共查询到13条相似文献,搜索用时 0 毫秒
1.
Sara Gallego Joaquim Vila José María Nieto Mercedes Urdiain Ramon Rosselló-Móra Magdalena Grifoll 《Systematic and applied microbiology》2010
A Gram-negative bacterium designated UBF-P1T was isolated from an enrichment culture established in nutrient supplemented artificial sea water with pyrene as a carbon source, and inoculated with a marine fuel oil-degrading consortium obtained from a sand sample collected from the beach of Corrubedo (A Coruña, Galicia, Spain) after the Prestige accidental oil spill. Phylogenetic analysis based on the almost complete 16S rRNA gene sequence affiliated strain UBF-P1T with the family Cohaesibacteraceae, Cohaesibacter gelatinilyticus (DSM 18289T) being the closest relative species with 92% sequence similarity. Cells were irregular rods, motile, strictly aerobic, catalase and oxidase positive. Ubiquinone 10 was the major respiratory lipoquinone. The major polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), and phosphatidylcholine (PC). The major fatty acids detected were C18:1ω7c, C19:0 cycloω8c, and C16:0. The G + C content of strain UBF-P1T was 63.9 mol%. The taxonomic comparison with the closest relative based on genotypic, phenotypic and chemotaxonomic characteristics supported that strain UBF-P1T could be classified as a novel genus and species, for which the name Breoghania corrubedonensis gen. nov., sp. nov. is proposed. The type strain of this new taxon is UBF-P1T (CECT 7622, LMG 25482, DSM 23382). 相似文献
2.
C. Crespo 《Journal of invertebrate pathology》2009,102(3):214-219
In this work, we describe the occurrence of irregular shaped green aggregations in the mantle, gill filaments, adductor muscle, visceral mass and haemolymph of wild mussels (Mytilus galloprovincialis) collected from the Vigo estuary (Galicia, NW Spain). Microscopic examination of these masses revealed that they consist of intracellular green algae which are spherical to oval in shape, 5 μm in length and 3 μm in width, without flagella and with a smooth surface. The algal cells present a small single nucleus, a mitochondrion, 1-2 parietal chloroplasts and lack pyrenoids. Reproduction is by formation of 2-4 autospores or daughter cells. Pigment analysis reveals the presence of photopigments typical of green algae in addition to alloxanthin, diadinoxanthin and diatoxanthin. These carotenoids are noted for the first time in a parasitic chlorophyte. The signs of infection, together with the morphological observations, suggest that this parasitic algae may be Coccomyxa parasitica. However, further molecular studies are required for confirmation. This is the first report of Coccomyxa algae parasitizing the species M. galloprovincialis. 相似文献
3.
Pter Traj Ali Hazhmat Abdolkhaliq Anett Nmeth Smuel Trisztn Dajcs Ferenc Tmsi Tea Lanisnik-Rizner Istvn Zupk Erzsbet Mernyk 《Journal of enzyme inhibition and medicinal chemistry》2021,36(1):895
Facile syntheses of 3-O-carbamoyl, -sulfamoyl, or -pivaloyl derivatives of 13α-oestrone and its 17-deoxy counterpart have been carried out. Microwave-induced, Ni-catalysed Suzuki–Miyaura couplings of the newly synthesised phenol esters with phenylboronic acid afforded 3-deoxy-3-phenyl-13α-oestrone derivatives. The carbamate and pivalate esters proved to be suitable for regioselective arylations. 2-(4-Substituted) phenyl derivatives were synthesised via Pd-catalysed, microwave-assisted C–H activation reactions. An efficient, one-pot, tandem methodology was elaborated for the introduction of the carbamoyl or pivaloyl group followed by regioselective C-2-arylation and subsequent removal of the directing group. The antiproliferative properties of the novel 13α-oestrone derivatives were evaluated in vitro on five human adherent cancer cell lines of gynaecological origin. 3-Sulfamate derivatives displayed substantial cell growth inhibitory potential against certain cell lines. The newly identified antiproliferative compounds having hormonally inactive core might be promising candidates for the design of more active anticancer agents. 相似文献
4.
Michael Staab 《ZooKeys》2015,(516):137-155
Aenictus is the most species-rich genus of army ants in the subfamily Dorylinae and one of the most species rich ant genera in China and the world. In this paper, a new species of the Aenictus
ceylonicus group, Aenictus
hoelldobleri
sp. n., is described and illustrated based on the worker caste. The new species occurs in the subtropical forests of south-east China and is morphologically most similar to Aenictus
henanensis Li & Wang, 2005 and Aenictus
wudangshanensis Wang, 2006. Aenictus
hoelldobleri
sp. n. can be distinguished from both species by the shape of the subpetiolar process. The new species also resembles Aenictus
lifuiae
Terayama 1984 and Aenictus
thailandianus Terayama & Kubota, 1993 but clearly differs in various features of the cuticular sculpture. A key to the Chinese species of the Aenictus
ceylonicus group based on the worker caste is provided, which may help to reassess and clarify the taxonomic status of the abundant Chinese records of the true Aenictus
ceylonicus (Mayr, 1866), a species which almost certainly does not occur in China. Several new locality records are given, among them the first record of Aenictus
watanasiti Jaitrong & Yamane, 2013 from China. 相似文献
5.
Liang Li Yuan-Yuan Liu Bao-Chen Liu Lu-Ping Zhang 《Memórias do Instituto Oswaldo Cruz》2013,108(1):41-47
Heliconema hainanensis sp. nov. collected from Uroconger lepturus (Richardson) (Anguilliformes: Congridae), Muraenesox cinereus (Forsskål) and Congresox talabonoides (Bleeker) (Anguilliformes: Muraenesocidae) in the South China Sea was described using light and scanning electron microscopy. The new species differs from its congeners by the following morphology: pseudolabia, the number and arrangement of caudal papillae (4 pairs of pedunculate precloacal papillae arranged in 2 groups of 2 and 2 pairs and 6 pairs of pedunculate postcloacal papillae arranged in 4 groups of 1, 2, 1 and 2 pairs), the length of spicules [left spicule 0.51-0.69 mm, right spicule 0.20-0.27 mm, spicule (right:left) ratio 1:2.20-2.69] and the morphology of the female tail tip. In addition, specimens of the new species collected from the three different hosts and specimens of an unidentified species of Heliconema collected from U. lepturus were characterised using molecular methods by sequencing the internal transcribed spacer (ITS) of ribosomal DNA. Analyses and comparison of the ITS sequence of H. hainanensis sp. nov. with Heliconema sp. support the validity of the new species based on morphological observations. An identification key to the species of Heliconema is also provided. 相似文献
6.
LIU Feng-Long ZHANG Hong-BinSHI Ding-Ji SHANG Zhi-Di LIN Chen SHAO Ning PENG GuohongZHANG Xue-Yan ZHANG Hai-Xia WU Jin-Yin XU Xu-Dong WANG JieJIANG Yue-Hua ZHONG Ze-PuZHAO Shu-JinWU Min CENG Cheng-Kui 《中国科学:生命科学英文版》1999,42(1):25-33
The construction of the shuttle, expression vector of human tumor necrosis factor alpha (hTNF-α) gene and its expression in
a cyanobacteriumAnabaena sp. PCC 7120 was reported. The 700-bp hTNF cDNA fragments have been recovered from plasmid pRL-rhTNF, then inserted downstream
of the promoter PpsbA in the plasmid pRL439. The resultant intermediary plasmid pRL-TC has further been combined with the
shuttle vector pDC-8 to get the shuttle, expression vector pDGTNF. The expression of the rhTNF gene inEscherichia coli has been analyzed by SDS-PAGE and thin-layer scanning, and the results show that the expressed TNF protein with these two
vectors is 16.9 percent (pRL-TC) and 15.0 percent (pDC-TNF) of the total proteins in the cells, respectively, while the expression
level of TNF gene in plasmid pRL-rhTNF is only 11.8 percent. Combined with the participation of the conjugal and helper plasmids,
pDC-TNF has been introduced intoAnabaena sp PCC 7120 by triparental conjugative transfer, and the stable transgenic strains have been obtained. The existence of the
introduced plasmid pDC-TNF in recombinant cyanobacterial cells has been demonstrated by the results of the agarose electrophoresis
with the extracted plasmid samples and Southern blotting with α-32P labeled hTNF cDNA probes, while the expression of the hTNF gene inAnabaena sp. PCC 7120 has been confirmed by the results of Western blotting with extracted protein samples and human TNF-alpha monoclonal
antibodies. The cytotoxicity assays using the mouse cancer cell line L929 proved the cytotoxicity of the TNF in the crude
extracts from the transgenic c~anobacteriumAnabaena sp. PCC 7120.
Project supported by the National Natural Science Foundation of China (Grant No. 39280016). 相似文献
7.
Platycarpum vriesendorpiae sp. nov., a second new species of Tribe Henriquezieae (Rubiaceae) from nutrient‐poor soils in the Peruvian Amazon 下载免费PDF全文
The new species Platycarpum vriesendorpiae N. Dávila (Rubiaceae, Henriquezieae) is described from Peru, where it is restricted to white‐sand forests and peatlands in the Tapiche and Blanco River watersheds in southeastern Loreto Region. The new species is generally similar to P. acreanum Rogers of western Brazil in its phyllotaxy, leaf pubescence, and fruit shape and size. However, P. vriesendorpiae differs in its triangular stipules that are 2‐ or 3‐angled at the base and in its smaller corolla. 相似文献
8.
Variation in heat shock protein expression at the latitudinal range limits of a widely‐distributed species,the Glanville fritillary butterfly (Melitaea cinxia) 下载免费PDF全文
Nikhil K. Advani Carly D. Kenkel Sarah W. Davies Camille Parmesan Michael C. Singer Mikhail V. Matz 《Physiological Entomology》2016,41(3):241-248
Studies of heat shock response show a correlation with local climate, although this is more often across altitudinal than latitudinal gradients. In the present study, differences in constitutive but not inducible components of heat shock response are detected among populations of the Glanville fritillary butterfly Melitaea cinxia L. that exist at the species' latitudinal range limits (Finland and Spain). The study demonstrates that macroclimatic differences between these sites should cause greater exposure of the Spanish population to higher temperatures. Thermal stress treatments are used to estimate differences in the expression of four genes potentially relevant for tolerating these temperatures. For the analysis, three heat‐shock proteins and glyceraldehyde‐3‐phosphate dehydrogenase (G3PDH), a glycolysis enzyme that also modulates cell growth based on metabolic state, are chosen. Two constitutive differences are found between the sites. First, insects from Spain have higher levels of Hsp 21.4 than those from Finland regardless of thermal stress treatment; this protein is not inducible. Second, insects from Finland have higher levels of G3PDH. The two remaining Hsps, Hsp20.4 and Hsp90, show dramatic up‐regulation at higher temperatures, although there are no significant differences between insects from the different populations in either constitutive levels or inducibility. In nature, differences between the study populations likely occur in the expression of all four genes that were studied, although these differences would be directly climate‐induced in Hsp20.4 and Hsp90 and constitutive in Hsp21.4 and G3PDH. Inducibility may mitigate the need for constitutive variation in traits that adapt insects to local climate. 相似文献
9.
T. Moura I. Figueiredo† P. BordaloMachado† C. Almeida† L. S. Gordo‡ 《Journal of fish biology》2005,67(3):742-751
A new chimaerid species closely related to Hydrolagus pallidus is described from 13 specimens captured on the Portuguese continental slope (north‐east Atlantic) by commercial longliners at depths of c . 1600 m. The new species is large‐bodied with a rose to light brown body colouration. Hydrolagus lusitanicus n.sp. presents a combination of external morphological characters that allow it to be clearly differentiated from its congeners, in particular, the ratios of pectoral fin length: pectoral fin width and pelvic fin length: pelvic fin width; in having a serrated posterior edge of the dorsal spine; a high number of ridges in the dental plates. Additionally in males, there are differences on the shape and number of hooks in the frontal tenacula, on the number of spines in the prepelvic tenacula and on the length of the pectoral fin margin. 相似文献
10.
11.
Cataglyphis
fisheri
sp. n. is described and illustrated from the United Arab Emirates, Oman and Kingdom of Saudi Arabia based on the worker caste. It belongs to the Cataglyphis
pallida-group which is recorded for the first time from the Arabian Peninsula. Cataglyphis
fisheri
sp. n. is similar to Cataglyphis
pallida Mayr, 1877 from Kazakhstan. Differential diagnosis between these two species is given and a key to the species of the Cataglyphis
pallida-group is presented. Cataglyphis
laylae Collingwood, 2011 is treated as a junior synonym of Cataglyphis
saharae Santschi, 1929. Cataglyphis
flavobrunnea Collingwood & Agosti, 1996 is redescribed and a lectotype for this species is designated. 相似文献
12.
A parasitic wasp of stored‐product pests, Anisopteromalus calandrae (Howard), is known to have a sibling species with a different chromosome number. Here, we report establishment and characterization of an eye color mutant in this sibling species. The phenotype of the mutant is red eye in adults, and crossing experiments revealed that the mutant phenotype is recessive to wild type (brown eye color). We also report DNA barcode sequences (a partial sequence of mitochondrial cytochrome‐c oxidase subunit I) of A. calandrae and the sibling species to enable accurate identification of these morphologically similar species. Analyses of our laboratory strains showed that 12.6% of the analyzed sequences (82 of 652 bp) differed between the two species. Finally, we note that the seed beetle Callosobruchus chinensis (Linné) (family Bruchidae), host of our laboratory strains of the sibling species, is a new record of alternative host at the family‐level for the wasp (known hosts: Anobiidae (natural host) and Curculionidae (alternative host)). 相似文献