共查询到20条相似文献,搜索用时 31 毫秒
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The exoribonuclease XRN4 is a component of the ethylene response pathway in Arabidopsis 总被引:4,自引:0,他引:4
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Potuschak T Vansiri A Binder BM Lechner E Vierstra RD Genschik P 《The Plant cell》2006,18(11):3047-3057
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Arabidopsis FIERY1, XRN2, and XRN3 are endogenous RNA silencing suppressors 总被引:1,自引:0,他引:1
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Gy I Gasciolli V Lauressergues D Morel JB Gombert J Proux F Proux C Vaucheret H Mallory AC 《The Plant cell》2007,19(11):3451-3461
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Small RNAs (smRNAs) encompass several different classes of short noncoding RNAs. Progress in smRNA research and applications has coincided with the advance of techniques to detect them. Next-generation sequencing technologies are becoming the preferred smRNA profiling method because of their high-throughput capacity and digitized results. In our small RNA profiling study using Solexa, we observed serious biases introduced by the 5' adaptors in small RNA species coverage and abundance; therefore, the results cannot reveal the accurate composition of the small RNAome. We found that the profiling results can be significantly optimized by using an index pool of 64 customized 5' adaptors. This pool of 64 adaptors can be further reduced to four smaller index pools, each containing 16 adaptors, to minimize profiling bias and facilitate multiplexing. It is plausible that this type of bias exists in other deep-sequencing technologies, and adaptor pooling could be an easy work-around solution to reveal the "true" small RNAome. 相似文献
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Background
DNA sequencing is used ubiquitously: from deciphering genomes[1] to determining the primary sequence of small RNAs (smRNAs) [2–5]. The cloning of smRNAs is currently the most conventional method to determine the actual sequence of these important regulators of gene expression. Typical smRNA cloning projects involve the sequencing of hundreds to thousands of smRNA clones that are delimited at their 5' and 3' ends by fixed sequence regions. These primers result from the biochemical protocol used to isolate and convert the smRNA into clonable PCR products. Recently we completed a smRNA cloning project involving tobacco plants, where analysis was required for ~700 smRNA sequences[6]. Finding no easily accessible research tool to enter and analyze smRNA sequences we developed Ebbie to assist us with our study. 相似文献14.
Lister R O'Malley RC Tonti-Filippini J Gregory BD Berry CC Millar AH Ecker JR 《Cell》2008,133(3):523-536
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Buhtz A Springer F Chappell L Baulcombe DC Kehr J 《The Plant journal : for cell and molecular biology》2008,53(5):739-749
Systemic signalling is indispensable for the coordination of diverse physiological processes during development, defence and nutrient allocation. Indirect evidence suggests that plant small RNAs (smRNAs) could be involved in long-distance information transfer via the vasculature of the plant. Analyses of the smRNA complements of vascular exudates from oilseed rape ( Brassica napus ) showed that xylem sap is devoid of RNA, whereas phloem sap contained a large number of smRNAs. In addition to 32 annotated microRNAs (miRNAs) from 18 different families that could be identified and approved, a set of unknown smRNAs, predominantly of 21 and 24 nucleotides in length, was obtained, and selected candidates were found to be highly abundant in phloem sap. Moreover, we could demonstrate that the levels of three miRNAs known to respond to nutrient deprivation in non-vascular tissue, miR395 (sulphate), miR398 (copper) and miR399 (phosphate), were increased in phloem sap during the growth of plants under the respective starvation conditions. Interestingly, only mature miRNA molecules were found to be stress responsive, demonstrating that single-stranded sense miRNAs are most likely to represent the physiologically relevant molecules. The strong responses in the phloem suggest a role of miRNAs in systemic information transfer via this long-distance transport system. 相似文献
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Chen X 《Developmental cell》2008,14(6):811-812
RNA silencing is a genome defense mechanism used by many eukaryotic organisms to fight viruses and to control transposable elements. Work by Gregory et al. on Arabidopsis thaliana (in this issue of Developmental Cell) revealed a mechanism whereby the plant protects its endogenous messenger RNAs from undergoing RNA silencing and uncovered an unexpected role of the cap-binding protein ABH1 in miRNA biogenesis. 相似文献