Measurement of the latent period of the response of the turtle horizontal retinal cell to electrical stimulation

We have measured the latent period of the depolarization reaction occurring in horizontal cells in response to short bursts of current sent through the retina (anode on sclera, cathode in vitreous). The latent period varied in different experiments from 3 to 7 msec. It is thought that the current acts directly on the presynaptic membrane of the receptors, and that the measured value of the latent period comprises the time of synaptic delay in the transmission of the signal from the receptors to the horizontal cells. The value of the latent period is close to the time interval between the onset of the distal and proximal subcomponents of P_III, as measured by Murakami et al [7, 8], an interval which probably represents time between the onset of the receptor potential and the development of the electrical response of the cells of the inner nuclear layer.Institute of Problems of Transmission of Information, Academy of Sciences of the USSR, Moscow. Translated from Neirofiziologiya, Vol. 2, No. 1, pp. 87–90, January–February, 1970.     

Resonant response of a neural model and of limulus ommatidia to double frequency stimulation

Signals which are the sum of two sinusoids of frequencies v ₁ and v ₂ are used to stimulate: i) an electronic analog of the leaky integrator neural model, ii) the visual neurons of the Limulus lateral eye. This makes it possible to investigate the resonant amplification of the impulse density modulation for v ₁+v ₂ which approaches the free-run discharge rate; this resonance is predicted by the Volterra series representation of the leaky integrator (Poggio and Torre, 1977). The resonant responses obtained look very similar for the simulated discharge and for the experimental one.     

The relationship between signal response selectivity and the functional structure of complex retinal neural networks

In this paper we simulate the simultaneous responses of 45 neurones within a hypothetical retina to a variety of spatio-temporal signals. Our results demonstrate how specific convergent and divergent excitatory and inhibitory connections are sufficient to determine signal response selectivity of specific ganglion cells and so delimit the known types of center-surround receptive fields.This work was partially supported by the Natural Sciences and Engineering Research Council of Canada under Grant A-4345 to M.N.O. and Grant A-4395 to T.M.C. through the University of Alberta     

Polyphosphoinositide hydrolysis in response to light stimulation of rat and chick retina and retinal rod outer segments

Phosphoinositides of chick and rat retina were labelled with [3H]inositol. Exposure of retinal preparations to light for 30 s caused loss of labelled phosphatidylinositol 4,5-bisphosphate and to a smaller extent of the other phosphoinositides. Similar light-induced changes were seen when rod outer segment preparations were used and, when these were illuminated in calcium-free media, phosphatidylinositol 4,5-bisphosphate was the only lipid affected. No inositol 1,4,5-trisphosphate was seen after either 30 s or 5 s of illumination of retina or 30 s illumination of rod outer segments. It is concluded that this compound plays no direct part in vertebrate photoreceptor light transduction, though phosphoinositide metabolism might relate to adaptation mechanisms.     

The neural activities of the greater superficial petrosal nerve of the rat in response to chemical stimulation of the palate

A comparison of the integrated responses of the rat's greatersuperficial petrosal (GSP) and chorda tympani (CT) nerves toa number of taste stimuli was studied. The GSP nerve of therat was very responsive to the chemical stimulation of the oralcavity. Among the selected stimuli related to the four basictaste qualities, 0.5 M sucrose produced the greatest neuralresponse in the GSP nerve, whereas, 0.1 M NaCl produced thegreatest in the CT nerve. The GSP nerve integrated responseto 0.5 M sucrose solution was approximately three times as greatin magnitude as that to a 0.1 M NaCl solution. The neural responsemagnitude of the GSP and CT nerves were as follows: GSP nerve;0.5 M sucrose >0.02 M Na-saccharin >0.05 M citric acid>0.1 M NaCl > 0.01 M quinine-HCl. CT nerve; 0.1 M NaCl> 0.05 M citric acid > 0.02 M Na-saccharin > 0.01 Mquinine-HCl >0.5 M sucrose. The response magnitudes of theGSP nerve to 0.3 M chloride salt solutions were: LiCl > CaCl₂> NaCl > NH₄Cl > KCl, whereas the response magnitudesof the CT nerve to the above salts were: LiCl > NaCl >NH₄Cl > CaCl₂ > KCl. All 0.5 M solutions of the selectedsugars (sucrose, rhamnose, galactose, lactose, fructose, -methyl-D-glucoside,xylose, mannose, arabinose, maltose, sorbose and glucose) evokedneural responses in both GSP and CT nerves. The order of theresponse magnitudes of the GSP nerve to the selected sugarswas similar to that of the CT nerve but the absolute magnitudesof the GSP nerve were greater.     

Human nonspecific response to sound stimulation

Basic physiological responses were studied in healthy adult men exposed to various types of sound stimulation (white noise, pure tones, infrasound). The reaction of the cardio-vascular system to noise turned out to be nonuniform. Changes were observed in the patterns of standard electrocardiograms as well as spatial ballistocardiograms and dynamocardiograms. The magnitude of heart rate responses in the initial stage of stimulation showed a clearcut dependence on the level of noise load. In the first phase of adaptation distinct examinee-to-examinee variations were observed in the time to heart rate normalization, depending on the pattern of personality characteristics. Similar variations were also manifested in biochemical responses as well as in the patterns of EEG.     

Polarized monocyte response to cytokine stimulation

Background  

Mononuclear phagocytes (MPs) stand at the crossroads between the induction of acute inflammation to recruit and activate immune effector cells and the downmodulation of the inflammatory process to contain collateral damage. This decision is extensively modulated by the cytokine microenvironment, which includes a broad array of cytokines whose direct effect on MPs remains largely unexplored. Therefore, we tested whether polarized responses of MPs to pathogens are related to the influence of selected cytokines or represent a mandatory molecular switch through which most cytokines operate.     

Deficiency of neuropilin 2 suppresses VEGF-induced retinal neovascularization

Vascular endothelial growth factor (VEGF) plays a central role in the development of ocular neovascularization (NV) and is an excellent target for therapeutic intervention. VEGF acts through several receptors, including VEGF receptor 1, VEGF receptor 2, neuropilin-1 (Npn1), and Npn2, but the exact role of these receptors in the development of retinal NV is unknown. In this study, we investigated the expression of npn2 mRNA during new blood vessel growth in the retina and used npn2 knockout mice to assess the impact of deficiency of Npn2 on retinal NV. The level of npn2 mRNA in the retina increased during retinal vascular development, after exposure to hyperoxia, and after the onset of retinal ischemia. Immunohistochemistry showed colocalization of Npn2 with a vascular marker in retinal NV. Compared with littermate controls, mice deficient in Npn2 had significantly less ischemia-induced retinal NV and very little subretinal NV due to expression of a Vegf transgene. These data suggest that Npn2 facilitates VEGF-induced retinal NV and may constitute a useful target for therapeutic intervention in ocular diseases complicated by NV.     

Neurogenic adaptation contributes to the afferent response to mechanical stimulation

This study aimed to characterize the effect of mechanical stimuli on mesenteric afferent nerve signaling in the isolated rat jejunum in vitro. This was done to determine the effect of mechanical stresses and strains relative to nonmechanical parameters (neurogenic adaptation). Mechanical stimulations were applied to a segment of jejunum from 15 rats using ramp distension with water at three rates of distension, a relaxation test (volume maintained constant from initial pressure of 20 or 40 mmHg), and a creep test (pressure maintained constant). Circumferential stress and strain and the spike rate increase ratio were calculated for evaluation of afferent nerve activity during the mechanical stimulations. Ramp distension evoked two distinct phases of afferent nerve signaling as a function of circumferential stress or strain. Changing the volume distension rate did not change the stress-strain relationship, but faster distension rate increased the afferent firing rate (P < 0.05). In the stress relaxation test, the spike rate declined faster and to a greater extent than the stress. In the creep test, the spike rate declined, despite a small increase in the strain. Three classes of mechanosensitive single-afferent units (low, wide dynamic range, and high threshold units) showed different response profiles against stress and strain. Low-threshold units exhibited a near linear relationship against the strain (R(2) = 0.8095), whereas high-threshold units exhibited a linear profile against the stress (R(2) = 0.9642). The afferent response is sensitive to the distension speed and to the stress and strain level during distension. However, the afferent nerve response is not a simple function of either stress or strain. Nonmechanical time-dependent adaptive responses other than those related to viscoelasticity also play a role.     

The retinal ON-OFF components giving rise to the delayed response

Summary The delayed response from ON-OFF ganglion cells in the frog retina is preceded by a silent period during which strong inhibition occurs. The length of the silent period depends upon the stimulus flash intensity, the background illumination and the instantaneous adaptation level. Using various combinations of paired stimuli, it was concluded that the silent period and delayed response might be produced by the summation of a long lasting inhibitory component and a long lasting excitatory component. The present results suggest that the excitatory component is elicited by decreasing background illumination (light OFF), the inhibitory component by increasing background illumination (light ON).This work was supported by the Deutsche Forschungsgemeinschaft, also by a U. S. Public Health Service Grant NIH 1F 2NB, 24, 455-01, and partially by MRC MA 3858.     

Internal dynamics determine the cortical response to thalamic stimulation

Although spontaneous activity occurs throughout the neocortex, its relation to the activity produced by external or sensory inputs remains unclear. To address this, we used calcium imaging of mouse thalamocortical slices to reconstruct, with single-cell resolution, the spatiotemporal dynamics of activity of layer 4 in the presence or absence of thalamic stimulation. We found spontaneous neuronal coactivations corresponded to intracellular UP states. Thalamic stimulation of sufficient frequency (>10 Hz) triggered cortical activity, and UP states, indistinguishable from those arising spontaneously. Moreover, neurons were activated in identical and precise spatiotemporal patterns in thalamically triggered and spontaneous events. The similarities between cortical activations indicate that intracortical connectivity plays the dominant role in the cortical response to thalamic inputs. Our data demonstrate that precise spatiotemporal activity patterns can be triggered by thalamic inputs and indicate that the thalamus serves to release intrinsic cortical dynamics.     

Short exposure of polymorphonuclear leucocytes to sodium fluoride suppresses the response to fMLP.

Fluoridated dental care products are used to prevent dental decay. Up to now, there are no data available on whether the fluoride (F-) component of these products affects the bactericidal activity of salivary polymorpho-nuclear leucocytes, which are involved in the protection of the oral mucosa against infection. Therefore, after determining the concentration/time profile of F- in mixed saliva of healthy subjects after topical application of 0.5 g of a 1.25% F- containing gel, unstimulated and fMLP-stimulated polymorphonuclear leucocytes (PMNs) were shortly exposed to these F- concentrations and the generation of superoxide and hypochloric acid were measured, as well as the liberation of lysomal enzymes, and correlated with the cellular Ca2+ and cAMP-levels. The results show that F-, at concentrations as retained in saliva, did not activate the oxidative burst in unstimulated PMNs. In fMLP-activated PMNs, F-suppressed the receptor-mediated increase in the oxidative burst and the liberation of fl-glucuronidase by reduction of the availability of extracellular Ca2+ and, thus, the influx of Ca2+ necessary to couple completely the fMLP signal to effector pathways. These F- concentrations neither altered the liberation of Ca2+ from internal stores nor induced a rise in cAMP. The possible clinical consequences of these results for xerostomic patients with respect to the generation of HOSCN/OSCN/SCN in saliva an important non-immune factor for oral health, are dicussed.