The emergy ecological footprint for small fish farm in China

Mariculture, especially cage aquaculture remains a growing, vibrant and important production sector for high protein food in coastal regions in China. A quantitative evaluation of the mariculture system is an essential step to documenting its’ sustainability. The method of emergy ecological footprint is applied to evaluate the environmental sustainability of an offshore small fish farm (Great Marine fish farm, GMFF) in the East China Sea. All input needed to support fish farming were accounted and converted into biological space, to estimate the natural capital demand for the rearing process in terms of global hectares. The emergy ecological footprint of GMFF was 1953.19 ha, meaning that nearly 2000 ha of ecologically productive lands were needed to support the fish framing. The largest component of emergy ecological footprint was forage (1183.64 ha), which took up 60.60% of the total footprint; the second and third largest footprint components were fingerlings and fuel. In a word, emergy ecological footprint can serve as a practical and meaningful tool for comparing and monitoring the environmental impact of fish farming. The strong dependence of external contributions of exploiting the wild fish resources affects strongly the level of environmental sustainability of fish farming.     

The emergy of metabolism in different ecosystems under the same environmental conditions in the agro-pastoral ecotone of northern China

The sustainability of ecosystem productivity and rules governing ecosystem development are important topics of scientific research. The emergy approach is an effective method for investigating these topics, especially when used to evaluate systems that have developed under the same environmental conditions, such as climate and soil. In this paper, emergy differences between terrestrial ecosystems were studied in Guyuan County, a region representative of the agro-pastoral ecotone in Hebei Province, China. A combination of field tests and a questionnaire survey were carried out between June and August 2015. The ecosystems studied included natural grassland, artificial grassland, field crops and commercial crops. These four ecosystems were further subdivided into a total of ten ecosystems. Natural grassland was divided into free-grazing and mowed ecosystems; artificial grassland consisted of oat, Chinese leymus and corn silage; field crops included naked oats, flax and wheat; and commercial crops consisted of cabbage and potatoes. The results showed that the rain input of 4.78 × 10¹⁴ seJ/ha/yr constituted the highest renewable natural resource emergy and that the purchased emergy inputs of the ten ecosystems ranged from 3.53 to 147.67 × 10¹⁴ seJ/ha/yr. Natural resource emergy input was the basic power to maintain the ecosystem, and purchased emergy input was the direct cause of the development of the ecosystems. Groundwater was the most important non-renewable purchased energy for the production of economic crops. The emergy investment ratios (EIR) for potatoes (27.81) and cabbage (19.03) were higher than those of the other ecosystems, but mowed and artificial Chinese leymus grassland had the higher emergy self-sufficiency rates (ESR). Natural grassland, artificial Chinese leymus grassland and traditional grain crops had a low environmental load and high sustainability, whereas potatoes and cabbage had a high environmental load and low sustainability. Overall, rain-fed artificial grassland has a high development potential from the perspective of environment and productivity.     

Earthworms as bioindicator of metals (Zn,Fe, Mn,Cu, Pb and Cd) in soils: Is metal bioaccumulation affected by their ecological category?

The importance of earthworms in metal pollution monitoring is widely recognized in terrestrial ecosystems. Metal bioaccumulation by soil-dwelling earthworms can be used as an ecological indicator of metal availability in soils. In this study, we quantify the level of DTPA extractable metals in casts and tissues of earthworms (endogeic: Metaphire posthuma (Vaillant) and anecic: Lampito mauritii Kinberg) and ingesting soils, collected form cultivated land, urban garden and sewage soils. Soil and worm casts collected from sewage and cultivated land showed the greater metal concentrations. The concentration of Zn, Fe, Pb and Mn in earthworm casts was in the order: sewage soil > cultivated land > urban garden, while for Cu and Cd the order was cultivated land > sewage soil > urban garden. Data suggested that the level of DTPA extractable metals was higher than that of surrounding soils. We got close relationships between metal concentration in worm tissues and surrounding soils: Zn (r² = 0.94 and 0.89, P < 0.01 for both), Fe (r² = 0.95 and 0.97, P < 0.01 for both), Cu (r² = 0.93 and 0.96, P < 0.01), Pb (0.63, P < 0.01 and 0.57, P > 0.05), and Cd (r² = 0.15, P > 0.01 and 0.75, P < 0.01), respectively, for M. posthuma and L. mauritii. The study clearly indicates that earthworms have efficient potentials for bioaccumulation of metals in their tissues which can be used as an ecological indicator of soil contaminations. A species-specific metal accumulation pattern was observed in studied earthworms. Comparatively, endogeic showed the higher metal contents in their tissues than anecic (t-test: P < 0.05); collected form different habitats studied. Data suggested that species-specific feeding behaviour, earthworm niche structure, ecological category of inhabiting earthworm and even horizontal distribution of contaminants in soil layers are some major determinant for metal accumulation patterns in soil dwelling earthworms. The difference in burrowing patterns can influence the patterns of metal bioaccumulations between endogeic and anecic, although other factors are also contributory. Further more detailed study is still required to elaborate the proposed hypothesis.     

Emergy analysis of cassava chips-suitable feedstock for fuel ethanol in China

The theory and indices of Odum's concept of emergy are explained. The environmental and economic inputs and sustainability of cassava chips production system are evaluated by emergy methodology. The emergy indices of cassava chips production system were calculated as follows: Tr (transformity) was 6.85E + 11 sej/kg, EYR (emergy yield ratio) was 1.11, ELR (environmental loading ratio) was 1.75, EIR (emergy investment ratio) was 9.33, and ESI (emergy sustainability indice) was 0.63. The emergy indices of four kinds of feedstock for fuel ethanol—corn, wheat, sugarcane, and cassava chips—were compared. Least solar energy was consumed when taking cassava chips as feedstock for fuel ethanol. According to the emergy indices, using cassava chips as the feedstock of fuel ethanol is helpful for sustainable development in China.     

Emergy synthesis of a combined food and energy production system compared to a conventional wheat (Triticum aestivum) production system

It is a major challenge to produce food and energy sustainably for the ever increasing world population as today's conventional food production and energy needs are met by the fossil based resources, causing enormous environmental load. A novel, combined food and energy (CFE) agro-ecosystem, was designed for sustainable production of food, fodder and energy without chemical inputs (fertiliser, herbicide and fungicide). The objective was an emergy synthesis of the CFE system compared to a conventional wheat (Triticum aestivum) production system to assess resource use efficiency. The emergy indices, used to assess the environmental performance and sustainability, exhibited contrasting differences between the two production systems in terms of outputs (Y), total emergy use, solar transformity, relative use of local renewable resources, environmental loading ratio (ELR), emergy yield ratio (EYR) and emergy sustainability index (ESI). The Y in the CFE consisted of grain, straw, fodder and woodchip production of 4020, 3580, 6100 and 10,000 kg/ha/yr respectively whereas Y in the conventional wheat consisted of 7250 and 3770 kg grain and straw/ha/yr respectively. The Y in the CFE was 81% (2.80E+11 J/ha/yr) higher with 13.5 times (6.40E+03 seJ/J) lower solar transformity compared to the Y (1.54E+11 J/ha/yr) and solar transformity (8.63E+04 seJ/J) in the conventional wheat, exhibiting highly resource intensive production in conventional wheat. The local renewables constituted 19.2% and 2.6% of the total emergy input in the CFE and the conventional wheat respectively with a corresponding lower ELR (4.21) and 22.5% higher EYR (1.26) in CFE compared to conventional wheat. CFE was more reliant on local renewable emergy flows and compatible with the local environment with higher ESI (0.30) compared to conventional wheat (0.03), where 64.5% of the total emergy input constituted chemical inputs. The study demonstrated that the innovative agro-ecosystem, exemplified by CFE, is considerably less resource demanding and more amenable to sustainable production, whether defined in terms of outputs, solar transformity, relative use of local renewable resources, EYR, ELR or ESI.     

Emergy and exergy evaluation of a dike-pond project in the drawdown zone (DDZ) of the Three Gorges Reservoir (TGR)

The dike-pond system was a form of ecological engineering that was a component of successional dikes and ponds along the banks of the Pengxi River in the drawdown zone (DDZ) of the Three Gorges Reservoir (TGR). The application of science-based evaluation system was appropriate for the flows of emergy in this agricultural ecosystem. The Emergy Analysis (EmA) has the ability to transform different types of inputs to a common form to allow meaningful comparisons across different systems. This study made use of the emergy analysis that assessed two different types of farming methods in the DDZ of the TGR. One method was planning crops in a dike-pond system (model I), and the other method was conventional agriculture (model II). In addition, the Exergies of both yields of agriculture methods were calculated, and the Exergy and Emergy Density (ED) were combined to explore the quality of these methods. The results showed that the two models relied on different resources. The ED yield of both models were similar, but the emergy investment of model II was greater than that of model I. Model II also used less renewable energy input to the agricultural systems than model I. The agricultural emergy sustainability index (AESI) of model I system (AESI = 2.4 > 1) was greater than that of model II system (AESI = 0.5 < 1), which indicated that the sustainable development of model I was stronger than that of model II in the DDZ of the TGR. The ratio Exergy/Emergy density (R_ex/em) in the two models of different agricultural system were 121.52 × 10⁻³ J/sej (Model I) and 24.19 × 10⁻³ J/sej (model II). Model I was greater than model II, and the result intimated that the model I agricultural system was a new method in the DDZ, but it was older and closer to the steady state than model II. The Emergy and Exergy analysis certifies that model I has a more acceptable and more sustainable development potential and is more stabilized in the DDZ of the TGR.     

The prediction of ecological potential for developing salt-tolerant oil plants on coastal saline land in Sheyang Saltern,China

The current energy crisis is a worldwide problem, and developing energy from biomass is considered an effective partial solution. Sheyang Saltern is located in a coastal area in East China. The administration of saltern cooperated with our Halophyte Research Lab to create a Five-year Plan of Biomass Energy on Saline Land. In this plan, 3330 ha of salt-tolerant oil plants will be planted on the coastal saline land in Sheyang Saltern, including 2664 ha of Ricinus communis and 666 ha of Kosteletzkya virginica (L.) presl. These plants will produce biomass energy from land that is not fit for common crops, and thus this plan will not affect crop production. Moreover, the biomass of plants will be fully utilized in a hierarchical way, guided by an ecological engineering design. Thus, more outputs and more profits will be gained by applying this plan. This study uses the emergy analysis to predict and analyze the saltern ecosystem's ecological potential and compare it with an existing system founded in 2007, a 33.3 ha field of the two plants in the saltern. Four main emergy indices of the two ecosystems, EYR, EIR, ELR and ESI, are calculated and compared. To better show the system's ecological potential, we establish a new emergy index-potential yield ratio (PYR). PYR is designed to measure artificial ecosystems’ potential output capacity. It reflects a system's potential to transform stored emergy into output emergy using ecological engineering technology. The value of the predicted system's PYR is as great as 225, indicating that the system has a bright future to develop salt-tolerant oil plants. The system's future ecological potential and additional ways to utilize the two plants – the use of ricin and tuberous roots, etc. are also discussed in this paper. Our results will be helpful to the government's policy making, and provides useful information for enterprises.     

Application of the componential method for ecological footprint calculation of a Chinese university campus

The componential method of ecological footprint is applied to estimate the ecological footprint of Northeastern University (NEU) in 2003. The components considered in this study include energy (coal, natural gas, and electricity) consumption, food consumption, waste disposal, water supply, transportation, and paper consumption. The ecological footprint of NEU was 24,787 ha, meaning that nearly 25,000 ha of ecologically productive lands were needed to support consumptions and wastes disposal. The largest component of ecological footprint was energy consumption, which took up 68% of the total footprint; the second and the third largest footprint components were food consumption and waste disposal. In a word, ecological footprint can serve as a practical and meaningful tool for comparing and monitoring the environmental impact of campuses.     

Valuation of the loss of plant-related ecosystem services caused by water stress in the wetland of China’s Yellow River Delta

China’s Yellow River Delta is ecologically important because of its role as an eco-tone between terrestrial and aquatic ecosystems. However, water stress caused by drought or flooding creates ecological risks for this important ecosystem. In this study, we assessed community biodiversity, plant biomass, and the plant total nitrogen, total phosphorus, and potassium contents to quantify the potential loss of ecosystem services value arising from water stress. The annual ecosystem services and function value of the wetlands totaled 3.68 × 10⁸ Yuan, of which biomass production and local climate regulation accounted for 39.4% and 49.5% of the total, respectively. The area with the highest value (>2 Yuan m⁻²) lies along both banks of the downstream reaches of the river, whereas areas with the lowest values (<1.5 Yuan m⁻²) were located on the northern bank, near the Bohai Sea coastline. We defined scenarios based on three levels of water stress: drought, sufficient water, and flooding. The potential annual value loss in the drought scenario was 3.60 × 10⁸ Yuan, versus 2.78 × 10⁸ Yuan in the flooding scenario. The minimum loss (with sufficient water) was 2.06 × 10⁸ Yuan. The wetland’s soil water content should therefore be managed to protect the vegetation and minimize the ecological risks (and associated ecosystem service value losses) caused by water stress. Our approach provides a tool for assessing the potential loss of ecosystem services and functions and for calculating ecological compensation payments for wetland damage.     

Ecological energy accounting for the gambling sector: A case study in Macao

Macao is a population-dense society that depends heavily on exogenous natural resources. In parallel with an expanding gambling sector, tourism became the most important driving force in Macao's economy. Economic growth in Macao has now benefited from the gambling boom and associated tourism for more than two decades. Although traditional economic theory has been used to deal with the economics of the gambling sector, the materials and energy foundation for this sector has not been previously analyzed. This is an important omission from the literature, since the gambling sector provides gamblers with food, tickets, services, water, electricity, equipment, labor, and other services that consume large quantities of materials and energy. In this paper, emergy synthesis is introduced to analyze Macao's gambling sector in 2004. Macao's casinos provide a dense flow of services that favor gambling tourists, since the emergy/$ ratio (1.42 × 10¹² sej/$) for the sector is much lower than that for Macao as a whole (2.38 × 10¹² sej/$). The emergy imports by this sector totaled 78.1 × 10²⁰ sej, and its exports were 77.1 × 10²⁰ sej. The emergy of services therefore plays an important role in Macao's gambling sector, and the transformity of an employee in this sector (11.2 × 10¹⁶ sej/person) was much higher than that of a typical person in Macao (5.27 × 10¹⁶ sej/person).     

Feasibility of using constructed treatment wetlands for municipal wastewater treatment in the Bogotá Savannah,Colombia

The main water bodies in the Bogotá Savannah have been seriously polluted due to the mismanagement of domestic, agricultural, and industrial wastewater. While there are a number of wastewater treatment facilities in the region, most do not function properly. There is a great need for inexpensive and sustainable wastewater treatment systems that are not technologically sophisticated and that do not require intensive management. The main goal of this study was to quantify the performance and sustainability of treatment wetlands and existing wastewater treatment systems in this region. Using data from the literature, a treatment wetland model was developed, which focused on pollutant removal. The modeled performance was compared to a system of waste stabilization ponds and a sequencing batch reactor. The three systems were subject to cost analysis and an emergy evaluation, leading to the assessment of indicators of cost-benefit for comparison. The economic analysis suggested that the net annual cost of the treatment wetland was US$ 14,672, compared to US$ 14,201 for the stabilization ponds and US$ 54,887 for the batch reactor. The emergy evaluations show that the ponds have the lowest annual emergy flow (6.65 + 16 sej/yr), followed by the constructed wetland (2.88E+17 sej/yr) and the batch reactor (8.86E+17 sej/yr). These results were combined to estimate treatment ratios (contaminants removed per lifetime cost, and contaminants removed per total emergy), cost ratios (cost per volume of water, annual cost per capita, and construction cost per capita), and emergy ratios (treatment yield, renewable emergy, lifetime emprice, construction emprice, non-renewable emergy, empower density, environmental loading, total emergy per volume of water, and emergy per capita).     

Population health risk due to dietary intake of toxic heavy metals from Spinacia oleracea harvested from soils collected in and around Tshwane,South Africa

In order to assess the possible health risk associated with the consumption of vegetables harvested from waste dump sites, trace metal levels in Spinacia oleracea planted in soils collected from waste dump sites were investigated. Soil samples from different waste dump sites and mining areas were collected and placed in different pots. Seedlings of S. oleracea were introduced into the pots, harvested after 3 months and analysed for trace metal contents using ICP-MS. From the leaves of the plants, the concentration of Fe was found to be significantly higher than all other trace metals (p < 0.05). The trend in trace metal accumulation from the leaves was in the order Fe > Mn > Zn > Pb > Cu > Cr > Ni > Cd. A significantly different concentration of trace metals in the plant was noticed from different soils in different pots used (p < 0.05). Trace metal concentration from plant parts showed roots > leaves > stem. The risk to human health indicated as Hazard Quotient (HQ) was highest for Zn followed by Cu from all the plant parts. The HQ result showed that humans might be at risk if they consume spinach from these waste dump sites. From the study it was concluded that harvesting/consuming spinach from soil around a waste dump site may be extremely dangerous.     

Development and application of an ecological classification tool for fish in lakes in Ireland

A classification tool suitable for establishing the ecological status of lakes based on fish population parameters has been developed for the Republic of Ireland and Northern Ireland (EU Water Framework Directive Ecoregion 17). A lake typology relevant to fish populations in lakes from Ecoregion 17 was produced as part of the ecological classification tool development. Four lake types were determined based on fish metrics and abiotic variables from 43 “reference” lakes. The specific lake fish typology categorised lakes into low (≤67 CaCO₃ mg L⁻¹) or high (>67 CaCO₃ mg L⁻¹) alkalinity, and shallow (≤17 m) or deep (>17 m) maximum depth. The fish in lakes classification tool (FIL2) follows a novel multimetric predictive approach, assigning ecological status to a lake using two independent methods. FIL2 qualitatively defines a lake's ecological status based on fish metrics using discriminant classification rules and, using a generalised linear model, quantitatively derives an Ecological Quality Ratio (EQR, 0 < EQR < 1), along with associated confidence intervals. It is recommended that both methods are used to validate output and cross-check and highlight potential misclassification.     

Distribution and enrichment of heavy metals among sediments,water body and plants in Hengshuihu Wetland of Northern China

In this study, a survey for the spatial distribution of heavy metals in Hengshuihu Wetland of China was conducted. Samples were collected from three compartments, water, sediment, and reed (Phragmites communis Trin), at different sites, and their contents of heavy metals, including mercury (Hg), arsenic (As), chromium (Cr), lead (Pb), copper (Cu), zinc (Zn), and cadmium (Cd), were analyzed. The results showed heavy metals in the sediments distributed in the Buffer Zone and Wangkou Sluice area at concentrations relatively higher than those in other areas, while concentrations in the Core Zone were lower. The heavy metal concentrations of water bodies in all areas, except those for Hg and Pb, were lower than the cutoff values for the first-grade water quality that was set as the highest standard to protect the national nature reserves. The heavy metal distributions among the three compartments were significantly different, with the following order: sediment > plant > water. In the reeds, accumulated amounts of different heavy metals varied in the following order: Hg > Zn > As > Cu > Cr. Concentrations of heavy metals only showed weak correlations between the water bodies and the sediments. Concentrations of heavy metals (except Hg and Cr) had no corrections between the sediments and the reeds. The distribution of mercury indicated that it enters the lake mainly from the atmosphere and outside water bodies. The concentrations of As, Hg, Cr, Cu and Zn in different parts of the reeds were detected and their abundances were ranked in the following order: root > leaf > stem.     

Cadmium distribution and its effects on molybdate-containing hydroxylases in Phragmites australis

The influence of cadmium (Cd) on physiological and biochemical parameters was studied to elucidate the mechanism of Cd resistance in Phragmites australis. Cadmium concentrations in roots, stems and leaves increased with exogenous Cd concentration, but Cd content in roots was much higher than in shoots. X-ray microanalysis was used to reveal compartments in which Cd accumulated in root cortex. Cadmium concentrations followed a gradient with the sequence: intercellular space > cell wall > vacuole > cytoplasm, indicating that most Cd was immobilized in the apoplast or sequestered into the vacuolar lumen. Sequential extraction of various Cd chelates revealed that more than half of extractable Cd was bound to proteins, whereas 26% was bound to organic acids. Cd-binding protein fractions were found in the roots after gel filtration chromatography, among which a polypeptide with an apparent molecular mass of 14 kDa bound Cd most avidly. One newly synthesized polypeptide of low molecular mass (1 kDa) appeared under Cd pollution, whereas a prominent fraction of 72 kDa disappeared. Four aldehyde oxidase (AO) isoenzyme activities increased significantly in roots under Cd pollution. Cd stress also enhanced xanthine dehydrogenase (XDH) activities in roots. Two AO polypeptides of different molecular sizes were detected in the roots by Western blot assay. The abundance of the 160 kDa subunit correlated with Cd stress, but the amount of the 90 kDa polypeptide did not change under Cd treatment. Enhanced abscisic acid (ABA) contents were observed in roots of P. australis exposed to Cd. The involvement of Cd distribution in plant tissues and subcellular compartments and of AO and XDH enzymatic activities in the acclimation mechanism of P. australis to Cd pollution is discussed herein.     

Suitable sediment fraction for paleoenvironmental reconstruction and assessment of contaminated coastal areas based on benthic foraminifera: A case study from Augusta Harbour (Eastern Sicily,Italy)

Since the 1990s several studies noticed that, along coastal marine areas, the mean size of benthic foraminifera may be reduced due to heavy metal pollution, even if no biometric studies were carried out to quantify this aspect. The Augusta harbour (Sicily, Italy), is characterized by a strong contamination due to several anthropogenic activities, the most important of which are a petrochemical pole and an important industrial harbour. Taking into account the previous studies carried out in the area, which recorded small-sized foraminifera, the present study compared assemblage composition and faunal parameters in the >125 μm and >63 μm fractions of a sediment core collected in the most polluted sector of Augusta harbour. The aim was to understand if the two fractions have comparable environmental significance providing reliable information on the environmental status. In order to quantify the amount of smaller foraminifera in a community and to determine species loss between size fractions, two new indices are used: the Foraminiferal Size Index (FSI) and the Lost Species Index (LSI). Species richness, diversity and composition of the two assemblages were determined to characterize their structure. The results highlighted great depletion and different composition of the >125 μm assemblage with respect to the >63 μm one, showing a selective loss of particular ecological groups (stress-tolerant infaunal taxa). Also the better correlation of Foraminiferal Number (FN) and H’ index of >63 μm fraction with Polychlorobiphenyls (PCBs), Polycyclic Aromatic Hydrocarbons (PAHs), Barium (Ba) and Mercury (Hg), demonstrated the higher reliability of this size fraction for environmental assessment purposes.     

Macrobenthos functional groups as indicators of ecological restoration in the northern part of China’s Yellow River Delta Wetlands

Freshwater releases to restore degraded wetlands are a globally recognized way to maintain the biodiversity and enhance the health of wetland ecosystems. To better understand the efficacy of freshwater releases in the northern part of China’s Yellow River Delta Wetlands, we used macrobenthos functional groups in spring (before freshwater releases), summer (during), and autumn (after) as indicators of the ecological responses. We also created abundance–biomass comparison curves and analyzed secondary production of each trophic level to evaluate the magnitude of the disturbance of the macrobenthos community. Abundance, biomass, and biodiversity of macrobenthos functional groups generally improved after the freshwater releases. In contrast with an intertidal (reference) area, the macrobenthos community in the ecological restoration area tended to be freshwater species. In the ecological restoration area, strong and moderate ecological disturbance of the macrobenthos community was evident during and after freshwater releases because the abundance curve remained above the biomass curve. Secondary production was in the order trophic level III > II ≅ IV in the summer, which indicates fragility of the macrobenthos community. The ecological restoration area had the highest sediment total organic carbon and moisture contents, but the lowest salinity and median particle size, and these differences were statistically significant. Our results suggest that adaptive freshwater releases, including a long-term freshwater release plan that more closely emulates natural flows and increasing the efficiency of freshwater utilization, will be necessary to achieve sustainable management of the wetland’s ecosystem and reduce the disturbance caused by the freshwater releases.     

Pollution characteristics of mercury (Hg) in surface sediments of major basins,China

To investigate the pollution status and potential pollution risk of mercury (Hg) in China, surface sediment samples were collected from eight hundred and eighty-one sites, including ten major basins (Songhua River Basin (SRB), Liao River Basin (LRB), Hai River Basin (HRB), Yellow River Basin (YRB), Huai River Basin (HuRB), Yangtze River Basin (YtRB), Pearl River Basin (PRB), Southeastern River Basin (SeRB), Southwestern River Basin (SwRB) and Northwestern River Basin (NwRB)). Results showed that Hg concentrations in sediments of ten basins in China ranged from 0.001 to 8.800 mg/kg, with average ± S.D. value of 0.274 ± 0.675 mg/kg, which was obviously higher than Chinese soil background value (0.038 mg/kg) and Chinese sediment background value (0.040 mg/kg). The mean Hg concentration of ten basins decreased in the order of HRB > YtRB > SRB > PRB > HuRB > SwRB > YRB > SeRB > LRB > NwRB. Moreover, it was found that the Hg concentrations in the sediments of LRB, YtRB, PRB, SeRB and SwRB were partly driven by their total organic carbon (TOC) contents, while the effect of pH on the distribution of Hg was not obvious. The Hg concentration data were also compared with those got in other periods (1994–2015) to obtain the general variation tendency of Hg level. It was recorded that Hg concentrations in HRB have remained on high levels for a long history, while Hg contamination situation in YRB after 2004 has potentially turned to be better. The results of pollution assessment by sediment quality assessment guidelines (SQGs), contamination factor (CF), geoaccumulation index (I_geo) and potential ecological risk (E_i) suggested that YRB and HRB were the most seriously polluted river basins among the ten basins. It is urgent of constructing SQGs in China to scientifically evaluate the Hg pollution in the future.     

Feeding enrichment in an opportunistic carnivore: The red fox

In captive carnivores, species-specific behaviour is often restricted by inadequate feeding regimens. Feeding live prey is not feasible in most places and food delivery is often highly predictable in space and time which is considerably different from the situation in the wild. As a result, captive carnivores are often inactive, show little behavioural diversity and are prone to behavioural problems such as stereotypic pacing. Using artificial feeding devices to substitute for natural food resources is a way to address these problems. In a group of four red fox (Vulpes vulpes), we compared a conventional feeding method to four different methods through the use of feeding enrichment that were based on natural foraging strategies of opportunistic carnivores. Feeding enrichments consisted of electronic feeders delivering food unpredictable in time which were successively combined with one of the three additional treatments: a self-service food box (allowing control over access to food), manually scattering food (unpredictable in space), and an electronic dispenser delivering food unpredictably both in space and time. The aim of administering feeding enrichment in this study was to stimulate appetitive (food searching) behaviour and to increase time spent in feeding. Compared to conventional feeding, diversity of behaviour and overall activity were significantly enhanced in the presence of electronic feeders in all four foxes (EF > CON1 = CON2, EF + SF > CON1 = CON2, EF + MS > CON1 = CON2, EF + ED > CON1 = CON2). Behavioural diversity was highest when the foxes had control over access to food (EF + SF), while the manual scattering of food (EF + MS) and the electronic dispenser (EF + ED) enhanced food searching behaviour. These results indicate that in opportunistic carnivores natural foraging and feeding behaviour can be stimulated by simple feeding enrichment strategies, and that foraging behaviour is stimulated most when food delivery is unpredictable both in space and time.     

Bioluminescent bioreporter Pseudomonas putida TVA8 as a detector of water pollution. Operational conditions and selectivity of free cells sensor

Conditions influencing bioluminescence output from Pseudomonas putida TVA8 harboring chromosomal tod-luxCDABE fusion were followed. In complex media, cell growth was not influenced by the presence of toluene at 53 mg/L. Bioluminescence induction was tested in minimal medium. At 15 °C the highest bioluminescence induced with toluene (1.325 mg/L) was reached after 6 h. At 25 °C the bioluminescence maximum was approximately 20% lower but this was reached after 3.5 h, and at temperatures of 7 °C, 28 °C, 30 °C and 34 °C, bioluminescence peaked at ≤60% of the maximum. Time courses of bioluminescence were dependent on cell concentrations. The heights of bioluminescence maxima were proportional to toluene concentration in the range 0–26 mg/L. Twenty-three organic pollutants (10³× diluted saturated solutions) were tested as bioluminescent inducers. The bioluminescence maximum decreased in the order: ethylbenzene > toluene > phenol > benzene > 4-ethyltoluene > 4-fluorotoluene > cumene > isobutylbenzene > styrene > trichloroethylene > o-, p-xylene > cresol > m-xylene > 2-methylnaphthalene > benzylchloride > naphthalene > salicylic acid > hexachlorobenzene > 2-chloronaphthalene > biphenyl > 2-bromonaphthalene > 1,3,5-triethylbenzene. Bioluminescence was also induced with ethanol and methanol and the presence of these alcohols in concentrations of ≤1% increased bioluminescence of toluene. The induction of bioluminescence from samples of wastewater and groundwater contaminated with BTEX (benzene, toluene, ethylbenzene and xylene) from 0.5 to 120 mg/L was demonstrated.