降雨和土壤湿度对贵州旱田土壤N2O释放的影响

以南方亚热带代表性旱田土壤-贵州玉米-油菜轮作田、大豆-冬小麦轮作田和休耕地为观测对象,研究土壤N2O释放通量季节变化与降雨和土壤湿度的关系,同时,采用DNDC模型定量探讨了未来降雨量变化对土壤N2O释放的潜在影响,结果表明,降雨与N2O释放峰间存在明显的驱动-响应关系,N2O释放通量与降雨量和土壤湿度间存在正相关性,模型检验结果表明,夏秋季土壤N2O释放通量与降雨量变化呈正相关,而降雨量的大幅度增加或下降将引起冬春季土壤N2O释放通量的微弱下降。     

Stimulation of NO and N2O emissions from soils by SO2 deposition

Sulfur dioxide (SO₂) in the atmosphere has been demonstrated to have many adverse impacts on the environment and human health. In this study, deposition of SO₂ ranging from 9.0 to 127.8 mg kg^?1 with an average of 35.7 mg S kg^?1 was found to substantially stimulate NO and N₂O emissions from soils in the humid subtropical areas of Hainan, Fujian, Jiangxi, and Yunnan provinces of China under field conditions. Laboratory tests indicated that the stimulations were mediated biologically as the effects were not observed in sterilized soils. Acidification of soil resulting from SO₂ deposition was not responsible for the stimulated NO and N₂O emissions alone as the stimulation did not occur by acidifying soil with HNO₃ treatment. By using the ¹⁵N tracing method, we found that the N₂O emissions stimulated by SO₂ deposition were from either denitrification, heterotrophic nitrification or both, but not from autotrophic nitrification. Therefore, atmospheric SO₂ deposition would most likely stimulate NO and N₂O emissions in acidic soils in which heterotrophic nitrification dominates NO and N₂O production and waterlogged soils in which denitrification dominates NO and N₂O production.     

Multivariate regulation of soil CO2 and N2O pulse emissions from agricultural soils

Climate and land‐use models project increasing occurrence of high temperature and water deficit in both agricultural production systems and terrestrial ecosystems. Episodic soil wetting and subsequent drying may increase the occurrence and magnitude of pulsed biogeochemical activity, affecting carbon (C) and nitrogen (N) cycles and influencing greenhouse gas (GHG) emissions. In this study, we provide the first data to explore the responses of carbon dioxide (CO₂) and nitrous oxide (N₂O) fluxes to (i) temperature, (ii) soil water content as percent water holding capacity (%WHC), (iii) substrate availability throughout, and (iv) multiple soil drying and rewetting (DW) events. Each of these factors and their interactions exerted effects on GHG emissions over a range of four (CO₂) and six (N₂O) orders of magnitude. Maximal CO₂ and N₂O fluxes were observed in environments combining intermediate %WHC, elevated temperature, and sufficient substrate availability. Amendments of C and N and their interactions significantly affected CO₂ and N₂O fluxes and altered their temperature sensitivities (Q₁₀) over successive DW cycles. C amendments significantly enhanced CO₂ flux, reduced N₂O flux, and decreased the Q₁₀ of both. N amendments had no effect on CO₂ flux and increased N₂O flux, while significantly depressing the Q₁₀ for CO₂, and having no effect on the Q₁₀ for N₂O. The dynamics across DW cycles could be attributed to changes in soil microbial communities as the different responses to wetting events in specific group of microorganisms, to the altered substrate availabilities, or to both. The complex interactions among parameters influencing trace gas fluxes should be incorporated into next generation earth system models to improve estimation of GHG emissions.     

Linking soil N2O emissions with soil microbial community abundance and structure related to nitrogen cycle in two acid forest soils

Plant and Soil - Tree species and seasonal change influence N2O flux and microbial communities, but the mechanisms are unclear. We studied N2O flux in soils planted with slash pine and oil-seed...     

Reductive soil disinfestation (RSD) alters gross N transformation rates and reduces NO and N2O emissions in degraded vegetable soils

Background and aims

Continuous vegetable cultivation in greenhouses can easily induce soil degradation, which considerably affects the development of sustainable vegetable production. Recently, the reductive soil disinfestation (RSD) is widely used as an alternative to chemical soil disinfestations to improve degraded greenhouse vegetable soils. Considering the importance of nitrogen (N) for plant growth and environment effect, the internal N transformation processes and rates should be well investigated in degraded vegetable soils treated by RSD, but few works have been undertaken.

Methods

Three RSD-treated and three untreated degraded vegetable soils were chosen and a ¹⁵?N tracing incubation experiment differentially labeled with ¹⁵NH₄NO₃ or NH₄ ¹⁵NO₃ was conducted at 25 °C under 50 % water holding capacity (WHC) for 96 h. Soil gross N transformation rates were calculated using a ¹⁵?N tracing model combined with Markov Chain Monte Carlo Metropolis algorithm (Müller et al. 2007), while the emissions of N₂O and NO were also measured.

Results

RSD could significantly enhance the soil microbial NH₄ ⁺ immobilization rate, the heterotrophic and autotrophic nitrification rates, and the NO₃ ^? turnover time. The ratio of heterotrophic nitrification to total inorganic N supply rate (mineralization + heterotrophic nitrification) increased greatly from 5.4 % in untreated vegetable soil to 56.1 % in treated vegetable soil. In addition, low release potential of NO and N₂O was observed in RSD-treated vegetable soil, due to the decrease in the NO and N₂O product ratios from heterotrophic and autotrophic nitrifications. These significant differences in gross N transformation rates, the supply processes and capacity of inorganic N, and the NO and N₂O emissions between untreated and treated vegetable soils could be explained by the elimination of accumulated NO₃ ^?, increased pH, and decreased electrical conductivity (EC) caused by RSD. Noticeably, the NO₃ ^? consumption rates were still significantly lower than the NO₃ ^? production rates in RSD-treated vegetable soil.

Conclusions

Except for improving soil chemical properties, RSD could significantly alter the supply processes of inorganic N and reduce the release potential of N₂O and NO in RSD-treated degraded vegetable soil. In order to retard the re-occurrence of NO₃ ^? accumulation, acidification and salinization and to promote the long-term productivity of greenhouse vegetable fields, the rational use of N fertilizer should be paid great attention to farmers in vegetable cultivation.     

Modeling indirect N2O emissions along the N cascade from cropland soils to rivers

The frequently observed discrepancy between estimations of N₂O emissions at regional or global scale based either on field data or inventories (bottom-up) or on direct atmospheric observations (top-down) suggests that riparian areas and river surfaces play a significant role as hot spots of emission. We developed a modeling procedure to assess N₂O emissions occurring during the transfer of water masses from the subroot water pool of the watershed to the outlet of the river drainage network, including their passage through riparian wetlands. The model was applied to three river basins of increasing size located in the sedimentary geological area of the Paris basin (France) and validated by its capability to predict river N₂O concentrations and fluxes across the river–atmosphere interface. At the scale of the Seine watershed, indirect emissions, i.e. emissions linked to agricultural practices but occurring elsewhere than directly at the field plot, are estimated to represent approximately 20% of the direct emissions from the watershed soils, in good agreement with previous estimates based on empirical accounting approaches. Denitrification in riparian zones is responsible for the largest share of these indirect emissions. The model results are very sensitive to the value of the ratio of N₂O versus (N₂ + N₂O), in the final products of denitrification in rivers and wetlands. By calibration on river N₂O concentrations, a value of 0.015 ± 0.05 is proposed for this ratio, in agreement with recent studies. This represents the main uncertainty factor of the model. In basins with conditions prone to increasing the value of this ratio, higher proportions of indirect N₂O emissions might possibly be observed.

     

Responses of soil N2O emissions to experimental warming regulated by soil moisture in an alpine steppe

土壤氧化亚氮(N₂O)排放是大气N₂O不可忽视的来源。然而, 目前学术界在气候变暖对土壤N₂O排放影响方面的认识仍存在较大争议, 且调控土壤N₂O排放的微生物机制尚不明确。为此, 该研究以青藏高原高寒草原生态系统为研究对象, 使用透明开顶箱(OTCs)模拟气候变暖, 并基于静态箱法测定了2014和2015年生长季(5-10月)的土壤N₂O通量, 同时利用定量PCR技术测定了表层(0-10 cm)土壤中氨氧化古菌(AOA)和氨氧化细菌(AOB)的基因丰度。结果显示: 增温处理导致2014和2015年生长季表层(0-10 cm)土壤温度分别升高了1.7 ℃和1.6 ℃, 土壤体积含水量下降了2.5%和3.3%, 其他的土壤理化性质没有发生显著变化。土壤N₂O通量呈现年际差异, 2014和2015年生长季的平均值分别为3.23和1.47 μg·m ^-2·h ^-1, 然而, 增温处理并没有显著改变土壤N₂O通量。2014年生长季主导硝化作用的AOA和AOB的基因丰度分别为5.0 × 10 ⁷和4.7 × 10 ⁵拷贝·g ^-1, 2015年为15.2 × 10 ⁷和10.0 × 10 ⁵拷贝·g ^-1。尽管基因丰度存在显著的年际差异, 但在两年中与对照相比并未发生显著变化。在生长季尺度上, 增温导致的土壤N₂O变化量与其引起的土壤水分变化量之间显著正相关, 而与土壤温度的变化量之间没有显著相关关系。以上结果表明, 增温导致的土壤干旱会抑制土壤N₂O通量对增温的响应, 意味着未来评估气候变暖情景下土壤N₂O排放量时需考虑增温引发的土壤干旱等间接效应。     

Drainage increases CO2 and N2O emissions from tropical peat soils

Tropical peatlands are vital ecosystems that play an important role in global carbon storage and cycles. Current estimates of greenhouse gases from these peatlands are uncertain as emissions vary with environmental conditions. This study provides the first comprehensive analysis of managed and natural tropical peatland GHG fluxes: heterotrophic (i.e. soil respiration without roots), total CO₂ respiration rates, CH₄ and N₂O fluxes. The study documents studies that measure GHG fluxes from the soil (n = 372) from various land uses, groundwater levels and environmental conditions. We found that total soil respiration was larger in managed peat ecosystems (median = 52.3 Mg CO₂ ha^?1 year^?1) than in natural forest (median = 35.9 Mg CO₂ ha^?1 year^?1). Groundwater level had a stronger effect on soil CO₂ emission than land use. Every 100 mm drop of groundwater level caused an increase of 5.1 and 3.7 Mg CO₂ ha^?1 year^?1 for plantation and cropping land use, respectively. Where groundwater is deep (≥0.5 m), heterotrophic respiration constituted 84% of the total emissions. N₂O emissions were significantly larger at deeper groundwater levels, where every drop in 100 mm of groundwater level resulted in an exponential emission increase (exp(0.7) kg N ha^?1 year^?1). Deeper groundwater levels induced high N₂O emissions, which constitute about 15% of total GHG emissions. CH₄ emissions were large where groundwater is shallow; however, they were substantially smaller than other GHG emissions. When compared to temperate and boreal peatland soils, tropical peatlands had, on average, double the CO₂ emissions. Surprisingly, the CO₂ emission rates in tropical peatlands were in the same magnitude as tropical mineral soils. This comprehensive analysis provides a great understanding of the GHG dynamics within tropical peat soils that can be used as a guide for policymakers to create suitable programmes to manage the sustainability of peatlands effectively.     

Regulatory effects of soil properties on background N2O emissions from agricultural soils in China

The background nitrous oxide (N₂O) emission (BNE) from agricultural soils originates from microbial nitrification and denitrification processes of soil nitrogen supplies, excluding emissions from nitrogen fertilizers applied in the current year. It is of great necessity to quantify BNE accurately at various spatial scales since BNE contributes considerably to the overall N₂O emissions from croplands. Annual BNE rates across various soil/climate regions and major cropping systems of China were determined by network observations during 2002–2006 using the static chamber technique. The observations show BNE rates ranging from 0.1 to 3.67 kg N ha⁻¹ year⁻¹, with a mean of 1.35 kg N ha⁻¹ year⁻¹. Empirical functions are derived for cultivated mineral soils and describe the dependences of annual BNE rates upon soil total nitrogen (TN) content, soil organic carbon (SOC) content, bulk density (BD) and clay fraction (CF), separately or collectively. These empirical functions provide simple approaches to scale up estimated national/regional BNE inventories using available database of soil properties surveys and cropland area statistics. The national BNE of China is estimated to be 0.114–0.184 Tg (1 Tg = 10¹² g) N year⁻¹ in 2000, with the range being due to the use of different approaches. However, the available observations of annual BNE rates do not cover the entire range of soil properties on a national scale. Further work is needed to verify the empirical models for a complete range of soil types. In addition, a predictive empirical relationship between annual BNE rates and TN or SOC is established for cultivated mineral soil at the global scale. However, the empirical models could not accurately predict the BNE rates of cultivated organic soils. Further studies are required to understand the regulatory effects of soil properties on annual BNE rates of cultivated organic soils.     

Mitigating N2O emissions from agricultural soils with fungivorous mites

Nitrous oxide (N₂O) is an important greenhouse gas and an ozone-depleting substance. Due to the long persistence of N₂O in the atmosphere, the mitigation of anthropogenic N₂O emissions, which are mainly derived from microbial N₂O-producing processes, including nitrification and denitrification by bacteria, archaea, and fungi, in agricultural soils, is urgently necessary. Members of mesofauna affect microbial processes by consuming microbial biomass in soil. However, how microbial consumption affects N₂O emissions is largely unknown. Here, we report the significant role of fungivorous mites, the major mesofaunal group in agricultural soils, in regulating N₂O production by fungi, and the results can be applied to the mitigation of N₂O emissions. We found that the application of coconut husks, which is the low-value part of coconut and is commonly employed as a soil conditioner in agriculture, to soil can supply a favorable habitat for fungivorous mites due to its porous structure and thereby increase the mite abundance in agricultural fields. Because mites rapidly consume fungal N₂O producers in soil, the increase in mite abundance substantially decreases the N₂O emissions from soil. Our findings might provide new insight into the mechanisms of soil N₂O emissions and broaden the options for the mitigation of N₂O emissions.Subject terms: Climate-change ecology, Climate-change ecology     

Soil invertebrate fauna affect N2O emissions from soil

Nitrous oxide (N₂O) emissions from soils contribute significantly to global warming. Mitigation of N₂O emissions is severely hampered by a lack of understanding of its main controls. Fluxes can only partly be predicted from soil abiotic factors and microbial analyses – a possible role for soil fauna has until now largely been overlooked. We studied the effect of six groups of soil invertebrate fauna and tested the hypothesis that all of them increase N₂O emissions, although to different extents. We conducted three microcosm experiments with sandy soil and hay residue. Faunal groups included in our experiments were as follows: fungal‐feeding nematodes, mites, springtails, potworms, earthworms and isopods. In experiment I, involving all six faunal groups, N₂O emissions declined with earthworms and potworms from 78.4 (control) to 37.0 (earthworms) or 53.5 (potworms) mg N₂O‐N m^?2. In experiment II, with a higher soil‐to‐hay ratio and mites, springtails and potworms as faunal treatments, N₂O emissions increased with potworms from 51.9 (control) to 123.5 mg N₂O‐N m^?2. Experiment III studied the effect of potworm density; we found that higher densities of potworms accelerated the peak of the N₂O emissions by 5 days (P < 0.001), but the cumulative N₂O emissions remained unaffected. We propose that increased soil aeration by the soil fauna reduced N₂O emissions in experiment I, whereas in experiment II N₂O emissions were driven by increased nitrogen and carbon availability. In experiment III, higher densities of potworms accelerated nitrogen and carbon availability and N₂O emissions, but did not increase them. Overall, our data show that soil fauna can suppress, increase, delay or accelerate N₂O emissions from soil and should therefore be an integral part of future N₂O studies.     

Importance of soil cover box area for the determination of N2O emissions from arable soils

To obtain nutrients mineralised from organic matter in the soil, plants have to respond to its heterogeneous distribution. We measured the timing of nitrogen uptake by wheat from a localised, ¹⁵N labelled organic residue in soil, as well as the timing of changes in root length density. We calculated the rates of N uptake per unit root length (inflows) for roots growing through the residue and for the whole root system. A stimulated local inflow appeared to be the main mechanism of exploitation of the residue N during the first five days of exploitation. 8% of the N that the plants would ultimately obtain from the residue was captured in this period. Roots then proliferated in the residue. This, together with a rapidly declining N inflow, contributed to the capture, over the next seven days, of 63% of the N that the plants derived from the residue. After that time, massive root proliferation occurred in the residue, but relatively little further N was captured.     

Symbiotic relationships between soil fungi and plants reduce N2O emissions from soil

N₂O is a potent greenhouse gas involved in the destruction of the protective ozone layer in the stratosphere and contributing to global warming. The ecological processes regulating its emissions from soil are still poorly understood. Here, we show that the presence of arbuscular mycorrhizal fungi (AMF), a dominant group of soil fungi, which form symbiotic associations with the majority of land plants and which influence a range of important ecosystem functions, can induce a reduction in N₂O emissions from soil. To test for a functional relationship between AMF and N₂O emissions, we manipulated the abundance of AMF in two independent greenhouse experiments using two different approaches (sterilized and re-inoculated soil and non-mycorrhizal tomato mutants) and two different soils. N₂O emissions were increased by 42 and 33% in microcosms with reduced AMF abundance compared to microcosms with a well-established AMF community, suggesting that AMF regulate N₂O emissions. This could partly be explained by increased N immobilization into microbial or plant biomass, reduced concentrations of mineral soil N as a substrate for N₂O emission and altered water relations. Moreover, the abundance of key genes responsible for N₂O production (nirK) was negatively and for N₂O consumption (nosZ) positively correlated to AMF abundance, indicating that the regulation of N₂O emissions is transmitted by AMF-induced changes in the soil microbial community. Our results suggest that the disruption of the AMF symbiosis through intensification of agricultural practices may further contribute to increased N₂O emissions.     

Divergent terrestrial responses of soil N2O emissions to different levels of elevated CO2 and temperature

Understanding the responses of soil nitrous oxide (N₂O) emissions from terrestrial ecosystems to future CO₂ enrichment and warming is critical for the development of mitigation and adaptation policies. The effects of continuous increase in elevated CO₂ (EC) and elevated temperature (ET) on N₂O emissions are not fully known. We synthesized 209 measurements from 70 published studies and carried out a meta-analysis to examine individual and interactive effects of EC and ET on N₂O emissions from grasslands, croplands and forests. On average, a significant increase of 23% in N₂O emissions was observed under EC across all case studies. EC did not affect N₂O emissions from grasslands or forests, but significantly increased N₂O emissions in croplands by 38%. The extent of ET effects on N₂O emissions was nonsignificant and there was no significant difference in N₂O emission responses among these three terrestrial systems. ET only promoted N₂O emissions in forest by about 32% when ET was less than 2°C. The interactive effect of EC and ET on N₂O emissions was significantly synergistic, showing a greater increase than the sum of the effects caused by EC and ET alone. Our findings indicated that the combination of EC and ET substantially promoted soil N₂O and highlighted the urgent need to explore its mechanisms to better understand N₂O responses under future climate change.     

Effects of tillage, simulated cattle grazing and soil moisture on N2O emissions from a winter forage crop

Nitrous oxide (N₂O) emissions to the atmosphere from grazed pasture can be high, especially from urine-affected areas. When pastoral soils are damaged by animal treading, N₂O emissions may increase. In New Zealand, autumn-sown winter forage crops are often grown as a break-crop prior to re-sowing pasture. When these crops are grazed in situ over winter (as is common in New Zealand) there is high risk of soil damage from animal treading as soil moisture contents are often high at this time of year. Moreover, the risk of soil damage during grazing increases when intensive tillage practices are used to establish these forage crops. Consequently, winter grazed forage crops may be an important source of N₂O emissions from intensive pastoral farming systems, and these emissions may be affected by the type of tillage used to establish them. We conducted a replicated field experiment to measure the effects of simulated cattle grazing (mowing followed by simulated treading and the application of synthetic urine) at three soil moisture contents (< field capacity, field capacity and > field capacity) on measured N₂O emissions from soil under an autumn (March) sown winter forage crop (triticale) established with three levels of tillage intensity: (a) intensive, IT, (b) minimum, MT, or (c) no tillage, NT. In all treatments, bulk density in the top 7.5 cm of the soil was unaffected by treading when simulated grazing occurred at < field capacity. It was increased in the IT plots by 13 and 15% when treading occurred at field capacity and > field capacity, and by 10% in the MT plots trodden at > field capacity. Treading did not significantly increase the bulk density in the NT plots. Emissions of N₂O from the tillage treatments decreased in the order IT > MT > NT. N₂O emissions were greatest from plots that were trodden at > field capacity and least from plots trodden at < field capacity. Simulated treading and urine application increased N₂O emission 2 to 6-fold from plots that had no treading but did receive urine. Urine-amended plots had much greater emissions than plots that had no urine. Overall, the greatest emission of 14.4 kg N ha^?1 over 90 days (1.8% of the total urine N applied) was measured from urine-amended IT plots that were trodden at > field capacity. The N₂O emission from urine-amended NT plots that were trodden at < field capacity was 2.0 kg ha^?1 over 90 days (0.25% of the total urine N applied). Decreasing the intensity of tillage used to establish crops and restricting grazing when soils are wet are two of the most effective ways to minimise the risk of high N₂O emissions from grazed winter forage crops.     

A meta‐analysis of fertilizer‐induced soil NO and combined NO+N2O emissions

Soils are among the important sources of atmospheric nitric oxide (NO) and nitrous oxide (N₂O), acting as a critical role in atmospheric chemistry. Updated data derived from 114 peer‐reviewed publications with 520 field measurements were synthesized using meta‐analysis procedure to examine the N fertilizer‐induced soil NO and the combined NO+N₂O emissions across global soils. Besides factors identified in earlier reviews, additional factors responsible for NO fluxes were fertilizer type, soil C/N ratio, crop residue incorporation, tillage, atmospheric carbon dioxide concentration, drought and biomass burning. When averaged across all measurements, soil NO‐N fluxes were estimated to be 4.06 kg ha^?1 yr^?1, with the greatest (9.75 kg ha^?1 yr^?1) in vegetable croplands and the lowest (0.11 kg ha^?1 yr^?1) in rice paddies. Soil NO emissions were more enhanced by synthetic N fertilizer (+38%), relative to organic (+20%) or mixed N (+18%) sources. Compared with synthetic N fertilizer alone, synthetic N fertilizer combined with nitrification inhibitors substantially reduced soil NO emissions by 81%. The global mean direct emission factors of N fertilizer for NO (EF_NO) and combined NO+N₂O (EF_c) were estimated to be 1.16% and 2.58%, with 95% confidence intervals of 0.71–1.61% and 1.81–3.35%, respectively. Forests had the greatest EF_NO (2.39%). Within the croplands, the EF_NO (1.71%) and EF_c (4.13%) were the greatest in vegetable cropping fields. Among different chemical N fertilizer varieties, ammonium nitrate had the greatest EF_NO (2.93%) and EF_c (5.97%). Some options such as organic instead of synthetic N fertilizer, decreasing N fertilizer input rate, nitrification inhibitor and low irrigation frequency could be adopted to mitigate soil NO emissions. More field measurements over multiyears are highly needed to minimize the estimate uncertainties and mitigate soil NO emissions, particularly in forests and vegetable croplands.     

Soil core method for direct simultaneous determination of N2 and N2O emissions from forest soils

In order to quantify N₂-emissions from a spruce and a beech site at the Höglwald Forest, a new measuring system was developed, that allowed simultaneous, direct determination of N₂- and N₂O-emission with high accuracy (detection limit approx. 10 g N m^–2 h^–1 for N₂ and <1 g for N₂O) using a gas-flow core method. This method requires exchange of the soil atmosphere with an artificial atmosphere, that differs only in that N₂ is substituted by He. The measuring system, the methodology of measurements and validation experiments are described in detail. Due to the huge heterogeneity of denitrification activity in different soil cores taken from our forest sites, no general trends of N₂ and N₂O production in relation to soil moisture and temperature could be demonstrated. Based on reasonable number of measurements, this work gives for the first time an estimate of the magnitude of N₂-losses from temperate forest soils. Both the magnitude of N₂-emissions (spruce: 7.2±0.7 kg N₂-N ha^–1 yr^–1; beech: 12.4±3.1 kg N₂-N ha^–1 yr^–1), as well as the N₂O–N₂ ratio (spruce: 0.136±0.04; beech: 0.52±0.19) were significantly higher for soils from the beech sites as compared to soils from the spruce site. The results suggests that N₂-emissions from N-saturated forest soils, still receiving high loads of atmospheric N-deposition, are approx. 30% of atmospheric N-input at the spruce site, and approx. 50% at the beech site. Our results demonstrate that losses of nitrogen in the form of N₂ cannot be neglected in the context of calculating N-balances for given forest sites.     

不同水分管理下稻田土壤CH4和N2O排放与微生物菌群的关系

采用MPN计数法对黑土(海伦)和草甸棕壤(沈阳)稻田生长季4种微生物菌群数量进行了测定,同时采用封闭式箱法对CH4和N2O通量进行观测,以深入了解稻田生物源温室气体排放的微生物学过程,两地试验田均采用长期淹灌与间歇灌溉两种不同水分管理,对实验结果多元回归分析,结果表明,海伦与沈阳两地稻田两种水分管理条件下CH4通量季节变化与产甲烷菌数季节变化存在极显著正相关关系沈阳稻田生长季CH4通量季节变化与甲烷氧化菌数季节变化具有显著正相关性,间歇灌溉条件下黑土稻田N2O通量与反硝化菌数呈显著性正相关关系,两种水分管理条件下沈阳稻田N2O通量与硝化菌数具有显著正相关关系,间歇灌溉条件下沈阳稻田N2O通量与反硝化菌数呈显著性正相关关系。     

Simultaneous quantification of N2, NH3 and N2O emissions from a flooded paddy field under different N fertilization regimes

Gaseous nitrogen (N) emissions, especially emissions of dinitrogen (N₂) and ammonia (NH₃), have long been considered as the major pathways of N loss from flooded rice paddies. However, no studies have simultaneously evaluated the overall response of gaseous N losses to improved N fertilization practices due to the difficulties to directly measure N₂ emissions from paddy soils. We simultaneously quantified emissions of N₂ (using membrane inlet mass spectrometry), NH₃ and nitrous oxide (N₂O) from a flooded paddy field in southern China over an entire rice‐growing season. Our field experiment included three treatments: a control treatment (no N addition) and two N fertilizer (220 kg N/ha) application methods, the traditional surface application of N fertilizer and the incorporation of N fertilizer into the soil. Our results show that over the rice‐growing season, the cumulative gaseous N losses from the surface application treatment accounted for 13.5% (N₂), 19.1% (NH₃), 0.2% (N₂O) and 32.8% (total gaseous N loss) of the applied N fertilizer. Compared with the surface application treatment, the incorporation of N fertilizer into the soil decreased the emissions of NH₃, N₂ and N₂O by 14.2%, 13.3% and 42.5%, respectively. Overall, the incorporation of N fertilizer into the soil significantly reduced the total gaseous N loss by 13.8%, improved the fertilizer N use efficiency by 14.4%, increased the rice yield by 13.9% and reduced the gaseous N loss intensity (gaseous N loss/rice yield) by 24.3%. Our results indicate that the incorporation of N fertilizer into the soil is an effective agricultural management practice in ensuring food security and environmental sustainability in flooded paddy ecosystems.