Analysis of the eukaryotic community and metabolites found in clay wall material used in the construction of traditional Japanese buildings

Clay wall (tsuchikabe in Japanese) material for Japanese traditional buildings is manufactured by fermenting a mixture of clay, sand, and rice straw. The aim of this study was to understand the fermentation process in order to gain insight into the ways waste biomass can be used to produce useful materials. In this study, in addition to Clostridium, we suggested that the family Nectriaceae and the Scutellinia sp. of fungi were important in degrading cell wall materials of rice straw, such as cellulose and/or lignin. The microorganisms in the clay wall material produced sulfur-containing inorganic compounds that may sulfurate minerals in clay particles, and polysaccharides that give viscosity to clay wall material, thus increasing workability for plastering, and possibly giving water-resistance to the dried clay wall.     

The claryville clay and early glacial drainage in the Cincinnati,Ohio, region

The Claryville Clay is lacustrine sediment that has been preserved in an entrenched meandering valley beyond the maximum extent of glaciation southeast of Cincinnati, Ohio. Deposition of this and related preglacial material occurred when the north-flowing rivers of the region were dammed by an early episode of glaciation. Although the Claryville Clay is pre-Illinoian in age, its “normal” remmant magnetism suggests that deposition occurred within the past 690,000 years.On the basis of variations in clay mineralogy, grain size, and sedimentary structures, three lithologic units of the Claryville Clay are defined. The upward progression of sedimentary characteristics in the sandy to silty clay is interpreted as reflecting the increasing influence of the southward-advancing glacial dam and fluctuations in this ice margin. Retreat of the ice allowed this proglacial lake to drain. Because of drainage changes initiated by overflow from this and other ponded valleys of the region, the Claryville River valley was abandoned, and new drainage routes were established that discharged west into the Old Ohio River watershed near Madison, Indiana, rather than north into the Teays River valley of central Ohio and Indiana. Present-day rivers lie more than 50 m below the floor of the abandoned Claryville River valley.     

黄土高原三趾马红土的观察(英文)

讨论了黄土高原地区红粘土中脊椎动物化石的考察历史,记录了山西和甘肃"三趾马红土"地点的观察结果。红粘土广泛分布于黄土高原地区,但常被误认为是一个描述性术语,因为其中的许多沉积物既非红色,又不以粘土级颗粒为主。不少红粘土序列中含有古土壤,也含有水成沉积物。此次考察包括榆社盆地的经典地点、灵台任家沟和雷家河,以及庆阳和秦安的一些地点。讨论了在任家沟发现的化石、在玉村新发现的小哺乳动物上新五褶兔( Plio-pentalagus) 和在胡家村观察到的上新世潜穴,后两个地点都位于灵台东北面的庆阳地区。观察结果与晚中新世遍布黄土高原、很可能来自降尘的粘土级颗粒的快速堆积吻合。但在一些区域则以河流或湖泊过程为主,红粘土颗粒部分或全部被水成的粗碎屑沉积物代替,没有找到晚中新世-早上新世干旱化的证据,反而支持高生产力的湿润环境假说。局部富集的脊椎动物化石证明三趾马动物群具有很高的陆生生物量。     

Soil composition affects the nesting behavior of blue-tailed bee-eaters (Merops philippinus) on Kinmen Island

The blue-tailed bee-eater (Merops philippinus) is a summer migrant that breeds on Kinmen Island, located off the west coast of Taiwan, about 5 km from the southern coast of mainland China. The aim of this study was to investigate why blue-tailed bee-eaters build their nests in sandy loam and sandy clay loam, but not in clay loam. Soil chemical and physical properties, and mineralogical composition were measured for the different soil types. Clay loam had a significantly lower pH, Na, and base saturation than did sandy loam or sandy clay loam. Clay loam had a significantly higher N, cation-exchange capacity (CEC), K, and free iron (Fe_d) and aluminum oxide (Al_d) contents than the other soil types. Clay loam had significantly lower sand and higher clay content, and higher bulk density and penetration resistance than the other soil types. The correlation coefficients (r ²) between penetration resistance and Fe_d, Al_d, and clay contents were 0.997, 0.848, and 0.779, respectively. Soil strength and compaction are important criteria for bee-eaters nesting-site selection. The lower pH of clay loam would enhance the exchangeable Al and acidity, further increasing the soil aggregation. Thus, it might prevent the bee-eaters from excavating nesting burrows.     

A barrier covering lignified cell walls of barley straw that restricts access by rumen microorganisms

In barley straw, all stem tissues except the chlorenchyma are lignified. Electronmicroscopic investigations employing staining for cell-wall constituents and replica techniques revealed the presence of a tertiary wall covering the secondary wall and a warty layer deposited on the tertiary wall. The tertiary wall was composed of cellulose fibrils orientated in all directions, which were embedded in matrix material. The warty layer was comprised of granules and globules that were in many instances associated with a thin, flat, continuous layer. Though the tertiary wall could be degraded, the warty layer was resistant to degradation by rumen microorganisms. Only where the warty layer was mechanically disrupted could underlying cell-wall material be degraded. Bacteria could also burrow beneath these layers from exposed cell walls at the cut edges of the plant material. The warty layer forms a barrier to cell-wall-degrading bacteria and limits their colonization of straw stem.     

The reproductive cycle in the population of Saccostrea cuccullatta (Born) from the coast of Karachi,Pakistan

Periphytic diatom communities on clay tile and sterilized rock substrates were compared with those on natural substrates. Clay tiles yielded diatom communities with the least variability between replicate samples, and the greatest similarity to the natural substrate assemblage. The advantages of utilizing introduced substrate communities and problems encountered with their simulation of the natural substrate community are discussed.     

秸秆全量还田对稻田土壤溶解有机碳含量和水稻产量的影响

以南粳44为供试材料,在粘土和砂土土壤中,设置麦秸秆不还田和全量还田(6000 kg·hm^-2)及3种施氮量(0、225、300 kg·hm^-2)试验,研究了麦秸秆全量还田的腐解率和有机碳释放量动态变化,及其对稻田0～45 cm土壤溶解有机碳(DOC)含量和水稻产量的影响.结果表明： 麦秸秆还田的前期(0～30 d)其腐解率和有机碳释放量最高,腐解率为35.0%(粘土)和31.7%(砂土),有机碳释放率为34.1%(粘土)和33.1%(砂土);30 d后两者均减小.施用氮肥可显著促进秸秆腐解和有机碳释放量,粘土中麦秸秆腐解率和有机碳释放量明显大于砂土.麦秸秆还田后土壤DOC含量逐渐增加,至25 d达最大值,粘土和砂土分别为60.18和56.62 mg·L^-1,此后逐渐减小并趋于稳定.麦秸秆还田处理15 cm处土壤DOC含量显著高于未还田处理,但两者在30和45 cm处土壤DOC含量差异不显著,说明秸秆还田主要增加了稻田0～15 cm土层DOC含量.与不施氮处理相比,施氮处理土壤DOC含量降低,2种施氮处理间差异不显著.秸秆还田减少了水稻前期分蘖发生量,显著降低了有效穗数,增加了穗粒数、结实率和千粒重,显著提高了水稻产量.     

Genetic transformation of Bacillus subtilis cells in the presence of clay minerals montmorillonite and kaolinite

The effect of the clay minerals montmorillonite and kaolinite on the transformation of competent Bacillus subtilis cells with chromosomal DNA was studied. Clay particles were found to substantially increase the transformation frequency of competent cells, as well as the rate of their spontaneous chromosomal and plasmid transformation. The effect was ascribed to the adsorption of bacterial cells on the surface of mineral particles.     

煤矿固废资源化利用的生态效率与碳减排——以淮北市为例

工业固废的大量堆积产生多种环境危害,工业固废的资源化利用能够节约资源和缓解环境压力。建筑行业是能源消耗和碳排放的主要部门之一,其中建筑材料生产阶段的能耗和碳排放占有重要的地位。粉煤灰、煤矸石是常见的工业固体废物,尤其是在以煤炭为主要能源的地区。粉煤灰、煤矸石资源化利用的途径之一是用于制造新型墙体砖。本文以煤炭资源型城市淮北市的新型墙体砖(粉煤灰砌块、煤矸石砖)和传统墙体砖(粘土砖、粘土多孔砖)为案例,对墙体砖生产过程的生态效率和碳排放进行分析和比较。在淮北市墙体材料行业碳排放不增加的前提下,以最优生态效率为目标,建立线性规划模型,对淮北市4种主要墙体材料的产量进行规划。分析结果表明:新型墙体材料的生态效率高于传统墙体材料;煤矸石砖生产过程的碳排放系数高于传统墙体砖;粉煤灰砌块生产过程的碳排放系数介于粘土砖和粘土多孔砖之间。在淮北市墙体材料行业碳排放不增加的前提下,与现有的产量相比,淮北市应禁止粘土砖的生产,适当减少粘土多孔砖的产量,适当增加粉煤灰砌块和煤矸石砖的产量,以达到最优生态效率。在最优生态效率的情况下,淮北市新型墙体材料煤矸石砖对煤矸石工业固废的利用率将由目前的15.8%增加到25.2%。     

Extracellular enzyme-clay mineral complexes: Enzyme adsorption, alteration of enzyme activity, and protection from photodegradation

Enzymes released extracellularly by micro-organisms have major functions in nutrient acquisition and organic matter degradation. Clay particles, common in many surface waters, can modify enzyme activity. Clay minerals are known to form aggregates with organic molecules, and the formation of enzyme-clay complexes could alter the level of activity. Montmorillonite clay and clay extracted from Elledge Lake (Tuscaloosa, Alabama) basin soil were combined with alkaline phosphatase, glucosidase, protease, and xylosidase solutions to assess adsorption and the effect of this adsorption on enzyme activity. Adsorption to Elledge Lake basin clay decreased alkaline phosphatase activity, and adsorption to montmorillonite was observed for all four enzymes with reductions in enzyme activities. Adsorption of substrate onto clay surfaces resulted in a concentration effect and increased enzyme activity associated with the particles. When enzyme-clay complexes were exposed to natural sunlight there was a decrease in enzyme activity, but this decrease was usually not significantly different from the adsorption only treatment. The formation of enzyme-clay complexes may serve to protect the enzymes from natural in situ photodegradation. The results indicate the complex interactive effects adsorption of enzymes to clay particles can have on the availability and capability of hydrolysis – reduction of enzyme reactivity, storage attached to clay particles with changes in transport and distribution, and protection from photodegradation.     

Effect of the clay minerals montmorillonite and kaolinite on the genetic transformation of competentBacillus subtilis cells

The effect of the clay minerals montmorillonite and kaolinite on the transformation of competentBacillus subtilis cells with chromosomal DNA was studied. Clay particles were found to substantially increase the transformation frequency of competent cells, as well as the rate of their spontaneous chromosomal and plasmid transformation. The effect was ascribed to the adsorption of bacterial cells on the surface of mineral particles.     

Behavior of adenine in Na-montmorillonite exposed to gamma radiation: implications to chemical evolution studies.

Adenine is an important compound in biological systems, such as genetic and energy utilization processes. Adenine is readily formed in prebiotic conditions. Its synthesis and stability in environmental conditions are of paramount importance in chemical evolution processes. Clay minerals might have played an important role in the early Earth. Clays are known to have a high affinity for organic compounds, and they may provide protection to adsorbed molecules against high-energy radiation. The purpose of this work is to testthese assumptions. We study the stability of adenine under irradiation, in aqueous solution and also adsorbed in a clay mineral. The recovery of adenine after a gamma irradiation was higher in the system containing clay in relation to a system without clay. Results show that adenine is readily adsorbed in the clay, and that the clay act as surface protector toward the degradation of adenine by the radiation.     

Direct electrochemistry and electrocatalysis of horseradish peroxidase based on clay-chitosan-gold nanoparticle nanocomposite

Gold nanoparticles stabilized by chitosan (AuCS) were hybridized with exfoliated clay nanoplates through electrostatic interaction. The resulting clay-chitosan-gold nanoparticle nanocomposite (Clay/AuCS) was used to modify glassy carbon electrode (GCE). HRP, a model peroxidase, was entrapped between the Clay/AuCS film and another clay layer. UV-vis spectrum suggested HRP retained its native conformation in the modified film. Basal plane spacing of clay obtained by X-ray diffraction (XRD) indicated that there was an intercalation-exfoliation-restacking process among HRP, AuCS and clay during the modified film drying. The immobilized HRP showed a pair of quasi-reversible redox peaks at -0.195 V (vs. saturated Ag/AgCl electrode) in 0.1M PBS (pH 7.0), and the biosensor displayed a fast amperometric response to H(2)O(2) with a wide linear range of 39 microM to 3.1 mM. The detection limit was 9.0 microM based on the signal to noise ratio of 3. The kinetic parameters such as alpha (charge transfer coefficient), k(s) (electron transfer rate constant) and K(m) (Michaelis-Menten constant) were evaluated to be 0.53, 2.95+/-0.20s(-1) and 23.15 mM, respectively.     

Effect of Soil Texture and the Clay Component on Migration of Meloidogyne incognita Second-stage Juveniles

The vertical migration of M. incognita juveniles introduced at 20 cm from the roots was studied in five natural soils, 100% silica sand, 95% silica sand with 5% clay, 90% silica sand with 10% clay, and 95% silica sand with 5% clay as a concentrated layer. In natural soils the percentage of juveniles capable of migrating 20 cm and penetrating the roots decreased when the percentage of clay and silt increased. No migration occurred in silica sand without clay particles; when 5 or 10% of clay were mixed to silica sand, 34 and 26%, respectively, of the juveniles were able to migrate 20 cm. Clay separated from silica sand in which tomatoes were grown was attractive for juveniles. It is suggested that clay particles aid in the migration of root-knot juveniles over long distances to plant roots by absorbing and holding root exudates or bacterial by-products which form a concentration gradient enabling nematodes to locate roots.     

Effect of ozone and sodium hydroxide treatments on the degradability of cotton straw monosaccharides by rumen microorganisms

Summary Cotton straw (CS) was treated with ozone and sodium hydroxide and the effect of the treatments on the in vitro digestibility of monosaccharides in the whole material and in cell walls was studied. The digestibility of the major components — glucose and xylose in the untreated whole material was low, 26.3 and 14.3%, respectively, whereas that of the minor components was high, in the range of 60–70%. Ozonation resulted in an increase in digestibility of most of the sugars, with a particular effect on glucose and xylose, the digestibility of which was raised to 72 and 67%, respectively. Sodium hydroxide exerted a modest effect, increasing the in vitro digestibility values for glucose to 35.7% and for xylose to 32.3%.The digestibility of glucose, xylose and uronic acids in the cell wall of the untreated material was 19.7, 8.73 and 21.9%, respectively, whereas the values for the minor components ranged between 50 and 60%. The ozone treatment increased the in vitro degradability of the residual glucose, xylose and uronic acids to 63.7, 26.3 and 53.5%, respectively.There was a lag time of between 12 and 24 h before the rumen bacteria started to hydrolyse the cell wall glucose, xylose and uronic acids. The lag time for those cell wall sugars in the ozonated CS was the longest (24 h) but their rate of in vitro digestion during the last 24 h was higher in the ozonated than in the untreated or sodium hydroxide-treated cotton straw. The practical implications of the above-mentioned findings are discussed.     

Mineralogy and biogeochemistry of potassium in the Skogaby experimental forest,southwest Sweden: pools,fluxes and K/Rb ratios in soil and biomass

Clay minerals and K feldspars were evaluated as sources of K in a Norway spruce stand (Picea abies (L.) Karst.) from the Skogaby experimental forest in southwest Sweden. The soil, developed in a Quaternary glacial till, has only 3–5% clay, and more than 95% of its K resides in feldspars. Ratios of K/Rb were assessed in interlayers of 2:1 clay minerals (extracted with hot (100 °C) 2 M HCl), biomass and the forest floor. These compartments had similarly low K/Rb ratios, whereas K feldspars were significantly poorer in Rb. A fractionation model indicated preferential retention of Rb in the biomass and forest floor, due to stronger adsorption of Rb than K in the humus, as well as preferential uptake of K from the exchange complex in the mineral soil. Preferential uptake of K may result from weaker adsorption of K by the cation exchanger, or preference for dissolved K over Rb by the roots. A quantitative mineralogical analysis revealed that loss from micas may account for half of the Holocene loss of K from the soil, which was approximately 22 Mg ha^?1. Exceptionally low K/Rb ratios in HCl extracts of the upper 60 cm of the profile indicated extensive loss of K from mica in the parent material and re-fixation of K and Rb at lower ratios. The results indicate that fixation in and release from clay minerals may be prominent in the cycling of K, even in a soil that is poor in clay minerals.     

The effects of long-term fertilization on the accumulation of organic carbon in the deep soil profile of an oasis farmland

Background and aims

Deeper soils represent a poorly understood, but potentially important, sink for carbon sequestration. The objective of this study was to determine the effects of long-term fertilization on soil organic carbon (SOC), its labile fractions and aggregate-associated carbon throughout a 0–3 m soil profile.

Methods

The investigation was conducted in a field experiment started in 1990 in an oasis farmland cropped with winter wheat. The following treatments were compared with the desert from which the oasis was created: CK (no fertilizer), NPK, N2P2K, NPKR, and N2P2R2 (“2” for double fertilizer and “R” for straw residue)

Results

SOC contents increased by 14–56 % in the topsoil (0–0.2 m), but decreased by 15–22 % in the subsoil (0.2–0.6 m) under all fertilizer treatments. In the deep layer (0.6–3 m) there were significant differences between the treatments: SOC decreased by 5–9 % in treatments without straw, but increased by 4–9 % in treatments with straw. Labile fractions (particulate organic carbon and light fraction organic carbon) also showed similar trends. Both the fertilizer and CK treatments led to an increase in the amount of macro-aggregates (>0.25 mm), especially small macro-aggregates (0.25–2 mm), throughout the soil profile. SOC content was highest in the macro-aggregates, intermediate in the silt + clay fraction (<0.053 mm), and lowest in the micro-aggregates (0.25–0.053 mm). However, 44–87 % of total SOC was stored in the silt + clay fraction, especially in the deep layer (at least 80 %).

Conclusions

After 20 years of fertilizer applications, difference in SOC mainly occurred in the deep layer, and preservation of SOC in the silt + clay fraction appeared to be a prerequisite for soil-carbon sequestration. Applying inorganic fertilizer alone decreased SOC content in the silt + clay fraction in the deep layer, while the combined applications with straw resulted in higher SOC content in the silt + clay fraction in that layer, which turned out to be the main mechanism for increasing SOC content. Our study indicated that applying straw with inorganic fertilizer is the best practice for carbon sequestration, which occurred mainly in the deep soil layer.     

Learning outcomes and student-perceived value of clay modeling and cat dissection in undergraduate human anatomy and physiology

Alternatives and/or supplements to animal dissection are being explored by educators of human anatomy at different academic levels. Clay modeling is one such alternative that provides a kinesthetic, three-dimensional, constructive, and sensory approach to learning human anatomy. The present study compared two laboratory techniques, clay modeling of human anatomy and dissection of preserved cat specimens, in the instruction of muscles, peripheral nerves, and blood vessels. Specifically, we examined the effect of each technique on student performance on low-order and high-order questions related to each body system as well as the student-perceived value of each technique. Students who modeled anatomic structures in clay scored significantly higher on low-order questions related to peripheral nerves; scores were comparable between groups for high-order questions on peripheral nerves and for questions on muscles and blood vessels. Likert-scale surveys were used to measure student responses to statements about each laboratory technique. A significantly greater percentage of students in the clay modeling group "agreed" or "strongly agreed" with positive statements about their respective technique. These results indicate that clay modeling and cat dissection are equally effective in achieving student learning outcomes for certain systems in undergraduate human anatomy. Furthermore, clay modeling appears to be the preferred technique based on students' subjective perceptions of value to their learning experience.     

Influence of suspended clay on phosphorus uptake by periphyton

We investigated the effect of suspended clay upon the phosphorus uptake rate exhibited by lotic periphyton communities. Suspended inorganic clays and periphyton are common to aquatic environments, and both can strongly influence physical and chemical water conditions. We used replicated artificial stream channels to test the prediction that suspended clay particles would affect the uptake of soluble reactive phosphorus (SRP) by periphyton. Commercially available kaolinite and bentonite clays were characterized for their aqueous suspension behavior and affinities for SRP. Periphyton was grown in a recirculating stream system and subjected to simultaneous suspended clay and SRP additions. SRP removal from solution, both in the presence and absence of suspended clays, was used to quantify SRP uptake parameters by periphyton. Clay type and concentrations of 20, 80, and 200 mg l⁻¹ had no significant effect upon SRP uptake rate exhibited by periphyton during three 90-min experiments. Less than 1% of SRP removal was attributable to the suspended clay load or artificial stream construction materials, based on clay isotherm data and material sorption studies, indicating that 99% of SRP removal was attributable to biotic uptake. Removal of SRP (as KH₂PO₄) was described by a first-order equation with rate constants ranging between 0.02 and 0.14 min⁻¹. Our results suggest that high turbidity conditions caused by suspended mineral clays have little immediate effect upon SRP removal from the water column by periphyton. Handling editor: D. Ryder     

Biocomposite films based on alginate and organically modified clay

Sodium alginate/sodium montmorillonite hybrid films were prepared by casting from the suspension of sodium alginate and different clay samples. Clay samples had been modified with a cationic surfactant, a cationic polymer, and a small polar molecule, respectively. Benzethonium chloride, polyethyleneimine and urea were used as clay modifiers. The composite films begin to disintegrate at a higher temperature and with less weight loss than the pure alginate films. This suggests an enhancement of the film thermal stability due to the modification of the alginate with clay samples.