH)-positive neurons in the substantia nigra pars compacta (SNc), reduced level of striatal dopamine, and severity of oxidative stress in the midbrain. The rats with mild symptoms also showed a significant decrease in TH-positive neurons and striatal dopamine level. These findings suggest a positive correlation between the level of reduction in the serum urate level and severity of 6-OHDA-induced Parkinsonism. In addition, our findings indicated that UA had no marked neuroprotective effects, at least at concentrations obtained in this study. On the other hand, UA was introduced as a biomarker for PD, as a significant decline was observed in the serum UA level of rats with mild behavioral symptoms but with significant dopaminergic cell death in the SNc.     

长三角地区城市生态旅游发展水平时空演变及其影响因素

城市生态旅游作为旅游可持续发展的一种理想模式,是对城市环境问题和旅游发展方式的一种回应。基于城市生态旅游核心要义,构建城市生态旅游发展水平评价指标体系,运用熵权TOPSIS法对2010-2019年长三角地区41个城市的城市生态旅游发展水平进行测度,通过标准差椭圆和马尔科夫链分析其时空演变特征,并利用地理探测器探析城市生态旅游发展的影响因素。结果表明:(1)长三角地区城市生态旅游各维度发展水平增速各异,供给和保障是影响综合发展水平的主要因素,各维度空间格局差异显著;(2)城市生态旅游综合发展水平呈波动上升态势,增速较缓;空间分布较不均衡,已初步形成"东南高-西北低"的分布格局;(3)城市生态旅游发展水平重心呈现"西北-东南-西北"的偏移规律,向东南部集聚程度逐渐增强,水平等级转移仅发生在相邻等级之间;(4)交通发展水平和城镇化水平对城市生态旅游发展的影响较为显著,经济发展水平和居民收入水平的影响逐渐增强,产业结构水平和环保重视程度的促进效果不显著;两因子的交互作用大于单因子的作用力,表现出双因子增强或非线性增强的交互关系。研究结果可为长三角地区提高城市生态旅游发展水平与全面推进旅游可持续发展提供一定的理论依据与科学参考。     

Synthesis and degradation of nitrite reductase in pea leaves

We have shown in a previous publication (Gupta, Beevers 1983 J Exp Bot 34: 1455-1462) that the level of extractable nitrite reductase activity in pea (Pisum sativum cv Burpeeana) leaves is subject to environmental perturbations. In the current study, we have used rocket immunoelectrophoresis to quantitate the level of nitrite reductase protein in extracts from pea plants subjected to various environmental treatments. The level of nitrite reductase cross-reacting material closely followed the level of assayable nitrite reductase activity. The environmental conditions which enhanced the level of extractable nitrite reductase activity resulted in an increased level of nitrite reductase cross-reacting material in the extracts. In contrast, environmental conditions which resulted in a decrease in the level of extractable nitrite reductase activity produced a decline in cross-reacting material. These results indicate that the environmentally induced modulation of extractable nitrite reductase activity involves alteration of enzyme level and is not mediated by a reversible activation-inactivation of the existing enzyme.     

Comparison of Transmitter Amino Acid Levels in Rat Globus Pallidus and Neostriatum During Hypoglycemia or After Treatment with Methionine Sulfoximine or γ-Vinyl γ-Aminobutyric Acid

The levels of amino acids in globus pallidus, a structure heavily innervated with gamma-aminobutyric acid (GABA)-ergic terminals but few glutamergic terminals, were compared with the levels in neostriatum, a structure richly innervated with glutamergic terminals but intermediate in GABAergic terminals. The level of glutamate in neostriatum was twice as high as in globus pallidus whereas the level of GABA in globus pallidus was three times higher than in neostriatum. The level of aspartate was similar in both regions whereas the level of glutamine was correlated with the level of glutamate. Methionine sulfoximine, a glutamine synthetase inhibitor, reduced the level of glutamine to 10-20% of control in both structures. This reduction was accompanied by the largest decrease in the level of glutamate in neostriatum, indicating that transmitter glutamate turns over more rapidly than other glutamate pools. Likewise, insulin decreased the levels of glutamate and glutamine more in neostriatum than in globus pallidus. gamma-Vinyl GABA increased the level of GABA in globus pallidus more than in neostriatum although the percent increase was largest in neostriatum. Treatment with gamma-vinyl GABA was accompanied by a large reduction in the level of GABA, indicating that a substantial proportion of the glutamine pool is linked to GABA metabolism.     

Dynamics of various integral characteristics of individual changes in ethanol resistance in isolated muscles of grass frog]

A study was made of the dynamics of the mean level of toxic ethyl alcohol (EA) (3.5 M) resistance, of the correlation between the initial individual level of this resistance and the value and shifting direction of the level, and of the pattern of correlation between levels of resistance in the pair sartoria muscles and variability of resistance level in muscles, kept in EA subtoxic solutions (0.87 and 1.09 M) for 240 min. Two-phase changes in the mean level of resistance in muscles were revealed that involved its increase by 20% within 60 min of maintenance in both EA concentrations, and followed by a decrease of this level by the end of observation. On the first steps of muscle maintenance in EA subtoxic solutions, a negative correlation was noticed between the initial level of resistance and the value and direction of its shifting, in addition to narrowing the range of variability of this level in muscles. All these events were accompanied by a decrease or fluctuations in the correlation coefficient between levels of resistance in the pair muscles. By the end of the experiment, the range of variability of the level of resistance was seen to increase, and the earlier negative correlation between the initial individual level of resistance and the value and direction of its shifting appeared to change for a positive one. Both processes preceded the decrease in the mean level of muscle resistance.     

Intrapopulation genetic variation in the level and rhythm of daily activity in Drosophila immigrans

Genetic diversity within a population, such as polymorphisms and personality, is considered to improve population performance because such intraspecific variations have the potential to alleviate the competition for a limited resource or the risk of predation and sexual harassment at a population level. Variation in the level and rhythm of daily activity in a population could also affect population performance by directly altering ecological, social, and sexual interactions among individuals. However, it remains to be elucidated whether such intra‐population variation in the level and rhythms of daily activity exists in a natural population. Here, we investigated the genetic variation in daily activity within a single natural population of Drosophila immigrans. We established 21 isofemale lines from a single natural population and measured larval activity level and the level and daily pattern of adult activity over a 24 hr period. Larval activity level significantly varied among isofemale lines. Likewise, the activity level in the adult stage significantly varied among lines. The significant variation was also found in the daily pattern of adult activity; some lines showed greater activity level in the daytime, and others showed greater activity level in the night. Our results consistently suggest that there is a genetic variation in behavioral activity in a natural population, probably contributing to shaping the population performance.     

A Markov decision model for computer-aided instruction

A mathematical model for computer-aided instruction is developed. It is assumed that the course is divided into a hierarchy of levels of difficulty. These levels are such that if a student is able to perform successfully at a given level of difficulty, he can also perform successfully at all levels of lesser difficulty. Furthermore, if student performs successfully at one level, it increases his probability of being able to perform successfully at the next higher level of difficulty. Given the initial vector of probabilities for successful performance at each level, the vector describing how these probabilities change with successful performances at each level, and the expected times it takes to attempt a successful performance at each level, this model computes an instructional sequence that minimizes the expected time required for the student to complete the course by performing successfully at the highest level of difficulty. Dynamic programming is used to find this sequence.     

Heat shock-induced, caspase-3-independent rapid breakdown of Akt and consequent alteration of its total phosphorylation/activity level

The immediate effect of a 15-min heat shock was examined on the level and the activity of Akt. Following heat shock, the Akt level decreased by 15-70% in a temperature-dependent and phosphorylation status-independent manner. This decrease of Akt level was not prevented by caspase inhibitors. At 48 degrees C, the extent of the breakdown was so immense that the total phosphorylation/activity level of Akt was not increased over the control level, implying that the total cellular activity of Akt governed by the level and the molar activity does not necessarily undergo the ensuing change.     

Modified cyclic nucleotide systems in Morris hepatoma 3924A favoring expression of cyclic GMP effect.

Modifications in the cyclic nucleotide systems favoring the expression of cyclic GMP effects were found to occur in the transplanted fast-growing Morris hepatoma 3924A. These included: (a) a decreased level of cyclic GMP phosphodiesterase and an increased level of cyclic AMP phosphodiesterase; (b) a disproportionately increased level of cylic GMP-dependent protein kinase relative to that of cyclic AMP-dependent protein kinase; (c) a disproportionately increased level of stimulatory modulator of cyclic AMP-dependent protein kinase relative to that of inhibitory modulator of cyclic AMP-dependent protein kinase; and (d) an increased level of phosphoprotein phosphatase.     

基于水质管理目标的博斯腾湖生态水位研究

人类开发活动导致湖泊生态功能严重退化,研究湖泊生态水位对于维持湖泊生态系统健康意义重大。针对博斯腾湖化学需氧量(COD)浓度较高的水环境现状,分析博斯腾湖水位和COD浓度关系,研究提出基于水质管理目标的生态水位,以期为博斯腾湖水资源、水环境管理提供参考。结果表明,博斯腾湖水位与水体COD浓度显著负相关,但由于COD浓度空间差异较大以及影响因素不唯一,水位与COD浓度两者之间曲线估计结果不理想。为实现博斯腾湖COD浓度小于20 mg/L的水质管理目标,引入累计水位概念进行统计分析得到两个特征水位:所有COD浓度大于等于20 mg/L的数据对应水位的平均值为1046.02 m,该水位在历史丰水期水位的频率为60.83%,可作为最小生态水位;所有COD`浓度小于等于20 mg/L的数据对应水位的平均值为1046.4 m,该水位在历史丰水期水位的频率为44.70%,可作为适宜生态水位。适宜生态水位1046.4 m与已有研究成果基本相符,博斯腾湖在1046.4 m时既有利于水质管理,也可保障湖泊整体生态系统健康。     

EEG frequency: amplitude characteristics at different levels of consciousness

Relationship was studied between the level of consciousness and the level of the brain activation. State of transition from wakefulness to drowsiness was used as a model of gradual decrease in the level of consciousness. A change in the intensity and quality of appearing involuntarily mental processes served as an index of the level of consciousness as determined by subjects' self-reports. It was found that a certain EEG profile corresponded to each level of consciousness under study. The data obtained showed that the lowest level of activation corresponded to a state in which a subject develops inhibition of "internal speech", "failure of thoughts". In this case, the dynamics of the EEG high frequency rhythm changes was possibly related to the mechanism of transmission of cognitive information.     

Modified cyclic nucleotide systems in Morris hepatoma 3924A favoring expression of cyclic GMP effect

Modifications in the cyclic nucleotide systems favoring the expression of cyclic GMP effects were found to occur in the transplanted fast-growing Morris hepatoma 3924A. These included: (a) a decreased level of cyclic GMP phosphodiesterase and an increased level of cyclic AMP phosphodiesterase; (b) a disproportionately increased level of cyclic GMP-dependent protein kinase relative to that of cyclic AMP-dependent protein kinase; (c) a disproportionately increased level of stimulatory modulator of cyclic GMP-dependent protein kinase relative to that of inhibitory modulator of cyclic AMP-dependent protein kinase; and (d) an increased level of phosphoprotein phosphatase.     

Genetic control of basal level of alkaline phosphatase in Escherichia coli

Summary The basis for a difference in basal levels of alkaline phosphatase in two strains of E. coli has been examined. It was found that the enzyme level characteristic of a highlevel strain was not conferred upon a low level strain merely by the transfer of the pho ⁺, phoR⁺ or phoS ⁺ allele from the high-level strain to the low level strain. Such strains may have slightly altered basal levels of enzyme, but these changes are not quantitatively sufficient to account for the strain differences. Approximately 20% of the recombinants which receive the phoS ⁺ gene show a level of phosphatase intermediate between the high and low-level strains. The effect is specific for the basal level of phosphatase, since it does not affect the levels of three other enzymes, or the derepressed level of alkaline phosphatase itself. This latter fact is consistent with the view that the intermediate level strains have an intermediate level of repressor of alkaline phosphatase under repressed conditions. It is suggested that the basal level of enzyme is affected by several genes in addition to those previously described, and that the intermediate level strains have received the allele of one or more of these loci from the high level strain.     

The impact of cerebral palsy on the intelligibility of pitch-based linguistic contrasts

This study investigated the perception of three Cantonese level tones produced by speakers with dysarthria associated with cerebral palsy. Four speakers with dysarthria were selected on the basis of their distinctive patterns of fundamental frequency (F0) values observed in the level tones they produced, which showed errors in either F0 level or, F0 contour, or both. Monosyllabic words which contrasted in tone level were used as stimuli in an identification task. Five expert listeners identified the tones among the six Cantonese contrastive tones. Results showed that the tones produced by the dysarthric speakers were predominantly perceived as level tones; although a majority was perceived as the wrong level tone. The most important finding is that only the level tones produced by dysarthric speakers could be identified as rising or falling contour tones. The frequent perceptual confusion among the level tones, and the perception of contour tones, shows that a disorder in the production of pitch-based linguistic contrasts can have a substantial impact on the communication abilities of individuals with cerebral palsy.     

Effect of norepinephrine and dibutyryl cyclic AMP on S-100 protein level in C6 glioma cells

S-100 Protein level was determined in C6 glioma cells after treatments by norepinephrine. In growing cells norepinephrine induces an important increase of S-100 protein level continuing during the stationary phase to reach a level higher than in untreated quiescent cells. In quiescent, low density, thymidine blocked cells, S-100 protein level is also enhanced by norepinephrine. In high density, contact inhibited cells, S-100 protein level is not modified although cAMP level is much more stimulated by norepinephrine than is low density cells. Exogenous addition of dibutyryl cyclic AMP mimics the effects of norepinephrine.Our results suggest that cyclic AMP level can mudulate S-100 protein level in C6 cells but that in density inhibited cells, a subsequent step involved in the regulation is no more operative.